Restriction map for
pFN21AE0470
(Circular) (Six-base) MAPSORT of: pFN21AE0470.seq Check: 2488 from: 1 to: 5608
pFN21AE0470 5608 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3418 278
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2951 1937
Size: 1014 4594
Acc65I G'GTAC_C
Cuts at: 2085 2929 2085
Size: 844 4764
AclI AA'CG_TT
Cuts at: 1563 3735 4108 1563
Size: 2172 373 3063
Fragments arranged by size:
3063 2172 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3671 5055 5323 1632
Size: 2039 1384 268 1917
Fragments arranged by size:
2039 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5411
AflIII A'CryG_T
Cuts at: 1203 2769 4507 5484 1203
Size: 1566 1738 977 1327
Fragments arranged by size:
1738 1566 1327 977
AgeI A'CCGG_T
Cuts at: 4439 4439
Size: 5608
AhdI GACnn_n'nnGTC
Cuts at: 4337 4337
Size: 5608
AleI CACnn'nnGTG
Cuts at: 1166 2534 2654 1166
Size: 1368 120 4120
Fragments arranged by size:
4120 1368 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5608
AlwNI CAG_nnn'CTG
Cuts at: 2807 3351 4923 2807
Size: 544 1572 3492
Fragments arranged by size:
3492 1572 544
ApaLI G'TGCA_C
Cuts at: 3665 4821 3665
Size: 1156 4452
ApoI r'AATT_y
Cuts at: 1493 2903 2917 3143 3295 5404 1493
Size: 1410 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4162 160
Size: 872 3130 1606
Fragments arranged by size:
3130 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
AvaI C'yCGr_G
Cuts at: 1870 1943 2933 1870
Size: 73 990 4545
Fragments arranged by size:
4545 990 73
BamHI G'GATC_C
Cuts at: 2938 3305 2938
Size: 367 5241
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2294 2578 2810 2929
Size: 325 217 627 298 209 284 232 119
Cuts at: 2929 4384 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 298 284 232 217
209 119
BanII G_rGCy'C
Cuts at: 1391 2927 1391
Size: 1536 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2887 2921 3799 3833 1903
Size: 34 950 34 878 34 3678
Fragments arranged by size:
3678 950 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3500 4710 3500
Size: 1210 4398
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5608
BfrBI ATG'CAT
Cuts at: 2505 2505
Size: 5608
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4219 136
Size: 107 122 71 631 605 2547 1525
Fragments arranged by size:
2547 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2664 5603 2664
Size: 2939 2669
BlpI GC'TnA_GC
Cuts at: 2351 3025 2351
Size: 674 4934
Bme1580I G_kGCm'C
Cuts at: 1790 2583 3669 4825 1790
Size: 793 1086 1156 2573
Fragments arranged by size:
2573 1156 1086 793
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5608
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3339 4297 442
Size: 416 53 83 399 24 1922 958 1753
Fragments arranged by size:
1922 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2157 1056
Size: 1101 4507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2759 4268 1239
Size: 1520 1509 2579
Fragments arranged by size:
2579 1520 1509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 2330 3733 4598 4896 2182
Size: 148 1403 865 298 2894
Fragments arranged by size:
2894 1403 865 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4271 882
Size: 617 2772 2219
Fragments arranged by size:
2772 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3399 493
Size: 711 2195 2702
Fragments arranged by size:
2702 2195 711
BsaBI GATnn'nnATC
Cuts at: 5602 5602
Size: 5608
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3415 3797 4432 275
Size: 53 83 186 936 1882 382 635 1451
Fragments arranged by size:
1882 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4041 4439 4713 4860 5271 4041
Size: 398 274 147 411 4378
Fragments arranged by size:
4378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5383
Fragments arranged by size:
5383 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2220 1186
Size: 1034 4574
BseYI C'CCAG_C
Cuts at: 2554 4811 5307 2554
Size: 2257 496 2855
Fragments arranged by size:
2855 2257 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 2475 2820 2881 1163
Size: 573 717 22 345 61 3890
Fragments arranged by size:
3890 717 573 345 61 22
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3058 3819 4847 1540
Size: 275 186 1057 761 1028 2301
Fragments arranged by size:
2301 1057 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 3754 4825 3669
Size: 85 1071 4452
Fragments arranged by size:
4452 1071 85
BsmI GAATG_Cn'
Cuts at: 2189 2710 3119 3212 2189
Size: 521 409 93 4585
Fragments arranged by size:
4585 521 409 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2583 2927 3669 3754 4825 1391
Size: 399 793 344 742 85 1071 2174
Fragments arranged by size:
2174 1071 793 742 399 344 85
BspEI T'CCGG_A
Cuts at: 5271 5271
Size: 5608
BspHI T'CATG_A
Cuts at: 3497 5227 3497
Size: 1730 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2963 844
Size: 1229 890 3489
Fragments arranged by size:
3489 1229 890
BsrBI CCG'CTC
Cuts at: 1394 1823 3495 1394
Size: 429 1672 3507
Fragments arranged by size:
3507 1672 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2820 4103 4277 65
Size: 1183 1572 1283 174 1396
Fragments arranged by size:
1572 1396 1283 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4252 4439 1700
Size: 110 2442 187 2869
Fragments arranged by size:
2869 2442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5608
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5608
BssSI C'ACGA_G
Cuts at: 3668 4680 3668
Size: 1012 4596
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5608
BstBI TT'CG_AA
Cuts at: 4412 4412
Size: 5608
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5608
BstXI CCAn_nnnn'nTGG
Cuts at: 2656 2656
Size: 5608
BstYI r'GATC_y
Cuts at: 1972 2664 2938 3305 3690 3707 5159 5245 5257
Size: 692 274 367 385 17 1452 86 12
Cuts at: 5257 5603 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 692 385 367 346 274 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2298 2649 513
Size: 552 939 294 351 3472
Fragments arranged by size:
3472 939 552 351 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4869
Fragments arranged by size:
4869 683 56
BtsI GCAGTG_nn'
Cuts at: 2463 3124 3918 3938 2463
Size: 661 794 20 4133
Fragments arranged by size:
4133 794 661 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2803 2838 529
Size: 35 2239 35 3299
Fragments arranged by size:
3299 2239 35 35
DraI TTT'AAA
Cuts at: 2482 2912 2482
Size: 430 5178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4615 809
Size: 507 3299 1802
Fragments arranged by size:
3299 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2497 3055
Size: 54 1425 50 275 18 399 268 558
Cuts at: 3055 3944 5573 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 558 399 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3055 1537
Size: 275 1243 4090
Fragments arranged by size:
4090 1243 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3538 1353
Size: 2185 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4195 4569 4715 387
Size: 1064 138 2606 374 146 1280
Fragments arranged by size:
2606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2759 3671 4268 5055 5323 1239
Size: 393 1127 912 597 787 268 1524
Fragments arranged by size:
1524 1127 912 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2917 3295 2917
Size: 378 5230
EcoRV GAT'ATC
Cuts at: 2677 2677
Size: 5608
FspI TGC'GCA
Cuts at: 4114 4114
Size: 5608
HaeII r_GCGC'y
Cuts at: 1678 4755 1678
Size: 3077 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4249 4281 4323 4355 1373
Size: 32 135 32 2677 32 42 32 2626
Fragments arranged by size:
2677 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2610 2952 3198 669
Size: 988 281 672 342 246 3079
Fragments arranged by size:
3079 988 672 342 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5608
HpaI GTT'AAC
Cuts at: 3198 3198
Size: 5608
KpnI G_GTAC'C
Cuts at: 2089 2933 2089
Size: 844 4764
MfeI C'AATT_G
Cuts at: 3207 3207
Size: 5608
MluI A'CGCG_T
Cuts at: 5484 5484
Size: 5608
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2704 4722 4906 1305
Size: 1399 2018 184 2007
Fragments arranged by size:
2018 2007 1399 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2231 10
Size: 54 1425 343 399 3387
Fragments arranged by size:
3387 1425 399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2654 3566 3925 4084 518
Size: 648 1368 120 912 359 159 2042
Fragments arranged by size:
2042 1368 912 648 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 2576 3024 3701 4849 5094 1606
Size: 970 448 677 1148 245 2120
Fragments arranged by size:
2120 1148 970 677 448 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4117
Fragments arranged by size:
4117 939 552
NdeI CA'TA_TG
Cuts at: 387 2603 387
Size: 2216 3392
NheI G'CTAG_C
Cuts at: 1052 2153 1052
Size: 1101 4507
NotI GC'GGCC_GC
Cuts at: 3055 3055
Size: 5608
NsiI A_TGCA'T
Cuts at: 2507 2507
Size: 5608
NspI r_CATG'y
Cuts at: 1132 1229 2282 2773 2966 4511 1132
Size: 97 1053 491 193 1545 2229
Fragments arranged by size:
2229 1545 1053 491 193 97
PasI CC'CwG_GG
Cuts at: 1348 2140 1348
Size: 792 4816
PciI A'CATG_T
Cuts at: 2769 4507 2769
Size: 1738 3870
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5608
PmeI GTTT'AAAC
Cuts at: 2912 2912
Size: 5608
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 5190 5222 3677
Size: 32 1481 32 4063
Fragments arranged by size:
4063 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5608
PsiI TTA'TAA
Cuts at: 3178 3178
Size: 5608
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2105 2137 2105
Size: 32 5576
PstI C_TGCA'G
Cuts at: 830 2017 2960 830
Size: 1187 943 3478
Fragments arranged by size:
3478 1187 943
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5608
SalI G'TCGA_C
Cuts at: 1936 2950 1936
Size: 1014 4594
SbfI CC_TGCA'GG
Cuts at: 2960 2960
Size: 5608
ScaI AGT'ACT
Cuts at: 3856 5568 3856
Size: 1712 3896
SfcI C'TryA_G
Cuts at: 826 1045 2013 2956 4091 4772 4963 826
Size: 219 968 943 1135 681 191 1471
Fragments arranged by size:
1471 1135 968 943 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5608
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5608
SmaI CCC'GGG
Cuts at: 1872 2935 1872
Size: 1063 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2161 2309 3712 4613 4875 820
Size: 197 926 218 148 1403 901 262 1553
Fragments arranged by size:
1553 1403 926 901 262 218 197 148
SnaBI TAC'GTA
Cuts at: 493 3399 493
Size: 2906 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5608
SphI G_CATG'C
Cuts at: 1132 2966 1132
Size: 1834 3774
SspI AAT'ATT
Cuts at: 5 52 3532 5
Size: 47 3480 2081
Fragments arranged by size:
3480 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5608
StyI C'CwwG_G
Cuts at: 513 1065 2004 2699 3047 513
Size: 552 939 695 348 3074
Fragments arranged by size:
3074 939 695 552 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2546 3635 3652 3805 3990 4435 325
Size: 1255 966 1089 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1089 966 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3854 5566 96
Size: 274 80 33 51 1443 1877 1712 138
Fragments arranged by size:
1877 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2638 2670 5568 5600 2638
Size: 32 2898 32 2646
Fragments arranged by size:
2898 2646 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5608
XbaI T'CTAG_A
Cuts at: 2944 2944
Size: 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5608
XmaI C'CCGG_G
Cuts at: 1870 2933 1870
Size: 1063 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3737 2708
Size: 1029 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3416 276
Size: 53 83 186 936 1882 2468
Fragments arranged by size:
2468 1882 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI