Restriction map for
pFN21AE0389
(Circular) (Six-base) MAPSORT of: pFN21AE0389.seq Check: 7620 from: 1 to: 7213
pFN21AE0389 7213 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 3327 2790
Size: 537 6676
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5023 278
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3675 4556 1937
Size: 1738 881 4594
Fragments arranged by size:
4594 1738 881
Acc65I G'GTAC_C
Cuts at: 4534 4534
Size: 7213
AclI AA'CG_TT
Cuts at: 1563 5340 5713 1563
Size: 3777 373 3063
Fragments arranged by size:
3777 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 2614 3427 3844 5276 6660 6928 1632
Size: 689 293 813 417 1432 1384 268 1917
Fragments arranged by size:
1917 1432 1384 813 689 417 293 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7016
AflIII A'CryG_T
Cuts at: 1203 3218 6112 7089 1203
Size: 2015 2894 977 1327
Fragments arranged by size:
2894 2015 1327 977
AgeI A'CCGG_T
Cuts at: 3264 6044 3264
Size: 2780 4433
AhdI GACnn_n'nnGTC
Cuts at: 2302 5942 2302
Size: 3640 3573
AleI CACnn'nnGTG
Cuts at: 1166 3223 3722 1166
Size: 2057 499 4657
Fragments arranged by size:
4657 2057 499
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7213
AlwNI CAG_nnn'CTG
Cuts at: 3403 3481 3601 3912 4956 6528 3403
Size: 78 120 311 1044 1572 4088
Fragments arranged by size:
4088 1572 1044 311 120 78
ApaI G_GGCC'C
Cuts at: 4109 4354 4109
Size: 245 6968
ApaLI G'TGCA_C
Cuts at: 5270 6426 5270
Size: 1156 6057
ApoI r'AATT_y
Cuts at: 1493 4522 4748 4900 7009 1493
Size: 3029 226 152 2109 1697
Fragments arranged by size:
3029 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2066 2066
Size: 7213
AseI AT'TA_AT
Cuts at: 160 1032 5767 160
Size: 872 4735 1606
Fragments arranged by size:
4735 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
AvaI C'yCGr_G
Cuts at: 1870 1943 2860 2879 3502 3843 4055 4538 1870
Size: 73 917 19 623 341 212 483 4545
Fragments arranged by size:
4545 917 623 483 341 212 73 19
BamHI G'GATC_C
Cuts at: 2976 4543 4910 2976
Size: 1567 367 5279
Fragments arranged by size:
5279 1567 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 3358 3388 4304 4534
Size: 325 217 627 1511 60 30 916 230
Cuts at: 4534 5989 618
Size: 1455 1842
Fragments arranged by size:
1842 1511 1455 916 627 325 230 217
60 30
BanII G_rGCy'C
Cuts at: 1391 2751 2867 4062 4109 4354 4497 4532 1391
Size: 1360 116 1195 47 245 143 35 4072
Fragments arranged by size:
4072 1360 1195 245 143 116 47 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4100 928
Size: 971 2201 4041
Fragments arranged by size:
4041 2201 971
BbvCI CC'TCA_GC
Cuts at: 3849 3849
Size: 7213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5404 5438 1903
Size: 34 3467 34 3678
Fragments arranged by size:
3678 3467 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5105 6315 5105
Size: 1210 6003
BclI T'GATC_A
Cuts at: 1548 3822 1548
Size: 2274 4939
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3507 5824 136
Size: 107 122 71 631 605 1835 2317 1525
Fragments arranged by size:
2317 1835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3855 7208 3855
Size: 3353 3860
BlpI GC'TnA_GC
Cuts at: 2698 3374 4630 2698
Size: 676 1256 5281
Fragments arranged by size:
5281 1256 676
Bme1580I G_kGCm'C
Cuts at: 1790 3303 3363 3747 4109 4307 4354 5274 6430
Size: 1513 60 384 362 198 47 920 1156
Cuts at: 6430 1790
Size: 2573
Fragments arranged by size:
2573 1513 1156 920 384 362 198 60
47
BmgBI CAC'GTC
Cuts at: 1318 2757 3221 1318
Size: 1439 464 5310
Fragments arranged by size:
5310 1439 464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 3703 4944
Size: 416 53 83 399 24 1880 406 1241
Cuts at: 4944 5902 442
Size: 958 1753
Fragments arranged by size:
1880 1753 1241 958 416 406 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2241 2915 3278 3574 4330 5873 1239
Size: 1002 674 363 296 756 1543 2579
Fragments arranged by size:
2579 1543 1002 756 674 363 296
Bpu10I CC'TnA_GC
Cuts at: 3849 3849
Size: 7213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3070 5338 6203 6501 3070
Size: 2268 865 298 3782
Fragments arranged by size:
3782 2268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2032 3744 5876 882
Size: 617 533 1712 2132 2219
Fragments arranged by size:
2219 2132 1712 617 533
BsaAI yAC'GTr
Cuts at: 493 1204 3040 5004 493
Size: 711 1836 1964 2702
Fragments arranged by size:
2702 1964 1836 711
BsaBI GATnn'nnATC
Cuts at: 7207 7207
Size: 7213
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5020 5402 6037 275
Size: 53 83 186 936 3487 382 635 1451
Fragments arranged by size:
3487 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3264 5646 6044 6318 6465 6876 3264
Size: 2382 398 274 147 411 3601
Fragments arranged by size:
3601 2382 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3179 3209 3476 3506 4020
Size: 30 165 30 1776 30 267 30 514
Cuts at: 4020 4050 4178 4208 4412 4442 1178
Size: 30 128 30 204 30 3949
Fragments arranged by size:
3949 1776 514 267 204 165 128 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2852 3866 4377 4391 1186
Size: 1666 1014 511 14 4008
Fragments arranged by size:
4008 1666 1014 511 14
BseYI C'CCAG_C
Cuts at: 2056 2140 3470 3569 6416 6912 2056
Size: 84 1330 99 2847 496 2357
Fragments arranged by size:
2847 2357 1330 496 99 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2760 3238 1163
Size: 573 1024 478 5138
Fragments arranged by size:
5138 1024 573 478
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2500 4295 4663 5424 6452 1540
Size: 275 186 499 1795 368 761 1028 2301
Fragments arranged by size:
2301 1795 1028 761 499 368 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 3882 5274 5359 6430 2751
Size: 1131 1392 85 1071 3534
Fragments arranged by size:
3534 1392 1131 1071 85
BsmI GAATG_Cn'
Cuts at: 3815 4724 4817 3815
Size: 909 93 6211
Fragments arranged by size:
6211 909 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2800 2800
Size: 7213
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2751 2867 3303 3363 3747 3882 4062
Size: 399 961 116 436 60 384 135 180
Cuts at: 4062 4109 4307 4354 4497 4532 5274 5359 6430
Size: 47 198 47 143 35 742 85 1071
Cuts at: 6430 1391
Size: 2174
Fragments arranged by size:
2174 1071 961 742 436 399 384 198
180 143 135 116 85 60 47 47
35
BspEI T'CCGG_A
Cuts at: 6876 6876
Size: 7213
BspHI T'CATG_A
Cuts at: 2751 3819 5102 6832 2751
Size: 1068 1283 1730 3132
Fragments arranged by size:
3132 1730 1283 1068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2790 3158 3327 3969 4568 844
Size: 1946 368 169 642 599 3489
Fragments arranged by size:
3489 1946 642 599 368 169
BsrBI CCG'CTC
Cuts at: 1394 1823 2646 2842 3784 3789 3945 5100 1394
Size: 429 823 196 942 5 156 1155 3507
Fragments arranged by size:
3507 1155 942 823 429 196 156 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 2548 2634 5708 5882 65
Size: 1183 1300 86 3074 174 1396
Fragments arranged by size:
3074 1396 1300 1183 174 86
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 3264 4290 5857 6044 1700
Size: 110 199 1255 1026 1567 187 2869
Fragments arranged by size:
2869 1567 1255 1026 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7213
BssHII G'CGCG_C
Cuts at: 1925 2066 3811 1925
Size: 141 1745 5327
Fragments arranged by size:
5327 1745 141
BssSI C'ACGA_G
Cuts at: 2109 3419 3452 3940 5273 6285 2109
Size: 1310 33 488 1333 1012 3037
Fragments arranged by size:
3037 1333 1310 1012 488 33
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7213
BstBI TT'CG_AA
Cuts at: 6017 6017
Size: 7213
BstEII G'GTnAC_C
Cuts at: 1184 3547 3649 1184
Size: 2363 102 4748
Fragments arranged by size:
4748 2363 102
BstYI r'GATC_y
Cuts at: 1972 2280 2976 3522 3855 4543 4910 5295 5312
Size: 308 696 546 333 688 367 385 17
Cuts at: 5312 6764 6850 6862 7208 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 696 688 546 385 367 346
333 308 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2441 2739 3680 2441
Size: 298 941 5974
Fragments arranged by size:
5974 941 298
BtgI C'CryG_G
Cuts at: 513 1065 2004 2514 2600 3086 4224 4308 4497
Size: 552 939 510 86 486 1138 84 189
Cuts at: 4497 513
Size: 3229
Fragments arranged by size:
3229 1138 939 552 510 486 189 86
84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4000 487
Size: 683 56 2774 3700
Fragments arranged by size:
3700 2774 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 4729 5523 5543 2036
Size: 2693 794 20 3706
Fragments arranged by size:
3706 2693 794 20
ClaI AT'CG_AT
Cuts at: 1554 2505 1554
Size: 951 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3975 4010 529
Size: 35 3411 35 3732
Fragments arranged by size:
3732 3411 35 35
DraI TTT'AAA
Cuts at: 4517 4517
Size: 7213
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4004 6220 809
Size: 507 2688 2216 1802
Fragments arranged by size:
2688 2216 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2497 3456
Size: 54 1425 50 275 18 177 490 959
Cuts at: 3456 4292 4368 4660 5549 7178 8
Size: 836 76 292 889 1629 43
Fragments arranged by size:
1629 1425 959 889 836 490 292 275
177 76 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2497 4292 4660 1537
Size: 275 685 1795 368 4090
Fragments arranged by size:
4090 1795 685 368 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3971 5143 1353
Size: 2618 1172 3423
Fragments arranged by size:
3423 2618 1172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5800 6174 6320 387
Size: 1064 138 4211 374 146 1280
Fragments arranged by size:
4211 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2749 2749
Size: 7213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2241 2321 2614 2915 3278 3427 3574
Size: 393 609 80 293 301 363 149 147
Cuts at: 3574 3844 4330 5276 5873 6660 6928 1239
Size: 270 486 946 597 787 268 1524
Fragments arranged by size:
1524 946 787 609 597 486 393 363
301 293 270 268 149 147 80
EcoNI CCTnn'n_nnAGG
Cuts at: 3869 3869
Size: 7213
EcoO109I rG'GnC_Cy
Cuts at: 2145 3478 3684 3703 4105 4106 4350 4351 2145
Size: 1333 206 19 402 1 244 1 5007
Fragments arranged by size:
5007 1333 402 244 206 19 1 1
EcoRI G'AATT_C
Cuts at: 4522 4900 4522
Size: 378 6835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3910 3910
Size: 7213
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 7213
FspI TGC'GCA
Cuts at: 5719 5719
Size: 7213
HaeII r_GCGC'y
Cuts at: 1678 6360 1678
Size: 4682 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 2288 2320 3498
Size: 32 135 32 565 32 119 32 1178
Cuts at: 3498 3530 4020 4052 4178 4210 5854 5886 5928
Size: 32 490 32 126 32 1644 32 42
Cuts at: 5928 5960 1373
Size: 32 2626
Fragments arranged by size:
2626 1644 1178 565 490 135 126 119
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3046 3919 4557 4803 669
Size: 988 281 1108 873 638 246 3079
Fragments arranged by size:
3079 1108 988 873 638 281 246
HindIII A'AGCT_T
Cuts at: 748 2088 3892 748
Size: 1340 1804 4069
Fragments arranged by size:
4069 1804 1340
HpaI GTT'AAC
Cuts at: 4803 4803
Size: 7213
KpnI G_GTAC'C
Cuts at: 4538 4538
Size: 7213
MfeI C'AATT_G
Cuts at: 4812 4812
Size: 7213
MluI A'CGCG_T
Cuts at: 7089 7089
Size: 7213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 3031 3601 6327 6511 1305
Size: 1539 187 570 2726 184 2007
Fragments arranged by size:
2726 2007 1539 570 187 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3458 4370 10
Size: 54 1425 343 1626 912 2853
Fragments arranged by size:
2853 1626 1425 912 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3223 3545 3722 3740 4203 5171 5530
Size: 648 2057 322 177 18 463 968 359
Cuts at: 5530 5689 518
Size: 159 2042
Fragments arranged by size:
2057 2042 968 648 463 359 322 177
159 18
MspA1I CmG'CkG
Cuts at: 1606 2496 3838 4022 4112 4257 4629 5306 6454
Size: 890 1342 184 90 145 372 677 1148
Cuts at: 6454 6699 1606
Size: 245 2120
Fragments arranged by size:
2120 1342 1148 890 677 372 245 184
145 90
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 7213
NcoI C'CATG_G
Cuts at: 513 1065 2004 2600 4224 4308 4497 513
Size: 552 939 596 1624 84 189 3229
Fragments arranged by size:
3229 1624 939 596 552 189 84
NdeI CA'TA_TG
Cuts at: 387 4340 387
Size: 3953 3260
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 7213
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7213
NotI GC'GGCC_GC
Cuts at: 4660 4660
Size: 7213
NspI r_CATG'y
Cuts at: 1132 1229 4571 6116 1132
Size: 97 3342 1545 2229
Fragments arranged by size:
3342 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7213
PciI A'CATG_T
Cuts at: 6112 6112
Size: 7213
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3001 1108
Size: 1893 5320
PfoI T'CCnGG_A
Cuts at: 2555 3344 3728 3828 2555
Size: 789 384 100 5940
Fragments arranged by size:
5940 789 384 100
PmeI GTTT'AAAC
Cuts at: 4517 4517
Size: 7213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5282 5314 6795 6827 5282
Size: 32 1481 32 5668
Fragments arranged by size:
5668 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2145 3478 3684 2145
Size: 1333 206 5674
Fragments arranged by size:
5674 1333 206
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7213
PsiI TTA'TAA
Cuts at: 4783 4783
Size: 7213
PspOMI G'GGCC_C
Cuts at: 4105 4350 4105
Size: 245 6968
PstI C_TGCA'G
Cuts at: 830 2424 2540 2991 3155 3408 3935 4272 4565
Size: 1594 116 451 164 253 527 337 293
Cuts at: 4565 830
Size: 3478
Fragments arranged by size:
3478 1594 527 451 337 293 253 164
116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7213
RsrII CG'GwC_CG
Cuts at: 2453 2453
Size: 7213
SacI G_AGCT'C
Cuts at: 2751 2751
Size: 7213
SalI G'TCGA_C
Cuts at: 1936 4555 1936
Size: 2619 4594
SanDI GG'GwC_CC
Cuts at: 2145 2145
Size: 7213
SapI GCTCTTCn'nnn_
Cuts at: 3971 3971
Size: 7213
SbfI CC_TGCA'GG
Cuts at: 4565 4565
Size: 7213
ScaI AGT'ACT
Cuts at: 5461 7173 5461
Size: 1712 5501
SexAI A'CCwGG_T
Cuts at: 3753 3753
Size: 7213
SfcI C'TryA_G
Cuts at: 826 1045 2420 2536 2987 3151 3404 3931 4244
Size: 219 1375 116 451 164 253 527 313
Cuts at: 4244 4268 4561 5696 6377 6568 826
Size: 24 293 1135 681 191 1471
Fragments arranged by size:
1471 1375 1135 681 527 451 313 293
253 219 191 164 116 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7213
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7213
SmaI CCC'GGG
Cuts at: 1872 2862 3504 3845 4540 1872
Size: 990 642 341 695 4545
Fragments arranged by size:
4545 990 695 642 341
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3049 5317 6218 6480 820
Size: 197 926 1106 2268 901 262 1553
Fragments arranged by size:
2268 1553 1106 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5004 493
Size: 4511 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7213
SphI G_CATG'C
Cuts at: 1132 4571 1132
Size: 3439 3774
SspI AAT'ATT
Cuts at: 5 52 2293 5137 5
Size: 47 2241 2844 2081
Fragments arranged by size:
2844 2241 2081 47
StuI AGG'CCT
Cuts at: 1517 3587 1517
Size: 2070 5143
StyI C'CwwG_G
Cuts at: 513 1065 2004 2600 3363 3986 4224 4308 4497
Size: 552 939 596 763 623 238 84 189
Cuts at: 4497 4652 513
Size: 155 3074
Fragments arranged by size:
3074 939 763 623 596 552 238 189
155 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2048 2441 4348 5240 5257 5410 5595
Size: 1255 468 393 1907 892 17 153 185
Cuts at: 5595 6040 325
Size: 445 1498
Fragments arranged by size:
1907 1498 1255 892 468 445 393 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3483 5459 7171
Size: 274 80 33 51 1443 1506 1976 1712
Cuts at: 7171 96
Size: 138
Fragments arranged by size:
1976 1712 1506 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 4456 4488 7173 7205 3179
Size: 32 1245 32 2685 32 3187
Fragments arranged by size:
3187 2685 1245 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2477 3607 1316
Size: 1161 1130 4922
Fragments arranged by size:
4922 1161 1130
XbaI T'CTAG_A
Cuts at: 3136 4549 3136
Size: 1413 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3754 4439 3754
Size: 685 6528
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7213
XmaI C'CCGG_G
Cuts at: 1870 2860 3502 3843 4538 1870
Size: 990 642 341 695 4545
Fragments arranged by size:
4545 990 695 642 341
XmnI GAAnn'nnTTC
Cuts at: 5342 5342
Size: 7213
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5021 276
Size: 53 83 186 936 3487 2468
Fragments arranged by size:
3487 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI
BstXI BstZ17I DraIII EcoRV FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI PvuII SacII SfiI
SfoI SrfI SwaI