Restriction map for
pFN21AE0386
(Circular) (Six-base) MAPSORT of: pFN21AE0386.seq Check: 9946 from: 1 to: 6787
pFN21AE0386 6787 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3057 3057
Size: 6787
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4597 278
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2388 2670 4130 1937
Size: 451 282 1460 4594
Fragments arranged by size:
4594 1460 451 282
Acc65I G'GTAC_C
Cuts at: 4081 4108 4081
Size: 27 6760
AclI AA'CG_TT
Cuts at: 1563 4914 5287 1563
Size: 3351 373 3063
Fragments arranged by size:
3351 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3689 3743 4850 6234 6502 1632
Size: 2057 54 1107 1384 268 1917
Fragments arranged by size:
2057 1917 1384 1107 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6590
AflIII A'CryG_T
Cuts at: 1203 5686 6663 1203
Size: 4483 977 1327
Fragments arranged by size:
4483 1327 977
AgeI A'CCGG_T
Cuts at: 5618 5618
Size: 6787
AhdI GACnn_n'nnGTC
Cuts at: 5516 5516
Size: 6787
AleI CACnn'nnGTG
Cuts at: 1166 2348 2897 1166
Size: 1182 549 5056
Fragments arranged by size:
5056 1182 549
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2366 2987 1184
Size: 1182 621 4984
Fragments arranged by size:
4984 1182 621
AlwNI CAG_nnn'CTG
Cuts at: 2876 3531 3613 4530 6102 2876
Size: 655 82 917 1572 3561
Fragments arranged by size:
3561 1572 917 655 82
ApaI G_GGCC'C
Cuts at: 2322 2721 3909 2322
Size: 399 1188 5200
Fragments arranged by size:
5200 1188 399
ApaLI G'TGCA_C
Cuts at: 3976 4844 6000 3976
Size: 868 1156 4763
Fragments arranged by size:
4763 1156 868
ApoI r'AATT_y
Cuts at: 1493 2807 4096 4322 4474 6583 1493
Size: 1314 1289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1314 1289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5341 160
Size: 872 4309 1606
Fragments arranged by size:
4309 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
AvaI C'yCGr_G
Cuts at: 1870 1943 2750 4112 1870
Size: 73 807 1362 4545
Fragments arranged by size:
4545 1362 807 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2680 2713 2680
Size: 33 6754
BamHI G'GATC_C
Cuts at: 3888 4117 4484 3888
Size: 229 367 6191
Fragments arranged by size:
6191 367 229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2471 2941 4051 4081 4108
Size: 325 217 627 684 470 1110 30 27
Cuts at: 4108 5563 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1110 684 627 470 325 217
30 27
BanII G_rGCy'C
Cuts at: 1391 2322 2721 3191 3417 3909 3963 4106 1391
Size: 931 399 470 226 492 54 143 4072
Fragments arranged by size:
4072 931 492 470 399 226 143 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2914 3731 928
Size: 971 1015 817 3984
Fragments arranged by size:
3984 1015 971 817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4978 5012 1903
Size: 34 3041 34 3678
Fragments arranged by size:
3678 3041 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4679 5889 4679
Size: 1210 5577
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6787
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3904 5398 136
Size: 107 122 71 631 605 2232 1494 1525
Fragments arranged by size:
2232 1525 1494 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6782 6782
Size: 6787
BlpI GC'TnA_GC
Cuts at: 4204 4204
Size: 6787
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2128 2322 2345 2721 3909 3980 4056
Size: 306 32 194 23 376 1188 71 76
Cuts at: 4056 4848 6004 1790
Size: 792 1156 2573
Fragments arranged by size:
2573 1188 1156 792 376 306 194 76
71 32 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4518 5476
Size: 416 53 83 399 24 2102 999 958
Cuts at: 5476 442
Size: 1753
Fragments arranged by size:
2102 1753 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3436 3398
Size: 38 6749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2677 2969 3059 3977 5447 1239
Size: 1438 292 90 918 1470 2579
Fragments arranged by size:
2579 1470 1438 918 292 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 2838 4912 5777 6075 2540
Size: 298 2074 865 298 3252
Fragments arranged by size:
3252 2074 865 298 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2305 2380 5450 882
Size: 617 806 75 3070 2219
Fragments arranged by size:
3070 2219 806 617 75
BsaAI yAC'GTr
Cuts at: 493 1204 2247 3445 3688 4578 493
Size: 711 1043 1198 243 890 2702
Fragments arranged by size:
2702 1198 1043 890 711 243
BsaBI GATnn'nnATC
Cuts at: 6781 6781
Size: 6787
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2214 4594 4976 5611
Size: 53 83 186 936 681 2380 382 635
Cuts at: 5611 275
Size: 1451
Fragments arranged by size:
2380 1451 936 681 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5220 5618 5892 6039 6450 5220
Size: 398 274 147 411 5557
Fragments arranged by size:
5557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3091 3121 1178
Size: 30 165 30 1688 30 4844
Fragments arranged by size:
4844 1688 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2738 2861 1186
Size: 1552 123 5112
Fragments arranged by size:
5112 1552 123
BseYI C'CCAG_C
Cuts at: 2273 2459 2600 2713 3304 3338 3390 3733 3768
Size: 186 141 113 591 34 52 343 35
Cuts at: 3768 5990 6486 2273
Size: 2222 496 2574
Fragments arranged by size:
2574 2222 591 496 343 186 141 113
52 35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2983 1163
Size: 573 1247 4967
Fragments arranged by size:
4967 1247 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4237 4998 6026 1540
Size: 275 186 2236 761 1028 2301
Fragments arranged by size:
2301 2236 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3417 3980 4848 4933 6004 3417
Size: 563 868 85 1071 4200
Fragments arranged by size:
4200 1071 868 563 85
BsiWI C'GTAC_G
Cuts at: 2247 2247
Size: 6787
BsmI GAATG_Cn'
Cuts at: 2792 3699 4298 4391 2792
Size: 907 599 93 5188
Fragments arranged by size:
5188 907 599 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2128 2322 2345 2721 3191 3417
Size: 399 306 32 194 23 376 470 226
Cuts at: 3417 3909 3963 3980 4056 4106 4848 4933 6004
Size: 492 54 17 76 50 742 85 1071
Cuts at: 6004 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 492 470 399 376 306
226 194 85 76 54 50 32 23
17
BspEI T'CCGG_A
Cuts at: 6450 6450
Size: 6787
BspHI T'CATG_A
Cuts at: 4676 6406 4676
Size: 1730 5057
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3057 3385 3932 4142 844
Size: 2213 328 547 210 3489
Fragments arranged by size:
3489 2213 547 328 210
BsrBI CCG'CTC
Cuts at: 1394 1823 4674 1394
Size: 429 2851 3507
Fragments arranged by size:
3507 2851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2407 5282 5456 65
Size: 1183 1159 2875 174 1396
Fragments arranged by size:
2875 1396 1183 1159 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3406 5431 5618 1700
Size: 110 1596 2025 187 2869
Fragments arranged by size:
2869 2025 1596 187 110
BsrGI T'GTAC_A
Cuts at: 96 2883 96
Size: 2787 4000
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6787
BssSI C'ACGA_G
Cuts at: 4847 5859 4847
Size: 1012 5775
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6787
BstBI TT'CG_AA
Cuts at: 5591 5591
Size: 6787
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6787
BstXI CCAn_nnnn'nTGG
Cuts at: 2162 3061 2162
Size: 899 5888
BstYI r'GATC_y
Cuts at: 1972 2110 2448 3888 4117 4484 4869 4886 6338
Size: 138 338 1440 229 367 385 17 1452
Cuts at: 6338 6424 6436 6782 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1440 385 367 346 338 229
138 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3144 3144
Size: 6787
BtgI C'CryG_G
Cuts at: 513 1065 2004 2155 2283 2337 513
Size: 552 939 151 128 54 4963
Fragments arranged by size:
4963 939 552 151 128 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2253 487
Size: 683 56 1027 5021
Fragments arranged by size:
5021 1027 683 56
BtsI GCAGTG_nn'
Cuts at: 4303 5097 5117 4303
Size: 794 20 5973
Fragments arranged by size:
5973 794 20
ClaI AT'CG_AT
Cuts at: 1554 2178 1554
Size: 624 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6752
DraI TTT'AAA
Cuts at: 4091 4091
Size: 6787
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2069 5794 809
Size: 507 753 3725 1802
Fragments arranged by size:
3725 1802 753 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2184 3234 3439
Size: 54 1425 50 275 18 354 1050 205
Cuts at: 3439 3516 4234 5123 6752 8
Size: 77 718 889 1629 43
Fragments arranged by size:
1629 1425 1050 889 718 354 275 205
77 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4234 1537
Size: 275 2422 4090
Fragments arranged by size:
4090 2422 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2643 3011 3585 4717 1353
Size: 1290 368 574 1132 3423
Fragments arranged by size:
3423 1290 1132 574 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4034 5374 5748 5894 387
Size: 1064 138 2445 1340 374 146 1280
Fragments arranged by size:
2445 1340 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3415 3415
Size: 6787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2677 2969 3059 3689 3743 3977 4850
Size: 393 1045 292 90 630 54 234 873
Cuts at: 4850 5447 6234 6502 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1045 873 787 630 597 393 292
268 234 90 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2843 3832 4010 2843
Size: 989 178 5620
Fragments arranged by size:
5620 989 178
EcoO109I rG'GnC_Cy
Cuts at: 2456 2597 2717 3049 3825 3906 3921 2456
Size: 141 120 332 776 81 15 5322
Fragments arranged by size:
5322 776 332 141 120 81 15
EcoRI G'AATT_C
Cuts at: 2807 4096 4474 2807
Size: 1289 378 5120
Fragments arranged by size:
5120 1289 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3844 3997 3844
Size: 153 6634
FspI TGC'GCA
Cuts at: 5293 5293
Size: 6787
HaeII r_GCGC'y
Cuts at: 1678 3407 5934 1678
Size: 1729 2527 2531
Fragments arranged by size:
2531 2527 1729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2440 2472 5428
Size: 32 135 32 713 32 123 32 2956
Cuts at: 5428 5460 5502 5534 1373
Size: 32 42 32 2626
Fragments arranged by size:
2956 2626 713 135 123 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4131 4377 669
Size: 988 281 2193 246 3079
Fragments arranged by size:
3079 2193 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6787
HpaI GTT'AAC
Cuts at: 4377 4377
Size: 6787
KpnI G_GTAC'C
Cuts at: 4085 4112 4085
Size: 27 6760
MfeI C'AATT_G
Cuts at: 4386 4386
Size: 6787
MluI A'CGCG_T
Cuts at: 6663 6663
Size: 6787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3997 5901 6085 1305
Size: 2692 1904 184 2007
Fragments arranged by size:
2692 2007 1904 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2186 3236 3441 3518 10
Size: 54 1425 343 354 1050 205 77 3279
Fragments arranged by size:
3279 1425 1050 354 343 205 77 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2897 3447 4745 5104 5263 518
Size: 648 1182 549 550 1298 359 159 2042
Fragments arranged by size:
2042 1298 1182 648 550 549 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2348 2463 2604 2977 3304 3772 4203
Size: 512 230 115 141 373 327 468 431
Cuts at: 4203 4880 6028 6273 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 468 431 373 327
245 230 141 115
NcoI C'CATG_G
Cuts at: 513 1065 2004 2155 513
Size: 552 939 151 5145
Fragments arranged by size:
5145 939 552 151
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6787
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6787
NotI GC'GGCC_GC
Cuts at: 4234 4234
Size: 6787
NspI r_CATG'y
Cuts at: 1132 1229 4145 5690 1132
Size: 97 2916 1545 2229
Fragments arranged by size:
2916 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3964 1348
Size: 2616 4171
PciI A'CATG_T
Cuts at: 5686 5686
Size: 6787
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2223 2226 2466 3232 3652 1108
Size: 1115 3 240 766 420 4243
Fragments arranged by size:
4243 1115 766 420 240 3
PfoI T'CCnGG_A
Cuts at: 3075 3210 3075
Size: 135 6652
PmeI GTTT'AAAC
Cuts at: 4091 4091
Size: 6787
PmlI CAC'GTG
Cuts at: 3445 3445
Size: 6787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4856 4888 6369 6401 4856
Size: 32 1481 32 5242
Fragments arranged by size:
5242 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2456 3049 3825 3921 2456
Size: 593 776 96 5322
Fragments arranged by size:
5322 776 593 96
PshAI GACnn'nnGTC
Cuts at: 1613 4013 1613
Size: 2400 4387
PsiI TTA'TAA
Cuts at: 4357 4357
Size: 6787
PspOMI G'GGCC_C
Cuts at: 2318 2717 3905 2318
Size: 399 1188 5200
Fragments arranged by size:
5200 1188 399
PstI C_TGCA'G
Cuts at: 830 2976 2982 4139 830
Size: 2146 6 1157 3478
Fragments arranged by size:
3478 2146 1157 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6787
PvuII CAG'CTG
Cuts at: 2348 2604 2977 3772 2348
Size: 256 373 795 5363
Fragments arranged by size:
5363 795 373 256
SacI G_AGCT'C
Cuts at: 3417 3417
Size: 6787
SalI G'TCGA_C
Cuts at: 1936 4129 1936
Size: 2193 4594
SanDI GG'GwC_CC
Cuts at: 2456 2456
Size: 6787
SapI GCTCTTCn'nnn_
Cuts at: 3011 3585 3011
Size: 574 6213
SbfI CC_TGCA'GG
Cuts at: 4139 4139
Size: 6787
ScaI AGT'ACT
Cuts at: 3179 5035 6747 3179
Size: 1856 1712 3219
Fragments arranged by size:
3219 1856 1712
SfcI C'TryA_G
Cuts at: 826 1045 2787 2972 2978 4135 5270 5951 6142
Size: 219 1742 185 6 1157 1135 681 191
Cuts at: 6142 826
Size: 1471
Fragments arranged by size:
1742 1471 1157 1135 681 219 191 185
6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6787
SgrAI Cr'CCGG_yG
Cuts at: 1700 3406 1700
Size: 1706 5081
SmaI CCC'GGG
Cuts at: 1872 4114 1872
Size: 2242 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 2853 4891 5792 6054 820
Size: 197 926 576 334 2038 901 262 1553
Fragments arranged by size:
2038 1553 926 901 576 334 262 197
SnaBI TAC'GTA
Cuts at: 493 4578 493
Size: 4085 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6787
SphI G_CATG'C
Cuts at: 1132 4145 1132
Size: 3013 3774
SspI AAT'ATT
Cuts at: 5 52 4711 5
Size: 47 4659 2081
Fragments arranged by size:
4659 2081 47
StuI AGG'CCT
Cuts at: 1517 2817 1517
Size: 1300 5487
StyI C'CwwG_G
Cuts at: 513 1065 2004 2155 2593 2643 2739 3286 4226
Size: 552 939 151 438 50 96 547 940
Cuts at: 4226 513
Size: 3074
Fragments arranged by size:
3074 940 939 552 547 438 151 96
50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2293 3116 4814 4831 4984 5169 5614
Size: 1255 713 823 1698 17 153 185 445
Cuts at: 5614 325
Size: 1498
Fragments arranged by size:
1698 1498 1255 823 713 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2883 3177 5033
Size: 274 80 33 51 1443 906 294 1856
Cuts at: 5033 6745 96
Size: 1712 138
Fragments arranged by size:
1856 1712 1443 906 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3091 3123 6747 6779 3091
Size: 32 3624 32 3099
Fragments arranged by size:
3624 3099 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3121 1316
Size: 1805 4982
XbaI T'CTAG_A
Cuts at: 2380 4123 2380
Size: 1743 5044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2281 2011
Size: 270 6517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6787
XmaI C'CCGG_G
Cuts at: 1870 4112 1870
Size: 2242 4545
XmnI GAAnn'nnTTC
Cuts at: 4916 4916
Size: 6787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4595 276
Size: 53 83 186 936 3061 2468
Fragments arranged by size:
3061 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI