Restriction map for
pFN21AE0476
(Circular) (Six-base) MAPSORT of: pFN21AE0476.seq Check: 2290 from: 1 to: 5440
pFN21AE0476 5440 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2489 2629 2489
Size: 140 5300
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2511 2783 1937
Size: 574 272 4594
Fragments arranged by size:
4594 574 272
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 3503 4887 5155 1632
Size: 431 1440 1384 268 1917
Fragments arranged by size:
1917 1440 1384 431 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 2517 2589 4339 5316 1203
Size: 1314 72 1750 977 1327
Fragments arranged by size:
1750 1327 1314 977 72
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 3183 4755 3183
Size: 1572 3868
ApaI G_GGCC'C
Cuts at: 2019 2172 2019
Size: 153 5287
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2354 2749 2975 3127 5236 1493
Size: 861 395 226 152 2109 1697
Fragments arranged by size:
2109 1697 861 395 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2201 2614 2765 1870
Size: 73 258 413 151 4545
Fragments arranged by size:
4545 413 258 151 73
BamHI G'GATC_C
Cuts at: 2106 2205 2770 3137 2106
Size: 99 565 367 4409
Fragments arranged by size:
4409 565 367 99
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 2296 2761 4216 618
Size: 325 217 627 347 162 465 1455 1842
Fragments arranged by size:
1842 1455 627 465 347 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2019 2172 2702 2759 1391
Size: 628 153 530 57 4072
Fragments arranged by size:
4072 628 530 153 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2704 928
Size: 971 805 3664
Fragments arranged by size:
3664 971 805
BbvCI CC'TCA_GC
Cuts at: 2630 2630
Size: 5440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 2415 1548
Size: 867 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2310 4051 136
Size: 107 122 71 631 605 638 1741 1525
Fragments arranged by size:
1741 1525 638 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2019 2137 2172 2301 3501 4657 1790
Size: 229 118 35 129 1200 1156 2573
Fragments arranged by size:
2573 1200 1156 229 129 118 35
BmgBI CAC'GTC
Cuts at: 1318 2011 2592 1318
Size: 693 581 4166
Fragments arranged by size:
4166 693 581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2340 4100 1239
Size: 1101 1760 2579
Fragments arranged by size:
2579 1760 1101
Bpu10I CC'TnA_GC
Cuts at: 2630 2630
Size: 5440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3565 4430 4728 3565
Size: 865 298 4277
Fragments arranged by size:
4277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2562 3247 3629 4264
Size: 53 83 186 936 1029 685 382 635
Cuts at: 4264 275
Size: 1451
Fragments arranged by size:
1451 1029 936 685 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2663 1186
Size: 1162 315 3963
Fragments arranged by size:
3963 1162 315
BseYI C'CCAG_C
Cuts at: 2018 2191 4643 5139 2018
Size: 173 2452 496 2319
Fragments arranged by size:
2452 2319 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 1163
Size: 573 649 4218
Fragments arranged by size:
4218 649 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2468 3501 3586 4657 2468
Size: 1033 85 1071 3251
Fragments arranged by size:
3251 1071 1033 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2137 2172 2301 2468 2702 2759
Size: 399 229 118 35 129 167 234 57
Cuts at: 2759 3501 3586 4657 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 234 229 167 129
118 85 57 35
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2489 2629 2795 844
Size: 1645 140 166 3489
Fragments arranged by size:
3489 1645 166 140
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 2461 3500 4512 2461
Size: 1039 1012 3389
Fragments arranged by size:
3389 1039 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 2572 1184
Size: 1388 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2106 2205 2770 3137 3522 3539 4991 5077
Size: 134 99 565 367 385 17 1452 86
Cuts at: 5077 5089 5435 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 565 385 367 346 134 99
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2075 2075
Size: 5440
BtgI C'CryG_G
Cuts at: 513 1065 2004 2271 2307 513
Size: 552 939 267 36 3646
Fragments arranged by size:
3646 939 552 267 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2248 2584 2887
Size: 54 1425 50 275 18 418 336 303
Cuts at: 2887 3776 5405 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 418 336 303 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 3370 1353
Size: 1382 635 3423
Fragments arranged by size:
3423 1382 635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2340 3503 4100 4887 5155 1239
Size: 393 431 277 1163 597 787 268 1524
Fragments arranged by size:
1524 1163 787 597 431 393 277 268
EcoO109I rG'GnC_Cy
Cuts at: 2015 2168 2213 2276 2015
Size: 153 45 63 5179
Fragments arranged by size:
5179 153 63 45
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 2784 3030 669
Size: 988 281 673 173 246 3079
Fragments arranged by size:
3079 988 673 281 246 173
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 2517 5316 2517
Size: 2799 2641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2586 10
Size: 54 1425 343 754 2864
Fragments arranged by size:
2864 1425 754 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3398 3757 3916 518
Size: 648 843 1389 359 159 2042
Fragments arranged by size:
2042 1389 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2559 2856 3533 4681 4926 1606
Size: 953 297 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 297 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2271 2307 513
Size: 552 939 267 36 3646
Fragments arranged by size:
3646 939 552 267 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2111 2282 1348
Size: 763 171 4506
Fragments arranged by size:
4506 763 171
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2213 2276 2213
Size: 63 5377
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2015 2168 2015
Size: 153 5287
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2226 2258 2226
Size: 32 5408
PstI C_TGCA'G
Cuts at: 830 2792 830
Size: 1962 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SanDI GG'GwC_CC
Cuts at: 2276 2276
Size: 5440
SapI GCTCTTCn'nnn_
Cuts at: 2735 2735
Size: 5440
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2788 3923 4604 4795 826
Size: 219 1743 1135 681 191 1471
Fragments arranged by size:
1743 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2203 2767 1872
Size: 331 564 4545
Fragments arranged by size:
4545 564 331
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3544 4445 4707 820
Size: 197 926 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2035 2172 2271 2307 2879 513
Size: 552 939 31 137 99 36 572 3074
Fragments arranged by size:
3074 939 572 552 137 99 36 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2419 3467 3484 3637 3822 4267 325
Size: 1255 839 1048 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1048 839 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5400 5432 5400
Size: 32 5408
Tth111I GACn'n_nGTC
Cuts at: 1316 2708 1316
Size: 1392 4048
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2201 2765 1870
Size: 331 564 4545
Fragments arranged by size:
4545 564 331
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PvuII RsrII SacI SacII
SexAI SfiI SfoI SrfI SwaI XcmI