Restriction map for
pFN21AE0421
(Circular) (Six-base) MAPSORT of: pFN21AE0421.seq Check: 4091 from: 1 to: 5272
pFN21AE0421 5272 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3082 278
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2615 1937
Size: 678 4594
Acc65I G'GTAC_C
Cuts at: 2593 2593
Size: 5272
AclI AA'CG_TT
Cuts at: 1563 3399 3772 1563
Size: 1836 373 3063
Fragments arranged by size:
3063 1836 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2345 2462 3335 4719 4987 1632
Size: 585 128 117 873 1384 268 1917
Fragments arranged by size:
1917 1384 873 585 268 128 117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5075
AflIII A'CryG_T
Cuts at: 1203 2089 4171 5148 1203
Size: 886 2082 977 1327
Fragments arranged by size:
2082 1327 977 886
AgeI A'CCGG_T
Cuts at: 4103 4103
Size: 5272
AhdI GACnn_n'nnGTC
Cuts at: 4001 4001
Size: 5272
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5272
AlwNI CAG_nnn'CTG
Cuts at: 3015 4587 3015
Size: 1572 3700
ApaLI G'TGCA_C
Cuts at: 2290 3329 4485 2290
Size: 1039 1156 3077
Fragments arranged by size:
3077 1156 1039
ApoI r'AATT_y
Cuts at: 1493 2152 2177 2581 2807 2959 5068 1493
Size: 659 25 404 226 152 2109 1697
Fragments arranged by size:
2109 1697 659 404 226 152 25
AseI AT'TA_AT
Cuts at: 160 1032 3826 160
Size: 872 2794 1606
Fragments arranged by size:
2794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 1870
Size: 73 654 4545
Fragments arranged by size:
4545 654 73
BamHI G'GATC_C
Cuts at: 2602 2969 2602
Size: 367 4905
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2593 4048 618
Size: 325 217 627 806 1455 1842
Fragments arranged by size:
1842 1455 806 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2591 1391
Size: 1200 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4301
BbvCI CC'TCA_GC
Cuts at: 2490 2490
Size: 5272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3463 3497 1903
Size: 34 1526 34 3678
Fragments arranged by size:
3678 1526 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3164 4374 3164
Size: 1210 4062
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5272
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2200 3883 136
Size: 107 122 71 631 605 528 1683 1525
Fragments arranged by size:
1683 1525 631 605 528 122 107 71
BglII A'GATC_T
Cuts at: 5267 5267
Size: 5272
BlpI GC'TnA_GC
Cuts at: 2689 2689
Size: 5272
Bme1580I G_kGCm'C
Cuts at: 1790 2294 3333 4489 1790
Size: 504 1039 1156 2573
Fragments arranged by size:
2573 1156 1039 504
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5272
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2388 2541 3003
Size: 416 53 83 399 24 971 153 462
Cuts at: 3003 3961 442
Size: 958 1753
Fragments arranged by size:
1753 971 958 462 416 399 153 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2126 2126
Size: 5272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3932 1239
Size: 2693 2579
Bpu10I CC'TnA_GC
Cuts at: 2490 2490
Size: 5272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3397 4262 4560 3397
Size: 865 298 4109
Fragments arranged by size:
4109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3935 882
Size: 617 2436 2219
Fragments arranged by size:
2436 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2024 2090 3063 493
Size: 711 820 66 973 2702
Fragments arranged by size:
2702 973 820 711 66
BsaBI GATnn'nnATC
Cuts at: 5266 5266
Size: 5272
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3079 3461 4096 275
Size: 53 83 186 936 1546 382 635 1451
Fragments arranged by size:
1546 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3705 4103 4377 4524 4935 3705
Size: 398 274 147 411 4042
Fragments arranged by size:
4042 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 1178
Size: 30 165 30 1083 30 3934
Fragments arranged by size:
3934 1083 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 1186
Size: 1321 3951
BseYI C'CCAG_C
Cuts at: 2259 4475 4971 2259
Size: 2216 496 2560
Fragments arranged by size:
2560 2216 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4699
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2722 3483 4511 1540
Size: 275 186 721 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 2294 3333 3418 4489 2294
Size: 1039 85 1071 3077
Fragments arranged by size:
3077 1071 1039 85
BsmI GAATG_Cn'
Cuts at: 2783 2876 2783
Size: 93 5179
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2294 2591 3333 3418 4489 1391
Size: 399 504 297 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 297 85
BspEI T'CCGG_A
Cuts at: 4935 4935
Size: 5272
BspHI T'CATG_A
Cuts at: 3161 4891 3161
Size: 1730 3542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 844
Size: 1783 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3159 1394
Size: 429 1336 3507
Fragments arranged by size:
3507 1336 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3767 3941 65
Size: 1183 2519 174 1396
Fragments arranged by size:
2519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3916 4103 1700
Size: 110 2106 187 2869
Fragments arranged by size:
2869 2106 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5272
BssHII G'CGCG_C
Cuts at: 1925 2054 1925
Size: 129 5143
BssSI C'ACGA_G
Cuts at: 3332 4344 3332
Size: 1012 4260
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5272
BstBI TT'CG_AA
Cuts at: 4076 4076
Size: 5272
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5272
BstXI CCAn_nnnn'nTGG
Cuts at: 2315 2315
Size: 5272
BstYI r'GATC_y
Cuts at: 1972 2080 2445 2602 2969 3354 3371 4823 4909
Size: 108 365 157 367 385 17 1452 86
Cuts at: 4909 4921 5267 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 365 346 157 108
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2064 2195 513
Size: 552 939 60 131 3590
Fragments arranged by size:
3590 939 552 131 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4533
Fragments arranged by size:
4533 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 3582 3602 2788
Size: 794 20 4458
Fragments arranged by size:
4458 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5272
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2319 2354 529
Size: 35 1755 35 3447
Fragments arranged by size:
3447 1755 35 35
DraI TTT'AAA
Cuts at: 2576 2576
Size: 5272
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4279 809
Size: 507 2963 1802
Fragments arranged by size:
2963 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2719 3608
Size: 54 1425 50 275 18 291 598 889
Cuts at: 3608 5237 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 598 291 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2719 1537
Size: 275 907 4090
Fragments arranged by size:
4090 907 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3202 1353
Size: 1849 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3859 4233 4379 387
Size: 1064 138 2270 374 146 1280
Fragments arranged by size:
2270 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2345 2462 3335 3932 4719 4987
Size: 393 585 128 117 873 597 787 268
Cuts at: 4987 1239
Size: 1524
Fragments arranged by size:
1524 873 787 597 585 393 268 128
117
EcoRI G'AATT_C
Cuts at: 2581 2959 2581
Size: 378 4894
FspI TGC'GCA
Cuts at: 3778 3778
Size: 5272
HaeII r_GCGC'y
Cuts at: 1678 4419 1678
Size: 2741 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2340 2372 2486 2518 3913
Size: 32 135 32 768 32 114 32 1395
Cuts at: 3913 3945 3987 4019 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 768 135 114 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 2616 2862 669
Size: 988 281 457 221 246 3079
Fragments arranged by size:
3079 988 457 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5272
HpaI GTT'AAC
Cuts at: 2862 2862
Size: 5272
KpnI G_GTAC'C
Cuts at: 2597 2597
Size: 5272
MfeI C'AATT_G
Cuts at: 2871 2871
Size: 5272
MluI A'CGCG_T
Cuts at: 5148 5148
Size: 5272
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2296 4386 4570 1305
Size: 991 2090 184 2007
Fragments arranged by size:
2090 2007 991 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3450
Fragments arranged by size:
3450 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 3230 3589 3748 518
Size: 648 951 1113 359 159 2042
Fragments arranged by size:
2042 1113 951 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2066 2263 2336 2440 2688 3365 4513 4758
Size: 460 197 73 104 248 677 1148 245
Cuts at: 4758 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 460 248 245 197 104
73
NcoI C'CATG_G
Cuts at: 513 1065 2004 2195 513
Size: 552 939 191 3590
Fragments arranged by size:
3590 939 552 191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5272
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5272
NotI GC'GGCC_GC
Cuts at: 2719 2719
Size: 5272
NruI TCG'CGA
Cuts at: 2101 2101
Size: 5272
NspI r_CATG'y
Cuts at: 1132 1229 2630 4175 1132
Size: 97 1401 1545 2229
Fragments arranged by size:
2229 1545 1401 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5272
PciI A'CATG_T
Cuts at: 4171 4171
Size: 5272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 1108
Size: 933 4339
PmeI GTTT'AAAC
Cuts at: 2576 2576
Size: 5272
PmlI CAC'GTG
Cuts at: 2024 2024
Size: 5272
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3341 3373 4854 4886 3341
Size: 32 1481 32 3727
Fragments arranged by size:
3727 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5272
PsiI TTA'TAA
Cuts at: 2842 2842
Size: 5272
PstI C_TGCA'G
Cuts at: 830 2624 830
Size: 1794 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5272
PvuII CAG'CTG
Cuts at: 2263 2336 2440 2263
Size: 73 104 5095
Fragments arranged by size:
5095 104 73
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 5272
SalI G'TCGA_C
Cuts at: 1936 2614 1936
Size: 678 4594
SbfI CC_TGCA'GG
Cuts at: 2624 2624
Size: 5272
ScaI AGT'ACT
Cuts at: 3520 5232 3520
Size: 1712 3560
SfcI C'TryA_G
Cuts at: 826 1045 2620 3755 4436 4627 826
Size: 219 1575 1135 681 191 1471
Fragments arranged by size:
1575 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5272
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5272
SmaI CCC'GGG
Cuts at: 1872 2599 1872
Size: 727 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3376 4277 4539 820
Size: 197 926 1433 901 262 1553
Fragments arranged by size:
1553 1433 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3063 493
Size: 2570 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5272
SphI G_CATG'C
Cuts at: 1132 2630 1132
Size: 1498 3774
SspI AAT'ATT
Cuts at: 5 52 3196 5
Size: 47 3144 2081
Fragments arranged by size:
3144 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5272
StyI C'CwwG_G
Cuts at: 513 1065 2004 2195 2711 513
Size: 552 939 191 516 3074
Fragments arranged by size:
3074 939 552 516 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3299 3316 3469 3654 4099 325
Size: 1255 1719 17 153 185 445 1498
Fragments arranged by size:
1719 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3518 5230 96
Size: 274 80 33 51 1443 1541 1712 138
Fragments arranged by size:
1712 1541 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 5232 5264 2022
Size: 32 3178 32 2030
Fragments arranged by size:
3178 2030 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5272
XbaI T'CTAG_A
Cuts at: 2608 2608
Size: 5272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2267 2267
Size: 5272
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5272
XmaI C'CCGG_G
Cuts at: 1870 2597 1870
Size: 727 4545
XmnI GAAnn'nnTTC
Cuts at: 3401 3401
Size: 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3080 276
Size: 53 83 186 936 1546 2468
Fragments arranged by size:
2468 1546 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI