Restriction map for
pFN21AE0376
(Circular) (Six-base) MAPSORT of: pFN21AE0376.seq Check: 1460 from: 1 to: 6391
pFN21AE0376 6391 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 2202 3712 2202
Size: 1510 4881
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2762 3393 3456 4454 5838 6106 1632
Size: 1130 631 63 998 1384 268 1917
Fragments arranged by size:
1917 1384 1130 998 631 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 2954 3282 1166
Size: 1788 328 4275
Fragments arranged by size:
4275 1788 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2772 2804 2772
Size: 32 6359
AlwNI CAG_nnn'CTG
Cuts at: 4134 5706 4134
Size: 1572 4819
ApaLI G'TGCA_C
Cuts at: 2167 4448 5604 2167
Size: 2281 1156 2954
Fragments arranged by size:
2954 2281 1156
ApoI r'AATT_y
Cuts at: 1493 3362 3700 3926 4078 6187 1493
Size: 1869 338 226 152 2109 1697
Fragments arranged by size:
2109 1869 1697 338 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3716 1870
Size: 73 1773 4545
Fragments arranged by size:
4545 1773 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2629 2662 2629
Size: 33 6358
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2352 2431 3383 3712
Size: 325 217 627 415 150 79 952 329
Cuts at: 3712 5167 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 952 627 415 329 325 217
150 79
BanII G_rGCy'C
Cuts at: 1391 2994 3138 3710 1391
Size: 1603 144 572 4072
Fragments arranged by size:
4072 1603 572 144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2270 928
Size: 971 371 5049
Fragments arranged by size:
5049 971 371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4582 4616 1903
Size: 34 2645 34 3678
Fragments arranged by size:
3678 2645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3083 4283 5493 3083
Size: 1200 1210 3981
Fragments arranged by size:
3981 1210 1200
BclI T'GATC_A
Cuts at: 1548 2383 3126 3522 1548
Size: 835 743 396 4417
Fragments arranged by size:
4417 835 743 396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6386 6386
Size: 6391
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 2171 4452 5608 1790
Size: 381 2281 1156 2573
Fragments arranged by size:
2573 2281 1156 381
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4122 5080 442
Size: 416 53 83 399 24 2705 958 1753
Fragments arranged by size:
2705 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3008 5051 1239
Size: 1769 2043 2579
Fragments arranged by size:
2579 2043 1769
Bpu10I CC'TnA_GC
Cuts at: 2492 2492
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3445 4516 5381 5679 3445
Size: 1071 865 298 4157
Fragments arranged by size:
4157 1071 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3459 5054 882
Size: 617 1960 1595 2219
Fragments arranged by size:
2219 1960 1595 617
BsaAI yAC'GTr
Cuts at: 493 1204 2256 2812 4182 493
Size: 711 1052 556 1370 2702
Fragments arranged by size:
2702 1370 1052 711 556
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3248 4198 4580 5215
Size: 53 83 186 936 1715 950 382 635
Cuts at: 5215 275
Size: 1451
Fragments arranged by size:
1715 1451 950 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3093 4824 5222 5496 5643 6054 3093
Size: 1731 398 274 147 411 3430
Fragments arranged by size:
3430 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2772 2802 1178
Size: 30 165 30 1369 30 4767
Fragments arranged by size:
4767 1369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 2732 2780 3437 1186
Size: 1414 132 48 657 4140
Fragments arranged by size:
4140 1414 657 132 48
BseYI C'CCAG_C
Cuts at: 2142 3013 3116 5594 6090 2142
Size: 871 103 2478 496 2443
Fragments arranged by size:
2478 2443 871 496 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2792 3281 1163
Size: 573 729 327 489 4273
Fragments arranged by size:
4273 729 573 489 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2200 3841 4602 5630 1540
Size: 275 186 199 1641 761 1028 2301
Fragments arranged by size:
2301 1641 1028 761 275 199 186
BsiHKAI G_wGCw'C
Cuts at: 2099 2171 4452 4537 5608 2099
Size: 72 2281 85 1071 2882
Fragments arranged by size:
2882 2281 1071 85 72
BsmI GAATG_Cn'
Cuts at: 3902 3995 3902
Size: 93 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2099 2171 2994 3138 3710 4452 4537
Size: 399 309 72 823 144 572 742 85
Cuts at: 4537 5608 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 823 742 572 399 309 144
85 72
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2856 3746 844
Size: 2012 890 3489
Fragments arranged by size:
3489 2012 890
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 4278 1394
Size: 429 578 1877 3507
Fragments arranged by size:
3507 1877 578 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4886 5060 65
Size: 1183 3638 174 1396
Fragments arranged by size:
3638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 2199 5035 5222 1700
Size: 110 385 4 2836 187 2869
Fragments arranged by size:
2869 2836 385 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 3263 4451 5463 3263
Size: 1188 1012 4191
Fragments arranged by size:
4191 1188 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6391
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 3148 3284 3148
Size: 136 6255
BstYI r'GATC_y
Cuts at: 1972 2508 3030 3186 3721 4088 4473 4490 5942
Size: 536 522 156 535 367 385 17 1452
Cuts at: 5942 6028 6040 6386 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 536 535 522 385 367 346
156 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2320 2407 2514 2582 3614 3681 513
Size: 552 1255 87 107 68 1032 67 3223
Fragments arranged by size:
3223 1255 1032 552 107 87 68 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5652
Fragments arranged by size:
5652 683 56
BtsI GCAGTG_nn'
Cuts at: 3907 4701 4721 3907
Size: 794 20 5577
Fragments arranged by size:
5577 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 2940 3098
Size: 54 1425 50 275 18 367 743 158
Cuts at: 3098 3214 3838 4727 6356 8
Size: 116 624 889 1629 43
Fragments arranged by size:
1629 1425 889 743 624 367 275 158
116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2197 3838 1537
Size: 275 385 1641 4090
Fragments arranged by size:
4090 1641 385 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3278 4321 1353
Size: 1925 1043 3423
Fragments arranged by size:
3423 1925 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3195 4978 5352 5498 387
Size: 1064 138 1606 1783 374 146 1280
Fragments arranged by size:
1783 1606 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2762 3008 3393 3456 4454 5051 5838
Size: 393 1130 246 385 63 998 597 787
Cuts at: 5838 6106 1239
Size: 268 1524
Fragments arranged by size:
1524 1130 998 787 597 393 385 268
246 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2927
Size: 6391
EcoO109I rG'GnC_Cy
Cuts at: 3636 3636
Size: 6391
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 2799 3486 2611
Size: 188 687 5516
Fragments arranged by size:
5516 687 188
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2938 5538 1678
Size: 1260 2600 2531
Fragments arranged by size:
2600 2531 1260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5032 5064 5106 5138 1373
Size: 32 135 32 3460 32 42 32 2626
Fragments arranged by size:
3460 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3735 3981 669
Size: 988 281 1797 246 3079
Fragments arranged by size:
3079 1797 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 2206 3716 2206
Size: 1510 4881
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 3185 3282 5505 5689 1305
Size: 1340 540 97 2223 184 2007
Fragments arranged by size:
2223 2007 1340 540 184 97
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2942 10
Size: 54 1425 343 1110 3459
Fragments arranged by size:
3459 1425 1110 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 2954 3282 4349 4708 4867 518
Size: 648 1512 276 328 1067 359 159 2042
Fragments arranged by size:
2042 1512 1067 648 359 328 276 159
MspA1I CmG'CkG
Cuts at: 1606 2156 2487 2568 3013 3807 4484 5632 5877
Size: 550 331 81 445 794 677 1148 245
Cuts at: 5877 1606
Size: 2120
Fragments arranged by size:
2120 1148 794 677 550 445 331 245
81
NcoI C'CATG_G
Cuts at: 513 1065 2407 2582 3614 3681 513
Size: 552 1342 175 1032 67 3223
Fragments arranged by size:
3223 1342 1032 552 175 67
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3749 5294 1132
Size: 97 2520 1545 2229
Fragments arranged by size:
2520 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2953 1108
Size: 1845 4546
PfoI T'CCnGG_A
Cuts at: 2913 2913
Size: 6391
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PmlI CAC'GTG
Cuts at: 2256 2256
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2772 2804 4460 4492 5973 6005 2772
Size: 32 1656 32 1481 32 3158
Fragments arranged by size:
3158 1656 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3636 3636
Size: 6391
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 6359
PstI C_TGCA'G
Cuts at: 830 2316 3624 3743 830
Size: 1486 1308 119 3478
Fragments arranged by size:
3478 1486 1308 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2156 3013 2156
Size: 857 5534
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SfcI C'TryA_G
Cuts at: 826 1045 2312 3620 3739 4874 5555 5746 826
Size: 219 1267 1308 119 1135 681 191 1471
Fragments arranged by size:
1471 1308 1267 1135 681 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3460 4495 5396 5658 820
Size: 197 926 1517 1035 901 262 1553
Fragments arranged by size:
1553 1517 1035 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 2107 4315 5
Size: 47 2055 2208 2081
Fragments arranged by size:
2208 2081 2055 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2053 2407 2582 2952 3614 3681 3830
Size: 552 988 354 175 370 662 67 149
Cuts at: 3830 513
Size: 3074
Fragments arranged by size:
3074 988 662 552 370 354 175 149
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3005 3642 4418 4435 4588 4773 5218
Size: 1255 1425 637 776 17 153 185 445
Cuts at: 5218 325
Size: 1498
Fragments arranged by size:
1498 1425 1255 776 637 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 4637 6349
Size: 274 80 33 51 1443 981 1679 1712
Cuts at: 6349 96
Size: 138
Fragments arranged by size:
1712 1679 1443 981 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6351 6383 6351
Size: 32 6359
Tth111I GACn'n_nGTC
Cuts at: 1316 3166 1316
Size: 1850 4541
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2950 3145 2950
Size: 195 6196
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 2784 3406 3480 4520 2784
Size: 622 74 1040 4655
Fragments arranged by size:
4655 1040 622 74
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI