Restriction map for
pFN21AE0453
(Circular) (Six-base) MAPSORT of: pFN21AE0453.seq Check: 5409 from: 1 to: 5386
pFN21AE0453 5386 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2014 2707 2014
Size: 693 4693
AclI AA'CG_TT
Cuts at: 1563 2685 3513 3886 1563
Size: 1122 828 373 3063
Fragments arranged by size:
3063 1122 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4833 5101 1632
Size: 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AlwNI CAG_nnn'CTG
Cuts at: 3129 4701 3129
Size: 1572 3814
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2213 2458 2695 2921 3073 5182 1493
Size: 720 245 237 226 152 2109 1697
Fragments arranged by size:
2109 1697 720 245 237 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2711 1870
Size: 73 103 665 4545
Fragments arranged by size:
4545 665 103 73
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2707 4162 618
Size: 325 217 627 227 693 1455 1842
Fragments arranged by size:
1842 1455 693 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2160 2705 1391
Size: 769 545 4072
Fragments arranged by size:
4072 769 545
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BbvCI CC'TCA_GC
Cuts at: 2652 2652
Size: 5386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2487 3278 4488 2487
Size: 791 1210 3385
Fragments arranged by size:
3385 1210 791
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 136
Size: 107 122 71 631 605 2325 1525
Fragments arranged by size:
2325 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 3447 4603 1790
Size: 1657 1156 2573
Fragments arranged by size:
2573 1657 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 2428 3117
Size: 416 53 83 399 24 982 29 689
Cuts at: 3117 4075 442
Size: 958 1753
Fragments arranged by size:
1753 982 958 689 416 399 83 53
29 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 4046 1239
Size: 884 1923 2579
Fragments arranged by size:
2579 1923 884
Bpu10I CC'TnA_GC
Cuts at: 2652 2652
Size: 5386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 4376 4674 3511
Size: 865 298 4223
Fragments arranged by size:
4223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2446 4049 882
Size: 617 947 1603 2219
Fragments arranged by size:
2219 1603 947 617
BsaAI yAC'GTr
Cuts at: 493 1204 3177 493
Size: 711 1973 2702
Fragments arranged by size:
2702 1973 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3193 3575 4210 275
Size: 53 83 186 936 1660 382 635 1451
Fragments arranged by size:
1660 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3819 4217 4491 4638 5049 3819
Size: 398 274 147 411 4156
Fragments arranged by size:
4156 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5161
Fragments arranged by size:
5161 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2248 2460 1186
Size: 1062 212 4112
Fragments arranged by size:
4112 1062 212
BseYI C'CCAG_C
Cuts at: 2299 4589 5085 2299
Size: 2290 496 2600
Fragments arranged by size:
2600 2290 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2475 2574 1163
Size: 573 739 99 3975
Fragments arranged by size:
3975 739 573 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3597 4625 1540
Size: 275 186 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2271 3447 3532 4603 2271
Size: 1176 85 1071 3054
Fragments arranged by size:
3054 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2271 2705 3447 3532 4603 1391
Size: 399 370 111 434 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 434 399 370 111 85
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 3275 5005 3275
Size: 1730 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 2741 844
Size: 1811 86 3489
Fragments arranged by size:
3489 1811 86
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 1394
Size: 429 1450 3507
Fragments arranged by size:
3507 1450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3881 4055 65
Size: 1183 2633 174 1396
Fragments arranged by size:
2633 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2036 2271 4030 4217 1700
Size: 110 226 235 1759 187 2869
Fragments arranged by size:
2869 1759 235 226 187 110
BsrGI T'GTAC_A
Cuts at: 96 2260 96
Size: 2164 3222
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5386
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381
Size: 744 367 385 17 1452 86 12 346
Cuts at: 5381 1972
Size: 1977
Fragments arranged by size:
1977 1452 744 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2508 2508
Size: 5386
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4834
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4647
Fragments arranged by size:
4647 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2833 3722 5351
Size: 54 1425 50 275 18 1003 889 1629
Cuts at: 5351 8
Size: 43
Fragments arranged by size:
1629 1425 1003 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3316 1353
Size: 1963 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 4347 4493 387
Size: 1064 138 2384 374 146 1280
Fragments arranged by size:
2384 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 3449 4046 4833 5101 1239
Size: 393 491 1326 597 787 268 1524
Fragments arranged by size:
1524 1326 787 597 491 393 268
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 4533 1678
Size: 2855 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 4027 4059 4101
Size: 32 135 32 805 32 1618 32 42
Cuts at: 4101 4133 1373
Size: 32 2626
Fragments arranged by size:
2626 1618 805 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KpnI G_GTAC'C
Cuts at: 2018 2711 2018
Size: 693 4693
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4500 4684 1305
Size: 3195 184 2007
Fragments arranged by size:
3195 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3344 3703 3862 518
Size: 648 843 1335 359 159 2042
Fragments arranged by size:
2042 1335 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2299 2802 3479 4627 4872 1606
Size: 693 503 677 1148 245 2120
Fragments arranged by size:
2120 1148 693 677 503 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4834
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2744 4289 1132
Size: 97 1515 1545 2229
Fragments arranged by size:
2229 1545 1515 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2627 1108
Size: 1519 3867
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2235 2267 2235
Size: 32 5354
PstI C_TGCA'G
Cuts at: 830 2298 2738 830
Size: 1468 440 3478
Fragments arranged by size:
3478 1468 440
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
PvuII CAG'CTG
Cuts at: 2299 2299
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SexAI A'CCwGG_T
Cuts at: 2323 2323
Size: 5386
SfcI C'TryA_G
Cuts at: 826 1045 2241 2294 2734 3869 4550 4741 826
Size: 219 1196 53 440 1135 681 191 1471
Fragments arranged by size:
1471 1196 1135 681 440 219 191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2048 2713 1872
Size: 176 665 4545
Fragments arranged by size:
4545 665 176
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3490 4391 4653 820
Size: 197 926 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2744 1132
Size: 1612 3774
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2825 513
Size: 552 1760 3074
Fragments arranged by size:
3074 1760 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3413 3430 3583 3768 4213 325
Size: 1255 1833 17 153 185 445 1498
Fragments arranged by size:
1833 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 2260 3632
Size: 274 80 33 51 1443 96 187 1372
Cuts at: 3632 5344 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1372 274 187 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5346 5378 5346
Size: 32 5354
Tth111I GACn'n_nGTC
Cuts at: 1316 2420 1316
Size: 1104 4282
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2046 2711 1870
Size: 176 665 4545
Fragments arranged by size:
4545 665 176
XmnI GAAnn'nnTTC
Cuts at: 2250 3515 2250
Size: 1265 4121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI