Restriction map for
pFN21AE0437
(Circular) (Six-base) MAPSORT of: pFN21AE0437.seq Check: 7426 from: 1 to: 5635
pFN21AE0437 5635 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3445 278
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2010 2978 1937
Size: 73 968 4594
Fragments arranged by size:
4594 968 73
Acc65I G'GTAC_C
Cuts at: 2956 2956
Size: 5635
AclI AA'CG_TT
Cuts at: 1563 3762 4135 1563
Size: 2199 373 3063
Fragments arranged by size:
3063 2199 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 3698 5082 5350 1632
Size: 783 1283 1384 268 1917
Fragments arranged by size:
1917 1384 1283 783 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5438
AflIII A'CryG_T
Cuts at: 1203 4534 5511 1203
Size: 3331 977 1327
Fragments arranged by size:
3331 1327 977
AgeI A'CCGG_T
Cuts at: 4466 4466
Size: 5635
AhdI GACnn_n'nnGTC
Cuts at: 2893 4364 2893
Size: 1471 4164
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2445 1184
Size: 1261 4374
AlwNI CAG_nnn'CTG
Cuts at: 2065 2162 3378 4950 2065
Size: 97 1216 1572 2750
Fragments arranged by size:
2750 1572 1216 97
ApaLI G'TGCA_C
Cuts at: 3692 4848 3692
Size: 1156 4479
ApoI r'AATT_y
Cuts at: 1493 2944 3170 3322 5431 1493
Size: 1451 226 152 2109 1697
Fragments arranged by size:
2109 1697 1451 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4189 160
Size: 872 3157 1606
Fragments arranged by size:
3157 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
AvaI C'yCGr_G
Cuts at: 1870 1943 2383 2960 1870
Size: 73 440 577 4545
Fragments arranged by size:
4545 577 440 73
BamHI G'GATC_C
Cuts at: 2965 3332 2965
Size: 367 5268
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2956 4411 618
Size: 325 217 627 1169 1455 1842
Fragments arranged by size:
1842 1455 1169 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2572 2703 2954 1391
Size: 1181 131 251 4072
Fragments arranged by size:
4072 1181 251 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2875 928
Size: 971 976 3688
Fragments arranged by size:
3688 976 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3826 3860 1903
Size: 34 1889 34 3678
Fragments arranged by size:
3678 1889 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3527 4737 3527
Size: 1210 4425
BclI T'GATC_A
Cuts at: 1548 2739 1548
Size: 1191 4444
BfrBI ATG'CAT
Cuts at: 2762 2762
Size: 5635
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4246 136
Size: 107 122 71 631 605 2574 1525
Fragments arranged by size:
2574 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5630 5630
Size: 5635
BlpI GC'TnA_GC
Cuts at: 2433 2640 3052 2433
Size: 207 412 5016
Fragments arranged by size:
5016 412 207
Bme1580I G_kGCm'C
Cuts at: 1790 2390 3696 4852 1790
Size: 600 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 600
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5635
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2198 3366 4324
Size: 416 53 83 399 24 781 1168 958
Cuts at: 4324 442
Size: 1753
Fragments arranged by size:
1753 1168 958 781 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5635
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 5635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2448 2586 4295 1239
Size: 1209 138 1709 2579
Fragments arranged by size:
2579 1709 1209 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2442 3760 4625 4923 2442
Size: 1318 865 298 3154
Fragments arranged by size:
3154 1318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2708 4298 882
Size: 617 1209 1590 2219
Fragments arranged by size:
2219 1590 1209 617
BsaAI yAC'GTr
Cuts at: 493 1204 3426 493
Size: 711 2222 2702
Fragments arranged by size:
2702 2222 711
BsaBI GATnn'nnATC
Cuts at: 5629 5629
Size: 5635
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3442 3824 4459 275
Size: 53 83 186 936 1909 382 635 1451
Fragments arranged by size:
1909 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4068 4466 4740 4887 5298 4068
Size: 398 274 147 411 4405
Fragments arranged by size:
4405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 2122 2150 2152
Size: 30 165 30 659 30 30 28 2
Cuts at: 2152 2180 2522 2552 2554 2584 2915 2945 1178
Size: 28 342 30 2 30 331 30 3868
Fragments arranged by size:
3868 659 342 331 165 30 30 30
30 30 30 30 28 28 2 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2612 1186
Size: 1096 330 4209
Fragments arranged by size:
4209 1096 330
BseYI C'CCAG_C
Cuts at: 4838 5334 4838
Size: 496 5139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2562 1163
Size: 573 826 4236
Fragments arranged by size:
4236 826 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3085 3846 4874 1540
Size: 275 186 1084 761 1028 2301
Fragments arranged by size:
2301 1084 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2572 2703 3696 3781 4852 2202
Size: 370 131 993 85 1071 2985
Fragments arranged by size:
2985 1071 993 370 131 85
BsmI GAATG_Cn'
Cuts at: 2854 3146 3239 2854
Size: 292 93 5250
Fragments arranged by size:
5250 292 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2390 2572 2703 2954 3696 3781
Size: 399 412 188 182 131 251 742 85
Cuts at: 3781 4852 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 412 399 251 188 182
131 85
BspEI T'CCGG_A
Cuts at: 5298 5298
Size: 5635
BspHI T'CATG_A
Cuts at: 3524 5254 3524
Size: 1730 3905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2990 844
Size: 2146 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3522 1394
Size: 429 1699 3507
Fragments arranged by size:
3507 1699 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2802 4130 4304 65
Size: 1183 1554 1328 174 1396
Fragments arranged by size:
1554 1396 1328 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4279 4466 1700
Size: 110 2469 187 2869
Fragments arranged by size:
2869 2469 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5635
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5635
BssSI C'ACGA_G
Cuts at: 3695 4707 3695
Size: 1012 4623
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2162 794
Size: 1368 4267
BstBI TT'CG_AA
Cuts at: 2288 4439 2288
Size: 2151 3484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5635
BstXI CCAn_nnnn'nTGG
Cuts at: 2564 2564
Size: 5635
BstYI r'GATC_y
Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630
Size: 993 367 385 17 1452 86 12 346
Cuts at: 5630 1972
Size: 1977
Fragments arranged by size:
1977 1452 993 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2502 2502
Size: 5635
BtgI C'CryG_G
Cuts at: 513 1065 2004 2808 513
Size: 552 939 804 3340
Fragments arranged by size:
3340 939 804 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2764 487
Size: 683 56 1538 3358
Fragments arranged by size:
3358 1538 683 56
BtsI GCAGTG_nn'
Cuts at: 2160 3151 3945 3965 2160
Size: 991 794 20 3830
Fragments arranged by size:
3830 991 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5635
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5600
DraI TTT'AAA
Cuts at: 2939 2939
Size: 5635
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2599 4642 809
Size: 507 1283 2043 1802
Fragments arranged by size:
2043 1802 1283 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3082 3971 5600
Size: 54 1425 50 275 18 1252 889 1629
Cuts at: 5600 8
Size: 43
Fragments arranged by size:
1629 1425 1252 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3082 1537
Size: 275 1270 4090
Fragments arranged by size:
4090 1270 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2100 2259 3565 1353
Size: 674 73 159 1306 3423
Fragments arranged by size:
3423 1306 674 159 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4222 4596 4742 387
Size: 1064 138 2633 374 146 1280
Fragments arranged by size:
2633 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2570 2701 2570
Size: 131 5504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2448 2586 3698 4295 5082 5350
Size: 393 783 33 138 1112 597 787 268
Cuts at: 5350 1239
Size: 1524
Fragments arranged by size:
1524 1112 787 783 597 393 268 138
33
EcoNI CCTnn'n_nnAGG
Cuts at: 2397 2731 2397
Size: 334 5301
EcoO109I rG'GnC_Cy
Cuts at: 2895 2895
Size: 5635
EcoRI G'AATT_C
Cuts at: 2944 3322 2944
Size: 378 5257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 2038
Size: 5635
FspI TGC'GCA
Cuts at: 4141 4141
Size: 5635
HaeII r_GCGC'y
Cuts at: 1678 4782 1678
Size: 3104 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2122 2150 2154 2182 2879
Size: 32 135 32 550 28 4 28 697
Cuts at: 2879 2911 4276 4308 4350 4382 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1365 697 550 135 42 32 32
32 32 32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2337 2979 3225 669
Size: 988 281 399 642 246 3079
Fragments arranged by size:
3079 988 642 399 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5635
HpaI GTT'AAC
Cuts at: 3225 3225
Size: 5635
KpnI G_GTAC'C
Cuts at: 2960 2960
Size: 5635
MfeI C'AATT_G
Cuts at: 3234 3234
Size: 5635
MluI A'CGCG_T
Cuts at: 5511 5511
Size: 5635
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2123 2199 2597 4749 4933 1305
Size: 818 76 398 2152 184 2007
Fragments arranged by size:
2152 2007 818 398 184 76
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3813
Fragments arranged by size:
3813 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2708 2792 2807 3593 3952 4111 518
Size: 648 1542 84 15 786 359 159 2042
Fragments arranged by size:
2042 1542 786 648 359 159 84 15
MspA1I CmG'CkG
Cuts at: 1606 2429 2639 3051 3728 4876 5121 1606
Size: 823 210 412 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 677 412 245 210
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4144
Fragments arranged by size:
4144 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5635
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5635
NotI GC'GGCC_GC
Cuts at: 3082 3082
Size: 5635
NsiI A_TGCA'T
Cuts at: 2764 2764
Size: 5635
NspI r_CATG'y
Cuts at: 1132 1229 2633 2762 2993 4538 1132
Size: 97 1404 129 231 1545 2229
Fragments arranged by size:
2229 1545 1404 231 129 97
PasI CC'CwG_GG
Cuts at: 1348 2733 1348
Size: 1385 4250
PciI A'CATG_T
Cuts at: 4534 4534
Size: 5635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2127 1108
Size: 1019 4616
PmeI GTTT'AAAC
Cuts at: 2939 2939
Size: 5635
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 5217 5249 3704
Size: 32 1481 32 4090
Fragments arranged by size:
4090 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2895 2895
Size: 5635
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5635
PsiI TTA'TAA
Cuts at: 3205 3205
Size: 5635
PstI C_TGCA'G
Cuts at: 830 2987 830
Size: 2157 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5635
PvuII CAG'CTG
Cuts at: 2639 2639
Size: 5635
SacI G_AGCT'C
Cuts at: 2572 2703 2572
Size: 131 5504
SalI G'TCGA_C
Cuts at: 1936 2977 1936
Size: 1041 4594
SbfI CC_TGCA'GG
Cuts at: 2987 2987
Size: 5635
ScaI AGT'ACT
Cuts at: 3883 5595 3883
Size: 1712 3923
SfcI C'TryA_G
Cuts at: 826 1045 2983 4118 4799 4990 826
Size: 219 1938 1135 681 191 1471
Fragments arranged by size:
1938 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5635
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5635
SmaI CCC'GGG
Cuts at: 1872 2962 1872
Size: 1090 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2457 3739 4640 4902 820
Size: 197 926 514 1282 901 262 1553
Fragments arranged by size:
1553 1282 926 901 514 262 197
SnaBI TAC'GTA
Cuts at: 493 3426 493
Size: 2933 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5635
SphI G_CATG'C
Cuts at: 1132 2993 1132
Size: 1861 3774
SspI AAT'ATT
Cuts at: 5 52 3559 5
Size: 47 3507 2081
Fragments arranged by size:
3507 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5635
StyI C'CwwG_G
Cuts at: 513 1065 2004 3074 513
Size: 552 939 1070 3074
Fragments arranged by size:
3074 1070 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 2635 3662 3679 3832 4017 4462
Size: 1255 990 65 1027 17 153 185 445
Cuts at: 4462 325
Size: 1498
Fragments arranged by size:
1498 1255 1027 990 445 185 153 65
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3881 5593 96
Size: 274 80 33 51 1443 1904 1712 138
Fragments arranged by size:
1904 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2554 2586 2713 2745 5595 5627 2554
Size: 32 127 32 2850 32 2562
Fragments arranged by size:
2850 2562 127 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5635
XbaI T'CTAG_A
Cuts at: 2971 2971
Size: 5635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2289 2289
Size: 5635
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5635
XmaI C'CCGG_G
Cuts at: 1870 2960 1870
Size: 1090 4545
XmnI GAAnn'nnTTC
Cuts at: 3764 3764
Size: 5635
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3443 276
Size: 53 83 186 936 1909 2468
Fragments arranged by size:
2468 1909 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI