Restriction map for
pFN21AE0408
(Circular) (Six-base) MAPSORT of: pFN21AE0408.seq Check: 4540 from: 1 to: 5692
pFN21AE0408 5692 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2215 2215
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2652 3035 1937
Size: 715 383 4594
Fragments arranged by size:
4594 715 383
Acc65I G'GTAC_C
Cuts at: 2828 3013 2828
Size: 185 5507
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2118 2184 3755 5139 5407 1632
Size: 423 63 66 1571 1384 268 1917
Fragments arranged by size:
1917 1571 1384 423 268 66 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2243 3435 5007 2243
Size: 1192 1572 2928
Fragments arranged by size:
2928 1572 1192
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2253 3001 3227 3379 5488 1493
Size: 760 748 226 152 2109 1697
Fragments arranged by size:
2109 1697 760 748 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 2828 3013 4468 618
Size: 325 217 627 440 601 185 1455 1842
Fragments arranged by size:
1842 1455 627 601 440 325 217 185
BanII G_rGCy'C
Cuts at: 1391 2022 2502 2697 3011 1391
Size: 631 480 195 314 4072
Fragments arranged by size:
4072 631 480 314 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BbvCI CC'TCA_GC
Cuts at: 2106 2106
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2812 3584 4794 2812
Size: 772 1210 3710
Fragments arranged by size:
3710 1210 772
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2232 2720 3753 4909 1790
Size: 338 104 488 1033 1156 2573
Fragments arranged by size:
2573 1156 1033 488 338 104
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2671 3423 4381
Size: 416 53 83 399 24 1254 752 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1254 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2077 2548 2077
Size: 471 5221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2543 2891 2972 4352 1239
Size: 1115 189 348 81 1380 2579
Fragments arranged by size:
2579 1380 1115 348 189 81
Bpu10I CC'TnA_GC
Cuts at: 2106 2106
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2593 4125 4523 4797 4944 5355 2593
Size: 1532 398 274 147 411 2930
Fragments arranged by size:
2930 1532 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5467
Fragments arranged by size:
5467 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2057 2060 2069 2777 2786 2789 2969
Size: 868 3 3 9 708 9 3 180
Cuts at: 2969 2972 1186
Size: 3 3906
Fragments arranged by size:
3906 868 708 180 9 9 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2021 2858 4895 5391 2021
Size: 837 2037 496 2322
Fragments arranged by size:
2322 2037 837 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 1163
Size: 573 894 4225
Fragments arranged by size:
4225 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2502 3753 3838 4909 2061
Size: 441 1251 85 1071 2844
Fragments arranged by size:
2844 1251 1071 441 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
BsmBI CGTCTCn'nnnn_
Cuts at: 2519 2519
Size: 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2061 2128 2232 2502 2697 2720
Size: 399 232 39 67 104 270 195 23
Cuts at: 2720 3011 3753 3838 4909 1391
Size: 291 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 270 232 195
104 85 67 39 23
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 3047 844
Size: 1371 832 3489
Fragments arranged by size:
3489 1371 832
BsrBI CCG'CTC
Cuts at: 1394 1823 3579 1394
Size: 429 1756 3507
Fragments arranged by size:
3507 1756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2207 4336 4523 1700
Size: 110 397 2129 187 2869
Fragments arranged by size:
2869 2129 397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 2429 3752 4764 2429
Size: 1323 1012 3357
Fragments arranged by size:
3357 1323 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5692
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2244 2244
Size: 5692
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 3208 4002 4022 2321
Size: 887 794 20 3991
Fragments arranged by size:
3991 887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657
Size: 54 1425 50 275 18 1309 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 1309 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2046 3622 1353
Size: 678 15 1576 3423
Fragments arranged by size:
3423 1576 678 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2500 2500
Size: 5692
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2118 2184 2354 2543 2891 2972
Size: 393 423 63 66 170 189 348 81
Cuts at: 2972 3755 4352 5139 5407 1239
Size: 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 423 393 348 268
189 170 81 66 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 2951 2309
Size: 642 5050
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2303 2335 4333 4365 4407
Size: 32 135 32 731 32 1998 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1998 731 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3036 3282 669
Size: 988 281 334 764 246 3079
Fragments arranged by size:
3079 988 764 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 2832 3017 2832
Size: 185 5507
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 2765 4806 4990 1305
Size: 1206 254 2041 184 2007
Fragments arranged by size:
2041 2007 1206 254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3650 4009 4168 518
Size: 648 843 1641 359 159 2042
Fragments arranged by size:
2042 1641 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2917 3108 3785 4933 5178 1606
Size: 1311 191 677 1148 245 2120
Fragments arranged by size:
2120 1311 1148 677 245 191
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4201
Fragments arranged by size:
4201 939 552
NdeI CA'TA_TG
Cuts at: 387 2629 387
Size: 2242 3450
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 3050 4595 1132
Size: 97 1821 1545 2229
Fragments arranged by size:
2229 1821 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5692
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2916 3044 830
Size: 2086 128 3478
Fragments arranged by size:
3478 2086 128
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SacI G_AGCT'C
Cuts at: 2502 2502
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2503 2653 2912 2941 3040 4175 4856
Size: 219 1458 150 259 29 99 1135 681
Cuts at: 4856 5047 826
Size: 191 1471
Fragments arranged by size:
1471 1458 1135 681 259 219 191 150
99 29
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3796 4697 4959 820
Size: 197 926 1853 901 262 1553
Fragments arranged by size:
1853 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2264 2823 3131 513
Size: 552 939 260 559 308 3074
Fragments arranged by size:
3074 939 559 552 308 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2548
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 2708 3821 2708
Size: 1113 4579
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI