Restriction map for
pFN21AE0393
(Circular) (Six-base) MAPSORT of: pFN21AE0393.seq Check: 8311 from: 1 to: 7570
pFN21AE0393 7570 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2651 4268 2651
Size: 1617 5953
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4731 5380 278
Size: 53 83 186 936 3195 649 2468
Fragments arranged by size:
3195 2468 936 649 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2067 2145 4014 4913 1937
Size: 130 78 1869 899 4594
Fragments arranged by size:
4594 1869 899 130 78
Acc65I G'GTAC_C
Cuts at: 4891 4891
Size: 7570
AclI AA'CG_TT
Cuts at: 1563 5697 6070 1563
Size: 4134 373 3063
Fragments arranged by size:
4134 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2621 2627 2750 2877 3083 3208 4757 5633
Size: 989 6 123 127 206 125 1549 876
Cuts at: 5633 7017 7285 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1549 1384 989 876 268 206 127
125 123 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7373
AflIII A'CryG_T
Cuts at: 1203 2664 4413 4521 6469 7446 1203
Size: 1461 1749 108 1948 977 1327
Fragments arranged by size:
1948 1749 1461 1327 977 108
AgeI A'CCGG_T
Cuts at: 6401 6401
Size: 7570
AhdI GACnn_n'nnGTC
Cuts at: 2063 6299 2063
Size: 4236 3334
AleI CACnn'nnGTG
Cuts at: 1166 3225 4828 1166
Size: 2059 1603 3908
Fragments arranged by size:
3908 2059 1603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7570
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2503 2535 2503
Size: 32 7538
AlwNI CAG_nnn'CTG
Cuts at: 2389 2791 3061 3658 3748 4114 4303 5313 6885
Size: 402 270 597 90 366 189 1010 1572
Cuts at: 6885 2389
Size: 3074
Fragments arranged by size:
3074 1572 1010 597 402 366 270 189
90
ApaI G_GGCC'C
Cuts at: 4665 4839 4665
Size: 174 7396
ApaLI G'TGCA_C
Cuts at: 2411 5627 6783 2411
Size: 3216 1156 3198
Fragments arranged by size:
3216 3198 1156
ApoI r'AATT_y
Cuts at: 1493 4879 5105 5257 7366 1493
Size: 3386 226 152 2109 1697
Fragments arranged by size:
3386 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6124 160
Size: 872 5092 1606
Fragments arranged by size:
5092 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
AvaI C'yCGr_G
Cuts at: 1870 1943 2904 3463 4437 4696 4895 1870
Size: 73 961 559 974 259 199 4545
Fragments arranged by size:
4545 974 961 559 259 199 73
BamHI G'GATC_C
Cuts at: 4768 4900 5267 4768
Size: 132 367 7071
Fragments arranged by size:
7071 367 132
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2350 2716 2876 3236
Size: 325 217 627 409 154 366 160 360
Cuts at: 3236 3268 3784 4394 4891 6346 618
Size: 32 516 610 497 1455 1842
Fragments arranged by size:
1842 1455 627 610 516 497 409 366
360 325 217 160 154 32
BanII G_rGCy'C
Cuts at: 1391 2571 3210 3633 3872 4005 4230 4582 4665
Size: 1180 639 423 239 133 225 352 83
Cuts at: 4665 4807 4839 4889 1391
Size: 142 32 50 4072
Fragments arranged by size:
4072 1180 639 423 352 239 225 142
133 83 50 32
BbeI G_GCGC'C
Cuts at: 3788 3788
Size: 7570
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2462 2969 928
Size: 971 563 507 5529
Fragments arranged by size:
5529 971 563 507
BbvCI CC'TCA_GC
Cuts at: 2882 3284 2882
Size: 402 7168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2279 2313 5761 5795 1903
Size: 34 342 34 3448 34 3678
Fragments arranged by size:
3678 3448 342 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5462 6672 5462
Size: 1210 6360
BclI T'GATC_A
Cuts at: 1548 3024 1548
Size: 1476 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3466 3782 6181
Size: 107 122 71 631 605 1794 316 2399
Cuts at: 6181 136
Size: 1525
Fragments arranged by size:
2399 1794 1525 631 605 316 122 107
71
BglII A'GATC_T
Cuts at: 4281 7565 4281
Size: 3284 4286
BlpI GC'TnA_GC
Cuts at: 4987 4987
Size: 7570
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2415 3093 3271 3279 3339 4146 4647
Size: 409 216 678 178 8 60 807 501
Cuts at: 4647 4665 4839 5631 6787 1790
Size: 18 174 792 1156 2573
Fragments arranged by size:
2573 1156 807 792 678 501 409 216
178 174 60 18 8
BmgBI CAC'GTC
Cuts at: 1318 2380 3022 3646 1318
Size: 1062 642 624 5242
Fragments arranged by size:
5242 1062 642 624
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4650 5301
Size: 416 53 83 399 24 2210 1023 651
Cuts at: 5301 6259 442
Size: 958 1753
Fragments arranged by size:
2210 1753 1023 958 651 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 3497 3527 4136 6230 1239
Size: 819 1439 30 609 2094 2579
Fragments arranged by size:
2579 2094 1439 819 609 30
Bpu10I CC'TnA_GC
Cuts at: 2882 3177 3284 2882
Size: 295 107 7168
Fragments arranged by size:
7168 295 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5695 6560 6858 5695
Size: 865 298 6407
Fragments arranged by size:
6407 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2582 3898 4605 6233 882
Size: 617 1083 1316 707 1628 2219
Fragments arranged by size:
2219 1628 1316 1083 707 617
BsaAI yAC'GTr
Cuts at: 493 1204 3079 3979 5361 493
Size: 711 1875 900 1382 2702
Fragments arranged by size:
2702 1875 1382 900 711
BsaBI GATnn'nnATC
Cuts at: 7564 7564
Size: 7570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3785 3813 4728 5377
Size: 53 83 186 936 2252 28 915 649
Cuts at: 5377 5759 6394 275
Size: 382 635 1451
Fragments arranged by size:
2252 1451 936 915 649 635 382 186
83 53 28
BsaWI w'CCGG_w
Cuts at: 3487 6003 6401 6675 6822 7233 3487
Size: 2516 398 274 147 411 3824
Fragments arranged by size:
3824 2516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4187 4217 4490 4520 4526
Size: 30 165 30 2784 30 273 30 6
Cuts at: 4526 4556 1178
Size: 30 4192
Fragments arranged by size:
4192 2784 273 165 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3295 3494 1186
Size: 2109 199 5262
Fragments arranged by size:
5262 2109 199
BseYI C'CCAG_C
Cuts at: 2817 3049 3658 3705 4444 6773 7269 2817
Size: 232 609 47 739 2329 496 3118
Fragments arranged by size:
3118 2329 739 609 496 232 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2804 2911 2918 3203 3435 3617
Size: 573 666 402 107 7 285 232 182
Cuts at: 3617 3629 3868 1163
Size: 12 239 4865
Fragments arranged by size:
4865 666 573 402 285 239 232 182
107 12 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2317 3416 5020 5781 6809 1540
Size: 275 186 316 1099 1604 761 1028 2301
Fragments arranged by size:
2301 1604 1099 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 2863 3210 3426 3675 4026 5631 5716 6787
Size: 448 347 216 249 351 1605 85 1071
Cuts at: 6787 2415
Size: 3198
Fragments arranged by size:
3198 1605 1071 448 351 347 249 216
85
BsmI GAATG_Cn'
Cuts at: 4339 5081 5174 4339
Size: 742 93 6735
Fragments arranged by size:
6735 742 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2270 3859 2270
Size: 1589 5981
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2415 2571 2863 3093 3210 3271
Size: 399 409 216 156 292 230 117 61
Cuts at: 3271 3279 3339 3426 3633 3675 3872 4005 4026
Size: 8 60 87 207 42 197 133 21
Cuts at: 4026 4146 4230 4582 4647 4665 4807 4839 4889
Size: 120 84 352 65 18 142 32 50
Cuts at: 4889 5631 5716 6787 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 352 292 230
216 207 197 156 142 133 120 117
87 85 84 65 61 60 50 42
32 21 18 8
BspEI T'CCGG_A
Cuts at: 3487 7233 3487
Size: 3746 3824
BspHI T'CATG_A
Cuts at: 5459 7189 5459
Size: 1730 5840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2024 2222 2622 2651 2982 3225 4268 4925
Size: 1180 198 400 29 331 243 1043 657
Cuts at: 4925 844
Size: 3489
Fragments arranged by size:
3489 1180 1043 657 400 331 243 198
29
BsrBI CCG'CTC
Cuts at: 1394 1823 3531 5457 1394
Size: 429 1708 1926 3507
Fragments arranged by size:
3507 1926 1708 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3175 3395 4627 6065 6239 65
Size: 1183 1927 220 1232 1438 174 1396
Fragments arranged by size:
1927 1438 1396 1232 1183 220 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2353 3133 3663 6214 6401 1700
Size: 110 543 780 530 2551 187 2869
Fragments arranged by size:
2869 2551 780 543 530 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7570
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7570
BssSI C'ACGA_G
Cuts at: 5630 6642 5630
Size: 1012 6558
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2092 2791 3748 794
Size: 1298 699 957 4616
Fragments arranged by size:
4616 1298 957 699
BstBI TT'CG_AA
Cuts at: 6374 6374
Size: 7570
BstEII G'GTnAC_C
Cuts at: 1184 2011 3103 3160 3355 1184
Size: 827 1092 57 195 5399
Fragments arranged by size:
5399 1092 827 195 57
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2549 2840 2944 3426 3865 4780 4830 2374
Size: 175 291 104 482 439 915 50 5114
Fragments arranged by size:
5114 915 482 439 291 175 104 50
BstYI r'GATC_y
Cuts at: 1972 4281 4768 4900 5267 5652 5669 7121 7207
Size: 2309 487 132 367 385 17 1452 86
Cuts at: 7207 7219 7565 1972
Size: 12 346 1977
Fragments arranged by size:
2309 1977 1452 487 385 367 346 132
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4401 4401
Size: 7570
BtgI C'CryG_G
Cuts at: 513 1065 2004 2712 2833 3351 3410 4839 513
Size: 552 939 708 121 518 59 1429 3244
Fragments arranged by size:
3244 1429 939 708 552 518 121 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2442 3697 487
Size: 683 56 1216 1255 4360
Fragments arranged by size:
4360 1255 1216 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 2186 2792 2831 2846 3093 3452 3992 4804
Size: 139 606 39 15 247 359 540 812
Cuts at: 4804 5086 5880 5900 2047
Size: 282 794 20 3717
Fragments arranged by size:
3717 812 794 606 540 359 282 247
139 39 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7535
DraI TTT'AAA
Cuts at: 4874 4874
Size: 7570
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3287 6577 809
Size: 507 1971 3290 1802
Fragments arranged by size:
3290 1971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2314 2355 3413
Size: 54 1425 50 275 18 484 41 1058
Cuts at: 3413 4842 5017 5906 7535 8
Size: 1429 175 889 1629 43
Fragments arranged by size:
1629 1429 1425 1058 889 484 275 175
54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2314 3413 5017 1537
Size: 275 502 1099 1604 4090
Fragments arranged by size:
4090 1604 1099 502 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3890 5500 1353
Size: 2537 1610 3423
Fragments arranged by size:
3423 2537 1610
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3886 4034 4151 6157 6531 6677
Size: 1064 138 2297 148 117 2006 374 146
Cuts at: 6677 387
Size: 1280
Fragments arranged by size:
2297 2006 1280 1064 374 148 146 138
117
EcoICRI GAG'CTC
Cuts at: 3208 3208
Size: 7570
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 2621 2627 2750 2877 3083 3208
Size: 393 426 563 6 123 127 206 125
Cuts at: 3208 3497 3527 4136 4757 5633 6230 7017 7285
Size: 289 30 609 621 876 597 787 268
Cuts at: 7285 1239
Size: 1524
Fragments arranged by size:
1524 876 787 621 609 597 563 426
393 289 268 206 127 125 123 30
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3887 3887
Size: 7570
EcoO109I rG'GnC_Cy
Cuts at: 2596 3058 3534 4300 4662 4835 2596
Size: 462 476 766 362 173 5331
Fragments arranged by size:
5331 766 476 462 362 173
EcoRI G'AATT_C
Cuts at: 4879 5257 4879
Size: 378 7192
FseI GG_CCGG'CC
Cuts at: 3667 3667
Size: 7570
FspI TGC'GCA
Cuts at: 2285 6076 2285
Size: 3791 3779
HaeII r_GCGC'y
Cuts at: 1678 2036 2241 3659 3788 4491 6717 1678
Size: 358 205 1418 129 703 2226 2531
Fragments arranged by size:
2531 2226 1418 703 358 205 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 2516 2548 3854
Size: 32 135 32 477 32 435 32 1306
Cuts at: 3854 3886 4026 4058 4187 4219 6211 6243 6285
Size: 32 140 32 129 32 1992 32 42
Cuts at: 6285 6317 1373
Size: 32 2626
Fragments arranged by size:
2626 1992 1306 477 435 140 135 129
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2482 2557 2971 3298 4015 4914
Size: 988 281 544 75 414 327 717 899
Cuts at: 4914 5160 669
Size: 246 3079
Fragments arranged by size:
3079 988 899 717 544 414 327 281
246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7570
HpaI GTT'AAC
Cuts at: 5160 5160
Size: 7570
KasI G'GCGC_C
Cuts at: 3784 3784
Size: 7570
KpnI G_GTAC'C
Cuts at: 4895 4895
Size: 7570
MfeI C'AATT_G
Cuts at: 5169 5169
Size: 7570
MluI A'CGCG_T
Cuts at: 7446 7446
Size: 7570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 3882 6684 6868 1305
Size: 1206 1371 2802 184 2007
Fragments arranged by size:
2802 2007 1371 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4844 10
Size: 54 1425 343 3012 2736
Fragments arranged by size:
3012 2736 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2171 2372 3081 3225 3332 4152
Size: 648 843 162 201 709 144 107 820
Cuts at: 4152 4828 5528 5887 6046 518
Size: 676 700 359 159 2042
Fragments arranged by size:
2042 843 820 709 700 676 648 359
201 162 159 144 107
MspA1I CmG'CkG
Cuts at: 1606 2821 3049 3412 3543 3601 3745 3834 4111
Size: 1215 228 363 131 58 144 89 277
Cuts at: 4111 4828 4986 5663 6811 7056 1606
Size: 717 158 677 1148 245 2120
Fragments arranged by size:
2120 1215 1148 717 677 363 277 245
228 158 144 131 89 58
NaeI GCC'GGC
Cuts at: 2355 3135 3665 2355
Size: 780 530 6260
Fragments arranged by size:
6260 780 530
NarI GG'CG_CC
Cuts at: 3785 3785
Size: 7570
NcoI C'CATG_G
Cuts at: 513 1065 2004 2833 4839 513
Size: 552 939 829 2006 3244
Fragments arranged by size:
3244 2006 939 829 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7570
NgoMIV G'CCGG_C
Cuts at: 2353 3133 3663 2353
Size: 780 530 6260
Fragments arranged by size:
6260 780 530
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7570
NotI GC'GGCC_GC
Cuts at: 2314 3413 5017 2314
Size: 1099 1604 4867
Fragments arranged by size:
4867 1604 1099
NruI TCG'CGA
Cuts at: 3825 3825
Size: 7570
NspI r_CATG'y
Cuts at: 1132 1229 2099 2112 3145 4417 4525 4928 6473
Size: 97 870 13 1033 1272 108 403 1545
Cuts at: 6473 1132
Size: 2229
Fragments arranged by size:
2229 1545 1272 1033 870 403 108 97
13
PasI CC'CwG_GG
Cuts at: 1348 2765 2854 2869 3263 1348
Size: 1417 89 15 394 5655
Fragments arranged by size:
5655 1417 394 89 15
PciI A'CATG_T
Cuts at: 4413 4521 6469 4413
Size: 108 1948 5514
Fragments arranged by size:
5514 1948 108
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3658 1108
Size: 2550 5020
PfoI T'CCnGG_A
Cuts at: 2154 2253 4565 2154
Size: 99 2312 5159
Fragments arranged by size:
5159 2312 99
PmeI GTTT'AAAC
Cuts at: 4874 4874
Size: 7570
PmlI CAC'GTG
Cuts at: 3079 3079
Size: 7570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 7152 7184 5639
Size: 32 1481 32 6025
Fragments arranged by size:
6025 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 3058 3534 4300 2596
Size: 462 476 766 5866
Fragments arranged by size:
5866 766 476 462
PshAI GACnn'nnGTC
Cuts at: 1613 2594 1613
Size: 981 6589
PsiI TTA'TAA
Cuts at: 5140 5140
Size: 7570
PspOMI G'GGCC_C
Cuts at: 4661 4835 4661
Size: 174 7396
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 7538
PstI C_TGCA'G
Cuts at: 830 2181 2826 3987 4922 830
Size: 1351 645 1161 935 3478
Fragments arranged by size:
3478 1351 1161 935 645
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7570
PvuII CAG'CTG
Cuts at: 2821 3049 3601 3745 4111 4828 2821
Size: 228 552 144 366 717 5563
Fragments arranged by size:
5563 717 552 366 228 144
RsrII CG'GwC_CG
Cuts at: 4087 4087
Size: 7570
SacI G_AGCT'C
Cuts at: 3210 3210
Size: 7570
SacII CC_GC'GG
Cuts at: 3413 3413
Size: 7570
SalI G'TCGA_C
Cuts at: 1936 4013 4912 1936
Size: 2077 899 4594
Fragments arranged by size:
4594 2077 899
SbfI CC_TGCA'GG
Cuts at: 4922 4922
Size: 7570
ScaI AGT'ACT
Cuts at: 5818 7530 5818
Size: 1712 5858
SfcI C'TryA_G
Cuts at: 826 1045 2177 2822 3907 3983 4030 4918 6053
Size: 219 1132 645 1085 76 47 888 1135
Cuts at: 6053 6734 6925 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1132 1085 888 681 645 219
191 76 47
SfoI GGC'GCC
Cuts at: 3786 3786
Size: 7570
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7570
SmaI CCC'GGG
Cuts at: 1872 2906 4698 4897 1872
Size: 1034 1792 199 4545
Fragments arranged by size:
4545 1792 1034 199
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5674 6575 6837 820
Size: 197 926 3731 901 262 1553
Fragments arranged by size:
3731 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5361 493
Size: 4868 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7570
SphI G_CATG'C
Cuts at: 1132 2099 4928 1132
Size: 967 2829 3774
Fragments arranged by size:
3774 2829 967
SrfI GCCC'GGGC
Cuts at: 4698 4698
Size: 7570
SspI AAT'ATT
Cuts at: 5 52 5494 5
Size: 47 5442 2081
Fragments arranged by size:
5442 2081 47
StuI AGG'CCT
Cuts at: 1517 4435 1517
Size: 2918 4652
StyI C'CwwG_G
Cuts at: 513 1065 2004 2592 2833 3339 4839 5009 513
Size: 552 939 588 241 506 1500 170 3074
Fragments arranged by size:
3074 1500 939 588 552 506 241 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 4145 4358 5597 5614 5767 5952
Size: 1255 1266 1299 213 1239 17 153 185
Cuts at: 5952 6397 325
Size: 445 1498
Fragments arranged by size:
1498 1299 1266 1255 1239 445 213 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2474 4119 5816
Size: 274 80 33 51 1443 497 1645 1697
Cuts at: 5816 7528 96
Size: 1712 138
Fragments arranged by size:
1712 1697 1645 1443 497 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3944 3976 4526 4558 7530 7562 3944
Size: 32 550 32 2972 32 3952
Fragments arranged by size:
3952 2972 550 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4010 1316
Size: 2694 4876
XbaI T'CTAG_A
Cuts at: 4906 4906
Size: 7570
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2941 3154 4780 4852 2941
Size: 213 1626 72 5659
Fragments arranged by size:
5659 1626 213 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7570
XmaI C'CCGG_G
Cuts at: 1870 2904 4696 4895 1870
Size: 1034 1792 199 4545
Fragments arranged by size:
4545 1792 1034 199
XmnI GAAnn'nnTTC
Cuts at: 5699 5699
Size: 7570
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4729 5378 276
Size: 53 83 186 936 3195 649 2468
Fragments arranged by size:
3195 2468 936 649 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
DraIII EcoRV FalI FspAI NsiI PacI PspXI SanDI
SapI SexAI SfiI SwaI