Restriction map for
pFN21AE0394
(Circular) (Six-base) MAPSORT of: pFN21AE0394.seq Check: 9608 from: 1 to: 8065
pFN21AE0394 8065 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5875 278
Size: 53 83 186 936 4339 2468
Fragments arranged by size:
4339 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5408 1937
Size: 3471 4594
Acc65I G'GTAC_C
Cuts at: 3616 3733 5386 3616
Size: 117 1653 6295
Fragments arranged by size:
6295 1653 117
AclI AA'CG_TT
Cuts at: 1563 6192 6565 1563
Size: 4629 373 3063
Fragments arranged by size:
4629 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3989 4212 6128 7512 7780 1632
Size: 2357 223 1916 1384 268 1917
Fragments arranged by size:
2357 1917 1916 1384 268 223
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7868
AflIII A'CryG_T
Cuts at: 1203 2290 3422 4961 5064 6964 7941 1203
Size: 1087 1132 1539 103 1900 977 1327
Fragments arranged by size:
1900 1539 1327 1132 1087 977 103
AgeI A'CCGG_T
Cuts at: 6896 6896
Size: 8065
AhdI GACnn_n'nnGTC
Cuts at: 6794 6794
Size: 8065
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8065
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4164 1184
Size: 2980 5085
AlwNI CAG_nnn'CTG
Cuts at: 4300 4942 5808 7380 4300
Size: 642 866 1572 4985
Fragments arranged by size:
4985 1572 866 642
ApaI G_GGCC'C
Cuts at: 3585 4503 3585
Size: 918 7147
ApaLI G'TGCA_C
Cuts at: 3139 6122 7278 3139
Size: 2983 1156 3926
Fragments arranged by size:
3926 2983 1156
ApoI r'AATT_y
Cuts at: 1493 2471 2605 3567 4401 4409 4706 5374 5600
Size: 978 134 962 834 8 297 668 226
Cuts at: 5600 5752 7861 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 978 962 834 668 297 226
152 134 8
AseI AT'TA_AT
Cuts at: 160 1032 2439 2523 6619 160
Size: 872 1407 84 4096 1606
Fragments arranged by size:
4096 1606 1407 872 84
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8065
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3531 5390 1870
Size: 73 1048 540 1859 4545
Fragments arranged by size:
4545 1859 1048 540 73
BamHI G'GATC_C
Cuts at: 5395 5762 5395
Size: 367 7698
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3382 3616 3733 5203 5386
Size: 325 217 627 1595 234 117 1470 183
Cuts at: 5386 6841 618
Size: 1455 1842
Fragments arranged by size:
1842 1595 1470 1455 627 325 234 217
183 117
BanII G_rGCy'C
Cuts at: 1391 2992 3396 3585 4503 5384 1391
Size: 1601 404 189 918 881 4072
Fragments arranged by size:
4072 1601 918 881 404 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4394 928
Size: 971 2495 4599
Fragments arranged by size:
4599 2495 971
BbvCI CC'TCA_GC
Cuts at: 3897 4935 3897
Size: 1038 7027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6256 6290 1903
Size: 34 4319 34 3678
Fragments arranged by size:
4319 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5957 7167 5957
Size: 1210 6855
BclI T'GATC_A
Cuts at: 1548 2265 2854 1548
Size: 717 589 6759
Fragments arranged by size:
6759 717 589
BfrBI ATG'CAT
Cuts at: 2343 2343
Size: 8065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2157 2996 6676
Size: 107 122 71 631 605 485 839 3680
Cuts at: 6676 136
Size: 1525
Fragments arranged by size:
3680 1525 839 631 605 485 122 107
71
BglII A'GATC_T
Cuts at: 8060 8060
Size: 8065
BlpI GC'TnA_GC
Cuts at: 5482 5482
Size: 8065
Bme1580I G_kGCm'C
Cuts at: 1790 3143 3585 4503 6126 7282 1790
Size: 1353 442 918 1623 1156 2573
Fragments arranged by size:
2573 1623 1353 1156 918 442
BmgBI CAC'GTC
Cuts at: 1318 3329 1318
Size: 2011 6054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5181 5796 6754
Size: 416 53 83 399 24 3764 615 958
Cuts at: 6754 442
Size: 1753
Fragments arranged by size:
3764 1753 958 615 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2128 2268 2656 2667 3213 3476 3723
Size: 785 104 140 388 11 546 263 247
Cuts at: 3723 4445 6725 1239
Size: 722 2280 2579
Fragments arranged by size:
2579 2280 785 722 546 388 263 247
140 104 11
Bpu10I CC'TnA_GC
Cuts at: 3270 3897 4935 3270
Size: 627 1038 6400
Fragments arranged by size:
6400 1038 627
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 3165 3291 3620 6190 7055 7353 2486
Size: 679 126 329 2570 865 298 3198
Fragments arranged by size:
3198 2570 865 679 329 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6728 882
Size: 617 5229 2219
Fragments arranged by size:
5229 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2293 5856 493
Size: 711 1089 3563 2702
Fragments arranged by size:
3563 2702 1089 711
BsaBI GATnn'nnATC
Cuts at: 4049 8059 4049
Size: 4010 4055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5872 6254 6889 275
Size: 53 83 186 936 4339 382 635 1451
Fragments arranged by size:
4339 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2425 6498 6896 7170 7317 7728 2425
Size: 4073 398 274 147 411 2762
Fragments arranged by size:
4073 2762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2610 2640 3474 3504 3960
Size: 30 165 30 1207 30 834 30 456
Cuts at: 3960 3990 1178
Size: 30 5253
Fragments arranged by size:
5253 1207 834 456 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 3452 3743 4670 5231 1186
Size: 1084 1182 291 927 561 4020
Fragments arranged by size:
4020 1182 1084 927 561 291
BseYI C'CCAG_C
Cuts at: 7268 7764 7268
Size: 496 7569
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4996 5515 6276 7304 1540
Size: 275 186 2995 519 761 1028 2301
Fragments arranged by size:
2995 2301 1028 761 519 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2642 2745 3143 3492 3682 3975 6126 6211
Size: 629 103 398 349 190 293 2151 85
Cuts at: 6211 7282 2013
Size: 1071 2796
Fragments arranged by size:
2796 2151 1071 629 398 349 293 190
103 85
BsmI GAATG_Cn'
Cuts at: 5576 5669 5576
Size: 93 7972
BsmBI CGTCTCn'nnnn_
Cuts at: 3319 3319
Size: 8065
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2642 2745 2992 3143 3396 3492
Size: 399 223 629 103 247 151 253 96
Cuts at: 3492 3585 3682 3975 4503 5384 6126 6211 7282
Size: 93 97 293 528 881 742 85 1071
Cuts at: 7282 1391
Size: 2174
Fragments arranged by size:
2174 1071 881 742 629 528 399 293
253 247 223 151 103 97 96 93
85
BspEI T'CCGG_A
Cuts at: 7728 7728
Size: 8065
BspHI T'CATG_A
Cuts at: 2844 5954 7684 2844
Size: 3110 1730 3225
Fragments arranged by size:
3225 3110 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5420 844
Size: 4576 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5952 1394
Size: 429 4129 3507
Fragments arranged by size:
4129 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 6560 6734 65
Size: 1183 889 4423 174 1396
Fragments arranged by size:
4423 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6709 6896 1700
Size: 110 4899 187 2869
Fragments arranged by size:
4899 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2884 4956 96
Size: 2788 2072 3205
Fragments arranged by size:
3205 2788 2072
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8065
BssSI C'ACGA_G
Cuts at: 6125 7137 6125
Size: 1012 7053
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3266 3677 794
Size: 2472 411 5182
Fragments arranged by size:
5182 2472 411
BstBI TT'CG_AA
Cuts at: 3840 6869 3840
Size: 3029 5036
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8065
BstYI r'GATC_y
Cuts at: 1972 2196 5395 5762 6147 6164 7616 7702 7714
Size: 224 3199 367 385 17 1452 86 12
Cuts at: 7714 8060 1972
Size: 346 1977
Fragments arranged by size:
3199 1977 1452 385 367 346 224 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2092 513
Size: 552 939 88 6486
Fragments arranged by size:
6486 939 552 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4024 487
Size: 683 56 2798 4528
Fragments arranged by size:
4528 2798 683 56
BtsI GCAGTG_nn'
Cuts at: 2049 3222 4919 5581 6375 6395 2049
Size: 1173 1697 662 794 20 3719
Fragments arranged by size:
3719 1697 1173 794 662 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8065
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3835 3870 529
Size: 35 3271 35 4724
Fragments arranged by size:
4724 3271 35 35
DraI TTT'AAA
Cuts at: 5369 5369
Size: 8065
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7072 809
Size: 507 5756 1802
Fragments arranged by size:
5756 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4974 5145 5512
Size: 54 1425 50 275 18 3144 171 367
Cuts at: 5512 6401 8030 8
Size: 889 1629 43
Fragments arranged by size:
3144 1629 1425 889 367 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5512 1537
Size: 275 3700 4090
Fragments arranged by size:
4090 3700 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3550 4817 5123 5995 1353
Size: 2197 1267 306 872 3423
Fragments arranged by size:
3423 2197 1267 872 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6652 7026 7172 387
Size: 1064 138 5063 374 146 1280
Fragments arranged by size:
5063 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2128 2268 2656 2667 3213 3476
Size: 393 392 104 140 388 11 546 263
Cuts at: 3476 3723 3989 4212 4445 6128 6725 7512 7780
Size: 247 266 223 233 1683 597 787 268
Cuts at: 7780 1239
Size: 1524
Fragments arranged by size:
1683 1524 787 597 546 393 392 388
268 266 263 247 233 223 140 104
11
EcoNI CCTnn'n_nnAGG
Cuts at: 4875 4875
Size: 8065
EcoO109I rG'GnC_Cy
Cuts at: 3581 4696 3581
Size: 1115 6950
EcoRI G'AATT_C
Cuts at: 4409 5374 5752 4409
Size: 965 378 6722
Fragments arranged by size:
6722 965 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2426 4019 2426
Size: 1593 6472
FspI TGC'GCA
Cuts at: 6571 6571
Size: 8065
HaeII r_GCGC'y
Cuts at: 1678 4942 7212 1678
Size: 3264 2270 2531
Fragments arranged by size:
3264 2531 2270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2266 2288 2298 2320 2573
Size: 32 135 32 694 22 10 22 253
Cuts at: 2573 2605 4074 4106 4790 4822 5011 5043 6706
Size: 32 1469 32 684 32 189 32 1663
Cuts at: 6706 6738 6780 6812 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1663 1469 694 684 253 189 135
42 32 32 32 32 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 4111 4207 4341 4465 5409
Size: 988 281 151 2022 96 134 124 944
Cuts at: 5409 5655 669
Size: 246 3079
Fragments arranged by size:
3079 2022 988 944 281 246 151 134
124 96
HindIII A'AGCT_T
Cuts at: 748 2403 3359 4566 5101 748
Size: 1655 956 1207 535 3712
Fragments arranged by size:
3712 1655 1207 956 535
HpaI GTT'AAC
Cuts at: 5655 5655
Size: 8065
KpnI G_GTAC'C
Cuts at: 3620 3737 5390 3620
Size: 117 1653 6295
Fragments arranged by size:
6295 1653 117
MfeI C'AATT_G
Cuts at: 5664 5664
Size: 8065
MluI A'CGCG_T
Cuts at: 7941 7941
Size: 8065
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4648 7179 7363 1305
Size: 3343 2531 184 2007
Fragments arranged by size:
3343 2531 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4976 5147 10
Size: 54 1425 343 3144 171 2928
Fragments arranged by size:
3144 2928 1425 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3885 4053 6023 6382 6541 518
Size: 648 1182 1537 168 1970 359 159 2042
Fragments arranged by size:
2042 1970 1537 1182 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2321 3116 4559 5481 6158 7306 7551 1606
Size: 715 795 1443 922 677 1148 245 2120
Fragments arranged by size:
2120 1443 1148 922 795 715 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2092 513
Size: 552 939 88 6486
Fragments arranged by size:
6486 939 552 88
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8065
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8065
NotI GC'GGCC_GC
Cuts at: 5512 5512
Size: 8065
NsiI A_TGCA'T
Cuts at: 2345 2345
Size: 8065
NspI r_CATG'y
Cuts at: 1132 1229 3353 3426 4911 4965 5068 5333 5423
Size: 97 2124 73 1485 54 103 265 90
Cuts at: 5423 6968 1132
Size: 1545 2229
Fragments arranged by size:
2229 2124 1545 1485 265 103 97 90
73 54
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8065
PciI A'CATG_T
Cuts at: 3422 4961 5064 6964 3422
Size: 1539 103 1900 4523
Fragments arranged by size:
4523 1900 1539 103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3644 4300 1108
Size: 2536 656 4873
Fragments arranged by size:
4873 2536 656
PfoI T'CCnGG_A
Cuts at: 2284 2895 3492 3739 2284
Size: 611 597 247 6610
Fragments arranged by size:
6610 611 597 247
PmeI GTTT'AAAC
Cuts at: 5369 5369
Size: 8065
PmlI CAC'GTG
Cuts at: 2293 2293
Size: 8065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6134 6166 7647 7679 6134
Size: 32 1481 32 6520
Fragments arranged by size:
6520 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4696 4696
Size: 8065
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8065
PsiI TTA'TAA
Cuts at: 5635 5635
Size: 8065
PspOMI G'GGCC_C
Cuts at: 3581 4499 3581
Size: 918 7147
PstI C_TGCA'G
Cuts at: 830 5417 830
Size: 4587 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8065
PvuII CAG'CTG
Cuts at: 3116 4559 3116
Size: 1443 6622
SalI G'TCGA_C
Cuts at: 1936 5407 1936
Size: 3471 4594
SbfI CC_TGCA'GG
Cuts at: 5417 5417
Size: 8065
ScaI AGT'ACT
Cuts at: 6313 8025 6313
Size: 1712 6353
SfcI C'TryA_G
Cuts at: 826 1045 2758 5413 6548 7229 7420 826
Size: 219 1713 2655 1135 681 191 1471
Fragments arranged by size:
2655 1713 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8065
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8065
SmaI CCC'GGG
Cuts at: 1872 2993 5392 1872
Size: 1121 2399 4545
Fragments arranged by size:
4545 2399 1121
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 3180 3306 3599 6169 7070
Size: 197 926 558 679 126 293 2570 901
Cuts at: 7070 7332 820
Size: 262 1553
Fragments arranged by size:
2570 1553 926 901 679 558 293 262
197 126
SnaBI TAC'GTA
Cuts at: 493 5856 493
Size: 5363 2702
SpeI A'CTAG_T
Cuts at: 152 4510 5158 152
Size: 4358 648 3059
Fragments arranged by size:
4358 3059 648
SphI G_CATG'C
Cuts at: 1132 3353 5333 5423 1132
Size: 2221 1980 90 3774
Fragments arranged by size:
3774 2221 1980 90
SspI AAT'ATT
Cuts at: 5 52 2068 2509 5989 5
Size: 47 2016 441 3480 2081
Fragments arranged by size:
3480 2081 2016 441 47
StuI AGG'CCT
Cuts at: 1517 2489 3275 3298 3765 4814 1517
Size: 972 786 23 467 1049 4768
Fragments arranged by size:
4768 1049 972 786 467 23
StyI C'CwwG_G
Cuts at: 513 1065 2004 2092 3293 3387 5504 513
Size: 552 939 88 1201 94 2117 3074
Fragments arranged by size:
3074 2117 1201 939 552 94 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2712 3413 4307 4658 4982 5191 6092
Size: 1255 1132 701 894 351 324 209 901
Cuts at: 6092 6109 6262 6447 6892 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1132 901 894 701 445 351
324 209 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2884 4956 5271
Size: 274 80 33 51 1443 907 2072 315
Cuts at: 5271 6311 8023 96
Size: 1040 1712 138
Fragments arranged by size:
2072 1712 1443 1040 907 315 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2056 2088 2685 2717 8025 8057 2056
Size: 32 597 32 5308 32 2064
Fragments arranged by size:
5308 2064 597 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4115 1316
Size: 2799 5266
XbaI T'CTAG_A
Cuts at: 3724 5401 3724
Size: 1677 6388
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8065
XmaI C'CCGG_G
Cuts at: 1870 2991 5390 1870
Size: 1121 2399 4545
Fragments arranged by size:
4545 2399 1121
XmnI GAAnn'nnTTC
Cuts at: 4530 6194 4530
Size: 1664 6401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5873 276
Size: 53 83 186 936 4339 2468
Fragments arranged by size:
4339 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI