Restriction map for
pFN21AE0383
(Circular) (Six-base) MAPSORT of: pFN21AE0383.seq Check: 4984 from: 1 to: 7849
pFN21AE0383 7849 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3485 3485
Size: 7849
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5659 278
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2605 5192 1937
Size: 668 2587 4594
Fragments arranged by size:
4594 2587 668
Acc65I G'GTAC_C
Cuts at: 5170 5170
Size: 7849
AclI AA'CG_TT
Cuts at: 1563 5976 6349 1563
Size: 4413 373 3063
Fragments arranged by size:
4413 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3971 4065 4832 4868 5912 7296 7564 1632
Size: 2339 94 767 36 1044 1384 268 1917
Fragments arranged by size:
2339 1917 1384 1044 767 268 94 36
AfeI AGC'GCT
Cuts at: 2714 2714
Size: 7849
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7652
AflIII A'CryG_T
Cuts at: 1203 4008 4306 6748 7725 1203
Size: 2805 298 2442 977 1327
Fragments arranged by size:
2805 2442 1327 977 298
AgeI A'CCGG_T
Cuts at: 6680 6680
Size: 7849
AhdI GACnn_n'nnGTC
Cuts at: 2815 6578 2815
Size: 3763 4086
AleI CACnn'nnGTG
Cuts at: 1166 3278 1166
Size: 2112 5737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2990 3053 4082 1184
Size: 1806 63 1029 4951
Fragments arranged by size:
4951 1806 1029 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3711 3743 3711
Size: 32 7817
AlwNI CAG_nnn'CTG
Cuts at: 3625 4285 4705 4876 5592 7164 3625
Size: 660 420 171 716 1572 4310
Fragments arranged by size:
4310 1572 716 660 420 171
ApaLI G'TGCA_C
Cuts at: 5906 7062 5906
Size: 1156 6693
ApoI r'AATT_y
Cuts at: 1493 3539 3589 4413 5158 5384 5536 7645 1493
Size: 2046 50 824 745 226 152 2109 1697
Fragments arranged by size:
2109 2046 1697 824 745 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 5142 6403 160
Size: 872 4110 1261 1606
Fragments arranged by size:
4110 1606 1261 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
AvaI C'yCGr_G
Cuts at: 1870 1943 5174 1870
Size: 73 3231 4545
Fragments arranged by size:
4545 3231 73
BamHI G'GATC_C
Cuts at: 5179 5546 5179
Size: 367 7482
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 4188 4365 5170 6625
Size: 325 217 627 417 1984 177 805 1455
Cuts at: 6625 618
Size: 1842
Fragments arranged by size:
1984 1842 1455 805 627 417 325 217
177
BanII G_rGCy'C
Cuts at: 1391 2196 2638 2877 5168 1391
Size: 805 442 239 2291 4072
Fragments arranged by size:
4072 2291 805 442 239
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 3510 4398 928
Size: 971 1023 588 888 4379
Fragments arranged by size:
4379 1023 971 888 588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6040 6074 1903
Size: 34 4103 34 3678
Fragments arranged by size:
4103 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5741 6951 5741
Size: 1210 6639
BclI T'GATC_A
Cuts at: 1548 2997 1548
Size: 1449 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2246 6460 136
Size: 107 122 71 631 605 574 4214 1525
Fragments arranged by size:
4214 1525 631 605 574 122 107 71
BglII A'GATC_T
Cuts at: 3421 7844 3421
Size: 4423 3426
BlpI GC'TnA_GC
Cuts at: 2963 4019 5266 2963
Size: 1056 1247 5546
Fragments arranged by size:
5546 1247 1056
Bme1580I G_kGCm'C
Cuts at: 1790 5910 7066 1790
Size: 4120 1156 2573
Fragments arranged by size:
4120 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2014 1318
Size: 696 7153
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5580 6538 442
Size: 416 53 83 399 24 4163 958 1753
Fragments arranged by size:
4163 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7849
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4209 4710 5035 4209
Size: 501 325 7023
Fragments arranged by size:
7023 501 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2921 2941 4009 4508 4547 4898 6509
Size: 1211 471 20 1068 499 39 351 1611
Cuts at: 6509 1239
Size: 2579
Fragments arranged by size:
2579 1611 1211 1068 499 471 351 39
20
Bpu10I CC'TnA_GC
Cuts at: 4619 4619
Size: 7849
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3163 3446 3925 4987 5005 5974 6839 7137 3163
Size: 283 479 1062 18 969 865 298 3875
Fragments arranged by size:
3875 1062 969 865 479 298 283 18
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3126 6512 882
Size: 617 1627 3386 2219
Fragments arranged by size:
3386 2219 1627 617
BsaAI yAC'GTr
Cuts at: 493 1204 3610 5640 493
Size: 711 2406 2030 2702
Fragments arranged by size:
2702 2406 2030 711
BsaBI GATnn'nnATC
Cuts at: 7843 7843
Size: 7849
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5656 6038 6673 275
Size: 53 83 186 936 4123 382 635 1451
Fragments arranged by size:
4123 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3288 6282 6680 6954 7101 7512 3288
Size: 2994 398 274 147 411 3625
Fragments arranged by size:
3625 2994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 3123 3153 4520
Size: 30 165 30 687 30 1003 30 1367
Cuts at: 4520 4550 1178
Size: 30 4477
Fragments arranged by size:
4477 1367 1003 687 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2171 2576 2885 3140 5085 1186
Size: 858 127 405 309 255 1945 3950
Fragments arranged by size:
3950 1945 858 405 309 255 127
BseYI C'CCAG_C
Cuts at: 2244 4521 7052 7548 2244
Size: 2277 2531 496 2545
Fragments arranged by size:
2545 2531 2277 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3714 4179 5299 6060 7088 1540
Size: 275 186 1713 465 1120 761 1028 2301
Fragments arranged by size:
2301 1713 1120 1028 761 465 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 2638 2877 5910 5995 7066 2196
Size: 442 239 3033 85 1071 2979
Fragments arranged by size:
3033 2979 1071 442 239 85
BsmI GAATG_Cn'
Cuts at: 4675 4981 5360 5453 4675
Size: 306 379 93 7071
Fragments arranged by size:
7071 379 306 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2638 2877 5168 5910 5995 7066
Size: 399 406 442 239 2291 742 85 1071
Cuts at: 7066 1391
Size: 2174
Fragments arranged by size:
2291 2174 1071 742 442 406 399 239
85
BspEI T'CCGG_A
Cuts at: 7512 7512
Size: 7849
BspHI T'CATG_A
Cuts at: 3000 4002 5738 7468 3000
Size: 1002 1736 1730 3381
Fragments arranged by size:
3381 1736 1730 1002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 5204 844
Size: 2641 1719 3489
Fragments arranged by size:
3489 2641 1719
BsrBI CCG'CTC
Cuts at: 1394 1823 5736 1394
Size: 429 3913 3507
Fragments arranged by size:
3913 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2734 4066 6344 6518 65
Size: 1183 1486 1332 2278 174 1396
Fragments arranged by size:
2278 1486 1396 1332 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6493 6680 1700
Size: 110 4683 187 2869
Fragments arranged by size:
4683 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2133 2548 4862 96
Size: 2037 415 2314 3083
Fragments arranged by size:
3083 2314 2037 415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7849
BssSI C'ACGA_G
Cuts at: 2558 2813 3524 5909 6921 2558
Size: 255 711 2385 1012 3486
Fragments arranged by size:
3486 2385 1012 711 255
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2932 794
Size: 2138 5711
BstBI TT'CG_AA
Cuts at: 2309 6653 2309
Size: 4344 3505
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7849
BstXI CCAn_nnnn'nTGG
Cuts at: 2620 2620
Size: 7849
BstYI r'GATC_y
Cuts at: 1972 2801 3339 3421 4355 4872 5179 5546 5931
Size: 829 538 82 934 517 307 367 385
Cuts at: 5931 5948 7400 7486 7498 7844 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 934 829 538 517 385 367
346 307 86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 4220 4220
Size: 7849
BtgI C'CryG_G
Cuts at: 513 1065 2619 3153 3714 4920 513
Size: 552 1554 534 561 1206 3442
Fragments arranged by size:
3442 1554 1206 561 552 534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7110
Fragments arranged by size:
7110 683 56
BtsI GCAGTG_nn'
Cuts at: 3820 4382 5365 6159 6179 3820
Size: 562 983 794 20 5490
Fragments arranged by size:
5490 983 794 562 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7849
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7814
DraI TTT'AAA
Cuts at: 5153 5153
Size: 7849
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3512 6856 809
Size: 507 2196 3344 1802
Fragments arranged by size:
3344 2196 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2622 2661 3375
Size: 54 1425 50 275 18 792 39 714
Cuts at: 3375 3711 4176 4401 5296 6185 7814 8
Size: 336 465 225 895 889 1629 43
Fragments arranged by size:
1629 1425 895 889 792 714 465 336
275 225 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3711 4176 5296 1537
Size: 275 1899 465 1120 4090
Fragments arranged by size:
4090 1899 1120 465 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3176 5027 5034 5779 1353
Size: 1823 1851 7 745 3423
Fragments arranged by size:
3423 1851 1823 745 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2067 6436 6810 6956 387
Size: 1064 138 478 4369 374 146 1280
Fragments arranged by size:
4369 1280 1064 478 374 146 138
EcoICRI GAG'CTC
Cuts at: 2194 2636 2875 2194
Size: 442 239 7168
Fragments arranged by size:
7168 442 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2450 2921 2941 3971 4009 4065 4508
Size: 393 818 471 20 1030 38 56 443
Cuts at: 4508 4547 4832 4868 4898 5912 6509 7296 7564
Size: 39 285 36 30 1014 597 787 268
Cuts at: 7564 1239
Size: 1524
Fragments arranged by size:
1524 1030 1014 818 787 597 471 443
393 285 268 56 39 38 36 30
20
EcoNI CCTnn'n_nnAGG
Cuts at: 4591 4591
Size: 7849
EcoO109I rG'GnC_Cy
Cuts at: 2809 2905 3187 3820 4151 4843 2809
Size: 96 282 633 331 692 5815
Fragments arranged by size:
5815 692 633 331 282 96
EcoRI G'AATT_C
Cuts at: 5158 5536 5158
Size: 378 7471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 3440 4741 2197
Size: 1243 1301 5305
Fragments arranged by size:
5305 1301 1243
FspI TGC'GCA
Cuts at: 6355 6355
Size: 7849
HaeII r_GCGC'y
Cuts at: 1678 2716 3625 4052 6996 1678
Size: 1038 909 427 2944 2531
Fragments arranged by size:
2944 2531 1038 909 427
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 2864 2896 3123
Size: 32 135 32 1229 32 31 32 227
Cuts at: 3123 3155 3619 3651 4076 4108 4520 4552 4739
Size: 32 464 32 425 32 412 32 187
Cuts at: 4739 4771 5028 5060 5117 5149 6490 6522 6564
Size: 32 257 32 57 32 1341 32 42
Cuts at: 6564 6596 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 1229 464 425 412 257 227
187 135 57 42 32 32 32 32
32 32 32 32 32 32 32 32
32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 5193 5439 669
Size: 988 281 3255 246 3079
Fragments arranged by size:
3255 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4334 748
Size: 3586 4263
HpaI GTT'AAC
Cuts at: 5439 5439
Size: 7849
KpnI G_GTAC'C
Cuts at: 5174 5174
Size: 7849
MfeI C'AATT_G
Cuts at: 5448 5448
Size: 7849
MluI A'CGCG_T
Cuts at: 7725 7725
Size: 7849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4368 6963 7147 1305
Size: 3063 2595 184 2007
Fragments arranged by size:
3063 2595 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 2663 4403 10
Size: 54 1425 343 792 39 1740 3456
Fragments arranged by size:
3456 1740 1425 792 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 3259 3278 3730 3792 4007 5807 6166
Size: 648 2093 19 452 62 215 1800 359
Cuts at: 6166 6325 518
Size: 159 2042
Fragments arranged by size:
2093 2042 1800 648 452 359 215 159
62 19
MspA1I CmG'CkG
Cuts at: 1606 2244 4525 5265 5942 7090 7335 1606
Size: 638 2281 740 677 1148 245 2120
Fragments arranged by size:
2281 2120 1148 740 677 638 245
NcoI C'CATG_G
Cuts at: 513 1065 3153 4920 513
Size: 552 2088 1767 3442
Fragments arranged by size:
3442 2088 1767 552
NdeI CA'TA_TG
Cuts at: 387 2495 387
Size: 2108 5741
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7849
NotI GC'GGCC_GC
Cuts at: 4176 5296 4176
Size: 1120 6729
NspI r_CATG'y
Cuts at: 1132 1229 3030 3391 4310 5207 6752 1132
Size: 97 1801 361 919 897 1545 2229
Fragments arranged by size:
2229 1801 1545 919 897 361 97
PasI CC'CwG_GG
Cuts at: 1348 2599 1348
Size: 1251 6598
PciI A'CATG_T
Cuts at: 4306 6748 4306
Size: 2442 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2220 4921 1108
Size: 1112 2701 4036
Fragments arranged by size:
4036 2701 1112
PfoI T'CCnGG_A
Cuts at: 3852 4875 3852
Size: 1023 6826
PmeI GTTT'AAAC
Cuts at: 5153 5153
Size: 7849
PmlI CAC'GTG
Cuts at: 3610 3610
Size: 7849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5918 5950 7431 7463 5918
Size: 32 1481 32 6304
Fragments arranged by size:
6304 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 2905 3187 3820 2809
Size: 96 282 633 6838
Fragments arranged by size:
6838 633 282 96
PshAI GACnn'nnGTC
Cuts at: 1613 3452 1613
Size: 1839 6010
PsiI TTA'TAA
Cuts at: 5419 5419
Size: 7849
PstI C_TGCA'G
Cuts at: 830 4377 4701 5201 830
Size: 3547 324 500 3478
Fragments arranged by size:
3547 3478 500 324
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7849
PvuII CAG'CTG
Cuts at: 2244 4525 2244
Size: 2281 5568
SacI G_AGCT'C
Cuts at: 2196 2638 2877 2196
Size: 442 239 7168
Fragments arranged by size:
7168 442 239
SalI G'TCGA_C
Cuts at: 1936 5191 1936
Size: 3255 4594
SanDI GG'GwC_CC
Cuts at: 3187 3820 3187
Size: 633 7216
SbfI CC_TGCA'GG
Cuts at: 5201 5201
Size: 7849
ScaI AGT'ACT
Cuts at: 2200 2567 6097 7809 2200
Size: 367 3530 1712 2240
Fragments arranged by size:
3530 2240 1712 367
SexAI A'CCwGG_T
Cuts at: 2226 2226
Size: 7849
SfcI C'TryA_G
Cuts at: 826 1045 4373 4697 5197 6332 7013 7204 826
Size: 219 3328 324 500 1135 681 191 1471
Fragments arranged by size:
3328 1471 1135 681 500 324 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7849
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7849
SmaI CCC'GGG
Cuts at: 1872 5176 1872
Size: 3304 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3142 3425 3904 4966 5020 5953
Size: 197 926 1199 283 479 1062 54 933
Cuts at: 5953 6854 7116 820
Size: 901 262 1553
Fragments arranged by size:
1553 1199 1062 933 926 901 479 283
262 197 54
SnaBI TAC'GTA
Cuts at: 493 5640 493
Size: 5147 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7849
SphI G_CATG'C
Cuts at: 1132 5207 1132
Size: 4075 3774
SspI AAT'ATT
Cuts at: 5 52 3965 5773 5
Size: 47 3913 1808 2081
Fragments arranged by size:
3913 2081 1808 47
StuI AGG'CCT
Cuts at: 1517 3701 5015 1517
Size: 2184 1314 4351
Fragments arranged by size:
4351 2184 1314
StyI C'CwwG_G
Cuts at: 513 1065 2385 2542 3153 3466 4920 5288 513
Size: 552 1320 157 611 313 1454 368 3074
Fragments arranged by size:
3074 1454 1320 611 552 368 313 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3394 5115 5876 5893 6046 6231 6676
Size: 1255 1814 1721 761 17 153 185 445
Cuts at: 6676 325
Size: 1498
Fragments arranged by size:
1814 1721 1498 1255 761 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 2198 2548
Size: 274 80 33 51 1443 156 65 350
Cuts at: 2548 2565 4862 6095 7807 96
Size: 17 2297 1233 1712 138
Fragments arranged by size:
2297 1712 1443 1233 350 274 156 138
80 65 51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2023 2055 3350 3382 7809 7841 2023
Size: 32 1295 32 4427 32 2031
Fragments arranged by size:
4427 2031 1295 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 3137 1316
Size: 912 909 6028
Fragments arranged by size:
6028 912 909
XbaI T'CTAG_A
Cuts at: 5185 5185
Size: 7849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3110 4144 3110
Size: 1034 6815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7849
XmaI C'CCGG_G
Cuts at: 1870 5174 1870
Size: 3304 4545
XmnI GAAnn'nnTTC
Cuts at: 5978 5978
Size: 7849
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5657 276
Size: 53 83 186 936 4123 2468
Fragments arranged by size:
4123 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacII SapI SfiI SfoI SrfI SwaI