Restriction map for
pFN21AE0405
(Circular) (Six-base) MAPSORT of: pFN21AE0405.seq Check: 271 from: 1 to: 5599
pFN21AE0405 5599 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 2316 2710 2942 1937
Size: 84 295 394 232 4594
Fragments arranged by size:
4594 394 295 232 84
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2711 3662 5046 5314 1632
Size: 1079 951 1384 268 1917
Fragments arranged by size:
1917 1384 1079 951 268
AflII C'TTAA_G
Cuts at: 820 1017 2437 820
Size: 197 1420 3982
Fragments arranged by size:
3982 1420 197
AflIII A'CryG_T
Cuts at: 1203 2282 2713 4498 5475 1203
Size: 1079 431 1785 977 1327
Fragments arranged by size:
1785 1327 1079 977 431
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2461 2908 3134 3286 5395 1493
Size: 968 447 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 447 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2920 4375 618
Size: 325 217 627 1133 1455 1842
Fragments arranged by size:
1842 1455 1133 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2918 1391
Size: 1527 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 2207 2449 928
Size: 971 116 192 242 4078
Fragments arranged by size:
4078 971 242 192 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2815 2849 3790 3824 1903
Size: 34 878 34 941 34 3678
Fragments arranged by size:
3678 941 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3491 4701 3491
Size: 1210 4389
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5599
BfrBI ATG'CAT
Cuts at: 2627 2627
Size: 5599
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2823 4210 136
Size: 107 122 71 631 605 1151 1387 1525
Fragments arranged by size:
1525 1387 1151 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 3660 4816 1790
Size: 1870 1156 2573
Fragments arranged by size:
2573 1870 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4288 442
Size: 416 53 83 399 24 1913 958 1753
Fragments arranged by size:
1913 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2840 4259 1239
Size: 1601 1419 2579
Fragments arranged by size:
2579 1601 1419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4589 4887 3724
Size: 865 298 4436
Fragments arranged by size:
4436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2283 2714 3390 493
Size: 711 1079 431 676 2702
Fragments arranged by size:
2702 1079 711 676 431
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 3788 4423 275
Size: 53 83 186 936 1873 382 635 1451
Fragments arranged by size:
1873 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5374
Fragments arranged by size:
5374 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5599
BseYI C'CCAG_C
Cuts at: 2073 4802 5298 2073
Size: 2729 496 2374
Fragments arranged by size:
2729 2374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5026
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 3745 4816 3660
Size: 85 1071 4443
Fragments arranged by size:
4443 1071 85
BsmI GAATG_Cn'
Cuts at: 2627 3110 3203 2627
Size: 483 93 5023
Fragments arranged by size:
5023 483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2918 3660 3745 4816 1391
Size: 399 1128 742 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2954 844
Size: 2110 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstYI r'GATC_y
Cuts at: 1972 2929 3296 3681 3698 5150 5236 5248 5594
Size: 957 367 385 17 1452 86 12 346
Cuts at: 5594 1972
Size: 1977
Fragments arranged by size:
1977 1452 957 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2317 2317
Size: 5599
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 3115 3909 3929 3115
Size: 794 20 4785
Fragments arranged by size:
4785 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2017 4606 809
Size: 507 701 2589 1802
Fragments arranged by size:
2589 1802 701 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3935 5564
Size: 54 1425 50 275 18 1216 889 1629
Cuts at: 5564 8
Size: 43
Fragments arranged by size:
1629 1425 1216 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2802 3529 1353
Size: 1449 727 3423
Fragments arranged by size:
3423 1449 727
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2711 2840 3662 4259 5046 5314 1239
Size: 393 1079 129 822 597 787 268 1524
Fragments arranged by size:
1524 1079 822 787 597 393 268 129
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 4746 1678
Size: 3068 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4240 4272 4314 4346 1373
Size: 32 135 32 2668 32 42 32 2626
Fragments arranged by size:
2668 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 2943 3189 669
Size: 988 281 394 611 246 3079
Fragments arranged by size:
3079 988 611 394 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 4897 1305
Size: 3408 184 2007
Fragments arranged by size:
3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3777
Fragments arranged by size:
3777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3557 3916 4075 518
Size: 648 2391 359 159 2042
Fragments arranged by size:
2391 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3015 3692 4840 5085 1606
Size: 1409 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5047
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NsiI A_TGCA'T
Cuts at: 2629 2629
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2957 4502 1132
Size: 97 1728 1545 2229
Fragments arranged by size:
2229 1728 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PfoI T'CCnGG_A
Cuts at: 2793 2793
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PmlI CAC'GTG
Cuts at: 2283 2283
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2019 2951 830
Size: 1189 932 3478
Fragments arranged by size:
3478 1189 932
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SfcI C'TryA_G
Cuts at: 826 1045 2015 2947 4082 4763 4954 826
Size: 219 970 932 1135 681 191 1471
Fragments arranged by size:
1471 1135 970 932 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2437 3703 4604 4866 820
Size: 197 926 494 1266 901 262 1553
Fragments arranged by size:
1553 1266 926 901 494 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 2173 3523 5
Size: 47 2121 1350 2081
Fragments arranged by size:
2121 2081 1350 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5599
StyI C'CwwG_G
Cuts at: 513 1065 3038 513
Size: 552 1973 3074
Fragments arranged by size:
3074 1973 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3626 3643 3796 3981 4426 325
Size: 1255 2046 17 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 3728 3728
Size: 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI