Restriction map for
pFN21AE0461
(Circular) (Six-base) MAPSORT of: pFN21AE0461.seq Check: 8967 from: 1 to: 5350
pFN21AE0461 5350 bp
With 182 enzymes: SgfI *
October 20, 2009 10:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3160 278
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 1937
Size: 756 4594
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2454 2571 3413 4797 5065 1632
Size: 822 117 842 1384 268 1917
Fragments arranged by size:
1917 1384 842 822 268 117
AflII C'TTAA_G
Cuts at: 820 1017 2308 820
Size: 197 1291 3862
Fragments arranged by size:
3862 1291 197
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5350
AlwNI CAG_nnn'CTG
Cuts at: 3093 4665 3093
Size: 1572 3778
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2108 2144 2659 2885 3037 5146 1493
Size: 615 36 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 615 515 226 152 36
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 1870
Size: 73 732 4545
Fragments arranged by size:
4545 732 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 4126 618
Size: 325 217 627 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2669 1391
Size: 875 403 4072
Fragments arranged by size:
4072 875 403
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2447 2481 3541 3575 1903
Size: 34 510 34 1060 34 3678
Fragments arranged by size:
3678 1060 510 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2194 3242 4452 2194
Size: 1048 1210 3092
Fragments arranged by size:
3092 1210 1048
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3961 136
Size: 107 122 71 631 605 2289 1525
Fragments arranged by size:
2289 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5345 5345
Size: 5350
BlpI GC'TnA_GC
Cuts at: 2767 2767
Size: 5350
Bme1580I G_kGCm'C
Cuts at: 1790 3411 4567 1790
Size: 1621 1156 2573
Fragments arranged by size:
2573 1621 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3081 4039 442
Size: 416 53 83 399 24 1664 958 1753
Fragments arranged by size:
1753 1664 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4010 1239
Size: 2771 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 3475 4340 4638 2522
Size: 953 865 298 3234
Fragments arranged by size:
3234 953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4013 882
Size: 617 2514 2219
Fragments arranged by size:
2514 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 5344 5344
Size: 5350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3157 3539 4174 275
Size: 53 83 186 936 1624 382 635 1451
Fragments arranged by size:
1624 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3783 4181 4455 4602 5013 3783
Size: 398 274 147 411 4120
Fragments arranged by size:
4120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2469 2499 1178
Size: 30 165 30 1066 30 4029
Fragments arranged by size:
4029 1066 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 1186
Size: 1079 4271
BseYI C'CCAG_C
Cuts at: 4553 5049 4553
Size: 496 4854
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1998 1163
Size: 573 262 4515
Fragments arranged by size:
4515 573 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2266 3411 3496 4567 2266
Size: 1145 85 1071 3049
Fragments arranged by size:
3049 1145 1071 85
BsmI GAATG_Cn'
Cuts at: 2560 2861 2954 2560
Size: 301 93 4956
Fragments arranged by size:
4956 301 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2669 3411 3496 4567 1391
Size: 399 476 403 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 476 403 399 85
BspEI T'CCGG_A
Cuts at: 5013 5013
Size: 5350
BspHI T'CATG_A
Cuts at: 3239 4969 3239
Size: 1730 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 844
Size: 1861 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 1394
Size: 429 1414 3507
Fragments arranged by size:
3507 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2604 3994 4181 1700
Size: 110 794 1390 187 2869
Fragments arranged by size:
2869 1390 794 187 110
BsrGI T'GTAC_A
Cuts at: 96 2572 96
Size: 2476 2874
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 3410 4422 3410
Size: 1012 4338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5350
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstYI r'GATC_y
Cuts at: 1972 2548 2680 3047 3432 3449 4901 4987 4999
Size: 576 132 367 385 17 1452 86 12
Cuts at: 4999 5345 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 576 385 367 346 132 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3859
Fragments arranged by size:
3859 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2041 2654 2041
Size: 613 4737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 2797 3686
Size: 54 1425 50 275 18 343 624 889
Cuts at: 3686 5315 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 624 343 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3280 1353
Size: 1927 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3937 4311 4457 387
Size: 1064 138 2348 374 146 1280
Fragments arranged by size:
2348 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2264 2264
Size: 5350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2454 2571 3413 4010 4797 5065 1239
Size: 393 822 117 842 597 787 268 1524
Fragments arranged by size:
1524 842 822 787 597 393 268 117
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
EcoRV GAT'ATC
Cuts at: 2281 2281
Size: 5350
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 4497 1678
Size: 2819 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3991 4023 4065 4097 1373
Size: 32 135 32 2419 32 42 32 2626
Fragments arranged by size:
2626 2419 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2322 2694 2940 669
Size: 988 281 384 372 246 3079
Fragments arranged by size:
3079 988 384 372 281 246
HindIII A'AGCT_T
Cuts at: 748 2542 748
Size: 1794 3556
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2133 2949 2133
Size: 816 4534
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4464 4648 1305
Size: 3159 184 2007
Fragments arranged by size:
3159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2175 10
Size: 54 1425 343 343 3185
Fragments arranged by size:
3185 1425 343 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3308 3667 3826 518
Size: 648 2142 359 159 2042
Fragments arranged by size:
2142 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2316 2766 3443 4591 4836 1606
Size: 710 450 677 1148 245 2120
Fragments arranged by size:
2120 1148 710 677 450 245
NaeI GCC'GGC
Cuts at: 2606 2606
Size: 5350
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3859
Fragments arranged by size:
3859 939 552
NdeI CA'TA_TG
Cuts at: 387 2410 387
Size: 2023 3327
NgoMIV G'CCGG_C
Cuts at: 2604 2604
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5350
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4932 4964 3419
Size: 32 1481 32 3805
Fragments arranged by size:
3805 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2193 2702 830
Size: 1363 509 3478
Fragments arranged by size:
3478 1363 509
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
PvuII CAG'CTG
Cuts at: 2316 2316
Size: 5350
SacI G_AGCT'C
Cuts at: 2266 2266
Size: 5350
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2189 2245 2698 3833 4514 4705 826
Size: 219 1144 56 453 1135 681 191 1471
Fragments arranged by size:
1471 1144 1135 681 453 219 191 56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2308 2501 3454 4355 4617 820
Size: 197 926 365 193 953 901 262 1553
Fragments arranged by size:
1553 953 926 901 365 262 197 193
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2004 2789 513
Size: 552 939 785 3074
Fragments arranged by size:
3074 939 785 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3377 3394 3547 3732 4177 325
Size: 1255 1797 17 153 185 445 1498
Fragments arranged by size:
1797 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2294 2572 3596
Size: 274 80 33 51 1443 317 278 1024
Cuts at: 3596 5308 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1024 317 278 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 3479 3479
Size: 5350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3158 276
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI