Restriction map for
pFN21AE0447
(Circular) (Six-base) MAPSORT of: pFN21AE0447.seq Check: 6514 from: 1 to: 5230
pFN21AE0447 5230 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2126 3040 278
Size: 53 83 186 936 590 914 2468
Fragments arranged by size:
2468 936 914 590 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2126 2388 2573 1937
Size: 189 262 185 4594
Fragments arranged by size:
4594 262 189 185
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3293 4677 4945 1632
Size: 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2973 4545 2973
Size: 1572 3658
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2031 2373 2560 2927 2031
Size: 342 187 367 4334
Fragments arranged by size:
4334 367 342 187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2551 4006 618
Size: 325 217 627 386 378 1455 1842
Fragments arranged by size:
1842 1455 627 386 378 325 217
BanII G_rGCy'C
Cuts at: 1391 2549 1391
Size: 1158 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 2176 3291 4447 1790
Size: 386 1115 1156 2573
Fragments arranged by size:
2573 1156 1115 386
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2332 3890 1239
Size: 1093 1558 2579
Fragments arranged by size:
2579 1558 1093
Bpu10I CC'TnA_GC
Cuts at: 2469 2469
Size: 5230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2464 3893 882
Size: 617 965 1429 2219
Fragments arranged by size:
2219 1429 965 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3037 3419 4054
Size: 53 83 186 936 590 914 382 635
Cuts at: 4054 275
Size: 1451
Fragments arranged by size:
1451 936 914 635 590 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2370 3663 4061 4335 4482 4893 2370
Size: 1293 398 274 147 411 2707
Fragments arranged by size:
2707 1293 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 2063 2177 2377 4433 4929 2063
Size: 114 200 2056 496 2364
Fragments arranged by size:
2364 2056 496 200 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 2097 3291 3376 4447 2097
Size: 1194 85 1071 2880
Fragments arranged by size:
2880 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2176 2549 3291 3376 4447 1391
Size: 399 307 79 373 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 373 307 85 79
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 844
Size: 1741 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 1394
Size: 429 1294 3507
Fragments arranged by size:
3507 1294 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2031 2373 2560 2927 3312 3329 4781 4867
Size: 59 342 187 367 385 17 1452 86
Cuts at: 4867 4879 5225 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 342 187 86
59 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 1996 487
Size: 683 56 770 3721
Fragments arranged by size:
3721 770 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2153 2677 3566
Size: 54 1425 50 275 18 323 524 889
Cuts at: 3566 5195 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 524 323 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2490 3160 1353
Size: 960 177 670 3423
Fragments arranged by size:
3423 960 670 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3817 4191 4337 387
Size: 1064 138 435 1793 374 146 1280
Fragments arranged by size:
1793 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2332 3293 3890 4677 4945 1239
Size: 393 700 961 597 787 268 1524
Fragments arranged by size:
1524 961 787 700 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 2216
Size: 5230
EcoO109I rG'GnC_Cy
Cuts at: 2428 2428
Size: 5230
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 2326 4377 1678
Size: 648 2051 2531
Fragments arranged by size:
2531 2051 648
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 3871 3903 3945
Size: 32 135 32 641 32 1626 32 42
Cuts at: 3945 3977 1373
Size: 32 2626
Fragments arranged by size:
2626 1626 641 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 2574 2820 669
Size: 988 281 298 338 246 3079
Fragments arranged by size:
3079 988 338 298 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 4344 4528 1305
Size: 777 2262 184 2007
Fragments arranged by size:
2262 2007 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2181 2381 2646 3323 4471 4716 1606
Size: 575 200 265 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 575 265 245 200
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2588 4133 1132
Size: 97 1359 1545 2229
Fragments arranged by size:
2229 1545 1359 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 2428
Size: 5230
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
PvuII CAG'CTG
Cuts at: 2181 2381 2181
Size: 200 5030
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SapI GCTCTTCn'nnn_
Cuts at: 2313 2313
Size: 5230
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2447 2669 513
Size: 552 939 443 222 3074
Fragments arranged by size:
3074 939 552 443 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 3257 3274 3427 3612 4057 325
Size: 1255 611 1066 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1066 611 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3476 5188 96
Size: 274 80 33 51 1443 1499 1712 138
Fragments arranged by size:
1712 1499 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2148 2180 5190 5222 2148
Size: 32 3010 32 2156
Fragments arranged by size:
3010 2156 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2333
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 2252 3359 2252
Size: 1107 4123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2124 3038 276
Size: 53 83 186 936 590 914 2468
Fragments arranged by size:
2468 936 914 590 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI