Restriction map for
pFN21AE0365
(Circular) (Six-base) MAPSORT of: pFN21AE0365.seq Check: 3654 from: 1 to: 6265
pFN21AE0365 6265 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 2504 2611 3110 3179 4328 5712 5980
Size: 776 96 107 499 69 1149 1384 268
Cuts at: 5980 1632
Size: 1917
Fragments arranged by size:
1917 1384 1149 776 499 268 107 96
69
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2807 1184
Size: 1623 4642
AlwNI CAG_nnn'CTG
Cuts at: 2565 2781 2969 3314 3405 4008 5580 2565
Size: 216 188 345 91 603 1572 3250
Fragments arranged by size:
3250 1572 603 345 216 188 91
ApaI G_GGCC'C
Cuts at: 2285 3399 2285
Size: 1114 5151
ApaLI G'TGCA_C
Cuts at: 2792 4322 5478 2792
Size: 1530 1156 3579
Fragments arranged by size:
3579 1530 1156
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2826 3042 3586 5041 618
Size: 325 217 627 1039 216 544 1455 1842
Fragments arranged by size:
1842 1455 1039 627 544 325 217 216
BanII G_rGCy'C
Cuts at: 1391 2285 2412 2688 3399 3584 1391
Size: 894 127 276 711 185 4072
Fragments arranged by size:
4072 894 711 276 185 127
BbeI G_GCGC'C
Cuts at: 2830 2830
Size: 6265
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2365 928
Size: 971 466 4828
Fragments arranged by size:
4828 971 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BfrBI ATG'CAT
Cuts at: 2527 2622 2527
Size: 95 6170
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4876 136
Size: 107 122 71 631 605 3204 1525
Fragments arranged by size:
3204 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6260 6260
Size: 6265
BlpI GC'TnA_GC
Cuts at: 2015 2915 3682 2015
Size: 900 767 4598
Fragments arranged by size:
4598 900 767
Bme1580I G_kGCm'C
Cuts at: 1790 2285 2695 2796 3399 4326 5482 1790
Size: 495 410 101 603 927 1156 2573
Fragments arranged by size:
2573 1156 927 603 495 410 101
BmgBI CAC'GTC
Cuts at: 1318 3220 1318
Size: 1902 4363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3127 3996
Size: 416 53 83 399 24 1235 475 869
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1235 958 869 475 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4925 1239
Size: 3686 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2245 4390 5255 5553 2245
Size: 2145 865 298 2957
Fragments arranged by size:
2957 2145 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2459 2827 3506 4072
Size: 53 83 186 936 926 368 679 566
Cuts at: 4072 4454 5089 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 926 679 635 566 382 368
186 83 53
BsaWI w'CCGG_w
Cuts at: 2148 2724 4698 5096 5370 5517 5928 2148
Size: 576 1974 398 274 147 411 2485
Fragments arranged by size:
2485 1974 576 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6040
Fragments arranged by size:
6040 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2231 1186
Size: 1045 5220
BseYI C'CCAG_C
Cuts at: 2029 2238 2398 5468 5964 2029
Size: 209 160 3070 496 2330
Fragments arranged by size:
3070 2330 496 209 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3193 1163
Size: 573 1457 4235
Fragments arranged by size:
4235 1457 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2140 3715 4476 5504 1540
Size: 275 186 139 1575 761 1028 2301
Fragments arranged by size:
2301 1575 1028 761 275 186 139
BsiHKAI G_wGCw'C
Cuts at: 2586 2688 2796 2910 3228 4326 4411 5482 2586
Size: 102 108 114 318 1098 85 1071 3369
Fragments arranged by size:
3369 1098 1071 318 114 108 102 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2412 2586 2688 2695 2796 2910
Size: 399 495 127 174 102 7 101 114
Cuts at: 2910 3228 3399 3584 4326 4411 5482 1391
Size: 318 171 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 495 399 318 185 174
171 127 114 102 101 85 7
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2824 4152 1394
Size: 429 1001 1328 3507
Fragments arranged by size:
3507 1328 1001 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 3066 4909 5096 1700
Size: 110 231 1025 1843 187 2869
Fragments arranged by size:
2869 1843 1025 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 3027 96
Size: 2931 3334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3076 794
Size: 2282 3983
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 2189 3363 1184
Size: 1005 1174 4086
Fragments arranged by size:
4086 1174 1005
BstXI CCAn_nnnn'nTGG
Cuts at: 2890 2890
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 3595 3962 4347 4364 5816 5902 5914 6260
Size: 1623 367 385 17 1452 86 12 346
Cuts at: 6260 1972
Size: 1977
Fragments arranged by size:
1977 1623 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2036 3315 2036
Size: 1279 4986
