Restriction map for
pFN21AE0390
(Circular) (Six-base) MAPSORT of: pFN21AE0390.seq Check: 9182 from: 1 to: 6169
pFN21AE0390 6169 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3231 3979 278
Size: 53 83 186 936 1695 748 2468
Fragments arranged by size:
2468 1695 936 748 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3369 3512 1937
Size: 1432 143 4594
Fragments arranged by size:
4594 1432 143
Acc65I G'GTAC_C
Cuts at: 3330 3490 3330
Size: 160 6009
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2855 4232 5616 5884 1632
Size: 1223 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 1223 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 2667 5068 6045 1203
Size: 1464 2401 977 1327
Fragments arranged by size:
2401 1464 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 3232 4898 3232
Size: 1666 4503
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 5014
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2238 2401 2833 3188 3912 5484 2238
Size: 163 432 355 724 1572 2923
Fragments arranged by size:
2923 1572 724 432 355 163
ApaI G_GGCC'C
Cuts at: 2491 2968 2491
Size: 477 5692
ApaLI G'TGCA_C
Cuts at: 3044 4226 5382 3044
Size: 1182 1156 3831
Fragments arranged by size:
3831 1182 1156
ApoI r'AATT_y
Cuts at: 1493 2690 3478 3704 3856 5965 1493
Size: 1197 788 226 152 2109 1697
Fragments arranged by size:
2109 1697 1197 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2096 2462 2656 2881 2954 3148 3404
Size: 73 153 366 194 225 73 194 256
Cuts at: 3404 3494 1870
Size: 90 4545
Fragments arranged by size:
4545 366 256 225 194 194 153 90
73 73
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2090 2948 3330 3490 4945
Size: 325 217 627 303 858 382 160 1455
Cuts at: 4945 618
Size: 1842
Fragments arranged by size:
1842 1455 858 627 382 325 303 217
160
BanII G_rGCy'C
Cuts at: 1391 2307 2491 2759 2968 3488 1391
Size: 916 184 268 209 520 4072
Fragments arranged by size:
4072 916 520 268 209 184
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3202 928
Size: 971 1303 3895
Fragments arranged by size:
3895 1303 971
BbvCI CC'TCA_GC
Cuts at: 2163 3009 2163
Size: 846 5323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2862 2896 4360 4394 1903
Size: 34 925 34 1464 34 3678
Fragments arranged by size:
3678 1464 925 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2466 3359 4780
Size: 107 122 71 631 605 794 893 1421
Cuts at: 4780 136
Size: 1525
Fragments arranged by size:
1525 1421 893 794 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2058 6164 2058
Size: 4106 2063
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2245 2313 2491 2951 2968 3048 4230
Size: 340 115 68 178 460 17 80 1182
Cuts at: 4230 5386 1790
Size: 1156 2573
Fragments arranged by size:
2573 1182 1156 460 340 178 115 80
68 17
BmgBI CAC'GTC
Cuts at: 1318 2668 1318
Size: 1350 4819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 3900 4858
Size: 416 53 83 399 24 700 1783 958
Cuts at: 4858 442
Size: 1753
Fragments arranged by size:
1783 1753 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2594 3047 3404 3412 4829 1239
Size: 1355 453 357 8 1417 2579
Fragments arranged by size:
2579 1417 1355 453 357 8
Bpu10I CC'TnA_GC
Cuts at: 2163 3009 2163
Size: 846 5323
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3113 4294 5159 5457 3113
Size: 1181 865 298 3825
Fragments arranged by size:
3825 1181 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2521 4832 882
Size: 617 1022 2311 2219
Fragments arranged by size:
2311 2219 1022 617
BsaAI yAC'GTr
Cuts at: 493 1204 2323 3373 3960 493
Size: 711 1119 1050 587 2702
Fragments arranged by size:
2702 1119 1050 711 587
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3228 3243 3976 4358
Size: 53 83 186 936 1695 15 733 382
Cuts at: 4358 4993 275
Size: 635 1451
Fragments arranged by size:
1695 1451 936 733 635 382 186 83
53 15
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2501 2531 2882 2912 3128
Size: 30 165 30 1098 30 351 30 216
Cuts at: 3128 3158 1178
Size: 30 4189
Fragments arranged by size:
4189 1098 351 216 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2591 3044 3409 1186
Size: 1210 195 453 365 3946
Fragments arranged by size:
3946 1210 453 365 195
BseYI C'CCAG_C
Cuts at: 2294 2447 2710 2752 5372 5868 2294
Size: 153 263 42 2620 496 2595
Fragments arranged by size:
2620 2595 496 263 153 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2852 3236 3428 1163
Size: 573 1116 384 192 3904
Fragments arranged by size:
3904 1116 573 384 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2882 3048 3075 3378 4230 4315 5386 2882
Size: 166 27 303 852 85 1071 3665
Fragments arranged by size:
3665 1071 852 303 166 85 27
BsmI GAATG_Cn'
Cuts at: 3680 3773 3680
Size: 93 6076
BsmBI CGTCTCn'nnnn_
Cuts at: 2336 3299 2336
Size: 963 5206
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2245 2307 2313 2491 2759 2882
Size: 399 340 115 62 6 178 268 123
Cuts at: 2882 2951 2968 3048 3075 3378 3488 4230 4315
Size: 69 17 80 27 303 110 742 85
Cuts at: 4315 5386 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 303 268 178
123 115 110 85 80 69 62 27
17 6
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2553 2940 3524 844
Size: 1709 387 584 3489
Fragments arranged by size:
3489 1709 584 387
BsrBI CCG'CTC
Cuts at: 1394 1823 2677 2854 4056 1394
Size: 429 854 177 1202 3507
Fragments arranged by size:
3507 1202 854 429 177
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 3333 4813 5000 1700
Size: 110 255 1268 1480 187 2869
Fragments arranged by size:
2869 1480 1268 255 187 110
BsrGI T'GTAC_A
Cuts at: 96 2898 96
Size: 2802 3367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2306 2849 3074 3254 3302 4229 5241 2306
Size: 543 225 180 48 927 1012 3234
Fragments arranged by size:
3234 1012 927 543 225 180 48
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3322 794
Size: 2528 3641
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2058 2823 3165 3499 3866 4251 4268 5720
Size: 86 765 342 334 367 385 17 1452
Cuts at: 5720 5806 5818 6164 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 765 385 367 346 342 334
86 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2018 3189 2018
Size: 1171 4998
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 2224 2520 2766 513
Size: 552 939 82 138 296 246 3916
Fragments arranged by size:
3916 939 552 296 246 138 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 2880 3685 4479 4499 2880
Size: 805 794 20 4550
Fragments arranged by size:
4550 805 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 2875 2875
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2063 2325 2678
Size: 54 1425 50 275 18 233 262 353
Cuts at: 2678 3057 3195 3271 3616 4505 6134 8
Size: 379 138 76 345 889 1629 43
Fragments arranged by size:
1629 1425 889 379 353 345 275 262
233 138 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2174 2244 4099 1353
Size: 821 70 1855 3423
Fragments arranged by size:
3423 1855 821 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2210 3377 4756 5130 5276 387
Size: 1064 138 621 1167 1379 374 146 1280
Fragments arranged by size:
1379 1280 1167 1064 621 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2594 2855 3047 3404 3412 4232 4829
Size: 393 962 261 192 357 8 820 597
Cuts at: 4829 5616 5884 1239
Size: 787 268 1524
Fragments arranged by size:
1524 962 820 787 597 393 357 268
261 192 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 2549 2816 2249
Size: 300 267 5602
Fragments arranged by size:
5602 300 267
EcoO109I rG'GnC_Cy
Cuts at: 2100 2430 2653 2839 2965 2100
Size: 330 223 186 126 5304
Fragments arranged by size:
5304 330 223 186 126
EcoRI G'AATT_C
Cuts at: 2690 3478 3856 2690
Size: 788 378 5003
Fragments arranged by size:
5003 788 378
FspI TGC'GCA
Cuts at: 2921 4675 2921
Size: 1754 4415
HaeII r_GCGC'y
Cuts at: 1678 2301 3188 5316 1678
Size: 623 887 2128 2531
Fragments arranged by size:
2531 2128 887 623
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3128 3160 3218 3250 4810
Size: 32 135 32 1556 32 58 32 1560
Cuts at: 4810 4842 4884 4916 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1560 1556 135 58 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3513 3759 669
Size: 988 281 1575 246 3079
Fragments arranged by size:
3079 1575 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3334 3494 3334
Size: 160 6009
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2363 5283 5467 1305
Size: 1058 2920 184 2007
Fragments arranged by size:
2920 2007 1058 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2327 3273 10
Size: 54 1425 343 495 946 2906
Fragments arranged by size:
2906 1425 946 495 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 4127 4486 4645 518
Size: 648 1155 1806 359 159 2042
Fragments arranged by size:
2042 1806 1155 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2226 2401 2528 3585 4262 5410 5655 1606
Size: 620 175 127 1057 677 1148 245 2120
Fragments arranged by size:
2120 1148 1057 677 620 245 175 127
NaeI GCC'GGC
Cuts at: 2067 2067
Size: 6169
NcoI C'CATG_G
Cuts at: 513 1065 2004 2086 2520 513
Size: 552 939 82 434 4162
Fragments arranged by size:
4162 939 552 434 82
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NgoMIV G'CCGG_C
Cuts at: 2065 2065
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 2644 3527 5072 1132
Size: 97 1415 883 1545 2229
Fragments arranged by size:
2229 1545 1415 883 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PmlI CAC'GTG
Cuts at: 2323 2323
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2100
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2487 2964 2487
Size: 477 5692
PstI C_TGCA'G
Cuts at: 830 2550 2634 2937 3315 3521 830
Size: 1720 84 303 378 206 3478
Fragments arranged by size:
3478 1720 378 303 206 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2401 2528 2401
Size: 127 6042
SacII CC_GC'GG
Cuts at: 2227 2227
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SanDI GG'GwC_CC
Cuts at: 2100 2100
Size: 6169
SapI GCTCTTCn'nnn_
Cuts at: 2174 2174
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 2937 3521 2937
Size: 584 5585
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2546 2630 2933 3311 3517 4652 5333
Size: 219 1501 84 303 378 206 1135 681
Cuts at: 5333 5524 826
Size: 191 1471
Fragments arranged by size:
1501 1471 1135 681 378 303 219 206
191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2098 3496 1872
Size: 226 1398 4545
Fragments arranged by size:
4545 1398 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3128 4273 5174 5436 820
Size: 197 926 1185 1145 901 262 1553
Fragments arranged by size:
1553 1185 1145 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 2644 3527 1132
Size: 1512 883 3774
Fragments arranged by size:
3774 1512 883
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 2745 2939 1517
Size: 1228 194 4747
Fragments arranged by size:
4747 1228 194
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 2328 2496 2520 2698 2817
Size: 552 939 82 242 168 24 178 119
Cuts at: 2817 3608 513
Size: 791 3074
Fragments arranged by size:
3074 939 791 552 242 178 168 119
82 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2333 2431 2702 4196 4213 4366 4551
Size: 1255 753 98 271 1494 17 153 185
Cuts at: 4551 4996 325
Size: 445 1498
Fragments arranged by size:
1498 1494 1255 753 445 271 185 153
98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 4415 6127
Size: 274 80 33 51 1443 921 1517 1712
Cuts at: 6127 96
Size: 138
Fragments arranged by size:
1712 1517 1443 921 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2466 2498 2698 2730 2732 2764 6129 6161 2466
Size: 32 200 32 2 32 3365 32 2474
Fragments arranged by size:
3365 2474 200 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2320
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2096 3494 1870
Size: 226 1398 4545
Fragments arranged by size:
4545 1398 226
XmnI GAAnn'nnTTC
Cuts at: 4298 4298
Size: 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3229 3977 276
Size: 53 83 186 936 1695 748 2468
Fragments arranged by size:
2468 1695 936 748 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PspXI PsrI
RsrII SacI SexAI SfiI SfoI SrfI SwaI