Restriction map for
pFN21AE0420
(Circular) (Six-base) MAPSORT of: pFN21AE0420.seq Check: 4273 from: 1 to: 5323
pFN21AE0420 5323 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2479 2479
Size: 5323
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3133 278
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2666 1937
Size: 729 4594
Acc65I G'GTAC_C
Cuts at: 2082 2644 2082
Size: 562 4761
AclI AA'CG_TT
Cuts at: 1563 3450 3823 1563
Size: 1887 373 3063
Fragments arranged by size:
3063 1887 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3386 4770 5038 1632
Size: 1754 1384 268 1917
Fragments arranged by size:
1917 1754 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5126
AflIII A'CryG_T
Cuts at: 1203 4222 5199 1203
Size: 3019 977 1327
Fragments arranged by size:
3019 1327 977
AgeI A'CCGG_T
Cuts at: 4154 4154
Size: 5323
AhdI GACnn_n'nnGTC
Cuts at: 4052 4052
Size: 5323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2172 2199 2635 1184
Size: 988 27 436 3872
Fragments arranged by size:
3872 988 436 27
AlwNI CAG_nnn'CTG
Cuts at: 3066 4638 3066
Size: 1572 3751
ApaLI G'TGCA_C
Cuts at: 3380 4536 3380
Size: 1156 4167
ApoI r'AATT_y
Cuts at: 1493 2632 2858 3010 5119 1493
Size: 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3877 160
Size: 872 2845 1606
Fragments arranged by size:
2845 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 1870
Size: 73 705 4545
Fragments arranged by size:
4545 705 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2179 2212 2179
Size: 33 5290
BamHI G'GATC_C
Cuts at: 2653 3020 2653
Size: 367 4956
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 2240 2644 4099 618
Size: 325 217 627 295 158 404 1455 1842
Fragments arranged by size:
1842 1455 627 404 325 295 217 158
BanII G_rGCy'C
Cuts at: 1391 2340 2619 2642 1391
Size: 949 279 23 4072
Fragments arranged by size:
4072 949 279 23
BbeI G_GCGC'C
Cuts at: 2244 2244
Size: 5323
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 928
Size: 971 405 3947
Fragments arranged by size:
3947 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3514 3548 1903
Size: 34 1577 34 3678
Fragments arranged by size:
3678 1577 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3215 4425 3215
Size: 1210 4113
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3934 136
Size: 107 122 71 631 605 2262 1525
Fragments arranged by size:
2262 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2290 5318 2290
Size: 3028 2295
BlpI GC'TnA_GC
Cuts at: 2740 2740
Size: 5323
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3384 4540 1790
Size: 289 1305 1156 2573
Fragments arranged by size:
2573 1305 1156 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5323
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3054 4012 442
Size: 416 53 83 399 24 1637 958 1753
Fragments arranged by size:
1753 1637 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2585 3983 1239
Size: 926 420 1398 2579
Fragments arranged by size:
2579 1398 926 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3448 4313 4611 3448
Size: 865 298 4160
Fragments arranged by size:
4160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3986 882
Size: 617 2487 2219
Fragments arranged by size:
2487 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2581 3114 493
Size: 711 1377 533 2702
Fragments arranged by size:
2702 1377 711 533
BsaBI GATnn'nnATC
Cuts at: 5317 5317
Size: 5323
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3130 3512 4147
Size: 53 83 186 936 708 889 382 635
Cuts at: 4147 275
Size: 1451
Fragments arranged by size:
1451 936 889 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2460 3756 4154 4428 4575 4986 2460
Size: 1296 398 274 147 411 2797
Fragments arranged by size:
2797 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 1178
Size: 30 165 30 735 30 4333
Fragments arranged by size:
4333 735 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 4389
BseYI C'CCAG_C
Cuts at: 2032 2232 2339 4526 5022 2032
Size: 200 107 2187 496 2333
Fragments arranged by size:
2333 2187 496 200 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2188 1163
Size: 573 452 4298
Fragments arranged by size:
4298 573 452
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2773 3534 4562 1540
Size: 275 186 772 761 1028 2301
Fragments arranged by size:
2301 1028 772 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3384 3469 4540 3384
Size: 85 1071 4167
Fragments arranged by size:
4167 1071 85
BsmI GAATG_Cn'
Cuts at: 2834 2927 2834
Size: 93 5230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2340 2619 2642 3384 3469 4540
Size: 399 289 261 279 23 742 85 1071
Cuts at: 4540 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 289 279 261 85
23
BspEI T'CCGG_A
Cuts at: 2460 4986 2460
Size: 2526 2797
BspHI T'CATG_A
Cuts at: 2325 3212 4942 2325
Size: 887 1730 2706
Fragments arranged by size:
2706 1730 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 2678 844
Size: 1635 199 3489
Fragments arranged by size:
3489 1635 199
BsrBI CCG'CTC
Cuts at: 1394 1823 3210 1394
Size: 429 1387 3507
Fragments arranged by size:
3507 1387 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3818 3992 65
Size: 1183 2570 174 1396
Fragments arranged by size:
2570 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3967 4154 1700
Size: 110 2157 187 2869
Fragments arranged by size:
2869 2157 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5323
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5323
BssSI C'ACGA_G
Cuts at: 2087 3383 4395 2087
Size: 1296 1012 3015
Fragments arranged by size:
3015 1296 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5323
BstBI TT'CG_AA
Cuts at: 4127 4127
Size: 5323
BstEII G'GTnAC_C
Cuts at: 1184 2518 1184
Size: 1334 3989
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 5323
BstYI r'GATC_y
Cuts at: 1972 2290 2653 3020 3405 3422 4874 4960 4972
Size: 318 363 367 385 17 1452 86 12
Cuts at: 4972 5318 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 363 346 318 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4771
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2396 487
Size: 683 56 1170 3414
Fragments arranged by size:
3414 1170 683 56
BtsI GCAGTG_nn'
Cuts at: 2171 2509 2839 3633 3653 2171
Size: 338 330 794 20 3841
Fragments arranged by size:
3841 794 338 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5323
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5288
DraI TTT'AAA
Cuts at: 2627 2627
Size: 5323
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4330 809
Size: 507 3014 1802
Fragments arranged by size:
3014 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2770 3659 5288
Size: 54 1425 50 275 18 940 889 1629
Cuts at: 5288 8
Size: 43
Fragments arranged by size:
1629 1425 940 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2770 1537
Size: 275 958 4090
Fragments arranged by size:
4090 958 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2285 2580 3253 1353
Size: 891 41 295 673 3423
Fragments arranged by size:
3423 891 673 295 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 4284 4430 387
Size: 1064 138 2321 374 146 1280
Fragments arranged by size:
2321 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2585 3386 3983 4770 5038 1239
Size: 393 533 420 801 597 787 268 1524
Fragments arranged by size:
1524 801 787 597 533 420 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2147 2147
Size: 5323
EcoO109I rG'GnC_Cy
Cuts at: 2356 2356
Size: 5323
EcoRI G'AATT_C
Cuts at: 2632 3010 2632
Size: 378 4945
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 5323
FspI TGC'GCA
Cuts at: 3829 3829
Size: 5323
HaeII r_GCGC'y
Cuts at: 1678 2244 4470 1678
Size: 566 2226 2531
Fragments arranged by size:
2531 2226 566
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2442 2474 3964
Size: 32 135 32 427 32 411 32 1490
Cuts at: 3964 3996 4038 4070 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1490 427 411 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2667 2913 669
Size: 988 281 729 246 3079
Fragments arranged by size:
3079 988 729 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5323
HpaI GTT'AAC
Cuts at: 2913 2913
Size: 5323
KasI G'GCGC_C
Cuts at: 2240 2240
Size: 5323
KpnI G_GTAC'C
Cuts at: 2086 2648 2086
Size: 562 4761
MfeI C'AATT_G
Cuts at: 2922 2922
Size: 5323
MluI A'CGCG_T
Cuts at: 5199 5199
Size: 5323
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4437 4621 1305
Size: 3132 184 2007
Fragments arranged by size:
3132 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3501
Fragments arranged by size:
3501 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2462 3281 3640 3799 518
Size: 648 1296 819 359 159 2042
Fragments arranged by size:
2042 1296 819 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2172 2343 2739 3416 4564 4809 1606
Size: 531 35 171 396 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 396 245 171 35
NarI GG'CG_CC
Cuts at: 2241 2241
Size: 5323
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4771
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5323
NotI GC'GGCC_GC
Cuts at: 2770 2770
Size: 5323
NspI r_CATG'y
Cuts at: 1132 1229 2075 2681 4226 1132
Size: 97 846 606 1545 2229
Fragments arranged by size:
2229 1545 846 606 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5323
PciI A'CATG_T
Cuts at: 4222 4222
Size: 5323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5323
PmeI GTTT'AAAC
Cuts at: 2627 2627
Size: 5323
PmlI CAC'GTG
Cuts at: 2581 2581
Size: 5323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 4905 4937 3392
Size: 32 1481 32 3778
Fragments arranged by size:
3778 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5323
PsiI TTA'TAA
Cuts at: 2893 2893
Size: 5323
PstI C_TGCA'G
Cuts at: 830 2115 2348 2675 830
Size: 1285 233 327 3478
Fragments arranged by size:
3478 1285 327 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5323
PvuII CAG'CTG
Cuts at: 2172 2343 2172
Size: 171 5152
SalI G'TCGA_C
Cuts at: 1936 2665 1936
Size: 729 4594
SbfI CC_TGCA'GG
Cuts at: 2675 2675
Size: 5323
ScaI AGT'ACT
Cuts at: 3571 5283 3571
Size: 1712 3611
SfcI C'TryA_G
Cuts at: 826 1045 2111 2344 2671 3806 4487 4678 826
Size: 219 1066 233 327 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 681 327 233 219 191
SfoI GGC'GCC
Cuts at: 2242 2242
Size: 5323
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5323
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5323
SmaI CCC'GGG
Cuts at: 1872 2650 1872
Size: 778 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3427 4328 4590 820
Size: 197 926 1484 901 262 1553
Fragments arranged by size:
1553 1484 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3114 493
Size: 2621 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5323
SphI G_CATG'C
Cuts at: 1132 2681 1132
Size: 1549 3774
SspI AAT'ATT
Cuts at: 5 52 3247 5
Size: 47 3195 2081
Fragments arranged by size:
3195 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5323
StyI C'CwwG_G
Cuts at: 513 1065 2307 2359 2762 513
Size: 552 1242 52 403 3074
Fragments arranged by size:
3074 1242 552 403 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3367 3520 3705 4150 325
Size: 1255 1770 17 153 185 445 1498
Fragments arranged by size:
1770 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3569 5281 96
Size: 274 80 33 51 1443 1592 1712 138
Fragments arranged by size:
1712 1592 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5283 5315 5283
Size: 32 5291
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5323
XbaI T'CTAG_A
Cuts at: 2293 2659 2293
Size: 366 4957
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5323
XmaI C'CCGG_G
Cuts at: 1870 2648 1870
Size: 778 4545
XmnI GAAnn'nnTTC
Cuts at: 2279 3452 2279
Size: 1173 4150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3131 276
Size: 53 83 186 936 1597 2468
Fragments arranged by size:
2468 1597 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI