Restriction map for
pFN21AE0368
(Circular) (Six-base) MAPSORT of: pFN21AE0368.seq Check: 6510 from: 1 to: 6670
pFN21AE0368 6670 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2407 2565 4013 1937
Size: 470 158 1448 4594
Fragments arranged by size:
4594 1448 470 158
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4733 6117 6385 1632
Size: 3101 1384 268 1917
Fragments arranged by size:
3101 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2243 820
Size: 197 1226 5247
Fragments arranged by size:
5247 1226 197
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 5399 5399
Size: 6670
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AlwNI CAG_nnn'CTG
Cuts at: 2487 3247 4413 5985 2487
Size: 760 1166 1572 3172
Fragments arranged by size:
3172 1572 1166 760
ApaLI G'TGCA_C
Cuts at: 4727 5883 4727
Size: 1156 5514
ApoI r'AATT_y
Cuts at: 1493 3461 3531 3979 4205 4357 6466 1493
Size: 1968 70 448 226 152 2109 1697
Fragments arranged by size:
2109 1968 1697 448 226 152 70
AseI AT'TA_AT
Cuts at: 160 1032 3221 5224 160
Size: 872 2189 2003 1606
Fragments arranged by size:
2189 2003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2074 3855 3889 3995 1870
Size: 73 119 12 1781 34 106 4545
Fragments arranged by size:
4545 1781 119 106 73 34 12
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2199 3991 5446 618
Size: 325 217 627 412 1792 1455 1842
Fragments arranged by size:
1842 1792 1455 627 412 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2145 3132 3254 3816 3862 3989 1391
Size: 678 76 987 122 562 46 127 4072
Fragments arranged by size:
4072 987 678 562 127 122 76 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2396 928
Size: 971 497 5202
Fragments arranged by size:
5202 971 497
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 1903
Size: 34 2924 34 3678
Fragments arranged by size:
3678 2924 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3758 4562 5772 3758
Size: 804 1210 4656
Fragments arranged by size:
4656 1210 804
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5281 136
Size: 107 122 71 631 605 3609 1525
Fragments arranged by size:
3609 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6665 6665
Size: 6670
BlpI GC'TnA_GC
Cuts at: 3248 4087 3248
Size: 839 5831
Bme1580I G_kGCm'C
Cuts at: 1790 2420 3125 4731 5887 1790
Size: 630 705 1606 1156 2573
Fragments arranged by size:
2573 1606 1156 705 630
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 5782
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3243 3649 4401
Size: 416 53 83 399 24 1826 406 752
Cuts at: 4401 5359 442
Size: 958 1753
Fragments arranged by size:
1826 1753 958 752 416 406 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2116 2534 3807 3822 5330 1239
Size: 877 418 1273 15 1508 2579
Fragments arranged by size:
2579 1508 1273 877 418 15
Bpu10I CC'TnA_GC
Cuts at: 2068 3345 2068
Size: 1277 5393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3660 4795 5660 5958 3660
Size: 1135 865 298 4372
Fragments arranged by size:
4372 1135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 2883 6664 2883
Size: 3781 2889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4477 4859 5494 275
Size: 53 83 186 936 2944 382 635 1451
Fragments arranged by size:
2944 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2460 5103 5501 5775 5922 6333 2460
Size: 2643 398 274 147 411 2797
Fragments arranged by size:
2797 2643 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2149 2179 3820 3850 1178
Size: 30 165 30 746 30 1641 30 3998
Fragments arranged by size:
3998 1641 746 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2064 3663 1186
Size: 878 1599 4193
Fragments arranged by size:
4193 1599 878
BseYI C'CCAG_C
Cuts at: 2778 2849 3725 5873 6369 2778
Size: 71 876 2148 496 3079
Fragments arranged by size:
3079 2148 876 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2473 1163
Size: 573 737 5360
Fragments arranged by size:
5360 737 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 4120 4881 5909 1540
Size: 275 186 36 2083 761 1028 2301
Fragments arranged by size:
2301 2083 1028 761 275 186 36
BsiHKAI G_wGCw'C
Cuts at: 2656 3132 3816 3862 4731 4816 5887 2656
Size: 476 684 46 869 85 1071 3439
Fragments arranged by size:
3439 1071 869 684 476 85 46
BsmI GAATG_Cn'
Cuts at: 2861 3596 4181 4274 2861
Size: 735 585 93 5257
Fragments arranged by size:
5257 735 585 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2176 2176
Size: 6670
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2145 2420 2656 3125 3132 3254
Size: 399 279 76 275 236 469 7 122
Cuts at: 3254 3816 3862 3989 4731 4816 5887 1391
Size: 562 46 127 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 562 469 399 279 275
236 127 122 85 76 46 7
BspEI T'CCGG_A
Cuts at: 2460 6333 2460
Size: 3873 2797
BspHI T'CATG_A
Cuts at: 4559 6289 4559
Size: 1730 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 3403 3721 3789 4025 844
Size: 1575 984 318 68 236 3489
Fragments arranged by size:
3489 1575 984 318 236 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2100 4557 1394
Size: 429 277 2457 3507
Fragments arranged by size:
3507 2457 429 277
BsrDI GCAATG_nn'
Cuts at: 65 1248 2561 5165 5339 65
Size: 1183 1313 2604 174 1396
Fragments arranged by size:
2604 1396 1313 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 2196 5314 5501 1700
Size: 110 275 111 3118 187 2869
Fragments arranged by size:
3118 2869 275 187 111 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 3042 4730 5742 3042
Size: 1688 1012 3970
Fragments arranged by size:
3970 1688 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2747 794
Size: 1953 4717
BstBI TT'CG_AA
Cuts at: 2968 5474 2968
Size: 2506 4164
BstEII G'GTnAC_C
Cuts at: 1184 3901 1184
Size: 2717 3953
BstXI CCAn_nnnn'nTGG
Cuts at: 3758 3798 3758
Size: 40 6630
BstYI r'GATC_y
Cuts at: 1972 3832 4000 4367 4752 4769 6221 6307 6319
Size: 1860 168 367 385 17 1452 86 12
Cuts at: 6319 6665 1972
Size: 346 1977
Fragments arranged by size:
1977 1860 1452 385 367 346 168 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2538 2784 2538
Size: 246 6424
BtgI C'CryG_G
Cuts at: 513 1065 2004 2569 3426 513
Size: 552 939 565 857 3757
Fragments arranged by size:
3757 939 857 565 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3182 487
Size: 683 56 1956 3975
Fragments arranged by size:
3975 1956 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 3281 4186 4980 5000 2601
Size: 680 905 794 20 4271
Fragments arranged by size:
4271 905 794 680 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2238 2273 529
Size: 35 1674 35 4926
Fragments arranged by size:
4926 1674 35 35
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 2517 3022 2517
Size: 505 6165
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2222 5677 809
Size: 507 906 3455 1802
Fragments arranged by size:
3455 1802 906 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2658 4117
Size: 54 1425 50 275 18 204 624 1459
Cuts at: 4117 5006 6635 8
Size: 889 1629 43
Fragments arranged by size:
1629 1459 1425 889 624 275 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2034 4117 1537
Size: 275 222 2083 4090
Fragments arranged by size:
4090 2083 275 222
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 4600 1353
Size: 960 2287 3423
Fragments arranged by size:
3423 2287 960
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2061 5257 5631 5777 387
Size: 1064 138 472 3196 374 146 1280
Fragments arranged by size:
3196 1280 1064 472 374 146 138
EcoICRI GAG'CTC
Cuts at: 3130 3814 3860 3130
Size: 684 46 5940
Fragments arranged by size:
5940 684 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2534 3807 3822 4733 5330 6117
Size: 393 484 418 1273 15 911 597 787
Cuts at: 6117 6385 1239
Size: 268 1524
Fragments arranged by size:
1524 1273 911 787 597 484 418 393
268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2462 2462
Size: 6670
EcoO109I rG'GnC_Cy
Cuts at: 3733 3733
Size: 6670
EcoRI G'AATT_C
Cuts at: 3461 3979 4357 3461
Size: 518 378 5774
Fragments arranged by size:
5774 518 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2258 2258
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2095 2157 3552 5817 1678
Size: 417 62 1395 2265 2531
Fragments arranged by size:
2531 2265 1395 417 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2998 3030 3163 3195 3844
Size: 32 135 32 1426 32 133 32 649
Cuts at: 3844 3876 5311 5343 5385 5417 1373
Size: 32 1435 32 42 32 2626
Fragments arranged by size:
2626 1435 1426 649 135 133 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2408 4014 4260 669
Size: 988 281 470 1606 246 3079
Fragments arranged by size:
3079 1606 988 470 281 246
HindIII A'AGCT_T
Cuts at: 748 2357 748
Size: 1609 5061
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5784 5968 1305
Size: 4479 184 2007
Fragments arranged by size:
4479 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2660 10
Size: 54 1425 343 828 4020
Fragments arranged by size:
4020 1425 828 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3477 3712 4628 4987 5146 518
Size: 648 2311 235 916 359 159 2042
Fragments arranged by size:
2311 2042 916 648 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 4086 4763 5911 6156 1606
Size: 2480 677 1148 245 2120
Fragments arranged by size:
2480 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2198 2198
Size: 6670
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5179
Fragments arranged by size:
5179 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NgoMIV G'CCGG_C
Cuts at: 2196 2196
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 4028 5573 1132
Size: 97 2799 1545 2229
Fragments arranged by size:
2799 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2836 1348
Size: 1488 5182
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3733 3733
Size: 6670
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspXI vC'TCGA_Gb
Cuts at: 3889 3889
Size: 6670
PstI C_TGCA'G
Cuts at: 830 3288 3400 4022 830
Size: 2458 112 622 3478
Fragments arranged by size:
3478 2458 622 112
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
SacI G_AGCT'C
Cuts at: 3132 3816 3862 3132
Size: 684 46 5940
Fragments arranged by size:
5940 684 46
SalI G'TCGA_C
Cuts at: 1936 2406 4012 1936
Size: 470 1606 4594
Fragments arranged by size:
4594 1606 470
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 3198 3371 4918 6630 3198
Size: 173 1547 1712 3238
Fragments arranged by size:
3238 1712 1547 173
SfcI C'TryA_G
Cuts at: 826 1045 3284 3396 4018 5153 5834 6025 826
Size: 219 2239 112 622 1135 681 191 1471
Fragments arranged by size:
2239 1471 1135 681 622 219 191 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 2076 3997 1872
Size: 204 1921 4545
Fragments arranged by size:
4545 1921 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 3675 3889 4774 5675 5937
Size: 197 926 300 1432 214 885 901 262
Cuts at: 5937 820
Size: 1553
Fragments arranged by size:
1553 1432 926 901 885 300 262 214
197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SrfI GCCC'GGGC
Cuts at: 2076 2076
Size: 6670
SspI AAT'ATT
Cuts at: 5 52 3513 4594 5
Size: 47 3461 1081 2081
Fragments arranged by size:
3461 2081 1081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 4109 513
Size: 552 939 171 1934 3074
Fragments arranged by size:
3074 1934 939 552 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2588 4697 4714 4867 5052 5497 325
Size: 1255 1008 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3196 3369 4916
Size: 274 80 33 51 1443 1219 173 1547
Cuts at: 4916 6628 96
Size: 1712 138
Fragments arranged by size:
1712 1547 1443 1219 274 173 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2149 2181 6630 6662 2149
Size: 32 4449 32 2157
Fragments arranged by size:
4449 2157 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 3085 4006 3085
Size: 921 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2876 2876
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 3889 1943
Size: 1946 4724
XmaI C'CCGG_G
Cuts at: 1870 2074 3995 1870
Size: 204 1921 4545
Fragments arranged by size:
4545 1921 204
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SwaI