Restriction map for
pFN21AE0469
(Circular) (Six-base) MAPSORT of: pFN21AE0469.seq Check: 6987 from: 1 to: 5329
pFN21AE0469 5329 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2479 2479
Size: 5329
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3139 278
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2672 1937
Size: 735 4594
Acc65I G'GTAC_C
Cuts at: 2650 2650
Size: 5329
AclI AA'CG_TT
Cuts at: 1563 3456 3829 1563
Size: 1893 373 3063
Fragments arranged by size:
3063 1893 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2226 3392 4776 5044 1632
Size: 594 1166 1384 268 1917
Fragments arranged by size:
1917 1384 1166 594 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5132
AflIII A'CryG_T
Cuts at: 1203 4228 5205 1203
Size: 3025 977 1327
Fragments arranged by size:
3025 1327 977
AgeI A'CCGG_T
Cuts at: 4160 4160
Size: 5329
AhdI GACnn_n'nnGTC
Cuts at: 2072 4058 2072
Size: 1986 3343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5329
AlwNI CAG_nnn'CTG
Cuts at: 2494 3072 4644 2494
Size: 578 1572 3179
Fragments arranged by size:
3179 1572 578
ApaI G_GGCC'C
Cuts at: 2014 2023 2150 2014
Size: 9 127 5193
Fragments arranged by size:
5193 127 9
ApaLI G'TGCA_C
Cuts at: 3386 4542 3386
Size: 1156 4173
ApoI r'AATT_y
Cuts at: 1493 2638 2864 3016 5125 1493
Size: 1145 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3883 160
Size: 872 2851 1606
Fragments arranged by size:
2851 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
AvaI C'yCGr_G
Cuts at: 1870 1943 2654 1870
Size: 73 711 4545
Fragments arranged by size:
4545 711 73
BamHI G'GATC_C
Cuts at: 2659 3026 2659
Size: 367 4962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 2650 4105 618
Size: 325 217 627 263 600 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 263 217
BanII G_rGCy'C
Cuts at: 1391 2014 2023 2090 2150 2648 1391
Size: 623 9 67 60 498 4072
Fragments arranged by size:
4072 623 498 67 60 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2214 2243 928
Size: 971 315 29 4014
Fragments arranged by size:
4014 971 315 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3520 3554 1903
Size: 34 1583 34 3678
Fragments arranged by size:
3678 1583 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 4431 3221
Size: 1210 4119
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2018 3940
Size: 107 122 71 631 605 337 9 1922
Cuts at: 3940 136
Size: 1525
Fragments arranged by size:
1922 1525 631 605 337 122 107 71
9
BglII A'GATC_T
Cuts at: 2457 5324 2457
Size: 2867 2462
BlpI GC'TnA_GC
Cuts at: 2746 2746
Size: 5329
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2023 2055 2150 3390 4546 1790
Size: 224 9 32 95 1240 1156 2573
Fragments arranged by size:
2573 1240 1156 224 95 32 9
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2066 2315 3060
Size: 416 53 83 399 24 649 249 745
Cuts at: 3060 4018 442
Size: 958 1753
Fragments arranged by size:
1753 958 745 649 416 399 249 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5329
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 3989 1239
Size: 960 1790 2579
Fragments arranged by size:
2579 1790 960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3454 4319 4617 3454
Size: 865 298 4166
Fragments arranged by size:
4166 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3992 882
Size: 617 2493 2219
Fragments arranged by size:
2493 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3120 493
Size: 711 1916 2702
Fragments arranged by size:
2702 1916 711
BsaBI GATnn'nnATC
Cuts at: 5323 5323
Size: 5329
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2068 3136 3518 4153
Size: 53 83 186 936 535 1068 382 635
Cuts at: 4153 275
Size: 1451
Fragments arranged by size:
1451 1068 936 635 535 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3762 4160 4434 4581 4992 3762
Size: 398 274 147 411 4099
Fragments arranged by size:
4099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5104
