Restriction map for
pFN21AE0429
(Circular) (Six-base) MAPSORT of: pFN21AE0429.seq Check: 2729 from: 1 to: 5989
pFN21AE0429 5989 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3220 3220
Size: 5989
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3799 278
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3332 1937
Size: 1395 4594
Acc65I G'GTAC_C
Cuts at: 2670 3310 2670
Size: 640 5349
AclI AA'CG_TT
Cuts at: 1563 4116 4489 1563
Size: 2553 373 3063
Fragments arranged by size:
3063 2553 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2484 4052 5436 5704 1632
Size: 852 1568 1384 268 1917
Fragments arranged by size:
1917 1568 1384 852 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5792
AflIII A'CryG_T
Cuts at: 1203 4888 5865 1203
Size: 3685 977 1327
Fragments arranged by size:
3685 1327 977
AgeI A'CCGG_T
Cuts at: 4820 4820
Size: 5989
AhdI GACnn_n'nnGTC
Cuts at: 4718 4718
Size: 5989
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5989
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 5957
AlwNI CAG_nnn'CTG
Cuts at: 3732 5304 3732
Size: 1572 4417
ApaI G_GGCC'C
Cuts at: 2024 2107 2268 2024
Size: 83 161 5745
Fragments arranged by size:
5745 161 83
ApaLI G'TGCA_C
Cuts at: 2733 4046 5202 2733
Size: 1313 1156 3520
Fragments arranged by size:
3520 1313 1156
ApoI r'AATT_y
Cuts at: 1493 3298 3524 3676 5785 1493
Size: 1805 226 152 2109 1697
Fragments arranged by size:
2109 1805 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4543 160
Size: 872 3511 1606
Fragments arranged by size:
3511 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2261 2267 3314 1870
Size: 73 246 72 6 1047 4545
Fragments arranged by size:
4545 1047 246 73 72 6
BamHI G'GATC_C
Cuts at: 3319 3686 3319
Size: 367 5622
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2670 3232 3310 4765 618
Size: 325 217 627 883 562 78 1455 1842
Fragments arranged by size:
1842 1455 883 627 562 325 217 78
BanII G_rGCy'C
Cuts at: 1391 2024 2107 2268 2348 3308 1391
Size: 633 83 161 80 960 4072
Fragments arranged by size:
4072 960 633 161 83 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2694 928
Size: 971 795 4223
Fragments arranged by size:
4223 971 795
BbvCI CC'TCA_GC
Cuts at: 3213 3213
Size: 5989
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4180 4214 1903
Size: 34 2243 34 3678
Fragments arranged by size:
3678 2243 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3881 5091 3881
Size: 1210 4779
BclI T'GATC_A
Cuts at: 1548 2173 1548
Size: 625 5364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2626 4600 136
Size: 107 122 71 631 605 954 1974 1525
Fragments arranged by size:
1974 1525 954 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5984 5984
Size: 5989
BlpI GC'TnA_GC
Cuts at: 3406 3406
Size: 5989
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2107 2262 2268 2737 3237 3284 4050
Size: 234 83 155 6 469 500 47 766
Cuts at: 4050 5206 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 766 500 469 234 155 83
47 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5989
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2506 2589 2841
Size: 416 53 83 399 24 1089 83 252
Cuts at: 2841 3720 4678 442
Size: 879 958 1753
Fragments arranged by size:
1753 1089 958 879 416 399 252 83
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 3200 4649 1239
Size: 1117 844 1449 2579
Fragments arranged by size:
2579 1449 1117 844
Bpu10I CC'TnA_GC
Cuts at: 2133 2908 3213 2133
Size: 775 305 4909
Fragments arranged by size:
4909 775 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 4114 4979 5277 2093
Size: 2021 865 298 2805
Fragments arranged by size:
2805 2021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4652 882
Size: 617 3153 2219
Fragments arranged by size:
3153 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3780 493
Size: 711 2576 2702
Fragments arranged by size:
2702 2576 711
BsaBI GATnn'nnATC
Cuts at: 5983 5983
Size: 5989
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3796 4178 4813 275
Size: 53 83 186 936 2263 382 635 1451
Fragments arranged by size:
2263 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4422 4820 5094 5241 5652 4422
Size: 398 274 147 411 4759
Fragments arranged by size:
4759 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3100 3130 1178
Size: 30 165 30 1697 30 4037
Fragments arranged by size:
4037 1697 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2431 2560 2974 2977 1186
Size: 1245 129 414 3 4198
Fragments arranged by size:
4198 1245 414 129 3
BseYI C'CCAG_C
Cuts at: 2665 3064 5192 5688 2665
Size: 399 2128 496 2966
Fragments arranged by size:
2966 2128 496 399
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 1163
Size: 573 599 4817
Fragments arranged by size:
4817 599 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3439 4200 5228 1540
Size: 275 186 1438 761 1028 2301
Fragments arranged by size:
2301 1438 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2348 2737 4050 4135 5206 2348
Size: 389 1313 85 1071 3131
Fragments arranged by size:
3131 1313 1071 389 85
BsmI GAATG_Cn'
Cuts at: 3500 3593 3500
Size: 93 5896
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2107 2262 2268 2348 2737 3237
Size: 399 234 83 155 6 80 389 500
Cuts at: 3237 3284 3308 4050 4135 5206 1391
Size: 47 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 389 234 155
85 83 80 47 24 6
BspEI T'CCGG_A
Cuts at: 5652 5652
Size: 5989
BspHI T'CATG_A
Cuts at: 3878 5608 3878
Size: 1730 4259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2731 3220 3344 844
Size: 1887 489 124 3489
Fragments arranged by size:
3489 1887 489 124
BsrBI CCG'CTC
Cuts at: 1394 1823 2118 3147 3876 1394
Size: 429 295 1029 729 3507
Fragments arranged by size:
3507 1029 729 429 295
BsrDI GCAATG_nn'
Cuts at: 65 1248 4484 4658 65
Size: 1183 3236 174 1396
Fragments arranged by size:
3236 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4633 4820 1700
Size: 110 2823 187 2869
Fragments arranged by size:
2869 2823 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5989
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5989
BssSI C'ACGA_G
Cuts at: 3277 4049 5061 3277
Size: 772 1012 4205
Fragments arranged by size:
4205 1012 772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2213 2918 3232 794
Size: 1419 705 314 3551
Fragments arranged by size:
3551 1419 705 314
BstBI TT'CG_AA
Cuts at: 4793 4793
Size: 5989
BstEII G'GTnAC_C
Cuts at: 1184 2194 3037 1184
Size: 1010 843 4136
Fragments arranged by size:
4136 1010 843
BstYI r'GATC_y
Cuts at: 1972 2992 3254 3319 3686 4071 4088 5540 5626
Size: 1020 262 65 367 385 17 1452 86
Cuts at: 5626 5638 5984 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1020 385 367 346 262 86
65 17 12
Bsu36I CC'TnA_GG
Cuts at: 2487 2558 2487
Size: 71 5918
BtgI C'CryG_G
Cuts at: 513 1065 2004 2388 513
Size: 552 939 384 4114
Fragments arranged by size:
4114 939 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5250
Fragments arranged by size:
5250 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 2735 3505 4299 4319 2437
Size: 298 770 794 20 4107
Fragments arranged by size:
4107 794 770 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5954
DraI TTT'AAA
Cuts at: 3293 3293
Size: 5989
DraIII CAC_nnn'GTG
Cuts at: 2514 2514
Size: 5989
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2153 2875 4996 809
Size: 507 837 722 2121 1802
Fragments arranged by size:
2121 1802 837 722 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3436 4325 5954
Size: 54 1425 50 275 18 1606 889 1629
Cuts at: 5954 8
Size: 43
Fragments arranged by size:
1629 1606 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3436 1537
Size: 275 1624 4090
Fragments arranged by size:
4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2503 3016 3919 1353
Size: 1150 513 903 3423
Fragments arranged by size:
3423 1150 903 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4576 4950 5096 387
Size: 1064 138 2987 374 146 1280
Fragments arranged by size:
2987 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2346 2346
Size: 5989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2484 3200 4052 4649 5436 5704
Size: 393 724 128 716 852 597 787 268
Cuts at: 5704 1239
Size: 1524
Fragments arranged by size:
1524 852 787 724 716 597 393 268
128
EcoO109I rG'GnC_Cy
Cuts at: 2020 2103 2104 2146 2185 2926 2972 3193 2020
Size: 83 1 42 39 741 46 221 4816
Fragments arranged by size:
4816 741 221 83 46 42 39 1
EcoRI G'AATT_C
Cuts at: 3298 3676 3298
Size: 378 5611
FspI TGC'GCA
Cuts at: 4495 4495
Size: 5989
HaeII r_GCGC'y
Cuts at: 1678 5136 1678
Size: 3458 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373
Size: 32 135 32 3058 32 42 32 2626
Fragments arranged by size:
3058 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3333 3579 669
Size: 988 281 1395 246 3079
Fragments arranged by size:
3079 1395 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5989
HpaI GTT'AAC
Cuts at: 3579 3579
Size: 5989
KpnI G_GTAC'C
Cuts at: 2674 3314 2674
Size: 640 5349
MfeI C'AATT_G
Cuts at: 3588 3588
Size: 5989
MluI A'CGCG_T
Cuts at: 5865 5865
Size: 5989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5103 5287 1305
Size: 3798 184 2007
Fragments arranged by size:
3798 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4167
Fragments arranged by size:
4167 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3947 4306 4465 518
Size: 648 2781 359 159 2042
Fragments arranged by size:
2781 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2446 3068 3125 3223 3405 4082 5230 5475
Size: 840 622 57 98 182 677 1148 245
Cuts at: 5475 1606
Size: 2120
Fragments arranged by size:
2120 1148 840 677 622 245 182 98
57
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4498
Fragments arranged by size:
4498 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5989
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5989
NotI GC'GGCC_GC
Cuts at: 3436 3436
Size: 5989
NspI r_CATG'y
Cuts at: 1132 1229 3347 4892 1132
Size: 97 2118 1545 2229
Fragments arranged by size:
2229 2118 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5989
PciI A'CATG_T
Cuts at: 4888 4888
Size: 5989
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5989
PfoI T'CCnGG_A
Cuts at: 2336 3266 2336
Size: 930 5059
PmeI GTTT'AAAC
Cuts at: 3293 3293
Size: 5989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4058 4090 5571 5603 4058
Size: 32 1481 32 4444
Fragments arranged by size:
4444 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2185 2926 2146
Size: 39 741 5209
Fragments arranged by size:
5209 741 39
PshAI GACnn'nnGTC
Cuts at: 1613 2079 1613
Size: 466 5523
PsiI TTA'TAA
Cuts at: 3559 3559
Size: 5989
PspOMI G'GGCC_C
Cuts at: 2020 2103 2264 2020
Size: 83 161 5745
Fragments arranged by size:
5745 161 83
PstI C_TGCA'G
Cuts at: 830 2280 2742 2771 2923 3124 3228 3341 830
Size: 1450 462 29 152 201 104 113 3478
Fragments arranged by size:
3478 1450 462 201 152 113 104 29
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5989
PvuII CAG'CTG
Cuts at: 2446 3068 3125 3223 2446
Size: 622 57 98 5212
Fragments arranged by size:
5212 622 98 57
SacI G_AGCT'C
Cuts at: 2348 2348
Size: 5989
SalI G'TCGA_C
Cuts at: 1936 3331 1936
Size: 1395 4594
SanDI GG'GwC_CC
Cuts at: 2185 2926 2185
Size: 741 5248
SbfI CC_TGCA'GG
Cuts at: 3341 3341
Size: 5989
ScaI AGT'ACT
Cuts at: 4237 5949 4237
Size: 1712 4277
SfcI C'TryA_G
Cuts at: 826 1045 2276 2380 2738 2767 2919 2934 3120
Size: 219 1231 104 358 29 152 15 186
Cuts at: 3120 3224 3337 4472 5153 5344 826
Size: 104 113 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 358 219 191 186
152 113 104 104 29 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5989
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5989
SmaI CCC'GGG
Cuts at: 1872 2263 2269 3316 1872
Size: 391 6 1047 4545
Fragments arranged by size:
4545 1047 391 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 4093 4994 5256 820
Size: 197 926 165 1985 901 262 1553
Fragments arranged by size:
1985 1553 926 901 262 197 165
SnaBI TAC'GTA
Cuts at: 493 3780 493
Size: 3287 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5989
SphI G_CATG'C
Cuts at: 1132 3347 1132
Size: 2215 3774
SrfI GCCC'GGGC
Cuts at: 2263 2269 2263
Size: 6 5983
SspI AAT'ATT
Cuts at: 5 52 3913 5
Size: 47 3861 2081
Fragments arranged by size:
3861 2081 47
StuI AGG'CCT
Cuts at: 1517 2244 2485 2585 1517
Size: 727 241 100 4921
Fragments arranged by size:
4921 727 241 100
StyI C'CwwG_G
Cuts at: 513 1065 2004 3428 513
Size: 552 939 1424 3074
Fragments arranged by size:
3074 1424 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2525 2860 4016 4033 4186 4371 4816
Size: 1255 945 335 1156 17 153 185 445
Cuts at: 4816 325
Size: 1498
Fragments arranged by size:
1498 1255 1156 945 445 335 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2597 4235 5947
Size: 274 80 33 51 1443 620 1638 1712
Cuts at: 5947 96
Size: 138
Fragments arranged by size:
1712 1638 1443 620 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2780 2812 3100 3132 5949 5981 2000
Size: 32 748 32 288 32 2817 32 2008
Fragments arranged by size:
2817 2008 748 288 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5989
XbaI T'CTAG_A
Cuts at: 3325 3325
Size: 5989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 5989
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5989
XmaI C'CCGG_G
Cuts at: 1870 2261 2267 3314 1870
Size: 391 6 1047 4545
Fragments arranged by size:
4545 1047 391 6
XmnI GAAnn'nnTTC
Cuts at: 4118 4118
Size: 5989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3797 276
Size: 53 83 186 936 2263 2468
Fragments arranged by size:
2468 2263 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SwaI