Restriction map for
pFN21AE0465
(Circular) (Six-base) MAPSORT of: pFN21AE0465.seq Check: 1413 from: 1 to: 5362
pFN21AE0465 5362 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2705 1937
Size: 768 4594
Acc65I G'GTAC_C
Cuts at: 2683 2683
Size: 5362
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3425 4809 5077 1632
Size: 1793 1384 268 1917
Fragments arranged by size:
1917 1793 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 4261 5238 1203
Size: 3058 977 1327
Fragments arranged by size:
3058 1327 977
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 4091 4091
Size: 5362
AleI CACnn'nnGTG
Cuts at: 1166 2274 1166
Size: 1108 4254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5362
AlwNI CAG_nnn'CTG
Cuts at: 2366 3105 4677 2366
Size: 739 1572 3051
Fragments arranged by size:
3051 1572 739
ApaI G_GGCC'C
Cuts at: 2461 2461
Size: 5362
ApaLI G'TGCA_C
Cuts at: 3419 4575 3419
Size: 1156 4206
ApoI r'AATT_y
Cuts at: 1493 2140 2671 2897 3049 5158 1493
Size: 647 531 226 152 2109 1697
Fragments arranged by size:
2109 1697 647 531 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 1870
Size: 73 744 4545
Fragments arranged by size:
4545 744 73
AvrII C'CTAG_G
Cuts at: 2321 2321
Size: 5362
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2210 2243 2210
Size: 33 5329
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 2518 2548 2683 4138
Size: 325 217 627 701 30 30 135 1455
Cuts at: 4138 618
Size: 1842
Fragments arranged by size:
1842 1455 701 627 325 217 135 30
30
BanII G_rGCy'C
Cuts at: 1391 2461 2681 1391
Size: 1070 220 4072
Fragments arranged by size:
4072 1070 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3553 3587 1903
Size: 34 1616 34 3678
Fragments arranged by size:
3678 1616 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2238 3254 4464 2238
Size: 1016 1210 3136
Fragments arranged by size:
3136 1210 1016
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3973 136
Size: 107 122 71 631 605 2301 1525
Fragments arranged by size:
2301 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5357 5357
Size: 5362
BlpI GC'TnA_GC
Cuts at: 2397 2442 2472 2502 2532 2592 2779 2397
Size: 45 30 30 30 60 187 4980
Fragments arranged by size:
4980 187 60 45 30 30 30
Bme1580I G_kGCm'C
Cuts at: 1790 2461 3423 4579 1790
Size: 671 962 1156 2573
Fragments arranged by size:
2573 1156 962 671
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2162 2178 3093
Size: 416 53 83 399 24 745 16 915
Cuts at: 3093 4051 442
Size: 958 1753
Fragments arranged by size:
1753 958 915 745 416 399 83 53
24 16
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4022 1239
Size: 2783 2579
Bpu10I CC'TnA_GC
Cuts at: 2287 2287
Size: 5362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3487 4352 4650 3487
Size: 865 298 4199
Fragments arranged by size:
4199 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4025 882
Size: 617 2526 2219
Fragments arranged by size:
2526 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3153 493
Size: 711 1949 2702
Fragments arranged by size:
2702 1949 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3169 3551 4186 275
Size: 53 83 186 936 1636 382 635 1451
Fragments arranged by size:
1636 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3795 4193 4467 4614 5025 3795
Size: 398 274 147 411 4132
Fragments arranged by size:
4132 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5137
Fragments arranged by size:
5137 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2142 2301 1186
Size: 956 159 4247
Fragments arranged by size:
4247 956 159
BseYI C'CCAG_C
Cuts at: 4565 5061 4565
Size: 496 4866
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2397 1163
Size: 573 661 4128
Fragments arranged by size:
4128 661 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2812 3573 4601 1540
Size: 275 186 811 761 1028 2301
Fragments arranged by size:
2301 1028 811 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3423 3508 4579 3423
Size: 85 1071 4206
Fragments arranged by size:
4206 1071 85
BsmI GAATG_Cn'
Cuts at: 2873 2966 2873
Size: 93 5269
BsmBI CGTCTCn'nnnn_
Cuts at: 2050 2050
Size: 5362
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2461 2681 3423 3508 4579 1391
Size: 399 671 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 671 399 220 85
BspEI T'CCGG_A
Cuts at: 5025 5025
Size: 5362
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2717 844
Size: 1873 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3249 1394
Size: 429 1426 3507
Fragments arranged by size:
3507 1426 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3857 4031 65
Size: 1183 2609 174 1396
Fragments arranged by size:
2609 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4006 4193 1700
Size: 110 2196 187 2869
Fragments arranged by size:
2869 2196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 3422 4434 3422
Size: 1012 4350
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2378 2525 794
Size: 1584 147 3631
Fragments arranged by size:
3631 1584 147
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 2096 2217 1184
Size: 912 121 4329
Fragments arranged by size:
4329 912 121
BstYI r'GATC_y
Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357
Size: 720 367 385 17 1452 86 12 346
Cuts at: 5357 1972
Size: 1977
Fragments arranged by size:
1977 1452 720 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2275 513
Size: 552 1210 3600
Fragments arranged by size:
3600 1210 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4623
Fragments arranged by size:
4623 683 56
BtsI GCAGTG_nn'
Cuts at: 2878 3672 3692 2878
Size: 794 20 4548
Fragments arranged by size:
4548 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5327
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4369 809
Size: 507 3053 1802
Fragments arranged by size:
3053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2809 3698 5327
Size: 54 1425 50 275 18 979 889 1629
Cuts at: 5327 8
Size: 43
Fragments arranged by size:
1629 1425 979 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2809 1537
Size: 275 997 4090
Fragments arranged by size:
4090 997 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2048 3292 1353
Size: 695 1244 3423
Fragments arranged by size:
3423 1244 695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3949 4323 4469 387
Size: 1064 138 2360 374 146 1280
Fragments arranged by size:
2360 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3425 4022 4809 5077 1239
Size: 393 1793 597 787 268 1524
Fragments arranged by size:
1793 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2451 2451
Size: 5362
EcoO109I rG'GnC_Cy
Cuts at: 2457 2458 2457
Size: 1 5361
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FspI TGC'GCA
Cuts at: 3868 3868
Size: 5362
HaeII r_GCGC'y
Cuts at: 1678 4509 1678
Size: 2831 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4003 4035 4077 4109 1373
Size: 32 135 32 2431 32 42 32 2626
Fragments arranged by size:
2626 2431 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2706 2952 669
Size: 988 281 768 246 3079
Fragments arranged by size:
3079 988 768 281 246
HindIII A'AGCT_T
Cuts at: 748 2009 748
Size: 1261 4101
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2687 2687
Size: 5362
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2476 2536 4476 4660 1305
Size: 1171 60 1940 184 2007
Fragments arranged by size:
2007 1940 1171 184 60
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3540
Fragments arranged by size:
3540 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 3320 3679 3838 518
Size: 648 1108 1046 359 159 2042
Fragments arranged by size:
2042 1108 1046 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2366 2378 2396 2411 2441 2471 2531 2570
Size: 760 12 18 15 30 30 60 39
Cuts at: 2570 2591 2778 3455 4603 4848 1606
Size: 21 187 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 245 187 60 39
30 30 21 18 15 12
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4810
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5362
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2720 4265 1132
Size: 97 1491 1545 2229
Fragments arranged by size:
2229 1545 1491 97
PasI CC'CwG_GG
Cuts at: 1348 2192 1348
Size: 844 4518
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5362
PfoI T'CCnGG_A
Cuts at: 2248 2248
Size: 5362
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2094 1613
Size: 481 4881
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PspOMI G'GGCC_C
Cuts at: 2457 2457
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2371 2410 2455 2485 2515 2545 2575 2714
Size: 1541 39 45 30 30 30 30 139
Cuts at: 2714 830
Size: 3478
Fragments arranged by size:
3478 1541 139 45 39 30 30 30
30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2366 2396 2411 2441 2471 2531 2570 2591 2366
Size: 30 15 30 30 60 39 21 5137
Fragments arranged by size:
5137 60 39 30 30 30 21 15
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 3610 5322 3610
Size: 1712 3650
SfcI C'TryA_G
Cuts at: 826 1045 2367 2406 2433 2451 2481 2511 2541
Size: 219 1322 39 27 18 30 30 30
Cuts at: 2541 2571 2601 2710 3845 4526 4717 826
Size: 30 30 109 1135 681 191 1471
Fragments arranged by size:
1471 1322 1135 681 219 191 109 39
30 30 30 30 30 27 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2689 1872
Size: 817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3466 4367 4629 820
Size: 197 926 1523 901 262 1553
Fragments arranged by size:
1553 1523 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 2122 3286 5
Size: 47 2070 1164 2081
Fragments arranged by size:
2081 2070 1164 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5362
StyI C'CwwG_G
Cuts at: 513 1065 2321 2801 513
Size: 552 1256 480 3074
Fragments arranged by size:
3074 1256 552 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 3389 3406 3559 3744 4189 325
Size: 1255 535 1274 17 153 185 445 1498
Fragments arranged by size:
1498 1274 1255 535 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3608 5320 96
Size: 274 80 33 51 1443 1631 1712 138
Fragments arranged by size:
1712 1631 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5322 5354 5322
Size: 32 5330
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5362
XbaI T'CTAG_A
Cuts at: 2698 2698
Size: 5362
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5362
XmaI C'CCGG_G
Cuts at: 1870 2687 1870
Size: 817 4545
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI