Restriction map for
pFN21AE0381
(Circular) (Six-base) MAPSORT of: pFN21AE0381.seq Check: 4234 from: 1 to: 6244
pFN21AE0381 6244 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 2222 2241 2934 3565 2222
Size: 19 693 631 4901
Fragments arranged by size:
4901 693 631 19
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4307 5691 5959 1632
Size: 2675 1384 268 1917
Fragments arranged by size:
2675 1917 1384 268
AfeI AGC'GCT
Cuts at: 3074 3074
Size: 6244
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 5143 6120 1203
Size: 3940 977 1327
Fragments arranged by size:
3940 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2551 4973 2551
Size: 2422 3822
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3432 3464 3432
Size: 32 6212
AlwNI CAG_nnn'CTG
Cuts at: 3040 3987 5559 3040
Size: 947 1572 3725
Fragments arranged by size:
3725 1572 947
ApaI G_GGCC'C
Cuts at: 3315 3315
Size: 6244
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 3553 3779 3931 6040 1493
Size: 2060 226 152 2109 1697
Fragments arranged by size:
2109 2060 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2199 3569 1870
Size: 73 180 76 1370 4545
Fragments arranged by size:
4545 1370 180 76 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2222 2241 2934 3089 3267
Size: 325 217 627 435 19 693 155 178
Cuts at: 3267 3344 3565 5020 618
Size: 77 221 1455 1842
Fragments arranged by size:
1842 1455 693 627 435 325 221 217
178 155 77 19
BanII G_rGCy'C
Cuts at: 1391 2149 2505 2586 2640 3315 3462 3563 1391
Size: 758 356 81 54 675 147 101 4072
Fragments arranged by size:
4072 758 675 356 147 101 81 54
BbeI G_GCGC'C
Cuts at: 3271 3348 3271
Size: 77 6167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5273
BbvCI CC'TCA_GC
Cuts at: 3099 3386 3099
Size: 287 5957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2119 3466 4855
Size: 107 122 71 631 605 447 1347 1389
Cuts at: 4855 136
Size: 1525
Fragments arranged by size:
1525 1389 1347 631 605 447 122 107
71
BglII A'GATC_T
Cuts at: 2784 2805 6239 2784
Size: 21 3434 2789
Fragments arranged by size:
3434 2789 21
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 3315 3434 4305 5461 1790
Size: 1525 119 871 1156 2573
Fragments arranged by size:
2573 1525 1156 871 119
BmgBI CAC'GTC
Cuts at: 1318 2689 2857 1318
Size: 1371 168 4705
Fragments arranged by size:
4705 1371 168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2258 3975 4933
Size: 416 53 83 399 24 841 1717 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1717 958 841 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 2194 2345 2447 2468 2579 2627 2741
Size: 778 177 151 102 21 111 48 114
Cuts at: 2741 2780 3179 3296 4904 1239
Size: 39 399 117 1608 2579
Fragments arranged by size:
2579 1608 778 399 177 151 117 114
111 102 48 39 21
Bpu10I CC'TnA_GC
Cuts at: 3099 3386 3099
Size: 287 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3104 3188 4369 5234 5532 3104
Size: 84 1181 865 298 3816
Fragments arranged by size:
3816 1181 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 2717 2807 4907 882
Size: 617 945 273 90 2100 2219
Fragments arranged by size:
2219 2100 945 617 273 90
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3268 3345 3396 4051
Size: 53 83 186 936 1735 77 51 655
Cuts at: 4051 4433 5068 275
Size: 382 635 1451
Fragments arranged by size:
1735 1451 936 655 635 382 186 83
77 53 51
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2441 2471 1178
Size: 30 165 30 1038 30 4951
Fragments arranged by size:
4951 1038 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 2792 2795 2966 3176 1186
Size: 1462 144 3 171 210 4254
Fragments arranged by size:
4254 1462 210 171 144 3
BseYI C'CCAG_C
Cuts at: 2969 3069 3235 3515 3530 5447 5943 2969
Size: 100 166 280 15 1917 496 3270
Fragments arranged by size:
3270 1917 496 280 166 100 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2363 2586 2765 1163
Size: 573 627 223 179 4642
Fragments arranged by size:
4642 627 573 223 179
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 2586 2640 3291 4305 4390 5461 2149
Size: 437 54 651 1014 85 1071 2932
Fragments arranged by size:
2932 1071 1014 651 437 85 54
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2149 2505 2586 2640 3291 3315 3434
Size: 399 359 356 81 54 651 24 119
Cuts at: 3434 3462 3563 4305 4390 5461 1391
Size: 28 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 651 399 359 356 119
101 85 81 54 28 24
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3599 844
Size: 2755 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 4131 1394
Size: 429 1663 645 3507
Fragments arranged by size:
3507 1663 645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3415 4739 4913 65
Size: 1183 2167 1324 174 1396
Fragments arranged by size:
2167 1396 1324 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 2943 4888 5075 1700
Size: 110 308 825 1945 187 2869
Fragments arranged by size:
2869 1945 825 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 2741 4304 5316 2741
Size: 1563 1012 3669
Fragments arranged by size:
3669 1563 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6244
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 2086 1184
Size: 902 5342
BstYI r'GATC_y
Cuts at: 1972 2784 2805 3009 3574 3941 4326 4343 5795
Size: 812 21 204 565 367 385 17 1452
Cuts at: 5795 5881 5893 6239 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 812 565 385 367 346 204
86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 3401 3401
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2854 2884 487
Size: 683 56 1628 30 3847
Fragments arranged by size:
3847 1628 683 56 30
BtsI GCAGTG_nn'
Cuts at: 2106 3760 4554 4574 2106
Size: 1654 794 20 3776
Fragments arranged by size:
3776 1654 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3691 4580 6209
Size: 54 1425 50 275 18 1861 889 1629
Cuts at: 6209 8
Size: 43
Fragments arranged by size:
1861 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 3279 4174 1353
Size: 1272 654 895 3423
Fragments arranged by size:
3423 1272 895 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3505 4831 5205 5351 387
Size: 1064 138 1916 1326 374 146 1280
Fragments arranged by size:
1916 1326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2147 2584 2638 2147
Size: 437 54 5753
Fragments arranged by size:
5753 437 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2194 2345 2447 2468 2579 2627
Size: 393 385 177 151 102 21 111 48
Cuts at: 2627 2741 2780 3179 3296 4307 4904 5691 5959
Size: 114 39 399 117 1011 597 787 268
Cuts at: 5959 1239
Size: 1524
Fragments arranged by size:
1524 1011 787 597 399 393 385 268
177 151 117 114 111 102 48 39
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 2774 3203 3275 2708
Size: 66 429 72 5677
Fragments arranged by size:
5677 429 72 66
EcoO109I rG'GnC_Cy
Cuts at: 2137 2196 2137
Size: 59 6185
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 6244
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 2529 3076 3271 3348 5391 1678
Size: 851 547 195 77 2043 2531
Fragments arranged by size:
2531 2043 851 547 195 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 4885 4917 4959
Size: 32 135 32 965 32 2316 32 42
Cuts at: 4959 4991 1373
Size: 32 2626
Fragments arranged by size:
2626 2316 965 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 3267 3344 3267
Size: 77 6167
KpnI G_GTAC'C
Cuts at: 2226 2245 2938 3569 2226
Size: 19 693 631 4901
Fragments arranged by size:
4901 693 631 19
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5358 5542 1305
Size: 4053 184 2007
Fragments arranged by size:
4053 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4422
Fragments arranged by size:
4422 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3540 4202 4561 4720 518
Size: 648 2374 662 359 159 2042
Fragments arranged by size:
2374 2042 662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2542 2969 3037 3660 4337 5485 5730 1606
Size: 936 427 68 623 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 677 623 427 245 68
NaeI GCC'GGC
Cuts at: 2120 2120
Size: 6244
NarI GG'CG_CC
Cuts at: 3268 3345 3268
Size: 77 6167
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5692
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NgoMIV G'CCGG_C
Cuts at: 2118 2118
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 3545 3602 5147 1132
Size: 97 2316 57 1545 2229
Fragments arranged by size:
2316 2229 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 3463 1348
Size: 2115 4129
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6244
PfoI T'CCnGG_A
Cuts at: 2424 3012 2424
Size: 588 5656
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2819 1613
Size: 1206 5038
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 3311 3311
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2591 3045 3174 3422 3596 830
Size: 1761 454 129 248 174 3478
Fragments arranged by size:
3478 1761 454 248 174 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2542 2969 2542
Size: 427 5817
SacI G_AGCT'C
Cuts at: 2149 2586 2640 2149
Size: 437 54 5753
Fragments arranged by size:
5753 437 54
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SapI GCTCTTCn'nnn_
Cuts at: 3279 3279
Size: 6244
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2587 3041 3170 3262 3418 3592 4727
Size: 219 1542 454 129 92 156 174 1135
Cuts at: 4727 5408 5599 826
Size: 681 191 1471
Fragments arranged by size:
1542 1471 1135 681 454 219 191 174
156 129 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 2119 2119
Size: 6244
SfoI GGC'GCC
Cuts at: 3269 3346 3269
Size: 77 6167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 2125 2201 3571 1872
Size: 253 76 1370 4545
Fragments arranged by size:
4545 1370 253 76
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3083 3203 4348 5249 5511 820
Size: 197 926 1140 120 1145 901 262 1553
Fragments arranged by size:
1553 1145 1140 926 901 262 197 120
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 1517
Size: 1413 4831
StyI C'CwwG_G
Cuts at: 513 1065 2158 3683 513
Size: 552 1093 1525 3074
Fragments arranged by size:
3074 1525 1093 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2105 2809 4271 4288 4441 4626 5071
Size: 1255 525 704 1462 17 153 185 445
Cuts at: 5071 325
Size: 1498
Fragments arranged by size:
1498 1462 1255 704 525 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4490 6202 96
Size: 274 80 33 51 1443 2513 1712 138
Fragments arranged by size:
2513 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2441 2473 2965 2997 6204 6236 2441
Size: 32 492 32 3207 32 2449
Fragments arranged by size:
3207 2449 492 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3145 3187 3238 3145
Size: 42 51 6151
Fragments arranged by size:
6151 51 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 2123 2199 3569 1870
Size: 253 76 1370 4545
Fragments arranged by size:
4545 1370 253 76
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SrfI SwaI