Restriction map for
pFN21AE0424
(Circular) (Six-base) MAPSORT of: pFN21AE0424.seq Check: 2743 from: 1 to: 6115
pFN21AE0424 6115 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 3458 1937
Size: 475 1046 4594
Fragments arranged by size:
4594 1046 475
Acc65I G'GTAC_C
Cuts at: 2957 3436 2957
Size: 479 5636
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 3179 4178 5562 5830 1632
Size: 893 654 999 1384 268 1917
Fragments arranged by size:
1917 1384 999 893 654 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6115
AlwNI CAG_nnn'CTG
Cuts at: 2623 3008 3255 3858 5430 2623
Size: 385 247 603 1572 3308
Fragments arranged by size:
3308 1572 603 385 247
ApaI G_GGCC'C
Cuts at: 2818 3096 3389 2818
Size: 278 293 5544
Fragments arranged by size:
5544 293 278
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2459 3440 1870
Size: 73 85 431 981 4545
Fragments arranged by size:
4545 981 431 85 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2949 2982 2949
Size: 33 6082
BamHI G'GATC_C
Cuts at: 2802 3445 3812 2802
Size: 643 367 5105
Fragments arranged by size:
5105 643 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2575 2957 3436 4891 618
Size: 325 217 627 788 382 479 1455 1842
Fragments arranged by size:
1842 1455 788 627 479 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2179 2818 3096 3389 3434 1391
Size: 788 639 278 293 45 4072
Fragments arranged by size:
4072 788 639 293 278 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5144
BbvCI CC'TCA_GC
Cuts at: 3347 3347
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2402 2436 4306 4340 1903
Size: 34 465 34 1870 34 3678
Fragments arranged by size:
3678 1870 465 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2711 4726
Size: 107 122 71 631 605 337 702 2015
Cuts at: 4726 136
Size: 1525
Fragments arranged by size:
2015 1525 702 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 2294 3532 2294
Size: 1238 4877
Bme1580I G_kGCm'C
Cuts at: 1790 2818 3096 3389 4176 5332 1790
Size: 1028 278 293 787 1156 2573
Fragments arranged by size:
2573 1156 1028 787 293 278
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2996 3846 4804
Size: 416 53 83 399 24 1579 850 958
Cuts at: 4804 442
Size: 1753
Fragments arranged by size:
1753 1579 958 850 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 4775 1239
Size: 1031 2505 2579
Fragments arranged by size:
2579 2505 1031
Bpu10I CC'TnA_GC
Cuts at: 2624 3134 3347 2624
Size: 510 213 5392
Fragments arranged by size:
5392 510 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 3338 4240 5105 5403 2618
Size: 720 902 865 298 3330
Fragments arranged by size:
3330 902 865 720 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4778 882
Size: 617 3279 2219
Fragments arranged by size:
3279 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3906 493
Size: 711 2702 2702
Fragments arranged by size:
2702 2702 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3922 4304 4939 275
Size: 53 83 186 936 2389 382 635 1451
Fragments arranged by size:
2389 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4548 4946 5220 5367 5778 4548
Size: 398 274 147 411 4885
Fragments arranged by size:
4885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2380 3143 1186
Size: 1194 763 4158
Fragments arranged by size:
4158 1194 763
BseYI C'CCAG_C
Cuts at: 2254 3095 5318 5814 2254
Size: 841 2223 496 2555
Fragments arranged by size:
2555 2223 841 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2900 3320 1163
Size: 573 1164 420 3958
Fragments arranged by size:
3958 1164 573 420
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2187 3565 4326 5354 1540
Size: 275 186 186 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 275 186 186
BsiHKAI G_wGCw'C
Cuts at: 2630 4176 4261 5332 2630
Size: 1546 85 1071 3413
Fragments arranged by size:
3413 1546 1071 85
BsmI GAATG_Cn'
Cuts at: 2374 3626 3719 2374
Size: 1252 93 4770
Fragments arranged by size:
4770 1252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2630 2818 3096 3389 3434 4176
Size: 399 389 451 188 278 293 45 742
Cuts at: 4176 4261 5332 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 389 293 278
188 85 45
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 2194 4004 5734 2194
Size: 1810 1730 2575
Fragments arranged by size:
2575 1810 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 2454 2608 3470 844
Size: 1281 329 154 862 3489
Fragments arranged by size:
3489 1281 862 329 154
BsrBI CCG'CTC
Cuts at: 1394 1823 2795 4002 1394
Size: 429 972 1207 3507
Fragments arranged by size:
3507 1207 972 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3058 4610 4784 65
Size: 1183 1810 1552 174 1396
Fragments arranged by size:
1810 1552 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 4759 4946 1700
Size: 110 273 2676 187 2869
Fragments arranged by size:
2869 2676 273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 3155 4175 5187 3155
Size: 1020 1012 4083
Fragments arranged by size:
4083 1020 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3190 794
Size: 2396 3719
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstXI CCAn_nnnn'nTGG
Cuts at: 3053 3053
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2239 2802 3207 3445 3812 4197 4214 5666
Size: 267 563 405 238 367 385 17 1452
Cuts at: 5666 5752 5764 6110 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 563 405 385 367 346 267
238 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2045 2075 2706 3027 3360 513
Size: 552 951 29 30 631 321 333 3268
Fragments arranged by size:
3268 951 631 552 333 321 30 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 3112 3631 4425 4445 3112
Size: 519 794 20 4782
Fragments arranged by size:
4782 794 519 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2184 2487
Size: 54 1425 50 275 18 183 171 303
Cuts at: 2487 2712 2985 3562 4451 6080 8
Size: 225 273 577 889 1629 43
Fragments arranged by size:
1629 1425 889 577 303 275 273 225
183 171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2184 3562 1537
Size: 275 372 1378 4090
Fragments arranged by size:
4090 1378 372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3320 4045 1353
Size: 1967 725 3423
Fragments arranged by size:
3423 1967 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2141 2666 4702 5076 5222 387
Size: 1064 138 552 525 2036 374 146 1280
Fragments arranged by size:
2036 1280 1064 552 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2270 2525 3179 4178 4775 5562 5830
Size: 393 638 255 654 999 597 787 268
Cuts at: 5830 1239
Size: 1524
Fragments arranged by size:
1524 999 787 654 638 597 393 268
255
EcoNI CCTnn'n_nnAGG
Cuts at: 2923 2923
Size: 6115
EcoO109I rG'GnC_Cy
Cuts at: 2137 2258 2620 2854 3092 3385 3386 2137
Size: 121 362 234 238 293 1 4866
Fragments arranged by size:
4866 362 293 238 234 121 1
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 2063 2352 3037 5262 1678
Size: 385 289 685 2225 2531
Fragments arranged by size:
2531 2225 685 385 289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2755 2787 3349 3381 4756
Size: 32 135 32 1183 32 562 32 1375
Cuts at: 4756 4788 4830 4862 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1375 1183 562 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2413 3459 3705 669
Size: 988 281 475 1046 246 3079
Fragments arranged by size:
3079 1046 988 475 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KpnI G_GTAC'C
Cuts at: 2961 3440 2961
Size: 479 5636
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5229 5413 1305
Size: 3924 184 2007
Fragments arranged by size:
3924 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2489 2987 10
Size: 54 1425 343 657 498 3138
Fragments arranged by size:
3138 1425 657 498 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 3284 4073 4432 4591 518
Size: 648 1885 233 789 359 159 2042
Fragments arranged by size:
2042 1885 789 648 359 233 159
MspA1I CmG'CkG
Cuts at: 1606 2047 2123 2143 2660 2672 2734 3531 4208
Size: 441 76 20 517 12 62 797 677
Cuts at: 4208 5356 5601 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 517 441 245 76
62 20 12
NcoI C'CATG_G
Cuts at: 513 1065 2016 3027 3360 513
Size: 552 951 1011 333 3268
Fragments arranged by size:
3268 1011 951 552 333
NdeI CA'TA_TG
Cuts at: 387 2317 387
Size: 1930 4185
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 2074 2480 3473 5018 1132
Size: 97 845 406 993 1545 2229
Fragments arranged by size:
2229 1545 993 845 406 97
PasI CC'CwG_GG
Cuts at: 1348 2580 1348
Size: 1232 4883
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3412 1108
Size: 2304 3811
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2258 2620 2854 2137
Size: 121 362 234 5398
Fragments arranged by size:
5398 362 234 121
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 2814 3092 3385 2814
Size: 278 293 5544
Fragments arranged by size:
5544 293 278
PspXI vC'TCGA_Gb
Cuts at: 2028 2028
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2286 2451 3467 830
Size: 1456 165 1016 3478
Fragments arranged by size:
3478 1456 1016 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2734 2734
Size: 6115
RsrII CG'GwC_CG
Cuts at: 2674 2674
Size: 6115
SacII CC_GC'GG
Cuts at: 2048 2048
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 2411 3457 1936
Size: 475 1046 4594
Fragments arranged by size:
4594 1046 475
SanDI GG'GwC_CC
Cuts at: 2258 2258
Size: 6115
SapI GCTCTTCn'nnn_
Cuts at: 3320 3320
Size: 6115
SbfI CC_TGCA'GG
Cuts at: 2286 2451 3467 2286
Size: 165 1016 4934
Fragments arranged by size:
4934 1016 165
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2282 2447 3463 4598 5279 5470 826
Size: 219 1237 165 1016 1135 681 191 1471
Fragments arranged by size:
1471 1237 1135 1016 681 219 191 165
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 3442 1872
Size: 1570 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2028 2633 3353 4219 5120 5382
Size: 197 926 85 605 720 866 901 262
Cuts at: 5382 820
Size: 1553
Fragments arranged by size:
1553 926 901 866 720 605 262 197
85
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 2074 3473 1132
Size: 942 1399 3774
Fragments arranged by size:
3774 1399 942
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2016 3027 3360 3554 513
Size: 552 951 1011 333 194 3074
Fragments arranged by size:
3074 1011 951 552 333 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2593 4142 4159 4312 4497 4942 325
Size: 1255 1013 1549 17 153 185 445 1498
Fragments arranged by size:
1549 1498 1255 1013 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2748 2780 6075 6107 2748
Size: 32 3295 32 2756
Fragments arranged by size:
3295 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 2028 1943
Size: 85 6030
XmaI C'CCGG_G
Cuts at: 1870 3440 1870
Size: 1570 4545
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PsrI SacI SexAI SfiI SfoI SrfI
SwaI XcmI