Restriction map for
pFN21AE0466
(Circular) (Six-base) MAPSORT of: pFN21AE0466.seq Check: 9458 from: 1 to: 5710
pFN21AE0466 5710 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2152
Size: 5710
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3053 1937
Size: 1116 4594
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2362 2547 2903 3773 5157 5425 1632
Size: 730 185 356 870 1384 268 1917
Fragments arranged by size:
1917 1384 870 730 356 268 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 4609 5586 1203
Size: 3406 977 1327
Fragments arranged by size:
3406 1327 977
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 2309 4439 2309
Size: 2130 3580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 1184
Size: 1047 4663
AlwNI CAG_nnn'CTG
Cuts at: 2481 2846 2872 3453 5025 2481
Size: 365 26 581 1572 3166
Fragments arranged by size:
3166 1572 581 365 26
ApaLI G'TGCA_C
Cuts at: 2236 3767 4923 2236
Size: 1531 1156 3023
Fragments arranged by size:
3023 1531 1156
ApoI r'AATT_y
Cuts at: 1493 2605 2707 3019 3245 3397 5506 1493
Size: 1112 102 312 226 152 2109 1697
Fragments arranged by size:
2109 1697 1112 312 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 3035 1870
Size: 73 129 963 4545
Fragments arranged by size:
4545 963 129 73
BamHI G'GATC_C
Cuts at: 3040 3407 3040
Size: 367 5343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2145 2168 2215 3031
Size: 325 217 627 337 21 23 47 816
Cuts at: 3031 4486 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 816 627 337 325 217 47
23 21
BanII G_rGCy'C
Cuts at: 1391 2386 3029 1391
Size: 995 643 4072
Fragments arranged by size:
4072 995 643
BbeI G_GCGC'C
Cuts at: 2128 2149 2128
Size: 21 5689
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2749 3602 4812 2749
Size: 853 1210 3647
Fragments arranged by size:
3647 1210 853
BclI T'GATC_A
Cuts at: 1548 2599 2643 1548
Size: 1051 44 4615
Fragments arranged by size:
4615 1051 44
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2068 2102 2143
Size: 107 122 71 631 605 396 34 41
Cuts at: 2143 4321 136
Size: 2178 1525
Fragments arranged by size:
2178 1525 631 605 396 122 107 71
41 34
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2171 2240 3771 4927 1790
Size: 381 69 1531 1156 2573
Fragments arranged by size:
2573 1531 1156 381 69
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2370 2868 4370 1239
Size: 1131 498 1502 2579
Fragments arranged by size:
2579 1502 1131 498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4373 882
Size: 617 2874 2219
Fragments arranged by size:
2874 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2125 2146 3517 3899
Size: 53 83 186 936 592 21 1371 382
Cuts at: 3899 4534 275
Size: 635 1451
Fragments arranged by size:
1451 1371 936 635 592 382 186 83
53 21
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 2765 1186
Size: 825 754 4131
Fragments arranged by size:
4131 825 754
BseYI C'CCAG_C
Cuts at: 2047 4913 5409 2047
Size: 2866 496 2348
Fragments arranged by size:
2866 2348 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3160 3921 4949 1540
Size: 275 186 1159 761 1028 2301
Fragments arranged by size:
2301 1159 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2240 2343 3771 3856 4927 2240
Size: 103 1428 85 1071 3023
Fragments arranged by size:
3023 1428 1071 103 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2240 2343 2386 3029 3771 3856
Size: 399 381 69 103 43 643 742 85
Cuts at: 3856 4927 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 643 399 381 103 85
69 43
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 2646 3599 5329 2646
Size: 953 1730 3027
Fragments arranged by size:
3027 1730 953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2274 3065 844
Size: 1308 122 791 3489
Fragments arranged by size:
3489 1308 791 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2638 3597 1394
Size: 429 815 959 3507
Fragments arranged by size:
3507 959 815 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2494 4205 4379 65
Size: 1183 1246 1711 174 1396
Fragments arranged by size:
1711 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2148 2218 2732 4354 4541 1700
Size: 110 338 70 514 1622 187 2869
Fragments arranged by size:
2869 1622 514 338 187 110 70
BsrGI T'GTAC_A
Cuts at: 96 2922 96
Size: 2826 2884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 2336 3770 4782 2336
Size: 1434 1012 3264
Fragments arranged by size:
3264 1434 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5710
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 2728 2987 1184
Size: 1544 259 3907
Fragments arranged by size:
3907 1544 259
BstYI r'GATC_y
Cuts at: 1972 2187 2946 3040 3407 3792 3809 5261 5347
Size: 215 759 94 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 759 385 367 346 215 94
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2172 2244 513
Size: 552 939 96 72 72 3979
Fragments arranged by size:
3979 939 552 96 72 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 2313 3226 4020 4040 2313
Size: 913 794 20 3983
Fragments arranged by size:
3983 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2706 2741 529
Size: 35 2142 35 3498
Fragments arranged by size:
3498 2142 35 35
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2849 4717 809
Size: 507 1533 1868 1802
Fragments arranged by size:
1868 1802 1533 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2241 3157
Size: 54 1425 50 275 18 264 147 916
Cuts at: 3157 4046 5675 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 916 889 275 264 147 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3157 1537
Size: 275 1345 4090
Fragments arranged by size:
4090 1345 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2879 3640 1353
Size: 1526 761 3423
Fragments arranged by size:
3423 1526 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2370 2547 2868 2903 3773 4370
Size: 393 730 8 177 321 35 870 597
Cuts at: 4370 5157 5425 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 730 597 393 321 268
177 35 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2062 2062
Size: 5710
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 2255 4216 2255
Size: 1961 3749
HaeII r_GCGC'y
Cuts at: 1678 2013 2128 2149 4857 1678
Size: 335 115 21 2708 2531
Fragments arranged by size:
2708 2531 335 115 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 4351 4383 4425
Size: 32 135 32 723 32 2024 32 42
Cuts at: 4425 4457 1373
Size: 32 2626
Fragments arranged by size:
2626 2024 723 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KasI G'GCGC_C
Cuts at: 2124 2145 2124
Size: 21 5689
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3309 3309
Size: 5710
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4824 5008 1305
Size: 3519 184 2007
Fragments arranged by size:
3519 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 3668 4027 4186 518
Size: 648 1077 1425 359 159 2042
Fragments arranged by size:
2042 1425 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2259 2401 3126 3803 4951 5196 1606
Size: 653 142 725 677 1148 245 2120
Fragments arranged by size:
2120 1148 725 677 653 245 142
NaeI GCC'GGC
Cuts at: 2150 2150
Size: 5710
NarI GG'CG_CC
Cuts at: 2125 2146 2125
Size: 21 5689
NcoI C'CATG_G
Cuts at: 513 1065 2004 2244 513
Size: 552 939 240 3979
Fragments arranged by size:
3979 939 552 240
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2148 2148
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 2811 3068 4613 1132
Size: 97 1582 257 1545 2229
Fragments arranged by size:
2229 1582 1545 257 97
PasI CC'CwG_GG
Cuts at: 1348 2107 1348
Size: 759 4951
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2846 2942 1108
Size: 1738 96 3876
Fragments arranged by size:
3876 1738 96
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2160 2450 3062 830
Size: 1330 290 612 3478
Fragments arranged by size:
3478 1330 612 290
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5710
PvuII CAG'CTG
Cuts at: 2401 2401
Size: 5710
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SapI GCTCTTCn'nnn_
Cuts at: 2879 2879
Size: 5710
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SfcI C'TryA_G
Cuts at: 826 1045 2156 2446 3058 4193 4874 5065 826
Size: 219 1111 290 612 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 612 290 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2102 2102
Size: 5710
SfoI GGC'GCC
Cuts at: 2126 2147 2126
Size: 21 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 2148 1700
Size: 448 5262
SmaI CCC'GGG
Cuts at: 1872 3037 1872
Size: 1165 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 2811 3068 1132
Size: 1679 257 3774
Fragments arranged by size:
3774 1679 257
SspI AAT'ATT
Cuts at: 5 52 2722 3634 5
Size: 47 2670 912 2081
Fragments arranged by size:
2670 2081 912 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 3149 513
Size: 552 939 240 905 3074
Fragments arranged by size:
3074 939 905 552 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3737 3754 3907 4092 4537 325
Size: 1255 2157 17 153 185 445 1498
Fragments arranged by size:
2157 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2922 3956 5668
Size: 274 80 33 51 1443 945 1034 1712
Cuts at: 5668 96
Size: 138
Fragments arranged by size:
1712 1443 1034 945 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5670 5702 5670
Size: 32 5678
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2055 2055
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 3035 1870
Size: 1165 4545
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SrfI SwaI