Restriction map for
pFN21AE0384
(Circular) (Six-base) MAPSORT of: pFN21AE0384.seq Check: 9855 from: 1 to: 6172
pFN21AE0384 6172 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2790 3982 278
Size: 53 83 186 936 1254 1192 2468
Fragments arranged by size:
2468 1254 1192 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3113 4235 5619 5887 1632
Size: 1481 1122 1384 268 1917
Fragments arranged by size:
1917 1481 1384 1122 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2883 5071 6048 1203
Size: 1680 2188 977 1327
Fragments arranged by size:
2188 1680 1327 977
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2960 1184
Size: 1776 4396
AlwNI CAG_nnn'CTG
Cuts at: 2645 2698 2710 3346 3915 5487 2645
Size: 53 12 636 569 1572 3330
Fragments arranged by size:
3330 1572 636 569 53 12
ApaI G_GGCC'C
Cuts at: 2135 2184 2238 2293 2493 2520 2135
Size: 49 54 55 200 27 5787
Fragments arranged by size:
5787 200 55 54 49 27
ApaLI G'TGCA_C
Cuts at: 2257 4229 5385 2257
Size: 1972 1156 3044
Fragments arranged by size:
3044 1972 1156
ApoI r'AATT_y
Cuts at: 1493 3481 3707 3859 5968 1493
Size: 1988 226 152 2109 1697
Fragments arranged by size:
2109 1988 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 2128 2195 2231 2912 2999 3497
Size: 73 167 18 67 36 681 87 498
Cuts at: 3497 1870
Size: 4545
Fragments arranged by size:
4545 681 498 167 87 73 67 36
18
BamHI G'GATC_C
Cuts at: 3225 3502 3869 3225
Size: 277 367 5528
Fragments arranged by size:
5528 367 277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2087 2114 2200 2483
Size: 325 217 627 234 66 27 86 283
Cuts at: 2483 2963 2975 3108 3204 3493 4948 618
Size: 480 12 133 96 289 1455 1842
Fragments arranged by size:
1842 1455 627 480 325 289 283 234
217 133 96 86 66 27 12
BanII G_rGCy'C
Cuts at: 1391 2135 2184 2238 2293 2433 2493 2520 2919
Size: 744 49 54 55 140 60 27 399
Cuts at: 2919 3045 3182 3491 1391
Size: 126 137 309 4072
Fragments arranged by size:
4072 744 399 309 140 137 126 60
55 54 49 27
BbeI G_GCGC'C
Cuts at: 2091 2118 2487 2091
Size: 27 369 5776
Fragments arranged by size:
5776 369 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3405 928
Size: 971 1506 3695
Fragments arranged by size:
3695 1506 971
BbvCI CC'TCA_GC
Cuts at: 2738 2827 2738
Size: 89 6083
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4363 4397 1903
Size: 34 50 34 2342 34 3678
Fragments arranged by size:
3678 2342 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3244 4064 5274 3244
Size: 820 1210 4142
Fragments arranged by size:
4142 1210 820
BclI T'GATC_A
Cuts at: 1548 3057 1548
Size: 1509 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2066 2233
Size: 107 122 71 631 605 337 57 167
Cuts at: 2233 2481 3281 4783 136
Size: 248 800 1502 1525
Fragments arranged by size:
1525 1502 800 631 605 337 248 167
122 107 71 57
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 2808 3589 2808
Size: 781 5391
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2135 2184 2203 2238 2261 2293 2493
Size: 262 83 49 19 35 23 32 200
Cuts at: 2493 2520 2655 2978 3000 4233 5389 1790
Size: 27 135 323 22 1233 1156 2573
Fragments arranged by size:
2573 1233 1156 323 262 200 135 83
49 35 32 27 23 22 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2633 3298 3903
Size: 416 53 83 399 24 1216 665 605
Cuts at: 3903 4861 442
Size: 958 1753
Fragments arranged by size:
1753 1216 958 665 605 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2564 2570 2612 2705 2738 2826 2834
Size: 1223 102 6 42 93 33 88 8
Cuts at: 2834 4832 1239
Size: 1998 2579
Fragments arranged by size:
2579 1998 1223 102 93 88 42 33
8 6
Bpu10I CC'TnA_GC
Cuts at: 2147 2738 2827 3086 2147
Size: 591 89 259 5233
Fragments arranged by size:
5233 591 259 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 2861 3230 6166 2861
Size: 369 2936 2867
Fragments arranged by size:
2936 2867 369
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2115 2484 2556
Size: 53 83 186 936 555 27 369 72
Cuts at: 2556 2787 3979 4361 4996 275
Size: 231 1192 382 635 1451
Fragments arranged by size:
1451 1192 936 635 555 382 369 231
186 83 72 53 27
BsaWI w'CCGG_w
Cuts at: 3182 4605 5003 5277 5424 5835 3182
Size: 1423 398 274 147 411 3519
Fragments arranged by size:
3519 1423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2807 2837 1178
Size: 30 165 30 1404 30 4513
Fragments arranged by size:
4513 1404 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6172
BseYI C'CCAG_C
Cuts at: 2292 2443 2632 3075 3376 5375 5871 2292
Size: 151 189 443 301 1999 496 2593
Fragments arranged by size:
2593 1999 496 443 301 189 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2141 3346 1163
Size: 573 405 1205 3989
Fragments arranged by size:
3989 1205 573 405
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 2361 2587 3622 4383 5411
Size: 275 186 105 255 226 1035 761 1028
Cuts at: 5411 1540
Size: 2301
Fragments arranged by size:
2301 1035 1028 761 275 255 226 186
105
BsiHKAI G_wGCw'C
Cuts at: 2261 2357 2433 2820 2919 4233 4318 5389 2261
Size: 96 76 387 99 1314 85 1071 3044
Fragments arranged by size:
3044 1314 1071 387 99 96 85 76
BsmI GAATG_Cn'
Cuts at: 2924 3683 3776 2924
Size: 759 93 5320
Fragments arranged by size:
5320 759 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2135 2184 2203 2238 2261 2293
Size: 399 262 83 49 19 35 23 32
Cuts at: 2293 2357 2433 2493 2520 2655 2820 2919 2978
Size: 64 76 60 27 135 165 99 59
Cuts at: 2978 3000 3045 3182 3491 4233 4318 5389 1391
Size: 22 45 137 309 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 262 165 137
135 99 85 83 76 64 60 59
49 45 35 32 27 23 22 19
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3527 844
Size: 2683 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2460 4059 1394
Size: 429 637 1599 3507
Fragments arranged by size:
3507 1599 637 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3297 3351 4667 4841 65
Size: 1183 2049 54 1316 174 1396
Fragments arranged by size:
2049 1396 1316 1183 174 54
BsrFI r'CCGG_y
Cuts at: 1700 1810 2435 2657 2754 2966 3264 4816 5003
Size: 110 625 222 97 212 298 1552 187
Cuts at: 5003 1700
Size: 2869
Fragments arranged by size:
2869 1552 625 298 222 212 187 110
97
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 2151 2214 2396 2563 1925
Size: 226 63 182 167 5534
Fragments arranged by size:
5534 226 182 167 63
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2224 794
Size: 1430 4742
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstYI r'GATC_y
Cuts at: 1972 3225 3502 3869 4254 4271 5723 5809 5821
Size: 1253 277 367 385 17 1452 86 12
Cuts at: 5821 6167 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1253 385 367 346 277 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2205 2505 3045 513
Size: 552 1140 300 540 3640
Fragments arranged by size:
3640 1140 552 540 300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 3106 487
Size: 683 56 797 1083 3553
Fragments arranged by size:
3553 1083 797 683 56
BtsI GCAGTG_nn'
Cuts at: 3363 3371 3688 4482 4502 3363
Size: 8 317 794 20 5033
Fragments arranged by size:
5033 794 317 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5179 809
Size: 507 3863 1802
Fragments arranged by size:
3863 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2208 2358
Size: 54 1425 50 275 18 228 150 150
Cuts at: 2358 2508 2968 3262 3619 4508 6137 8
Size: 150 460 294 357 889 1629 43
Fragments arranged by size:
1629 1425 889 460 357 294 275 228
150 150 150 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2358 3619 1537
Size: 275 546 1261 4090
Fragments arranged by size:
4090 1261 546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2328 4102 1353
Size: 975 1774 3423
Fragments arranged by size:
3423 1774 975
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2917 2431
Size: 486 5686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2564 2570 2612 2705 2738 2826
Size: 393 830 102 6 42 93 33 88
Cuts at: 2826 2834 3113 4235 4832 5619 5887 1239
Size: 8 279 1122 597 787 268 1524
Fragments arranged by size:
1524 1122 830 787 597 393 279 268
102 93 88 42 33 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2594 2594
Size: 6172
EcoO109I rG'GnC_Cy
Cuts at: 2181 2235 2289 2490 2517 2823 3343 2181
Size: 54 54 201 27 306 520 5010
Fragments arranged by size:
5010 520 306 201 54 54 27
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3457 3457
Size: 6172
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2078 2091 2118 2316 2384 2487 5319 1678
Size: 400 13 27 198 68 103 2832 2531
Fragments arranged by size:
2832 2531 400 198 103 68 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2807 2839 4813 4845 4887
Size: 32 135 32 1235 32 1974 32 42
Cuts at: 4887 4919 1373
Size: 32 2626
Fragments arranged by size:
2626 1974 1235 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 2087 2114 2483 2087
Size: 27 369 5776
Fragments arranged by size:
5776 369 27
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 5286 5470 1305
Size: 1458 2523 184 2007
Fragments arranged by size:
2523 2007 1458 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4350
Fragments arranged by size:
4350 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4130 4489 4648 518
Size: 648 2964 359 159 2042
Fragments arranged by size:
2964 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2140 2207 2224 2266 2368 2451 2627 2683
Size: 534 67 17 42 102 83 176 56
Cuts at: 2683 2695 2961 3079 3588 4265 5413 5658 1606
Size: 12 266 118 509 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 534 509 266 245 176
118 102 83 67 56 42 17 12
NaeI GCC'GGC
Cuts at: 2756 2756
Size: 6172
NarI GG'CG_CC
Cuts at: 2088 2115 2484 2088
Size: 27 369 5776
Fragments arranged by size:
5776 369 27
NcoI C'CATG_G
Cuts at: 513 1065 2505 3045 513
Size: 552 1440 540 3640
Fragments arranged by size:
3640 1440 552 540
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 2754 2754
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2887 2941 3530 5075 1132
Size: 97 1658 54 589 1545 2229
Fragments arranged by size:
2229 1658 1545 589 97 54
PasI CC'CwG_GG
Cuts at: 1348 2520 3256 3393 1348
Size: 1172 736 137 4127
Fragments arranged by size:
4127 1172 736 137
PciI A'CATG_T
Cuts at: 2883 5071 2883
Size: 2188 3984
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 3346 1108
Size: 1602 636 3934
Fragments arranged by size:
3934 1602 636
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2823 3343 2823
Size: 520 5652
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 2131 2180 2234 2289 2489 2516 2131
Size: 49 54 55 200 27 5787
Fragments arranged by size:
5787 200 55 54 49 27
PstI C_TGCA'G
Cuts at: 830 2352 2388 2796 3177 3524 830
Size: 1522 36 408 381 347 3478
Fragments arranged by size:
3478 1522 408 381 347 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2368 2627 2683 2695 3079 2368
Size: 259 56 12 384 5461
Fragments arranged by size:
5461 384 259 56 12
SacI G_AGCT'C
Cuts at: 2433 2919 2433
Size: 486 5686
SacII CC_GC'GG
Cuts at: 2208 2208
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SapI GCTCTTCn'nnn_
Cuts at: 2328 2328
Size: 6172
SbfI CC_TGCA'GG
Cuts at: 2796 3524 2796
Size: 728 5444
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 2348 2384 2792 2833 3173 3520 4655
Size: 219 1303 36 408 41 340 347 1135
Cuts at: 4655 5336 5527 826
Size: 681 191 1471
Fragments arranged by size:
1471 1303 1135 681 408 347 340 219
191 41 36
SfoI GGC'GCC
Cuts at: 2089 2116 2485 2089
Size: 27 369 5776
Fragments arranged by size:
5776 369 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 2130 2197 2233 3001 3499 1872
Size: 258 67 36 768 498 4545
Fragments arranged by size:
4545 768 498 258 67 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4276 5177 5439 820
Size: 197 926 2333 901 262 1553
Fragments arranged by size:
2333 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SrfI GCCC'GGGC
Cuts at: 2233 3001 2233
Size: 768 5404
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 3284 3416 1517
Size: 1767 132 4273
Fragments arranged by size:
4273 1767 132
StyI C'CwwG_G
Cuts at: 513 1065 2505 3045 3465 3611 513
Size: 552 1440 540 420 146 3074
Fragments arranged by size:
3074 1440 552 540 420 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3248 3293 4199 4216 4369 4554
Size: 1255 512 1156 45 906 17 153 185
Cuts at: 4554 4999 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1156 906 512 445 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3019 4418 6130
Size: 274 80 33 51 1443 1042 1399 1712
Cuts at: 6130 96
Size: 138
Fragments arranged by size:
1712 1443 1399 1042 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2050
Size: 6172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 2128 2195 2231 2999 3497 1870
Size: 258 67 36 768 498 4545
Fragments arranged by size:
4545 768 498 258 67 36
XmnI GAAnn'nnTTC
Cuts at: 4301 4301
Size: 6172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2788 3980 276
Size: 53 83 186 936 1254 1192 2468
Fragments arranged by size:
2468 1254 1192 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SanDI SexAI SfiI SwaI