Fragments arranged by size:
5104 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 2452 2582 1186
Size: 1010 256 130 3933
Fragments arranged by size:
3933 1010 256 130
BseYI C'CCAG_C
Cuts at: 2474 4532 5028 2474
Size: 2058 496 2775
Fragments arranged by size:
2775 2058 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4756
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2779 3540 4568 1540
Size: 275 186 778 761 1028 2301
Fragments arranged by size:
2301 1028 778 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3390 3475 4546 3390
Size: 85 1071 4173
Fragments arranged by size:
4173 1071 85
BsmI GAATG_Cn'
Cuts at: 2840 2933 2840
Size: 93 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2023 2055 2090 2150 2648 3390
Size: 399 224 9 32 35 60 498 742
Cuts at: 3390 3475 4546 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 399 224 85 60
35 32 9
BspEI T'CCGG_A
Cuts at: 4992 4992
Size: 5329
BspHI T'CATG_A
Cuts at: 3218 4948 3218
Size: 1730 3599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 2684 844
Size: 1635 205 3489
Fragments arranged by size:
3489 1635 205
BsrBI CCG'CTC
Cuts at: 1394 1823 3216 1394
Size: 429 1393 3507
Fragments arranged by size:
3507 1393 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3824 3998 65
Size: 1183 2576 174 1396
Fragments arranged by size:
2576 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 3973 4160 1700
Size: 110 290 1873 187 2869
Fragments arranged by size:
2869 1873 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 2409 96
Size: 2313 3016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5329
BssSI C'ACGA_G
Cuts at: 3389 4401 3389
Size: 1012 4317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5329
BstBI TT'CG_AA
Cuts at: 4133 4133
Size: 5329
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5329
BstYI r'GATC_y
Cuts at: 1972 2457 2659 3026 3411 3428 4880 4966 4978
Size: 485 202 367 385 17 1452 86 12
Cuts at: 4978 5324 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 202 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2314 2314
Size: 5329
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 513
Size: 552 939 615 3223
Fragments arranged by size:
3223 939 615 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2054 487
Size: 683 56 828 3762
Fragments arranged by size:
3762 828 683 56
BtsI GCAGTG_nn'
Cuts at: 2845 3639 3659 2845
Size: 794 20 4515
Fragments arranged by size:
4515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5294
DraI TTT'AAA
Cuts at: 2633 2633
Size: 5329
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4336 809
Size: 507 3020 1802
Fragments arranged by size:
3020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2102 2452 2509
Size: 54 1425 50 275 18 272 350 57
Cuts at: 2509 2776 3665 5294 8
Size: 267 889 1629 43
Fragments arranged by size:
1629 1425 889 350 275 272 267 57
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2776 1537
Size: 275 964 4090
Fragments arranged by size:
4090 964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3259 1353
Size: 1906 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3916 4290 4436 387
Size: 1064 138 2327 374 146 1280
Fragments arranged by size:
2327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2226 3392 3989 4776 5044 1239
Size: 393 567 27 1166 597 787 268 1524
Fragments arranged by size:
1524 1166 787 597 567 393 268 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2181 2181
Size: 5329
EcoO109I rG'GnC_Cy
Cuts at: 2010 2019 2146 2147 2010
Size: 9 127 1 5192
Fragments arranged by size:
5192 127 9 1
EcoRI G'AATT_C
Cuts at: 2638 3016 2638
Size: 378 4951
FspI TGC'GCA
Cuts at: 3835 3835
Size: 5329
HaeII r_GCGC'y
Cuts at: 1678 4476 1678
Size: 2798 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 3970 4002 4044
Size: 32 135 32 486 32 1880 32 42
Cuts at: 4044 4076 1373
Size: 32 2626
Fragments arranged by size:
2626 1880 486 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2673 2919 669
Size: 988 281 735 246 3079
Fragments arranged by size:
3079 988 735 281 246
HindIII A'AGCT_T
Cuts at: 748 2385 2444 748
Size: 1637 59 3633
Fragments arranged by size:
3633 1637 59
HpaI GTT'AAC
Cuts at: 2919 2919
Size: 5329
KpnI G_GTAC'C
Cuts at: 2654 2654
Size: 5329
MfeI C'AATT_G
Cuts at: 2928 2928
Size: 5329
MluI A'CGCG_T
Cuts at: 5205 5205
Size: 5329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4443 4627 1305
Size: 3138 184 2007
Fragments arranged by size:
3138 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2454 2511 10
Size: 54 1425 343 622 57 2828
Fragments arranged by size:
2828 1425 622 343 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3287 3646 3805 518
Size: 648 2121 359 159 2042
Fragments arranged by size:
2121 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2745 3422 4570 4815 1606
Size: 1139 677 1148 245 2120
Fragments arranged by size:
2120 1148 1139 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3838
Fragments arranged by size:
3838 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5329
NotI GC'GGCC_GC
Cuts at: 2776 2776
Size: 5329
NspI r_CATG'y
Cuts at: 1132 1229 2687 4232 1132
Size: 97 1458 1545 2229
Fragments arranged by size:
2229 1545 1458 97
PasI CC'CwG_GG
Cuts at: 1348 2014 2090 1348
Size: 666 76 4587
Fragments arranged by size:
4587 666 76
PciI A'CATG_T
Cuts at: 4228 4228
Size: 5329
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5329
PmeI GTTT'AAAC
Cuts at: 2633 2633
Size: 5329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 4911 4943 3398
Size: 32 1481 32 3784
Fragments arranged by size:
3784 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5329
PsiI TTA'TAA
Cuts at: 2899 2899
Size: 5329
PspOMI G'GGCC_C
Cuts at: 2010 2019 2146 2010
Size: 9 127 5193
Fragments arranged by size:
5193 127 9
PstI C_TGCA'G
Cuts at: 830 2346 2681 830
Size: 1516 335 3478
Fragments arranged by size:
3478 1516 335
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5329
SalI G'TCGA_C
Cuts at: 1936 2671 1936
Size: 735 4594
SbfI CC_TGCA'GG
Cuts at: 2681 2681
Size: 5329
ScaI AGT'ACT
Cuts at: 3577 5289 3577
Size: 1712 3617
SfcI C'TryA_G
Cuts at: 826 1045 2244 2342 2677 3812 4493 4684 826
Size: 219 1199 98 335 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 681 335 219 191 98
SfiI GGCCn_nnn'nGGCC
Cuts at: 2018 2018
Size: 5329
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5329
SmaI CCC'GGG
Cuts at: 1872 2656 1872
Size: 784 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3433 4334 4596 820
Size: 197 926 1490 901 262 1553
Fragments arranged by size:
1553 1490 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3120 493
Size: 2627 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5329
SphI G_CATG'C
Cuts at: 1132 2687 1132
Size: 1555 3774
SspI AAT'ATT
Cuts at: 5 52 3253 5
Size: 47 3201 2081
Fragments arranged by size:
3201 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5329
StyI C'CwwG_G
Cuts at: 513 1065 2004 2524 2768 513
Size: 552 939 520 244 3074
Fragments arranged by size:
3074 939 552 520 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3356 3373 3526 3711 4156 325
Size: 1255 1776 17 153 185 445 1498
Fragments arranged by size:
1776 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2409 3575 5287
Size: 274 80 33 51 1443 432 1166 1712
Cuts at: 5287 96
Size: 138
Fragments arranged by size:
1712 1443 1166 432 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5289 5321 5289
Size: 32 5297
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5329
XbaI T'CTAG_A
Cuts at: 2665 2665
Size: 5329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5329
XmaI C'CCGG_G
Cuts at: 1870 2654 1870
Size: 784 4545
XmnI GAAnn'nnTTC
Cuts at: 3458 3458
Size: 5329
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3137 276
Size: 53 83 186 936 1603 2468
Fragments arranged by size:
2468 1603 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfoI
SrfI SwaI