Restriction map for
pFN21AE0413
(Circular) (Six-base) MAPSORT of: pFN21AE0413.seq Check: 6826 from: 1 to: 5707
pFN21AE0413 5707 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2830 820
Size: 197 1813 3697
Fragments arranged by size:
3697 1813 197
AflIII A'CryG_T
Cuts at: 1203 2671 4606 5583 1203
Size: 1468 1935 977 1327
Fragments arranged by size:
1935 1468 1327 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 2996 4436 2996
Size: 1440 4267
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2327 2381 2597 3450 5022 2327
Size: 54 216 853 1572 3012
Fragments arranged by size:
3012 1572 853 216 54
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2144 2201 2359 2473 3016 3242 3394 5503
Size: 651 57 158 114 543 226 152 2109
Cuts at: 5503 1493
Size: 1697
Fragments arranged by size:
2109 1697 651 543 226 158 152 114
57
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 3032 1870
Size: 73 102 987 4545
Fragments arranged by size:
4545 987 102 73
AvrII C'CTAG_G
Cuts at: 2857 2857
Size: 5707
BamHI G'GATC_C
Cuts at: 2725 3037 3404 2725
Size: 312 367 5028
Fragments arranged by size:
5028 367 312
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 3028 4483 618
Size: 325 217 627 321 920 1455 1842
Fragments arranged by size:
1842 1455 920 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2028 3026 1391
Size: 637 998 4072
Fragments arranged by size:
4072 998 637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 2460 928
Size: 971 403 158 4175
Fragments arranged by size:
4175 971 403 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2570 2604 3898 3932 1903
Size: 34 633 34 1294 34 3678
Fragments arranged by size:
3678 1294 633 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2883 3124 2883
Size: 241 5466
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2124 3438 4396
Size: 416 53 83 399 24 707 1314 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1314 958 707 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2708 1056
Size: 1652 4055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2411 2898 2411
Size: 487 5220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 4367 1239
Size: 1298 1830 2579
Fragments arranged by size:
2579 1830 1298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2900 4370 882
Size: 617 1401 1470 2219
Fragments arranged by size:
2219 1470 1401 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2523 2553 1178
Size: 30 165 30 1120 30 4332
Fragments arranged by size:
4332 1120 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 1186
Size: 895 4812
BseYI C'CCAG_C
Cuts at: 2819 4910 5406 2819
Size: 2091 496 3120
Fragments arranged by size:
3120 2091 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2304 2610 2636 1163
Size: 573 568 306 26 4234
Fragments arranged by size:
4234 573 568 306 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 2866 3768 3853 4924 2028
Size: 838 902 85 1071 2811
Fragments arranged by size:
2811 1071 902 838 85
BsmI GAATG_Cn'
Cuts at: 2724 3218 3311 2724
Size: 494 93 5120
Fragments arranged by size:
5120 494 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2866 3026 3768 3853 4924 1391
Size: 399 238 838 160 742 85 1071 2174
Fragments arranged by size:
2174 1071 838 742 399 238 160 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 2251 3596 5326 2251
Size: 1345 1730 2632
Fragments arranged by size:
2632 1730 1345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2767 4202 4376 65
Size: 1183 1519 1435 174 1396
Fragments arranged by size:
1519 1435 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 4351 4538 1700
Size: 110 887 1654 187 2869
Fragments arranged by size:
2869 1654 887 187 110
BsrGI T'GTAC_A
Cuts at: 96 2134 96
Size: 2038 3669
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 2938 4511 2938
Size: 1573 4134
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2725 3037 3404 3789 3806 5258 5344 5356
Size: 753 312 367 385 17 1452 86 12
Cuts at: 5356 5702 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 753 385 367 346 312 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2598 3223 4017 4037 2598
Size: 625 794 20 4268
Fragments arranged by size:
4268 794 625 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2694 2694
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2852 4714 809
Size: 507 1536 1862 1802
Fragments arranged by size:
1862 1802 1536 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2685 2920 3154
Size: 54 1425 50 275 18 855 235 234
Cuts at: 3154 4043 5672 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 855 275 235 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2708 3637 1353
Size: 829 526 929 3423
Fragments arranged by size:
3423 929 829 526
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2026
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 3770 4367 5154 5422 1239
Size: 393 905 1233 597 787 268 1524
Fragments arranged by size:
1524 1233 905 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2856 2856
Size: 5707
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2975 2761
Size: 214 5493
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2982 3014 4348
Size: 32 135 32 743 32 635 32 1334
Cuts at: 4348 4380 4422 4454 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1334 743 635 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 3051 3297 669
Size: 988 281 139 974 246 3079
Fragments arranged by size:
3079 988 974 281 246 139
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 2077 3297 2077
Size: 1220 4487
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 2796 3306 2796
Size: 510 5197
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 2365 4821 5005 1305
Size: 897 163 2456 184 2007
Fragments arranged by size:
2456 2007 897 184 163
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2687 2922 10
Size: 54 1425 343 855 235 2795
Fragments arranged by size:
2795 1425 855 343 235 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3665 4024 4183 518
Size: 648 2499 359 159 2042
Fragments arranged by size:
2499 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2378 3123 3800 4948 5193 1606
Size: 772 745 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 745 677 245
NaeI GCC'GGC
Cuts at: 2699 2699
Size: 5707
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NgoMIV G'CCGG_C
Cuts at: 2697 2697
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 2704 1052
Size: 1652 4055
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2168 2675 2793 3065 4610 1132
Size: 97 939 507 118 272 1545 2229
Fragments arranged by size:
2229 1545 939 507 272 118 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 2671 4606 2671
Size: 1935 3772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PfoI T'CCnGG_A
Cuts at: 2514 2514
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2378 2378
Size: 5707
SacI G_AGCT'C
Cuts at: 2028 2028
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SapI GCTCTTCn'nnn_
Cuts at: 2182 2182
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 2047 3034 1872
Size: 175 987 4545
Fragments arranged by size:
4545 987 175
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2830 3811 4712 4974 820
Size: 197 926 887 981 901 262 1553
Fragments arranged by size:
1553 981 926 901 887 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 2168 3065 1132
Size: 1036 897 3774
Fragments arranged by size:
3774 1036 897
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 2857 2910 3146 513
Size: 552 939 853 53 236 3074
Fragments arranged by size:
3074 939 853 552 236 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2134 2275 3953
Size: 274 80 33 51 1443 157 141 1678
Cuts at: 3953 5665 96
Size: 1712 138
Fragments arranged by size:
1712 1678 1443 274 157 141 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 2045 3032 1870
Size: 175 987 4545
Fragments arranged by size:
4545 987 175
XmnI GAAnn'nnTTC
Cuts at: 3836 3836
Size: 5707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI