Restriction map for
pFN21AE0391
(Circular) (Six-base) MAPSORT of: pFN21AE0391.seq Check: 1803 from: 1 to: 8296
pFN21AE0391 8296 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6106 278
Size: 53 83 186 936 4570 2468
Fragments arranged by size:
4570 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2612 5543 5575 5639 1937
Size: 675 2931 32 64 4594
Fragments arranged by size:
4594 2931 675 64 32
Acc65I G'GTAC_C
Cuts at: 5617 5617
Size: 8296
AclI AA'CG_TT
Cuts at: 1563 3400 5595 6423 6796 1563
Size: 1837 2195 828 373 3063
Fragments arranged by size:
3063 2195 1837 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2823 3684 4184 4637 5075 5294 6359
Size: 539 652 861 500 453 438 219 1065
Cuts at: 6359 7743 8011 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1065 861 652 539 500 453
438 268 219
AfeI AGC'GCT
Cuts at: 2862 2862
Size: 8296
AflII C'TTAA_G
Cuts at: 820 1017 2771 5315 820
Size: 197 1754 2544 3801
Fragments arranged by size:
3801 2544 1754 197
AflIII A'CryG_T
Cuts at: 1203 2343 3147 7195 8172 1203
Size: 1140 804 4048 977 1327
Fragments arranged by size:
4048 1327 1140 977 804
AgeI A'CCGG_T
Cuts at: 7127 7127
Size: 8296
AhdI GACnn_n'nnGTC
Cuts at: 7025 7025
Size: 8296
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8296
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2834 2981 1184
Size: 1650 147 6499
Fragments arranged by size:
6499 1650 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3364 3396 3364
Size: 32 8264
AlwNI CAG_nnn'CTG
Cuts at: 2659 6039 7611 2659
Size: 3380 1572 3344
Fragments arranged by size:
3380 3344 1572
ApaI G_GGCC'C
Cuts at: 2687 2687
Size: 8296
ApaLI G'TGCA_C
Cuts at: 6353 7509 6353
Size: 1156 7140
ApoI r'AATT_y
Cuts at: 1493 2095 2399 2752 3158 3585 3687 3950 4583
Size: 602 304 353 406 427 102 263 633
Cuts at: 4583 4685 4760 4798 4862 5292 5366 5513 5605
Size: 102 75 38 64 430 74 147 92
Cuts at: 5605 5831 5983 8092 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 633 602 430 427 406 353
304 263 226 152 147 102 102 92
75 74 64 38
AseI AT'TA_AT
Cuts at: 160 1032 2778 6850 160
Size: 872 1746 4072 1606
Fragments arranged by size:
4072 1746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8296
AvaI C'yCGr_G
Cuts at: 1870 1943 4404 4809 5621 1870
Size: 73 2461 405 812 4545
Fragments arranged by size:
4545 2461 812 405 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2553 2586 2553
Size: 33 8263
BamHI G'GATC_C
Cuts at: 2505 5626 5993 2505
Size: 3121 367 4808
Fragments arranged by size:
4808 3121 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4227 4437 5617 7072 618
Size: 325 217 627 2440 210 1180 1455 1842
Fragments arranged by size:
2440 1842 1455 1180 627 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2687 4816 5615 1391
Size: 1296 2129 799 4072
Fragments arranged by size:
4072 2129 1296 799
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 3050 4151 5529 928
Size: 971 495 656 1101 1378 3695
Fragments arranged by size:
3695 1378 1101 971 656 495
BbvCI CC'TCA_GC
Cuts at: 4104 4104
Size: 8296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6487 6521 1903
Size: 34 4550 34 3678
Fragments arranged by size:
4550 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6188 7398 6188
Size: 1210 7086
BclI T'GATC_A
Cuts at: 1548 5212 1548
Size: 3664 4632
BfrBI ATG'CAT
Cuts at: 5476 5476
Size: 8296
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6907 136
Size: 107 122 71 631 605 5235 1525
Fragments arranged by size:
5235 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3753 8291 3753
Size: 4538 3758
BlpI GC'TnA_GC
Cuts at: 4444 5713 4444
Size: 1269 7027
Bme1580I G_kGCm'C
Cuts at: 1790 2687 2877 6357 7513 1790
Size: 897 190 3480 1156 2573
Fragments arranged by size:
3480 2573 1156 897 190
BmgBI CAC'GTC
Cuts at: 1318 2884 3139 1318
Size: 1566 255 6475
Fragments arranged by size:
6475 1566 255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6027 6985 442
Size: 416 53 83 399 24 4610 958 1753
Fragments arranged by size:
4610 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4844 1056
Size: 3788 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4196 4262 4323 6956 1239
Size: 2957 66 61 2633 2579
Fragments arranged by size:
2957 2633 2579 66 61
Bpu10I CC'TnA_GC
Cuts at: 4104 4520 4104
Size: 416 7880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2917 3611 5192 6421 7286 7584 2917
Size: 694 1581 1229 865 298 3629
Fragments arranged by size:
3629 1581 1229 865 694 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6959 882
Size: 617 5460 2219
Fragments arranged by size:
5460 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 3512 6087 493
Size: 711 1946 362 2575 2702
Fragments arranged by size:
2702 2575 1946 711 362
BsaBI GATnn'nnATC
Cuts at: 8290 8290
Size: 8296
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6103 6485 7120 275
Size: 53 83 186 936 4570 382 635 1451
Fragments arranged by size:
4570 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6729 7127 7401 7548 7959 6729
Size: 398 274 147 411 7066
Fragments arranged by size:
7066 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3218 3248 1178
Size: 30 165 30 1815 30 6226
Fragments arranged by size:
6226 1815 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2103 3239 4686 5313 1186
Size: 917 1136 1447 627 4169
Fragments arranged by size:
4169 1447 1136 917 627
BseYI C'CCAG_C
Cuts at: 7499 7995 7499
Size: 496 7800
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2281 2794 3503 5397 1163
Size: 573 545 513 709 1894 4062
Fragments arranged by size:
4062 1894 709 573 545 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5746 6507 7535 1540
Size: 275 186 3745 761 1028 2301
Fragments arranged by size:
3745 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4163 6357 6442 7513 4163
Size: 2194 85 1071 4946
Fragments arranged by size:
4946 2194 1071 85
BsmI GAATG_Cn'
Cuts at: 3043 4821 5478 5807 5900 3043
Size: 1778 657 329 93 5439
Fragments arranged by size:
5439 1778 657 329 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2687 2877 4163 4816 5615 6357 6442
Size: 399 897 190 1286 653 799 742 85
Cuts at: 6442 7513 1391
Size: 1071 2174
Fragments arranged by size:
2174 1286 1071 897 799 742 653 399
190 85
BspEI T'CCGG_A
Cuts at: 7959 7959
Size: 8296
BspHI T'CATG_A
Cuts at: 4641 6185 7915 4641
Size: 1544 1730 5022
Fragments arranged by size:
5022 1730 1544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3730 5035 5651 844
Size: 2886 1305 616 3489
Fragments arranged by size:
3489 2886 1305 616
BsrBI CCG'CTC
Cuts at: 1394 1823 6183 1394
Size: 429 4360 3507
Fragments arranged by size:
4360 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 6791 6965 65
Size: 1183 906 4637 174 1396
Fragments arranged by size:
4637 1396 1183 906 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6940 7127 1700
Size: 110 5130 187 2869
Fragments arranged by size:
5130 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5376 96
Size: 5280 3016
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8296
BssSI C'ACGA_G
Cuts at: 6356 7368 6356
Size: 1012 7284
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4244 4466 794
Size: 3450 222 4624
Fragments arranged by size:
4624 3450 222
BstBI TT'CG_AA
Cuts at: 7100 7100
Size: 8296
BstEII G'GTnAC_C
Cuts at: 1184 5022 1184
Size: 3838 4458
BstYI r'GATC_y
Cuts at: 1972 2254 2505 3753 4384 5527 5626 5993 6378
Size: 282 251 1248 631 1143 99 367 385
Cuts at: 6378 6395 7847 7933 7945 8291 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1248 1143 631 385 367 346
282 251 99 86 17 12
BstZ17I GTA'TAC
Cuts at: 2613 5544 2613
Size: 2931 5365
BtgI C'CryG_G
Cuts at: 513 1065 2877 513
Size: 552 1812 5932
Fragments arranged by size:
5932 1812 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7557
Fragments arranged by size:
7557 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 5082 5812 6606 6626 2256
Size: 2826 730 794 20 3926
Fragments arranged by size:
3926 2826 794 730 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8296
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5058 5093 529
Size: 35 4494 35 3732
Fragments arranged by size:
4494 3732 35 35
DraI TTT'AAA
Cuts at: 4629 5600 4629
Size: 971 7325
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7303 809
Size: 507 5987 1802
Fragments arranged by size:
5987 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3859 4941 4974
Size: 54 1425 50 275 18 2029 1082 33
Cuts at: 4974 5743 6632 8261 8
Size: 769 889 1629 43
Fragments arranged by size:
2029 1629 1425 1082 889 769 275 54
50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 5743 1537
Size: 275 3931 4090
Fragments arranged by size:
4090 3931 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2842 3455 3996 4783 5037 5142 6226 1353
Size: 1489 613 541 787 254 105 1084 3423
Fragments arranged by size:
3423 1489 1084 787 613 541 254 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6883 7257 7403 387
Size: 1064 138 5294 374 146 1280
Fragments arranged by size:
5294 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2823 3684 4184 4196 4262 4323
Size: 393 539 652 861 500 12 66 61
Cuts at: 4323 4637 5075 5294 6359 6956 7743 8011 1239
Size: 314 438 219 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 861 787 652 597 539 500
438 393 314 268 219 66 61 12
EcoO109I rG'GnC_Cy
Cuts at: 2683 5008 2683
Size: 2325 5971
EcoRI G'AATT_C
Cuts at: 2399 4583 5605 5983 2399
Size: 2184 1022 378 4712
Fragments arranged by size:
4712 2184 1022 378
EcoRV GAT'ATC
Cuts at: 3681 3681
Size: 8296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3105 3105
Size: 8296
FspI TGC'GCA
Cuts at: 6802 6802
Size: 8296
HaeII r_GCGC'y
Cuts at: 1678 2653 2864 7443 1678
Size: 975 211 4579 2531
Fragments arranged by size:
4579 2531 975 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2042 2068 2074 3218
Size: 32 135 32 464 6 26 6 1144
Cuts at: 3218 3231 3250 3263 3464 3496 4138 4170 5204
Size: 13 19 13 201 32 642 32 1034
Cuts at: 5204 5236 5519 5551 6937 6969 7011 7043 1373
Size: 32 283 32 1386 32 42 32 2626
Fragments arranged by size:
2626 1386 1144 1034 642 464 283 201
135 42 32 32 32 32 32 32
32 32 26 19 13 13 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4806 5485 5640 5886 669
Size: 988 281 2868 679 155 246 3079
Fragments arranged by size:
3079 2868 988 679 281 246 155
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8296
HpaI GTT'AAC
Cuts at: 4806 5485 5886 4806
Size: 679 401 7216
Fragments arranged by size:
7216 679 401
KpnI G_GTAC'C
Cuts at: 5621 5621
Size: 8296
MfeI C'AATT_G
Cuts at: 3810 3855 4857 5895 3810
Size: 45 1002 1038 6211
Fragments arranged by size:
6211 1038 1002 45
MluI A'CGCG_T
Cuts at: 8172 8172
Size: 8296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2974 7410 7594 1305
Size: 1669 4436 184 2007
Fragments arranged by size:
4436 2007 1669 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3861 4943 4976 10
Size: 54 1425 343 2029 1082 33 3330
Fragments arranged by size:
3330 2029 1425 1082 343 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 2166 6254 6613 6772 518
Size: 648 1000 4088 359 159 2042
Fragments arranged by size:
4088 2042 1000 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4166 4204 5712 6389 7537 7782 1606
Size: 2560 38 1508 677 1148 245 2120
Fragments arranged by size:
2560 2120 1508 1148 677 245 38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7744
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8296
NheI G'CTAG_C
Cuts at: 1052 4840 1052
Size: 3788 4508
NotI GC'GGCC_GC
Cuts at: 5743 5743
Size: 8296
NsiI A_TGCA'T
Cuts at: 5478 5478
Size: 8296
NspI r_CATG'y
Cuts at: 1132 1229 2347 3709 4078 5654 7199 1132
Size: 97 1118 1362 369 1576 1545 2229
Fragments arranged by size:
2229 1576 1545 1362 1118 369 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8296
PciI A'CATG_T
Cuts at: 2343 7195 2343
Size: 4852 3444
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8296
PfoI T'CCnGG_A
Cuts at: 4278 4339 4278
Size: 61 8235
PmeI GTTT'AAAC
Cuts at: 5600 5600
Size: 8296
PmlI CAC'GTG
Cuts at: 3150 3150
Size: 8296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2136 2168 6365 6397 7878 7910 2136
Size: 32 4197 32 1481 32 2522
Fragments arranged by size:
4197 2522 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5008 5008
Size: 8296
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8296
PsiI TTA'TAA
Cuts at: 4038 5866 4038
Size: 1828 6468
PspOMI G'GGCC_C
Cuts at: 2683 2683
Size: 8296
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2217 2249 5121 5153 2217
Size: 32 2872 32 5360
Fragments arranged by size:
5360 2872 32 32
PstI C_TGCA'G
Cuts at: 830 2141 3727 3844 4213 4224 4578 5648 830
Size: 1311 1586 117 369 11 354 1070 3478
Fragments arranged by size:
3478 1586 1311 1070 369 354 117 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8296
PvuII CAG'CTG
Cuts at: 4204 4204
Size: 8296
SalI G'TCGA_C
Cuts at: 1936 5638 1936
Size: 3702 4594
SapI GCTCTTCn'nnn_
Cuts at: 5037 5037
Size: 8296
SbfI CC_TGCA'GG
Cuts at: 3727 5648 3727
Size: 1921 6375
ScaI AGT'ACT
Cuts at: 6544 8256 6544
Size: 1712 6584
SfcI C'TryA_G
Cuts at: 826 1045 2137 3217 3723 3840 4209 4220 4574
Size: 219 1092 1080 506 117 369 11 354
Cuts at: 4574 5062 5644 6779 7460 7651 826
Size: 488 582 1135 681 191 1471
Fragments arranged by size:
1471 1135 1092 1080 681 582 506 488
369 354 219 191 117 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8296
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8296
SmaI CCC'GGG
Cuts at: 1872 5623 1872
Size: 3751 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2771 2896 3626 5171 5315 6400
Size: 197 926 828 125 730 1545 144 1085
Cuts at: 6400 7301 7563 820
Size: 901 262 1553
Fragments arranged by size:
1553 1545 1085 926 901 828 730 262
197 144 125
SnaBI TAC'GTA
Cuts at: 493 6087 493
Size: 5594 2702
SpeI A'CTAG_T
Cuts at: 152 2566 152
Size: 2414 5882
SphI G_CATG'C
Cuts at: 1132 4078 5654 1132
Size: 2946 1576 3774
Fragments arranged by size:
3774 2946 1576
SspI AAT'ATT
Cuts at: 5 52 2428 3572 4412 6220 5
Size: 47 2376 1144 840 1808 2081
Fragments arranged by size:
2376 2081 1808 1144 840 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8296
StyI C'CwwG_G
Cuts at: 513 1065 5735 513
Size: 552 4670 3074
Fragments arranged by size:
4670 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2100 6323 6340 6493 6678 7123 325
Size: 1255 520 4223 17 153 185 445 1498
Fragments arranged by size:
4223 1498 1255 520 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4309 5376 6542
Size: 274 80 33 51 1443 2332 1067 1166
Cuts at: 6542 8254 96
Size: 1712 138
Fragments arranged by size:
2332 1712 1443 1166 1067 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4312 4344 4956 4988 8256 8288 4312
Size: 32 612 32 3268 32 4320
Fragments arranged by size:
4320 3268 612 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4150 1316
Size: 2834 5462
XbaI T'CTAG_A
Cuts at: 3610 5632 3610
Size: 2022 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3692 3692
Size: 8296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8296
XmaI C'CCGG_G
Cuts at: 1870 5621 1870
Size: 3751 4545
XmnI GAAnn'nnTTC
Cuts at: 5338 6425 5338
Size: 1087 7209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6104 276
Size: 53 83 186 936 4570 2468
Fragments arranged by size:
4570 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BplI BsiWI BsmBI BstXI
Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PspXI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI