Restriction map for
pFN21AE0402
(Circular) (Six-base) MAPSORT of: pFN21AE0402.seq Check: 3100 from: 1 to: 5617
pFN21AE0402 5617 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2757 2960 1937
Size: 820 203 4594
Fragments arranged by size:
4594 820 203
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 3744 4117 1563
Size: 2181 373 3063
Fragments arranged by size:
3063 2181 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2862 2896 3680 5064 5332 1632
Size: 689 541 34 784 1384 268 1917
Fragments arranged by size:
1917 1384 784 689 541 268 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2544 4516 5493 1203
Size: 1341 1972 977 1327
Fragments arranged by size:
1972 1341 1327 977
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5617
AlwNI CAG_nnn'CTG
Cuts at: 2228 3360 4932 2228
Size: 1132 1572 2913
Fragments arranged by size:
2913 1572 1132
ApaLI G'TGCA_C
Cuts at: 2597 3674 4830 2597
Size: 1077 1156 3384
Fragments arranged by size:
3384 1156 1077
ApoI r'AATT_y
Cuts at: 1493 2464 2807 2926 3152 3304 5413 1493
Size: 971 343 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 971 343 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2942 1870
Size: 73 999 4545
Fragments arranged by size:
4545 999 73
BamHI G'GATC_C
Cuts at: 2769 2947 3314 2769
Size: 178 367 5072
Fragments arranged by size:
5072 367 178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2938 4393 618
Size: 325 217 627 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2179 2936 1391
Size: 788 757 4072
Fragments arranged by size:
4072 788 757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2066 928
Size: 971 167 4479
Fragments arranged by size:
4479 971 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3808 3842 1903
Size: 34 1871 34 3678
Fragments arranged by size:
3678 1871 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BfrBI ATG'CAT
Cuts at: 2348 2348
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 3034 3034
Size: 5617
Bme1580I G_kGCm'C
Cuts at: 1790 2601 3678 4834 1790
Size: 811 1077 1156 2573
Fragments arranged by size:
2573 1156 1077 811
BmgBI CAC'GTC
Cuts at: 1318 2366 1318
Size: 1048 4569
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2910 4277 1239
Size: 1671 1367 2579
Fragments arranged by size:
2579 1671 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3742 4607 4905 3742
Size: 865 298 4454
Fragments arranged by size:
4454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2547 3408 493
Size: 711 1343 861 2702
Fragments arranged by size:
2702 1343 861 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4050 4448 4722 4869 5280 4050
Size: 398 274 147 411 4387
Fragments arranged by size:
4387 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5392
Fragments arranged by size:
5392 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5617
BseYI C'CCAG_C
Cuts at: 2326 4820 5316 2326
Size: 2494 496 2627
Fragments arranged by size:
2627 2494 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3067 3828 4856 1540
Size: 275 186 1066 761 1028 2301
Fragments arranged by size:
2301 1066 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 2601 3678 3763 4834 2445
Size: 156 1077 85 1071 3228
Fragments arranged by size:
3228 1077 1071 156 85
BsmI GAATG_Cn'
Cuts at: 2348 2855 3128 3221 2348
Size: 507 273 93 4744
Fragments arranged by size:
4744 507 273 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2445 2601 2936 3678 3763 4834
Size: 399 389 266 156 335 742 85 1071
Cuts at: 4834 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 389 335 266 156
85
BspEI T'CCGG_A
Cuts at: 5280 5280
Size: 5617
BspHI T'CATG_A
Cuts at: 3506 5236 3506
Size: 1730 3887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 1394
Size: 429 1681 3507
Fragments arranged by size:
3507 1681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4112 4286 65
Size: 1183 2864 174 1396
Fragments arranged by size:
2864 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4261 4448 1700
Size: 110 2451 187 2869
Fragments arranged by size:
2869 2451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5617
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2769 2947 3314 3699 3716 5168 5254 5266
Size: 797 178 367 385 17 1452 86 12
Cuts at: 5266 5612 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 797 385 367 346 178 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 513
Size: 552 939 684 3442
Fragments arranged by size:
3442 939 684 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 2026 3133 3927 3947 2026
Size: 1107 794 20 3696
Fragments arranged by size:
3696 1107 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2830 2921 2830
Size: 91 5526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2753 4624 809
Size: 507 1437 1871 1802
Fragments arranged by size:
1871 1802 1437 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3064 3953 5582
Size: 54 1425 50 275 18 1234 889 1629
Cuts at: 5582 8
Size: 43
Fragments arranged by size:
1629 1425 1234 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3064 1537
Size: 275 1252 4090
Fragments arranged by size:
4090 1252 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 2160 2252 2655 3547 1353
Size: 675 132 92 403 892 3423
Fragments arranged by size:
3423 892 675 403 132 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2321 2862 2896 2910 3680 4277 5064
Size: 393 689 541 34 14 770 597 787
Cuts at: 5064 5332 1239
Size: 268 1524
Fragments arranged by size:
1524 787 770 689 597 541 393 268
34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2184 2184
Size: 5617
EcoO109I rG'GnC_Cy
Cuts at: 2190 2359 2190
Size: 169 5448
EcoRI G'AATT_C
Cuts at: 2464 2807 2926 3304 2464
Size: 343 119 378 4777
Fragments arranged by size:
4777 378 343 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 5617
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 4258 4290 4332
Size: 32 135 32 542 32 2112 32 42
Cuts at: 4332 4364 1373
Size: 32 2626
Fragments arranged by size:
2626 2112 542 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2073 2457 2961 3207 669
Size: 988 281 135 384 504 246 3079
Fragments arranged by size:
3079 988 504 384 281 246 135
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 2457 3207 2457
Size: 750 4867
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 5493 5493
Size: 5617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2747 4731 4915 1305
Size: 1442 1984 184 2007
Fragments arranged by size:
2007 1984 1442 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 2726 3575 3934 4093 518
Size: 648 1467 93 849 359 159 2042
Fragments arranged by size:
2042 1467 849 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 2330 3033 3710 4858 5103 1606
Size: 724 703 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 703 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4126
Fragments arranged by size:
4126 939 552
NdeI CA'TA_TG
Cuts at: 387 2493 387
Size: 2106 3511
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NsiI A_TGCA'T
Cuts at: 2350 2350
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2975 4520 1132
Size: 97 1746 1545 2229
Fragments arranged by size:
2229 1746 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5617
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PmlI CAC'GTG
Cuts at: 2547 2547
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 5617
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2206 2969 830
Size: 1376 763 3478
Fragments arranged by size:
3478 1376 763
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
PvuII CAG'CTG
Cuts at: 2330 2330
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SapI GCTCTTCn'nnn_
Cuts at: 2160 2655 2160
Size: 495 5122
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2202 2965 4100 4781 4972 826
Size: 219 1157 763 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 763 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3721 4622 4884 820
Size: 197 926 1778 901 262 1553
Fragments arranged by size:
1778 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 2004 2389 3056 513
Size: 552 939 385 667 3074
Fragments arranged by size:
3074 939 667 552 385
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 3661 3814 3999 4444 325
Size: 1255 2064 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 2718 3863
Size: 274 80 33 51 1443 276 465 1145
Cuts at: 3863 5575 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1145 465 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5577 5609 5577
Size: 32 5585
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2743 2953 2743
Size: 210 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 2306 2855 3746 2306
Size: 549 891 4177
Fragments arranged by size:
4177 891 549
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI