Restriction map for
pFN21AE0366
(Circular) (Six-base) MAPSORT of: pFN21AE0366.seq Check: 7274 from: 1 to: 6607
pFN21AE0366 6607 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2144 2144
Size: 6607
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4417 278
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2967 3522 3950 1937
Size: 1030 555 428 4594
Fragments arranged by size:
4594 1030 555 428
Acc65I G'GTAC_C
Cuts at: 2532 3928 2532
Size: 1396 5211
AclI AA'CG_TT
Cuts at: 1563 4734 5107 1563
Size: 3171 373 3063
Fragments arranged by size:
3171 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2756 2998 3078 3920 4670 6054 6322
Size: 725 399 242 80 842 750 1384 268
Cuts at: 6322 1632
Size: 1917
Fragments arranged by size:
1917 1384 842 750 725 399 268 242
80
AfeI AGC'GCT
Cuts at: 2999 2999
Size: 6607
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6410
AflIII A'CryG_T
Cuts at: 1203 2166 2631 3525 5506 6483 1203
Size: 963 465 894 1981 977 1327
Fragments arranged by size:
1981 1327 977 963 894 465
AgeI A'CCGG_T
Cuts at: 5438 5438
Size: 6607
AhdI GACnn_n'nnGTC
Cuts at: 5336 5336
Size: 6607
AleI CACnn'nnGTG
Cuts at: 1166 2945 1166
Size: 1779 4828
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 1184
Size: 1089 5518
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 6575
AlwNI CAG_nnn'CTG
Cuts at: 2290 3676 3690 3859 4350 5922 2290
Size: 1386 14 169 491 1572 2975
Fragments arranged by size:
2975 1572 1386 491 169 14
ApaLI G'TGCA_C
Cuts at: 2635 2888 4664 5820 2635
Size: 253 1776 1156 3422
Fragments arranged by size:
3422 1776 1156 253
ApoI r'AATT_y
Cuts at: 1493 3710 3916 4142 4294 6403 1493
Size: 2217 206 226 152 2109 1697
Fragments arranged by size:
2217 2109 1697 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 5161 160
Size: 872 4129 1606
Fragments arranged by size:
4129 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
AvaI C'yCGr_G
Cuts at: 1870 1943 2572 3932 1870
Size: 73 629 1360 4545
Fragments arranged by size:
4545 1360 629 73
AvrII C'CTAG_G
Cuts at: 2344 2344
Size: 6607
BamHI G'GATC_C
Cuts at: 2046 3937 4304 2046
Size: 1891 367 4349
Fragments arranged by size:
4349 1891 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2532 2741 3928 5383 618
Size: 325 217 627 745 209 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 745 627 325 217 209
BanII G_rGCy'C
Cuts at: 1391 2321 2513 2579 3926 1391
Size: 930 192 66 1347 4072
Fragments arranged by size:
4072 1347 930 192 66
BbeI G_GCGC'C
Cuts at: 2745 2745
Size: 6607
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 2821 3202 928
Size: 971 853 69 381 4333
Fragments arranged by size:
4333 971 853 381 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4798 4832 1903
Size: 34 2861 34 3678
Fragments arranged by size:
3678 2861 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4499 5709 4499
Size: 1210 5397
BclI T'GATC_A
Cuts at: 1548 3633 1548
Size: 2085 4522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5218 136
Size: 107 122 71 631 605 3546 1525
Fragments arranged by size:
3546 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3729 6602 3729
Size: 2873 3734
BlpI GC'TnA_GC
Cuts at: 2061 4024 2061
Size: 1963 4644
Bme1580I G_kGCm'C
Cuts at: 1790 2639 2892 3191 3330 3783 4668 5824 1790
Size: 849 253 299 139 453 885 1156 2573
Fragments arranged by size:
2573 1156 885 849 453 299 253 139
BmgBI CAC'GTC
Cuts at: 1318 2367 2559 1318
Size: 1049 192 5366
Fragments arranged by size:
5366 1049 192
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3754 4338 5296
Size: 416 53 83 399 24 2337 584 958
Cuts at: 5296 442
Size: 1753
Fragments arranged by size:
2337 1753 958 584 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2561 2926 3529 2110
Size: 451 365 603 5188
Fragments arranged by size:
5188 603 451 365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2264 2288 2473 2857 3795 5267 1239
Size: 848 177 24 185 384 938 1472 2579
Fragments arranged by size:
2579 1472 938 848 384 185 177 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4732 5597 5895 4732
Size: 865 298 5444
Fragments arranged by size:
5444 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 2648 5270 882
Size: 617 564 585 2622 2219
Fragments arranged by size:
2622 2219 617 585 564
BsaAI yAC'GTr
Cuts at: 493 1204 3526 3603 4398 493
Size: 711 2322 77 795 2702
Fragments arranged by size:
2702 2322 795 711 77
BsaBI GATnn'nnATC
Cuts at: 6601 6601
Size: 6607
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2742 3318 4414
Size: 53 83 186 936 507 702 576 1096
Cuts at: 4414 4796 5431 275
Size: 382 635 1451
Fragments arranged by size:
1451 1096 936 702 635 576 507 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5040 5438 5712 5859 6270 5040
Size: 398 274 147 411 5377
Fragments arranged by size:
5377 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 2666 2696 3434
Size: 30 165 30 1158 30 75 30 738
Cuts at: 3434 3464 1178
Size: 30 4321
Fragments arranged by size:
4321 1158 738 165 75 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2217 2220 1186
Size: 913 118 3 5573
Fragments arranged by size:
5573 913 118 3
BseYI C'CCAG_C
Cuts at: 2793 5810 6306 2793
Size: 3017 496 3094
Fragments arranged by size:
3094 3017 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6034
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 4057 4818 5846 1540
Size: 275 186 101 1955 761 1028 2301
Fragments arranged by size:
2301 1955 1028 761 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2321 2513 2639 2892 3261 3378 3869 4668 4753
Size: 192 126 253 369 117 491 799 85
Cuts at: 4753 5824 2321
Size: 1071 3104
Fragments arranged by size:
3104 1071 799 491 369 253 192 126
117 85
BsmI GAATG_Cn'
Cuts at: 4118 4211 4118
Size: 93 6514
BsmBI CGTCTCn'nnnn_
Cuts at: 2534 2534
Size: 6607
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2321 2513 2579 2639 2892 3191 3261
Size: 399 531 192 66 60 253 299 70
Cuts at: 3261 3330 3378 3783 3869 3926 4668 4753 5824
Size: 69 48 405 86 57 742 85 1071
Cuts at: 5824 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 531 405 399 299 253
192 86 85 70 69 66 60 57
48
BspEI T'CCGG_A
Cuts at: 6270 6270
Size: 6607
BspHI T'CATG_A
Cuts at: 2214 3564 4496 6226 2214
Size: 1350 932 1730 2595
Fragments arranged by size:
2595 1730 1350 932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2144 3060 3158 3962 844
Size: 1300 916 98 804 3489
Fragments arranged by size:
3489 1300 916 804 98
BsrBI CCG'CTC
Cuts at: 1394 1823 2467 4494 1394
Size: 429 644 2027 3507
Fragments arranged by size:
3507 2027 644 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5102 5276 65
Size: 1183 3854 174 1396
Fragments arranged by size:
3854 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3470 5251 5438 1700
Size: 110 1660 1781 187 2869
Fragments arranged by size:
2869 1781 1660 187 110
BsrGI T'GTAC_A
Cuts at: 96 2628 2673 96
Size: 2532 45 4030
Fragments arranged by size:
4030 2532 45
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6607
BssSI C'ACGA_G
Cuts at: 2015 4667 5679 2015
Size: 2652 1012 2943
Fragments arranged by size:
2943 2652 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2128 2290 794
Size: 1334 162 5111
Fragments arranged by size:
5111 1334 162
BstBI TT'CG_AA
Cuts at: 5411 5411
Size: 6607
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6607
BstYI r'GATC_y
Cuts at: 1972 2046 3729 3937 4304 4689 4706 6158 6244
Size: 74 1683 208 367 385 17 1452 86
Cuts at: 6244 6256 6602 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1683 1452 385 367 346 208 86
74 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 2644 3120 3180 513
Size: 552 939 193 447 476 60 3940
Fragments arranged by size:
3940 939 552 476 447 193 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3478 487
Size: 683 56 2252 3616
Fragments arranged by size:
3616 2252 683 56
BtsI GCAGTG_nn'
Cuts at: 3829 4123 4917 4937 3829
Size: 294 794 20 5499
Fragments arranged by size:
5499 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6607
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3584 3619 529
Size: 35 3020 35 3517
Fragments arranged by size:
3517 3020 35 35
DraI TTT'AAA
Cuts at: 3911 3911
Size: 6607
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3344 5614 809
Size: 507 2028 2270 1802
Fragments arranged by size:
2270 2028 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 2955 3436
Size: 54 1425 50 275 18 269 856 481
Cuts at: 3436 3571 4054 4943 6572 8
Size: 135 483 889 1629 43
Fragments arranged by size:
1629 1425 889 856 483 481 275 269
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2099 4054 1537
Size: 275 287 1955 4090
Fragments arranged by size:
4090 1955 287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2771 3266 3281 3650 4537 1353
Size: 1418 495 15 369 887 3423
Fragments arranged by size:
3423 1418 887 495 369 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5194 5568 5714 387
Size: 1064 138 3605 374 146 1280
Fragments arranged by size:
3605 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2319 2511 2319
Size: 192 6415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2264 2288 2357 2473 2756 2857
Size: 393 455 177 24 69 116 283 101
Cuts at: 2857 2998 3078 3795 3920 4670 5267 6054 6322
Size: 141 80 717 125 750 597 787 268
Cuts at: 6322 1239
Size: 1524
Fragments arranged by size:
1524 787 750 717 597 455 393 283
268 177 141 125 116 101 80 69
24
EcoNI CCTnn'n_nnAGG
Cuts at: 3173 3173
Size: 6607
EcoO109I rG'GnC_Cy
Cuts at: 2341 2857 3337 3694 3895 2341
Size: 516 480 357 201 5053
Fragments arranged by size:
5053 516 480 357 201
EcoRI G'AATT_C
Cuts at: 3916 4294 3916
Size: 378 6229
FspI TGC'GCA
Cuts at: 2614 5113 2614
Size: 2499 4108
HaeII r_GCGC'y
Cuts at: 1678 2176 2745 3001 5754 1678
Size: 498 569 256 2753 2531
Fragments arranged by size:
2753 2531 569 498 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2561 2593 5248 5280 5322
Size: 32 135 32 989 32 2655 32 42
Cuts at: 5322 5354 1373
Size: 32 2626
Fragments arranged by size:
2655 2626 989 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3951 4197 669
Size: 988 281 2013 246 3079
Fragments arranged by size:
3079 2013 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6607
HpaI GTT'AAC
Cuts at: 4197 4197
Size: 6607
KasI G'GCGC_C
Cuts at: 2741 2741
Size: 6607
KpnI G_GTAC'C
Cuts at: 2536 3932 2536
Size: 1396 5211
MfeI C'AATT_G
Cuts at: 4206 4206
Size: 6607
MluI A'CGCG_T
Cuts at: 6483 6483
Size: 6607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3138 5721 5905 1305
Size: 1833 2583 184 2007
Fragments arranged by size:
2583 2007 1833 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2957 10
Size: 54 1425 343 1125 3660
Fragments arranged by size:
3660 1425 1125 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 3230 4565 4924 5083 518
Size: 648 1779 285 1335 359 159 2042
Fragments arranged by size:
2042 1779 1335 648 359 285 159
MspA1I CmG'CkG
Cuts at: 1606 2287 2646 3100 3122 3136 4023 4700 5848
Size: 681 359 454 22 14 887 677 1148
Cuts at: 5848 6093 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 887 681 677 454 359 245
22 14
NaeI GCC'GGC
Cuts at: 3472 3472
Size: 6607
NarI GG'CG_CC
Cuts at: 2742 2742
Size: 6607
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5116
Fragments arranged by size:
5116 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6607
NgoMIV G'CCGG_C
Cuts at: 3470 3470
Size: 6607
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6607
NotI GC'GGCC_GC
Cuts at: 4054 4054
Size: 6607
NspI r_CATG'y
Cuts at: 1132 1229 2131 2635 3094 3965 5510 1132
Size: 97 902 504 459 871 1545 2229
Fragments arranged by size:
2229 1545 902 871 504 459 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6607
PciI A'CATG_T
Cuts at: 2631 5506 2631
Size: 2875 3732
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2586 1108
Size: 1478 5129
PfoI T'CCnGG_A
Cuts at: 2241 2405 2436 2241
Size: 164 31 6412
Fragments arranged by size:
6412 164 31
PmeI GTTT'AAAC
Cuts at: 3911 3911
Size: 6607
PmlI CAC'GTG
Cuts at: 3603 3603
Size: 6607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 6189 6221 4676
Size: 32 1481 32 5062
Fragments arranged by size:
5062 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3337 3694 3895 3337
Size: 357 201 6049
Fragments arranged by size:
6049 357 201
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6607
PsiI TTA'TAA
Cuts at: 4177 4177
Size: 6607
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2255 2287 2255
Size: 32 6575
PstI C_TGCA'G
Cuts at: 830 3336 3959 830
Size: 2506 623 3478
Fragments arranged by size:
3478 2506 623
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6607
PvuII CAG'CTG
Cuts at: 3136 3136
Size: 6607
SacI G_AGCT'C
Cuts at: 2321 2513 2321
Size: 192 6415
SacII CC_GC'GG
Cuts at: 2647 3123 2647
Size: 476 6131
SalI G'TCGA_C
Cuts at: 1936 3949 1936
Size: 2013 4594
SanDI GG'GwC_CC
Cuts at: 3694 3694
Size: 6607
SapI GCTCTTCn'nnn_
Cuts at: 3266 3281 3266
Size: 15 6592
SbfI CC_TGCA'GG
Cuts at: 3336 3959 3336
Size: 623 5984
ScaI AGT'ACT
Cuts at: 3443 4855 6567 3443
Size: 1412 1712 3483
Fragments arranged by size:
3483 1712 1412
SfcI C'TryA_G
Cuts at: 826 1045 3332 3682 3955 5090 5771 5962 826
Size: 219 2287 350 273 1135 681 191 1471
Fragments arranged by size:
2287 1471 1135 681 350 273 219 191
SfoI GGC'GCC
Cuts at: 2743 2743
Size: 6607
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6607
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6607
SmaI CCC'GGG
Cuts at: 1872 3934 1872
Size: 2062 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4711 5612 5874 820
Size: 197 926 2768 901 262 1553
Fragments arranged by size:
2768 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4398 493
Size: 3905 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6607
SphI G_CATG'C
Cuts at: 1132 3965 1132
Size: 2833 3774
SspI AAT'ATT
Cuts at: 5 52 4531 5
Size: 47 4479 2081
Fragments arranged by size:
4479 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 2727 3195 3384 1517
Size: 832 378 468 189 4740
Fragments arranged by size:
4740 832 468 378 189
StyI C'CwwG_G
Cuts at: 513 1065 2004 2344 3366 4046 513
Size: 552 939 340 1022 680 3074
Fragments arranged by size:
3074 1022 939 680 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 3736 4634 4651 4804 4989 5434
Size: 1255 717 1439 898 17 153 185 445
Cuts at: 5434 325
Size: 1498
Fragments arranged by size:
1498 1439 1255 898 717 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2124 2628 2673
Size: 274 80 33 51 1443 147 504 45
Cuts at: 2673 3441 4853 6565 96
Size: 768 1412 1712 138
Fragments arranged by size:
1712 1443 1412 768 504 274 147 138
80 51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6567 6599 6567
Size: 32 6575
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6607
XbaI T'CTAG_A
Cuts at: 3943 3943
Size: 6607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6607
XmaI C'CCGG_G
Cuts at: 1870 3932 1870
Size: 2062 4545
XmnI GAAnn'nnTTC
Cuts at: 4736 4736
Size: 6607
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4415 276
Size: 53 83 186 936 2881 2468
Fragments arranged by size:
2881 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI BaeI BbvCI BfrBI Bpu10I BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI RsrII SexAI SfiI SrfI
SwaI XcmI