Restriction map for
pFN21AE0416
(Circular) (Six-base) MAPSORT of: pFN21AE0416.seq Check: 5640 from: 1 to: 5761
pFN21AE0416 5761 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2450 2591 2918 3824 5208 5476 1632
Size: 644 174 141 327 906 1384 268 1917
Fragments arranged by size:
1917 1384 906 644 327 268 174 141
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 2756 4660 5637 1203
Size: 1553 1904 977 1327
Fragments arranged by size:
1904 1553 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2060 3038 1166
Size: 894 978 3889
Fragments arranged by size:
3889 978 894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2284 2721 2794 2863 3504 5076 2284
Size: 437 73 69 641 1572 2969
Fragments arranged by size:
2969 1572 641 437 73 69
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 3036 3086 1870
Size: 73 160 933 50 4545
Fragments arranged by size:
4545 933 160 73 50
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3035 3068 3035
Size: 33 5728
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2389 3082 4537 618
Size: 325 217 627 264 338 693 1455 1842
Fragments arranged by size:
1842 1455 693 627 338 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2121 3003 3080 1391
Size: 730 882 77 4072
Fragments arranged by size:
4072 882 730 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 2864 3178 2864
Size: 314 5447
Bme1580I G_kGCm'C
Cuts at: 1790 2054 2379 2394 2961 3014 3822 4978 1790
Size: 264 325 15 567 53 808 1156 2573
Fragments arranged by size:
2573 1156 808 567 325 264 53 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 2969 4421 1239
Size: 924 806 1452 2579
Fragments arranged by size:
2579 1452 924 806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2105 2804 3886 4751 5049 2105
Size: 699 1082 865 298 2817
Fragments arranged by size:
2817 1082 865 699 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2113 2549 4424 882
Size: 617 614 436 1875 2219
Fragments arranged by size:
2219 1875 617 614 436
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3568 3950 4585 275
Size: 53 83 186 936 2035 382 635 1451
Fragments arranged by size:
2035 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2781 4194 4592 4866 5013 5424 2781
Size: 1413 398 274 147 411 3118
Fragments arranged by size:
3118 1413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2576 2849 1186
Size: 1390 273 4098
Fragments arranged by size:
4098 1390 273
BseYI C'CCAG_C
Cuts at: 2856 4964 5460 2856
Size: 2108 496 3157
Fragments arranged by size:
3157 2108 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2956 1163
Size: 573 1220 3968
Fragments arranged by size:
3968 1220 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2301 3822 3907 4978 2121
Size: 180 1521 85 1071 2904
Fragments arranged by size:
2904 1521 1071 180 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2121 2301 2379 2394 2961 3003
Size: 399 264 67 180 78 15 567 42
Cuts at: 3003 3014 3080 3822 3907 4978 1391
Size: 11 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 264 180 85
78 67 66 42 15 11
BspEI T'CCGG_A
Cuts at: 2781 5424 2781
Size: 2643 3118
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 844
Size: 2272 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2536 3648 1394
Size: 429 190 523 1112 3507
Fragments arranged by size:
3507 1112 523 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 4405 4592 1700
Size: 110 281 2314 187 2869
Fragments arranged by size:
2869 2314 281 187 110
BsrGI T'GTAC_A
Cuts at: 96 3051 96
Size: 2955 2806
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 2396 3821 4833 2396
Size: 1425 1012 3324
Fragments arranged by size:
3324 1425 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2794 2979 794
Size: 2000 185 3576
Fragments arranged by size:
3576 2000 185
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 2065 1184
Size: 881 4880
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2402 2062
Size: 340 5421
BstYI r'GATC_y
Cuts at: 1972 2449 3091 3458 3843 3860 5312 5398 5410
Size: 477 642 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 642 477 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2880 2880
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 4071 4091 3277
Size: 794 20 4947
Fragments arranged by size:
4947 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 2157 3065 2157
Size: 908 4853
DraIII CAC_nnn'GTG
Cuts at: 2979 2979
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2515 3208 4097
Size: 54 1425 50 275 18 685 693 889
Cuts at: 4097 5726 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 693 685 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2774 2894 3691 1353
Size: 1421 120 797 3423
Fragments arranged by size:
3423 1421 797 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2618 4348 4722 4868 387
Size: 1064 138 1029 1730 374 146 1280
Fragments arranged by size:
1730 1280 1064 1029 374 146 138
EcoICRI GAG'CTC
Cuts at: 2119 2119
Size: 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2276 2450 2591 2918 2969 3824
Size: 393 531 113 174 141 327 51 855
Cuts at: 3824 4421 5208 5476 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 855 787 597 531 393 327 268
174 141 113 51
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2738 2812 2907 4908 1678
Size: 1060 74 95 2001 2531
Fragments arranged by size:
2531 2001 1060 95 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2341 2373 2606 2638 3015
Size: 32 135 32 769 32 233 32 377
Cuts at: 3015 3047 4402 4434 4476 4508 1373
Size: 32 1355 32 42 32 2626
Fragments arranged by size:
2626 1355 769 377 233 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4875 5059 1305
Size: 3570 184 2007
Fragments arranged by size:
3570 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3939
Fragments arranged by size:
3939 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2145 2400 3038 3719 4078 4237
Size: 648 894 85 255 638 681 359 159
Cuts at: 4237 518
Size: 2042
Fragments arranged by size:
2042 894 681 648 638 359 255 159
85
MspA1I CmG'CkG
Cuts at: 1606 2281 2529 2800 2860 3177 3854 5002 5247
Size: 675 248 271 60 317 677 1148 245
Cuts at: 5247 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 675 317 271 248 245
60
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2656 2760 3119 4664 1132
Size: 97 1427 104 359 1545 2229
Fragments arranged by size:
2229 1545 1427 359 104 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 2756 4660 2756
Size: 1904 3857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2254 1108
Size: 1146 4615
PfoI T'CCnGG_A
Cuts at: 2179 2610 2841 2179
Size: 431 231 5099
Fragments arranged by size:
5099 431 231
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2462 2494 2462
Size: 32 5729
PstI C_TGCA'G
Cuts at: 830 2637 2697 2799 3023 3113 830
Size: 1807 60 102 224 90 3478
Fragments arranged by size:
3478 1807 224 102 90 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2860 2860
Size: 5761
SacI G_AGCT'C
Cuts at: 2121 2121
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SapI GCTCTTCn'nnn_
Cuts at: 2894 2894
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 2660 4009 5721 2660
Size: 1349 1712 2700
Fragments arranged by size:
2700 1712 1349
SexAI A'CCwGG_T
Cuts at: 2385 2385
Size: 5761
SfcI C'TryA_G
Cuts at: 826 1045 2633 2693 2795 3019 3109 4244 4925
Size: 219 1588 60 102 224 90 1135 681
Cuts at: 4925 5116 826
Size: 191 1471
Fragments arranged by size:
1588 1471 1135 681 224 219 191 102
90 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2105 3038 3088 1872
Size: 233 933 50 4545
Fragments arranged by size:
4545 933 233 50
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 2819 3865 4766 5028 820
Size: 197 926 177 699 1046 901 262 1553
Fragments arranged by size:
1553 1046 926 901 699 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 2656 3119 1132
Size: 1524 463 3774
Fragments arranged by size:
3774 1524 463
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5761
StyI C'CwwG_G
Cuts at: 513 1065 2004 3200 513
Size: 552 939 1196 3074
Fragments arranged by size:
3074 1196 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 2339 2573 3788 3805 3958 4143
Size: 1255 555 204 234 1215 17 153 185
Cuts at: 4143 4588 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1215 555 445 234 204 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2079 2658 3051
Size: 274 80 33 51 1443 102 579 393
Cuts at: 3051 4007 5719 96
Size: 956 1712 138
Fragments arranged by size:
1712 1443 956 579 393 274 138 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2175 2207 2951 2983 5721 5753 2175
Size: 32 744 32 2738 32 2183
Fragments arranged by size:
2738 2183 744 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 2458 3097 2458
Size: 639 5122
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2103 3036 3086 1870
Size: 233 933 50 4545
Fragments arranged by size:
4545 933 233 50
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI