Restriction map for
pFN21AE0412
(Circular) (Six-base) MAPSORT of: pFN21AE0412.seq Check: 1833 from: 1 to: 5434
pFN21AE0412 5434 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2664 2755 2664
Size: 91 5343
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3497 4881 5149 1632
Size: 1865 1384 268 1917
Fragments arranged by size:
1917 1865 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 2348 4333 5310 1203
Size: 1145 1985 977 1327
Fragments arranged by size:
1985 1327 1145 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 3177 4749 3177
Size: 1572 3862
ApaI G_GGCC'C
Cuts at: 2051 2076 2110 2051
Size: 25 34 5375
Fragments arranged by size:
5375 34 25
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 1870
Size: 73 816 4545
Fragments arranged by size:
4545 816 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2648 2681 2648
Size: 33 5401
BamHI G'GATC_C
Cuts at: 2764 3131 2764
Size: 367 5067
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2664 2755 4210 618
Size: 325 217 627 877 91 1455 1842
Fragments arranged by size:
1842 1455 877 627 325 217 91
BanII G_rGCy'C
Cuts at: 1391 2051 2076 2110 2136 2753 1391
Size: 660 25 34 26 617 4072
Fragments arranged by size:
4072 660 617 34 26 25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2665 928
Size: 971 766 3697
Fragments arranged by size:
3697 971 766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4045 136
Size: 107 122 71 631 605 2373 1525
Fragments arranged by size:
2373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2519 2851 2519
Size: 332 5102
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2076 2102 2110 2395 3495 4651 1790
Size: 261 25 26 8 285 1100 1156 2573
Fragments arranged by size:
2573 1156 1100 285 261 26 25 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3165 4123 442
Size: 416 53 83 399 24 1748 958 1753
Fragments arranged by size:
1753 1748 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4094 1239
Size: 2855 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 4097 882
Size: 617 966 1632 2219
Fragments arranged by size:
2219 1632 966 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3241 3623 4258
Size: 53 83 186 936 531 1177 382 635
Cuts at: 4258 275
Size: 1451
Fragments arranged by size:
1451 1177 936 635 531 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3867 4265 4539 4686 5097 3867
Size: 398 274 147 411 4204
Fragments arranged by size:
4204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2144 2230 2497 1186
Size: 955 3 86 267 4123
Fragments arranged by size:
4123 955 267 86 3
BseYI C'CCAG_C
Cuts at: 2069 2273 2721 4637 5133 2069
Size: 204 448 1916 496 2370
Fragments arranged by size:
2370 1916 496 448 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4861
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2884 3645 4673 1540
Size: 275 186 57 826 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 3495 3580 4651 3495
Size: 85 1071 4278
Fragments arranged by size:
4278 1071 85
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2076 2102 2110 2136 2395 2753
Size: 399 261 25 26 8 26 259 358
Cuts at: 2753 3495 3580 4651 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 261 259 85
26 26 25 8
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2150 2789 844
Size: 1306 639 3489
Fragments arranged by size:
3489 1306 639
BsrBI CCG'CTC
Cuts at: 1394 1823 2518 3321 1394
Size: 429 695 803 3507
Fragments arranged by size:
3507 803 695 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2053 2154 4078 4265 1700
Size: 110 243 101 1924 187 2869
Fragments arranged by size:
2869 1924 243 187 110 101
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2764 3131 3516 3533 4985 5071 5083 5429
Size: 792 367 385 17 1452 86 12 346
Cuts at: 5429 1972
Size: 1977
Fragments arranged by size:
1977 1452 792 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2208 2421 513
Size: 552 939 54 150 213 3526
Fragments arranged by size:
3526 939 552 213 150 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2182 487
Size: 683 56 956 3739
Fragments arranged by size:
3739 956 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2055 2418
Size: 54 1425 50 275 18 199 26 363
Cuts at: 2418 2881 3770 5399 8
Size: 463 889 1629 43
Fragments arranged by size:
1629 1425 889 463 363 275 199 54
50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2881 1537
Size: 275 243 826 4090
Fragments arranged by size:
4090 826 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 3364 1353
Size: 2011 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4395 4541 387
Size: 1064 138 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3497 4094 4881 5149 1239
Size: 393 1865 597 787 268 1524
Fragments arranged by size:
1865 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2047 2106 2107 2526 2717 2047
Size: 59 1 419 191 4764
Fragments arranged by size:
4764 419 191 59 1
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 4581 1678
Size: 2903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2338 2778 3024 669
Size: 988 281 400 440 246 3079
Fragments arranged by size:
3079 988 440 400 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2668 2759 2668
Size: 91 5343
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 4732 1305
Size: 3243 184 2007
Fragments arranged by size:
3243 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3612
Fragments arranged by size:
3612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2597 3392 3751 3910 518
Size: 648 1035 396 795 359 159 2042
Fragments arranged by size:
2042 1035 795 648 396 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2069 2164 2185 2850 3527 4675 4920
Size: 454 9 95 21 665 677 1148 245
Cuts at: 4920 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 665 454 245 95 21
9
NaeI GCC'GGC
Cuts at: 2055 2055
Size: 5434
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3943
Fragments arranged by size:
3943 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NgoMIV G'CCGG_C
Cuts at: 2053 2053
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2352 2792 4337 1132
Size: 97 1123 440 1545 2229
Fragments arranged by size:
2229 1545 1123 440 97
PasI CC'CwG_GG
Cuts at: 1348 2191 2395 1348
Size: 843 204 4387
Fragments arranged by size:
4387 843 204
PciI A'CATG_T
Cuts at: 2348 4333 2348
Size: 1985 3449
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2526 2526
Size: 5434
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PspOMI G'GGCC_C
Cuts at: 2047 2072 2106 2047
Size: 25 34 5375
Fragments arranged by size:
5375 34 25
PstI C_TGCA'G
Cuts at: 830 2184 2786 830
Size: 1354 602 3478
Fragments arranged by size:
3478 1354 602
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 5434
SacII CC_GC'GG
Cuts at: 2061 2061
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SexAI A'CCwGG_T
Cuts at: 2667 2667
Size: 5434
SfcI C'TryA_G
Cuts at: 826 1045 2180 2782 3917 4598 4789 826
Size: 219 1135 602 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 681 602 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3538 4439 4701 820
Size: 197 926 1595 901 262 1553
Fragments arranged by size:
1595 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2873 513
Size: 552 939 108 761 3074
Fragments arranged by size:
3074 939 761 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 3461 3478 3631 3816 4261 325
Size: 1255 1092 789 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1092 789 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI