Restriction map for
pFN21AE0380
(Circular) (Six-base) MAPSORT of: pFN21AE0380.seq Check: 2146 from: 1 to: 6448
pFN21AE0380 6448 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4258 278
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3791 1937
Size: 1854 4594
Acc65I G'GTAC_C
Cuts at: 3769 3769
Size: 6448
AclI AA'CG_TT
Cuts at: 1563 4575 4948 1563
Size: 3012 373 3063
Fragments arranged by size:
3063 3012 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 3177 4511 5895 6163 1632
Size: 941 604 1334 1384 268 1917
Fragments arranged by size:
1917 1384 1334 941 604 268
AfeI AGC'GCT
Cuts at: 2363 2561 2363
Size: 198 6250
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6251
AflIII A'CryG_T
Cuts at: 1203 3474 5347 6324 1203
Size: 2271 1873 977 1327
Fragments arranged by size:
2271 1873 1327 977
AgeI A'CCGG_T
Cuts at: 5279 5279
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 3422 5177 3422
Size: 1755 4693
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2357 2779 1184
Size: 1173 422 4853
Fragments arranged by size:
4853 1173 422
AlwNI CAG_nnn'CTG
Cuts at: 2876 4191 5763 2876
Size: 1315 1572 3561
Fragments arranged by size:
3561 1572 1315
ApaLI G'TGCA_C
Cuts at: 4505 5661 4505
Size: 1156 5292
ApoI r'AATT_y
Cuts at: 1493 3026 3173 3757 3983 4135 6244 1493
Size: 1533 147 584 226 152 2109 1697
Fragments arranged by size:
2109 1697 1533 584 226 152 147
AseI AT'TA_AT
Cuts at: 160 1032 5002 160
Size: 872 3970 1606
Fragments arranged by size:
3970 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2357 3773 1870
Size: 73 234 180 1416 4545
Fragments arranged by size:
4545 1416 234 180 73
BamHI G'GATC_C
Cuts at: 2736 3778 4145 2736
Size: 1042 367 5039
Fragments arranged by size:
5039 1042 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 3769 5224 618
Size: 325 217 627 425 1557 1455 1842
Fragments arranged by size:
1842 1557 1455 627 425 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 2638 2826 3186 3767 1391
Size: 717 530 188 360 581 4072
Fragments arranged by size:
4072 717 581 530 360 188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5477
BbvCI CC'TCA_GC
Cuts at: 2324 2324
Size: 6448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3557 3591 4639 4673 1903
Size: 34 1620 34 1048 34 3678
Fragments arranged by size:
3678 1620 1048 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2883 3312 3538 4340 5550 2883
Size: 429 226 802 1210 3781
Fragments arranged by size:
3781 1210 802 429 226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6448
BfrBI ATG'CAT
Cuts at: 3559 3559
Size: 6448
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5059 136
Size: 107 122 71 631 605 3387 1525
Fragments arranged by size:
3387 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6443 6443
Size: 6448
BlpI GC'TnA_GC
Cuts at: 3865 3865
Size: 6448
Bme1580I G_kGCm'C
Cuts at: 1790 2768 3211 4509 5665 1790
Size: 978 443 1298 1156 2573
Fragments arranged by size:
2573 1298 1156 978 443
BmgBI CAC'GTC
Cuts at: 1318 3477 1318
Size: 2159 4289
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4179 5137 442
Size: 416 53 83 399 24 2762 958 1753
Fragments arranged by size:
2762 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3038
Size: 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 2866 3248 3335 5108 1239
Size: 1499 128 382 87 1773 2579
Fragments arranged by size:
2579 1773 1499 382 128 87
Bpu10I CC'TnA_GC
Cuts at: 2324 2324
Size: 6448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 4573 5438 5736 2504
Size: 2069 865 298 3216
Fragments arranged by size:
3216 2069 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2928 5111 882
Size: 617 1429 2183 2219
Fragments arranged by size:
2219 2183 1429 617
BsaAI yAC'GTr
Cuts at: 493 1204 2862 4239 493
Size: 711 1658 1377 2702
Fragments arranged by size:
2702 1658 1377 711
BsaBI GATnn'nnATC
Cuts at: 2315 6442 2315
Size: 4127 2321
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2651 4255 4637 5272
Size: 53 83 186 936 1118 1604 382 635
Cuts at: 5272 275
Size: 1451
Fragments arranged by size:
1604 1451 1118 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4881 5279 5553 5700 6111 4881
Size: 398 274 147 411 5218
Fragments arranged by size:
5218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2051 2081 3284 3314 1178
Size: 30 165 30 648 30 1203 30 4312
Fragments arranged by size:
4312 1203 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2303 2762 3027 1186
Size: 1099 18 459 265 4607
Fragments arranged by size:
4607 1099 459 265 18
BseYI C'CCAG_C
Cuts at: 2101 2227 2644 5651 6147 2101
Size: 126 417 3007 496 2402
Fragments arranged by size:
3007 2402 496 417 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2288 2327 3171 3685 1163
Size: 573 552 39 844 514 3926
Fragments arranged by size:
3926 844 573 552 514 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2056 2207 2332 3898 4659 5687
Size: 275 186 55 151 125 1566 761 1028
Cuts at: 5687 1540
Size: 2301
Fragments arranged by size:
2301 1566 1028 761 275 186 151 125
55
BsiHKAI G_wGCw'C
Cuts at: 2076 2550 3186 4509 4594 5665 2076
Size: 474 636 1323 85 1071 2859
Fragments arranged by size:
2859 1323 1071 636 474 85
BsmI GAATG_Cn'
Cuts at: 3959 4052 3959
Size: 93 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2108 2550 2638 2768 2826 3186
Size: 399 286 32 442 88 130 58 360
Cuts at: 3186 3211 3767 4509 4594 5665 1391
Size: 25 556 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 556 442 399 360 286
130 88 85 58 32 25
BspEI T'CCGG_A
Cuts at: 6111 6111
Size: 6448
BspHI T'CATG_A
Cuts at: 4337 6067 4337
Size: 1730 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3803 844
Size: 2959 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4335 1394
Size: 429 2512 3507
Fragments arranged by size:
3507 2512 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3522 4943 5117 65
Size: 1183 2274 1421 174 1396
Fragments arranged by size:
2274 1421 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2191 2209 2215 5092 5279 1700
Size: 110 381 18 6 2877 187 2869
Fragments arranged by size:
2877 2869 381 187 110 18 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6448
BssHII G'CGCG_C
Cuts at: 1925 2232 1925
Size: 307 6141
BssSI C'ACGA_G
Cuts at: 4508 5520 4508
Size: 1012 5436
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6448
BstBI TT'CG_AA
Cuts at: 2799 5252 2799
Size: 2453 3995
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6448
BstYI r'GATC_y
Cuts at: 1972 2736 3039 3614 3778 4145 4530 4547 5999
Size: 764 303 575 164 367 385 17 1452
Cuts at: 5999 6085 6097 6443 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 764 575 385 367 346 303
164 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3321 3513 3321
Size: 192 6256
BtgI C'CryG_G
Cuts at: 513 1065 2036 2201 513
Size: 552 971 165 4760
Fragments arranged by size:
4760 971 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2682 3179 487
Size: 683 56 1456 497 3756
Fragments arranged by size:
3756 1456 683 497 56
BtsI GCAGTG_nn'
Cuts at: 2339 2763 3053 3964 4758 4778 2339
Size: 424 290 911 794 20 4009
Fragments arranged by size:
4009 911 794 424 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6413
DraI TTT'AAA
Cuts at: 3178 3752 3178
Size: 574 5874
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5455 809
Size: 507 4139 1802
Fragments arranged by size:
4139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2094 2204
Size: 54 1425 50 275 18 223 41 110
Cuts at: 2204 2565 3088 3135 3895 4784 6413 8
Size: 361 523 47 760 889 1629 43
Fragments arranged by size:
1629 1425 889 760 523 361 275 223
110 54 50 47 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 2053 2204 3895 1537
Size: 275 241 151 1691 4090
Fragments arranged by size:
4090 1691 275 241 151
EarI CTCTTCn'nnn_
Cuts at: 1353 2739 4378 1353
Size: 1386 1639 3423
Fragments arranged by size:
3423 1639 1386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5035 5409 5555 387
Size: 1064 138 3446 374 146 1280
Fragments arranged by size:
3446 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3184 3184
Size: 6448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2573 2738 2866 3177 3248 3335 4511
Size: 393 941 165 128 311 71 87 1176
Cuts at: 4511 5108 5895 6163 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1176 941 787 597 393 311 268
165 128 87 71
EcoO109I rG'GnC_Cy
Cuts at: 2156 2623 2156
Size: 467 5981
EcoRI G'AATT_C
Cuts at: 3757 4135 3757
Size: 378 6070
EcoRV GAT'ATC
Cuts at: 2500 2500
Size: 6448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2537 2578 3304 3616 2537
Size: 41 726 312 5369
Fragments arranged by size:
5369 726 312 41
FspI TGC'GCA
Cuts at: 4954 4954
Size: 6448
HaeII r_GCGC'y
Cuts at: 1678 2032 2066 2084 2365 2563 5595 1678
Size: 354 34 18 281 198 3032 2531
Fragments arranged by size:
3032 2531 354 281 198 34 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3286 3318 3408
Size: 32 135 32 749 32 933 32 90
Cuts at: 3408 3440 3461 3493 3599 3631 5089 5121 5163
Size: 32 21 32 106 32 1458 32 42
Cuts at: 5163 5195 1373
Size: 32 2626
Fragments arranged by size:
2626 1458 933 749 135 106 90 42
32 32 32 32 32 32 32 32
32 21
HincII GTy'rAC
Cuts at: 669 1657 1938 2454 3792 4038 669
Size: 988 281 516 1338 246 3079
Fragments arranged by size:
3079 1338 988 516 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6448
HpaI GTT'AAC
Cuts at: 4038 4038
Size: 6448
KpnI G_GTAC'C
Cuts at: 3773 3773
Size: 6448
MfeI C'AATT_G
Cuts at: 4047 4047
Size: 6448
MluI A'CGCG_T
Cuts at: 6324 6324
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2951 5562 5746 1305
Size: 1646 2611 184 2007
Fragments arranged by size:
2611 2007 1646 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2567 3090 3137 10
Size: 54 1425 343 735 523 47 3321
Fragments arranged by size:
3321 1425 735 523 343 54 47
MslI CAynn'nnrTG
Cuts at: 518 1166 3073 3527 4406 4765 4924 518
Size: 648 1907 454 879 359 159 2042
Fragments arranged by size:
2042 1907 879 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2203 2260 2374 2725 3155 3864 4541
Size: 432 165 57 114 351 430 709 677
Cuts at: 4541 5689 5934 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 709 677 432 430 351 245
165 114 57
NaeI GCC'GGC
Cuts at: 2217 2217
Size: 6448
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5896
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6448
NgoMIV G'CCGG_C
Cuts at: 2215 2215
Size: 6448
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6448
NotI GC'GGCC_GC
Cuts at: 2204 3895 2204
Size: 1691 4757
NsiI A_TGCA'T
Cuts at: 3561 3561
Size: 6448
NspI r_CATG'y
Cuts at: 1132 1229 3317 3745 3806 5351 1132
Size: 97 2088 428 61 1545 2229
Fragments arranged by size:
2229 2088 1545 428 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6448
PciI A'CATG_T
Cuts at: 5347 5347
Size: 6448
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3412 1108
Size: 2304 4144
PfoI T'CCnGG_A
Cuts at: 2682 3252 2682
Size: 570 5878
PmeI GTTT'AAAC
Cuts at: 3752 3752
Size: 6448
PmlI CAC'GTG
Cuts at: 2862 2862
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4517 4549 6030 6062 4517
Size: 32 1481 32 4903
Fragments arranged by size:
4903 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2156 2156
Size: 6448
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6448
PsiI TTA'TAA
Cuts at: 2439 4018 2439
Size: 1579 4869
PstI C_TGCA'G
Cuts at: 830 2259 2265 2286 2733 3800 830
Size: 1429 6 21 447 1067 3478
Fragments arranged by size:
3478 1429 1067 447 21 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6448
PvuII CAG'CTG
Cuts at: 2260 3155 2260
Size: 895 5553
SacI G_AGCT'C
Cuts at: 3186 3186
Size: 6448
SacII CC_GC'GG
Cuts at: 2039 2204 2039
Size: 165 6283
SalI G'TCGA_C
Cuts at: 1936 3790 1936
Size: 1854 4594
SanDI GG'GwC_CC
Cuts at: 2156 2156
Size: 6448
SbfI CC_TGCA'GG
Cuts at: 3800 3800
Size: 6448
ScaI AGT'ACT
Cuts at: 4696 6408 4696
Size: 1712 4736
SexAI A'CCwGG_T
Cuts at: 3420 3420
Size: 6448
SfcI C'TryA_G
Cuts at: 826 1045 2255 2261 2282 2533 2729 3796 4931
Size: 219 1210 6 21 251 196 1067 1135
Cuts at: 4931 5612 5803 826
Size: 681 191 1471
Fragments arranged by size:
1471 1210 1135 1067 681 251 219 196
191 21 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
SgrAI Cr'CCGG_yG
Cuts at: 1700 2209 1700
Size: 509 5939
SmaI CCC'GGG
Cuts at: 1872 2179 3775 1872
Size: 307 1596 4545
Fragments arranged by size:
4545 1596 307
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 4552 5453 5715 820
Size: 197 926 540 2069 901 262 1553
Fragments arranged by size:
2069 1553 926 901 540 262 197
SnaBI TAC'GTA
Cuts at: 493 4239 493
Size: 3746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6448
SphI G_CATG'C
Cuts at: 1132 3806 1132
Size: 2674 3774
SspI AAT'ATT
Cuts at: 5 52 2966 4372 5
Size: 47 2914 1406 2081
Fragments arranged by size:
2914 2081 1406 47
StuI AGG'CCT
Cuts at: 1517 3331 1517
Size: 1814 4634
StyI C'CwwG_G
Cuts at: 513 1065 2589 3887 513
Size: 552 1524 1298 3074
Fragments arranged by size:
3074 1524 1298 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3087 3518 4475 4492 4645 4830 5275
Size: 1255 1507 431 957 17 153 185 445
Cuts at: 5275 325
Size: 1498
Fragments arranged by size:
1507 1498 1255 957 445 431 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3471 4694 6406
Size: 274 80 33 51 1443 1494 1223 1712
Cuts at: 6406 96
Size: 138
Fragments arranged by size:
1712 1494 1443 1223 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3062 3094 3617 3649 6408 6440 3062
Size: 32 523 32 2759 32 3070
Fragments arranged by size:
3070 2759 523 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6448
XbaI T'CTAG_A
Cuts at: 3784 3784
Size: 6448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6448
XmaI C'CCGG_G
Cuts at: 1870 2177 3773 1870
Size: 307 1596 4545
Fragments arranged by size:
4545 1596 307
XmnI GAAnn'nnTTC
Cuts at: 2795 3725 4577 2795
Size: 930 852 4666
Fragments arranged by size:
4666 930 852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4256 276
Size: 53 83 186 936 2722 2468
Fragments arranged by size:
2722 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI
NarI NruI PacI PspOMI PspXI PsrI RsrII SapI
SfiI SfoI SrfI SwaI XcmI