Restriction map for
pFN21AE0433
(Circular) (Six-base) MAPSORT of: pFN21AE0433.seq Check: 8638 from: 1 to: 5947
pFN21AE0433 5947 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2981 3757 278
Size: 53 83 186 936 1445 776 2468
Fragments arranged by size:
2468 1445 936 776 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 3166 4074 4447 1563
Size: 1603 908 373 3063
Fragments arranged by size:
3063 1603 908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2311 2672 4010 5394 5662 1632
Size: 679 361 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 679 361 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5750
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 2833 4676 2833
Size: 1843 4104
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5947
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5947
AlwNI CAG_nnn'CTG
Cuts at: 2924 3690 5262 2924
Size: 766 1572 3609
Fragments arranged by size:
3609 1572 766
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 3256 3482 3634 5743 1493
Size: 1763 226 152 2109 1697
Fragments arranged by size:
2109 1763 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2294 3268 4723 618
Size: 325 217 627 507 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 507 325 217
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2317 928
Size: 971 418 4558
Fragments arranged by size:
4558 971 418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3839 5049 3839
Size: 1210 4737
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2733 5942 2733
Size: 3209 2738
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 4008 5164 1790
Size: 2218 1156 2573
Fragments arranged by size:
2573 2218 1156
BmgBI CAC'GTC
Cuts at: 1318 2830 1318
Size: 1512 4435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2710 3678 4636
Size: 416 53 83 399 24 1293 968 958
Cuts at: 4636 442
Size: 1753
Fragments arranged by size:
1753 1293 968 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4607 1239
Size: 3368 2579
Bpu10I CC'TnA_GC
Cuts at: 3234 3234
Size: 5947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4072 4937 5235 4072
Size: 865 298 4784
Fragments arranged by size:
4784 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4610 882
Size: 617 3111 2219
Fragments arranged by size:
3111 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2107 3738 493
Size: 711 903 1631 2702
Fragments arranged by size:
2702 1631 903 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 2978 3754 4136
Size: 53 83 186 936 681 764 776 382
Cuts at: 4136 4771 275
Size: 635 1451
Fragments arranged by size:
1451 936 776 764 681 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2249 2279 1178
Size: 30 165 30 815 30 1 30 4846
Fragments arranged by size:
4846 815 165 30 30 30 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 1186
Size: 1084 4863
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2852 1163
Size: 573 1116 4258
Fragments arranged by size:
4258 1116 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2636 4008 4093 5164 2636
Size: 1372 85 1071 3419
Fragments arranged by size:
3419 1372 1071 85
BsmI GAATG_Cn'
Cuts at: 3458 3551 3458
Size: 93 5854
BsmBI CGTCTCn'nnnn_
Cuts at: 2969 2969
Size: 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2636 3266 4008 4093 5164 1391
Size: 399 846 630 742 85 1071 2174
Fragments arranged by size:
2174 1071 846 742 630 399 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3117 3836 5566 3117
Size: 719 1730 3498
Fragments arranged by size:
3498 1730 719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2991 4442 4616 65
Size: 1183 1743 1451 174 1396
Fragments arranged by size:
1743 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4591 4778 1700
Size: 110 2781 187 2869
Fragments arranged by size:
2869 2781 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2924 794
Size: 2130 3817
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstXI CCAn_nnnn'nTGG
Cuts at: 2858 2887 3130 2858
Size: 29 243 5675
Fragments arranged by size:
5675 243 29
BstYI r'GATC_y
Cuts at: 1972 2116 2733 3277 3644 4029 4046 5498 5584
Size: 144 617 544 367 385 17 1452 86
Cuts at: 5584 5596 5942 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 617 544 385 367 346 144
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2541 2929 513
Size: 552 1476 388 3531
Fragments arranged by size:
3531 1476 552 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2674 3251 2674
Size: 577 5370
DraIII CAC_nnn'GTG
Cuts at: 2480 2480
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 3877 1353
Size: 887 1637 3423
Fragments arranged by size:
3423 1637 887
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2672 4010 4607 5394 5662 1239
Size: 393 679 361 1338 597 787 268 1524
Fragments arranged by size:
1524 1338 787 679 597 393 361 268
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2539 2571 2784 2816 2819
Size: 32 135 32 967 32 213 32 3
Cuts at: 2819 2851 4588 4620 4662 4694 1373
Size: 32 1737 32 42 32 2626
Fragments arranged by size:
2626 1737 967 213 135 42 32 32
32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3038 748
Size: 2290 3657
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 2568 3102 3546 2568
Size: 534 444 4969
Fragments arranged by size:
4969 534 444
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2817 5061 5245 1305
Size: 1512 2244 184 2007
Fragments arranged by size:
2244 2007 1512 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 2406 2546 3905 4264 4423 518
Size: 648 961 279 140 1359 359 159 2042
Fragments arranged by size:
2042 1359 961 648 359 279 159 140
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2541 513
Size: 552 1476 3919
Fragments arranged by size:
3919 1476 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 2959 3305 4850 1132
Size: 97 1730 346 1545 2229
Fragments arranged by size:
2229 1730 1545 346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5947
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5947
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 2188 3299 830
Size: 1358 1111 3478
Fragments arranged by size:
3478 1358 1111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SexAI A'CCwGG_T
Cuts at: 2505 2505
Size: 5947
SfcI C'TryA_G
Cuts at: 826 1045 2184 3295 4430 5111 5302 826
Size: 219 1139 1111 1135 681 191 1471
Fragments arranged by size:
1471 1139 1135 1111 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4051 4952 5214 820
Size: 197 926 2108 901 262 1553
Fragments arranged by size:
2108 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 2308 3871 5
Size: 47 2256 1563 2081
Fragments arranged by size:
2256 2081 1563 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2175 2541 2851 3123 3386 513
Size: 552 1110 366 310 272 263 3074
Fragments arranged by size:
3074 1110 552 366 310 272 263
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 3974 3991 4144 4329 4774 325
Size: 1255 723 1671 17 153 185 445 1498
Fragments arranged by size:
1671 1498 1255 723 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2706 4193 5905
Size: 274 80 33 51 1443 729 1487 1712
Cuts at: 5905 96
Size: 138
Fragments arranged by size:
1712 1487 1443 729 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2218 2250 5907 5939 2218
Size: 32 3657 32 2226
Fragments arranged by size:
3657 2226 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2168 1316
Size: 852 5095
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 4076 4076
Size: 5947
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2979 3755 276
Size: 53 83 186 936 1445 776 2468
Fragments arranged by size:
2468 1445 936 776 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI