Restriction map for
pFN21AE0373
(Circular) (Six-base) MAPSORT of: pFN21AE0373.seq Check: 8059 from: 1 to: 6877
pFN21AE0373 6877 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4687 278
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4220 1937
Size: 2283 4594
Acc65I G'GTAC_C
Cuts at: 4198 4198
Size: 6877
AclI AA'CG_TT
Cuts at: 1563 5004 5377 1563
Size: 3441 373 3063
Fragments arranged by size:
3441 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2181 3165 4940 6324 6592 1632
Size: 503 46 984 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 984 503 268 46
AfeI AGC'GCT
Cuts at: 2303 2303
Size: 6877
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6680
AflIII A'CryG_T
Cuts at: 1203 3350 3926 5776 6753 1203
Size: 2147 576 1850 977 1327
Fragments arranged by size:
2147 1850 1327 977 576
AgeI A'CCGG_T
Cuts at: 2810 5708 2810
Size: 2898 3979
AhdI GACnn_n'nnGTC
Cuts at: 5606 5606
Size: 6877
AleI CACnn'nnGTG
Cuts at: 1166 4097 1166
Size: 2931 3946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6877
AlwNI CAG_nnn'CTG
Cuts at: 4620 6192 4620
Size: 1572 5305
ApaLI G'TGCA_C
Cuts at: 4934 6090 4934
Size: 1156 5721
ApoI r'AATT_y
Cuts at: 1493 2131 3036 3092 3133 3224 3312 3338 4186
Size: 638 905 56 41 91 88 26 848
Cuts at: 4186 4412 4564 6673 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 905 848 638 226 152 91
88 56 41 26
AseI AT'TA_AT
Cuts at: 160 1032 5431 160
Size: 872 4399 1606
Fragments arranged by size:
4399 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
AvaI C'yCGr_G
Cuts at: 1870 1943 4202 1870
Size: 73 2259 4545
Fragments arranged by size:
4545 2259 73
BamHI G'GATC_C
Cuts at: 4207 4574 4207
Size: 367 6510
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4198 5653 618
Size: 325 217 627 2411 1455 1842
Fragments arranged by size:
2411 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2169 4196 1391
Size: 778 2027 4072
Fragments arranged by size:
4072 2027 778
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2628 2689 2784 928
Size: 971 729 61 95 5021
Fragments arranged by size:
5021 971 729 95 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2084 2118 5068 5102 1903
Size: 34 147 34 2950 34 3678
Fragments arranged by size:
3678 2950 147 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4769 5979 4769
Size: 1210 5667
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2524 5488 136
Size: 107 122 71 631 605 852 2964 1525
Fragments arranged by size:
2964 1525 852 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6872 6872
Size: 6877
BlpI GC'TnA_GC
Cuts at: 4294 4294
Size: 6877
Bme1580I G_kGCm'C
Cuts at: 1790 2781 4938 6094 1790
Size: 991 2157 1156 2573
Fragments arranged by size:
2573 2157 1156 991
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2564 3596 4608
Size: 416 53 83 399 24 1147 1032 1012
Cuts at: 4608 5566 442
Size: 958 1753
Fragments arranged by size:
1753 1147 1032 1012 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6877
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3320 3320
Size: 6877
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 2282 2293 2337 3315 3408 3498 5537
Size: 793 250 11 44 978 93 90 2039
Cuts at: 5537 1239
Size: 2579
Fragments arranged by size:
2579 2039 978 793 250 93 90 44
11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5002 5867 6165 5002
Size: 865 298 5714
Fragments arranged by size:
5714 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2616 5540 882
Size: 617 1117 2924 2219
Fragments arranged by size:
2924 2219 1117 617
BsaAI yAC'GTr
Cuts at: 493 1204 4668 493
Size: 711 3464 2702
Fragments arranged by size:
3464 2702 711
BsaBI GATnn'nnATC
Cuts at: 3250 6871 3250
Size: 3621 3256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4684 5066 5701 275
Size: 53 83 186 936 3151 382 635 1451
Fragments arranged by size:
3151 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2229 2810 5310 5708 5982 6129 6540 2229
Size: 581 2500 398 274 147 411 2566
Fragments arranged by size:
2566 2500 581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 3267 3297 3546
Size: 30 165 30 1147 30 687 30 249
Cuts at: 3546 3576 1178
Size: 30 4479
Fragments arranged by size:
4479 1147 687 249 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2727 1186
Size: 1084 457 5336
Fragments arranged by size:
5336 1084 457
BseYI C'CCAG_C
Cuts at: 2719 6080 6576 2719
Size: 3361 496 3020
Fragments arranged by size:
3361 3020 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4327 5088 6116 1540
Size: 275 186 2326 761 1028 2301
Fragments arranged by size:
2326 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2563 4938 5023 6094 2563
Size: 2375 85 1071 3346
Fragments arranged by size:
3346 2375 1071 85
BsmI GAATG_Cn'
Cuts at: 3151 4123 4388 4481 3151
Size: 972 265 93 5547
Fragments arranged by size:
5547 972 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2563 2781 4196 4938 5023 6094
Size: 399 379 394 218 1415 742 85 1071
Cuts at: 6094 1391
Size: 2174
Fragments arranged by size:
2174 1415 1071 742 399 394 379 218
85
BspEI T'CCGG_A
Cuts at: 6540 6540
Size: 6877
BspHI T'CATG_A
Cuts at: 4766 6496 4766
Size: 1730 5147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4232 844
Size: 3388 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4764 1394
Size: 429 2941 3507
Fragments arranged by size:
3507 2941 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2573 5372 5546 65
Size: 1183 1325 2799 174 1396
Fragments arranged by size:
2799 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2810 3785 5521 5708 1700
Size: 110 1000 975 1736 187 2869
Fragments arranged by size:
2869 1736 1000 975 187 110
BsrGI T'GTAC_A
Cuts at: 96 2657 96
Size: 2561 4316
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6877
BssSI C'ACGA_G
Cuts at: 4937 5949 4937
Size: 1012 5865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6877
BstBI TT'CG_AA
Cuts at: 5681 5681
Size: 6877
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6877
BstYI r'GATC_y
Cuts at: 1972 2970 4150 4207 4574 4959 4976 6428 6514
Size: 998 1180 57 367 385 17 1452 86
Cuts at: 6514 6526 6872 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1180 998 385 367 346 86
57 17 12
Bsu36I CC'TnA_GG
Cuts at: 3812 3812
Size: 6877
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2944 487
Size: 683 56 1718 4420
Fragments arranged by size:
4420 1718 683 56
BtsI GCAGTG_nn'
Cuts at: 3672 4393 5187 5207 3672
Size: 721 794 20 5342
Fragments arranged by size:
5342 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6842
DraI TTT'AAA
Cuts at: 4181 4181
Size: 6877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5884 809
Size: 507 4568 1802
Fragments arranged by size:
4568 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2346 3783 4324
Size: 54 1425 50 275 18 516 1437 541
Cuts at: 4324 5213 6842 8
Size: 889 1629 43
Fragments arranged by size:
1629 1437 1425 889 541 516 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4324 1537
Size: 275 2512 4090
Fragments arranged by size:
4090 2512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 2214 2417 4807 1353
Size: 650 211 203 2390 3423
Fragments arranged by size:
3423 2390 650 211 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5464 5838 5984 387
Size: 1064 138 3875 374 146 1280
Fragments arranged by size:
3875 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2135 2181 2282 2293 2337 3165
Size: 393 400 103 46 101 11 44 828
Cuts at: 3165 3315 3408 3498 4940 5537 6324 6592 1239
Size: 150 93 90 1442 597 787 268 1524
Fragments arranged by size:
1524 1442 828 787 597 400 393 268
150 103 101 93 90 46 44 11
EcoO109I rG'GnC_Cy
Cuts at: 2834 2834
Size: 6877
EcoRI G'AATT_C
Cuts at: 2131 4186 4564 2131
Size: 2055 378 4444
Fragments arranged by size:
4444 2055 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4041 4041
Size: 6877
FspI TGC'GCA
Cuts at: 5383 5383
Size: 6877
HaeII r_GCGC'y
Cuts at: 1678 2016 2305 6024 1678
Size: 338 289 3719 2531
Fragments arranged by size:
3719 2531 338 289
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2246 2278 3546 3578 4047
Size: 32 135 32 674 32 1268 32 469
Cuts at: 4047 4079 5518 5550 5592 5624 1373
Size: 32 1439 32 42 32 2626
Fragments arranged by size:
2626 1439 1268 674 469 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4221 4467 669
Size: 988 281 2283 246 3079
Fragments arranged by size:
3079 2283 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6877
HpaI GTT'AAC
Cuts at: 4467 4467
Size: 6877
KpnI G_GTAC'C
Cuts at: 4202 4202
Size: 6877
MfeI C'AATT_G
Cuts at: 3514 4476 3514
Size: 962 5915
MluI A'CGCG_T
Cuts at: 6753 6753
Size: 6877
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5991 6175 1305
Size: 4686 184 2007
Fragments arranged by size:
4686 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5055
Fragments arranged by size:
5055 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3992 4097 4835 5194 5353 518
Size: 648 2826 105 738 359 159 2042
Fragments arranged by size:
2826 2042 738 648 359 159 105
MspA1I CmG'CkG
Cuts at: 1606 3122 4293 4970 6118 6363 1606
Size: 1516 1171 677 1148 245 2120
Fragments arranged by size:
2120 1516 1171 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6325
NdeI CA'TA_TG
Cuts at: 387 4002 387
Size: 3615 3262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6877
NotI GC'GGCC_GC
Cuts at: 4324 4324
Size: 6877
NruI TCG'CGA
Cuts at: 3529 3529
Size: 6877
NspI r_CATG'y
Cuts at: 1132 1229 3354 3930 4235 5780 1132
Size: 97 2125 576 305 1545 2229
Fragments arranged by size:
2229 2125 1545 576 305 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6877
PciI A'CATG_T
Cuts at: 3350 3926 5776 3350
Size: 576 1850 4451
Fragments arranged by size:
4451 1850 576
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3449 1108
Size: 2341 4536
PfoI T'CCnGG_A
Cuts at: 3292 3889 3292
Size: 597 6280
PmeI GTTT'AAAC
Cuts at: 4181 4181
Size: 6877
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4137 4169 4946 4978 6459 6491 4137
Size: 32 777 32 1481 32 4523
Fragments arranged by size:
4523 1481 777 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3835 1613
Size: 2222 4655
PsiI TTA'TAA
Cuts at: 4447 4447
Size: 6877
PstI C_TGCA'G
Cuts at: 830 3679 4229 830
Size: 2849 550 3478
Fragments arranged by size:
3478 2849 550
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6877
PvuII CAG'CTG
Cuts at: 3122 3122
Size: 6877
SalI G'TCGA_C
Cuts at: 1936 4219 1936
Size: 2283 4594
SapI GCTCTTCn'nnn_
Cuts at: 2003 2003
Size: 6877
SbfI CC_TGCA'GG
Cuts at: 4229 4229
Size: 6877
ScaI AGT'ACT
Cuts at: 5125 6837 5125
Size: 1712 5165
SexAI A'CCwGG_T
Cuts at: 3022 3022
Size: 6877
SfcI C'TryA_G
Cuts at: 826 1045 3675 4225 5360 6041 6232 826
Size: 219 2630 550 1135 681 191 1471
Fragments arranged by size:
2630 1471 1135 681 550 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6877
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6877
SmaI CCC'GGG
Cuts at: 1872 4204 1872
Size: 2332 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4981 5882 6144 820
Size: 197 926 3038 901 262 1553
Fragments arranged by size:
3038 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4668 493
Size: 4175 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6877
SphI G_CATG'C
Cuts at: 1132 4235 1132
Size: 3103 3774
SspI AAT'ATT
Cuts at: 5 52 2386 3569 4801 5
Size: 47 2334 1183 1232 2081
Fragments arranged by size:
2334 2081 1232 1183 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6877
StyI C'CwwG_G
Cuts at: 513 1065 3846 4316 513
Size: 552 2781 470 3074
Fragments arranged by size:
3074 2781 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3296 4904 4921 5074 5259 5704 325
Size: 1255 1716 1608 17 153 185 445 1498
Fragments arranged by size:
1716 1608 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2657 5123 6835
Size: 274 80 33 51 1443 680 2466 1712
Cuts at: 6835 96
Size: 138
Fragments arranged by size:
2466 1712 1443 680 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6837 6869 6837
Size: 32 6845
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6877
XbaI T'CTAG_A
Cuts at: 4213 4213
Size: 6877
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6877
XmaI C'CCGG_G
Cuts at: 1870 4202 1870
Size: 2332 4545
XmnI GAAnn'nnTTC
Cuts at: 3052 5006 3052
Size: 1954 4923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4685 276
Size: 53 83 186 936 3151 2468
Fragments arranged by size:
3151 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI