Restriction map for
pFN21AE0468
(Circular) (Six-base) MAPSORT of: pFN21AE0468.seq Check: 8646 from: 1 to: 5860
pFN21AE0468 5860 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2612 3670 278
Size: 53 83 186 936 1076 1058 2468
Fragments arranged by size:
2468 1076 1058 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 3181 3181
Size: 5860
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2665 2798 3923 5307 5575 1632
Size: 1033 133 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 1033 268 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2229 4759 5736 1203
Size: 1026 2530 977 1327
Fragments arranged by size:
2530 1327 1026 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 2078 4589 2078
Size: 2511 3349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2604 1184
Size: 1420 4440
AlwNI CAG_nnn'CTG
Cuts at: 2075 3603 5175 2075
Size: 1528 1572 2760
Fragments arranged by size:
2760 1572 1528
ApaI G_GGCC'C
Cuts at: 2798 3069 2798
Size: 271 5589
ApaLI G'TGCA_C
Cuts at: 2216 3917 5073 2216
Size: 1701 1156 3003
Fragments arranged by size:
3003 1701 1156
ApoI r'AATT_y
Cuts at: 1493 2286 3169 3395 3547 5656 1493
Size: 793 883 226 152 2109 1697
Fragments arranged by size:
2109 1697 883 793 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 3069 3185 1870
Size: 73 1038 88 116 4545
Fragments arranged by size:
4545 1038 116 88 73
BamHI G'GATC_C
Cuts at: 2037 3190 3557 2037
Size: 1153 367 4340
Fragments arranged by size:
4340 1153 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2367 2493 2546 2630
Size: 325 217 627 510 70 126 53 84
Cuts at: 2630 2883 3098 3181 4636 618
Size: 253 215 83 1455 1842
Fragments arranged by size:
1842 1455 627 510 325 253 217 215
126 84 83 70 53
BanII G_rGCy'C
Cuts at: 1391 2798 3069 3179 1391
Size: 1407 271 110 4072
Fragments arranged by size:
4072 1407 271 110
BbeI G_GCGC'C
Cuts at: 2371 2497 2634 2887 2371
Size: 126 137 253 5344
Fragments arranged by size:
5344 253 137 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2671 928
Size: 971 772 4117
Fragments arranged by size:
4117 971 772
BbvCI CC'TCA_GC
Cuts at: 3025 3025
Size: 5860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3139 3173 4051 4085 1903
Size: 34 1202 34 878 34 3678
Fragments arranged by size:
3678 1202 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3752 4962 3752
Size: 1210 4650
BclI T'GATC_A
Cuts at: 1548 2319 1548
Size: 771 5089
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4471 136
Size: 107 122 71 631 605 2799 1525
Fragments arranged by size:
2799 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2391 5855 2391
Size: 3464 2396
BlpI GC'TnA_GC
Cuts at: 2851 3277 2851
Size: 426 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2798 2982 3069 3103 3122 3921 5077
Size: 430 578 184 87 34 19 799 1156
Cuts at: 5077 1790
Size: 2573
Fragments arranged by size:
2573 1156 799 578 430 184 87 34
19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2969 3152 3591
Size: 416 53 83 399 24 1552 183 439
Cuts at: 3591 4549 442
Size: 958 1753
Fragments arranged by size:
1753 1552 958 439 416 399 183 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2594 3067 4520 1239
Size: 813 542 473 1453 2579
Fragments arranged by size:
2579 1453 813 542 473
Bpu10I CC'TnA_GC
Cuts at: 3025 3025
Size: 5860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2933 3985 4850 5148 2183
Size: 750 1052 865 298 2895
Fragments arranged by size:
2895 1052 865 750 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 2603 2630 4523 882
Size: 617 515 589 27 1893 2219
Fragments arranged by size:
2219 1893 617 589 515 27
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 5854 5854
Size: 5860
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2368 2494 2609 2631
Size: 53 83 186 936 835 126 115 22
Cuts at: 2631 2884 3667 4049 4684 275
Size: 253 783 382 635 1451
Fragments arranged by size:
1451 936 835 783 635 382 253 186
126 115 83 53 22
BsaWI w'CCGG_w
Cuts at: 4293 4691 4965 5112 5523 4293
Size: 398 274 147 411 4630
Fragments arranged by size:
4630 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 2918 2948 1178
Size: 30 165 30 1104 30 381 30 4090
Fragments arranged by size:
4090 1104 381 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2480 1186
Size: 1291 3 4566
Fragments arranged by size:
4566 1291 3
BseYI C'CCAG_C
Cuts at: 2161 2351 2797 2814 5063 5559 2161
Size: 190 446 17 2249 496 2462
Fragments arranged by size:
2462 2249 496 446 190 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3921 4006 5077 2220
Size: 1701 85 1071 3003
Fragments arranged by size:
3003 1701 1071 85
BsmI GAATG_Cn'
Cuts at: 2061 3371 3464 2061
Size: 1310 93 4457
Fragments arranged by size:
4457 1310 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2798 2982 3069 3103 3122 3179
Size: 399 430 578 184 87 34 19 57
Cuts at: 3179 3921 4006 5077 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 430 399 184 87
85 57 34 19
BspEI T'CCGG_A
Cuts at: 5523 5523
Size: 5860
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 3215 844
Size: 1239 1132 3489
Fragments arranged by size:
3489 1239 1132
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 4504 4691 1700
Size: 110 247 2447 187 2869
Fragments arranged by size:
2869 2447 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5860
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3920 4932 3920
Size: 1012 4848
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 794
Size: 1635 4225
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 3001 3040 1184
Size: 1817 39 4004
Fragments arranged by size:
4004 1817 39
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 2037 2134 2391 2568 2716 3190 3557 3942
Size: 65 97 257 177 148 474 367 385
Cuts at: 3942 3959 5411 5497 5509 5855 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 474 385 367 346 257 177
148 97 86 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2293 513
Size: 552 939 168 121 4080
Fragments arranged by size:
4080 939 552 168 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2173 487
Size: 683 56 947 4174
Fragments arranged by size:
4174 947 683 56
BtsI GCAGTG_nn'
Cuts at: 2439 2502 2876 3376 4170 4190 2439
Size: 63 374 500 794 20 4109
Fragments arranged by size:
4109 794 500 374 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2999 4867 809
Size: 507 1683 1868 1802
Fragments arranged by size:
1868 1802 1683 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 3307 4196
Size: 54 1425 50 275 18 192 1285 889
Cuts at: 4196 5825 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1285 889 275 192 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 3790 1353
Size: 761 1676 3423
Fragments arranged by size:
3423 1676 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4447 4821 4967 387
Size: 1064 138 2858 374 146 1280
Fragments arranged by size:
2858 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2594 2665 2798 3067 3923 4520
Size: 393 420 542 71 133 269 856 597
Cuts at: 4520 5307 5575 1239
Size: 787 268 1524
Fragments arranged by size:
1524 856 787 597 542 420 393 269
268 133 71
EcoO109I rG'GnC_Cy
Cuts at: 2072 2773 2794 3065 3066 3091 3153 2072
Size: 701 21 271 1 25 62 4779
Fragments arranged by size:
4779 701 271 62 25 21 1
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2371 2497 2634 2887 2894 5007 1678
Size: 693 126 137 253 7 2113 2531
Fragments arranged by size:
2531 2113 693 253 137 126 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2918 2950 4501
Size: 32 135 32 492 32 822 32 1551
Cuts at: 4501 4533 4575 4607 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1551 822 492 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KasI G'GCGC_C
Cuts at: 2367 2493 2630 2883 2367
Size: 126 137 253 5344
Fragments arranged by size:
5344 253 137 126
KpnI G_GTAC'C
Cuts at: 3185 3185
Size: 5860
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2433 4974 5158 1305
Size: 1128 2541 184 2007
Fragments arranged by size:
2541 2007 1128 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 10
Size: 54 1425 343 192 3846
Fragments arranged by size:
3846 1425 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 3818 4177 4336 518
Size: 648 1368 1284 359 159 2042
Fragments arranged by size:
2042 1368 1284 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2969 3276 3953 5101 5346 1606
Size: 1363 307 677 1148 245 2120
Fragments arranged by size:
2120 1363 1148 677 307 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 5860
NarI GG'CG_CC
Cuts at: 2368 2494 2631 2884 2368
Size: 126 137 253 5344
Fragments arranged by size:
5344 253 137 126
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4369
Fragments arranged by size:
4369 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2128 2207 2233 2539 3218 4763 1132
Size: 97 899 79 26 306 679 1545 2229
Fragments arranged by size:
2229 1545 899 679 306 97 79 26
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 2229 4759 2229
Size: 2530 3330
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2068 2571 2068
Size: 503 5357
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2327 2359 3929 3961 5442 5474 2327
Size: 32 1570 32 1481 32 2713
Fragments arranged by size:
2713 1570 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2773 3153 2072
Size: 701 380 4779
Fragments arranged by size:
4779 701 380
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PspOMI G'GGCC_C
Cuts at: 2794 3065 2794
Size: 271 5589
PstI C_TGCA'G
Cuts at: 830 2264 2446 3212 830
Size: 1434 182 766 3478
Fragments arranged by size:
3478 1434 766 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2969 2969
Size: 5860
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SapI GCTCTTCn'nnn_
Cuts at: 2114 2114
Size: 5860
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 2260 2442 3208 4343 5024 5215 826
Size: 219 1215 182 766 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 766 681 219 191 182
SfoI GGC'GCC
Cuts at: 2369 2495 2632 2885 2369
Size: 126 137 253 5344
Fragments arranged by size:
5344 253 137 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3071 3187 1872
Size: 1199 116 4545
Fragments arranged by size:
4545 1199 116
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2198 2912 3964 4865 5127 820
Size: 197 926 255 714 1052 901 262 1553
Fragments arranged by size:
1553 1052 926 901 714 262 255 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 2128 2207 3218 1132
Size: 996 79 1011 3774
Fragments arranged by size:
3774 1011 996 79
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 2004 2433 2640 2802 2838 3094 3299
Size: 552 939 429 207 162 36 256 205
Cuts at: 3299 513
Size: 3074
Fragments arranged by size:
3074 939 552 429 256 207 205 162
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2365 2386 3887 3904 4057 4242
Size: 1255 588 197 21 1501 17 153 185
Cuts at: 4242 4687 325
Size: 445 1498
Fragments arranged by size:
1501 1498 1255 588 445 197 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4106 5818 96
Size: 274 80 33 51 1443 2129 1712 138
Fragments arranged by size:
2129 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5820 5852 5820
Size: 32 5828
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3069 3185 1870
Size: 1199 116 4545
Fragments arranged by size:
4545 1199 116
XmnI GAAnn'nnTTC
Cuts at: 3989 3989
Size: 5860
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2610 3668 276
Size: 53 83 186 936 1076 1058 2468
Fragments arranged by size:
2468 1076 1058 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI
SwaI