BtgI C'CryG_G
Cuts at: 513 1065 2057 2550 3000 3202 513
Size: 552 992 493 450 202 3576
Fragments arranged by size:
3576 992 552 493 450 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3440 487
Size: 683 56 2214 3312
Fragments arranged by size:
3312 2214 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2445 3239 3317 5272 809
Size: 507 1129 794 78 1955 1802
Fragments arranged by size:
1955 1802 1129 794 507 78
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2247 2547
Size: 54 1425 50 275 18 307 110 300
Cuts at: 2547 3003 3305 3712 4601 6230 8
Size: 456 302 407 889 1629 43
Fragments arranged by size:
1629 1425 889 456 407 307 302 300
275 110 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2137 3712 1537
Size: 275 325 1575 4090
Fragments arranged by size:
4090 1575 325 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3353 4195 1353
Size: 2000 842 3423
Fragments arranged by size:
3423 2000 842
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2686 2686
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2504 2611 3110 3179 4328 4925
Size: 393 776 96 107 499 69 1149 597
Cuts at: 4925 5712 5980 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1149 787 776 597 499 393 268
107 96 69
EcoO109I rG'GnC_Cy
Cuts at: 2082 2230 2282 2778 3395 2082
Size: 148 52 496 617 4952
Fragments arranged by size:
4952 617 496 148 52
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2770 2830 3455 5412 1678
Size: 1092 60 625 1957 2531
Fragments arranged by size:
2531 1957 1092 625 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2319 2351 4906 4938 4980
Size: 32 135 32 747 32 2555 32 42
Cuts at: 4980 5012 1373
Size: 32 2626
Fragments arranged by size:
2626 2555 747 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KasI G'GCGC_C
Cuts at: 2826 2826
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 2641 3864 2641
Size: 1223 5042
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2249 2549 3307 10
Size: 54 1425 343 417 300 758 2968
Fragments arranged by size:
2968 1425 758 417 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2056 2978 4223 4582 4741 518
Size: 648 890 922 1245 359 159 2042
Fragments arranged by size:
2042 1245 922 890 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 3204 3681 4358 5506 5751 1606
Size: 636 962 477 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 677 636 477 245
NaeI GCC'GGC
Cuts at: 3068 3068
Size: 6265
NarI GG'CG_CC
Cuts at: 2827 2827
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2550 513
Size: 552 1485 4228
Fragments arranged by size:
4228 1485 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 3066 3066
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NruI TCG'CGA
Cuts at: 3038 3425 3038
Size: 387 5878
NsiI A_TGCA'T
Cuts at: 2529 2624 2529
Size: 95 6170
NspI r_CATG'y
Cuts at: 1132 1229 2531 3623 5168 1132
Size: 97 1302 1092 1545 2229
Fragments arranged by size:
2229 1545 1302 1092 97
PasI CC'CwG_GG
Cuts at: 1348 2260 3559 1348
Size: 912 1299 4054
Fragments arranged by size:
4054 1299 912
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2245 3220 1108
Size: 1137 975 4153
Fragments arranged by size:
4153 1137 975
PfoI T'CCnGG_A
Cuts at: 2817 2817
Size: 6265
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2230
Size: 6265
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2281 3395 2281
Size: 1114 5151
PstI C_TGCA'G
Cuts at: 830 2616 2786 3617 830
Size: 1786 170 831 3478
Fragments arranged by size:
3478 1786 831 170
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2242 2242
Size: 6265
SacI G_AGCT'C
Cuts at: 2688 2688
Size: 6265
SacII CC_GC'GG
Cuts at: 3205 3205
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2612 2782 3613 4748 5429 5620 826
Size: 219 1567 170 831 1135 681 191 1471
Fragments arranged by size:
1567 1471 1135 831 681 219 191 170
SfoI GGC'GCC
Cuts at: 2828 2828
Size: 6265
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 4369 5270 5532 820
Size: 197 926 281 2145 901 262 1553
Fragments arranged by size:
2145 1553 926 901 281 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 2531 3623 1132
Size: 1399 1092 3774
Fragments arranged by size:
3774 1399 1092
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2550 2871 3704 513
Size: 552 1485 321 833 3074
Fragments arranged by size:
3074 1485 833 552 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3060 3227 4292 4309 4462 4647 5092
Size: 1255 1480 167 1065 17 153 185 445
Cuts at: 5092 325
Size: 1498
Fragments arranged by size:
1498 1480 1255 1065 445 185 167 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 3027 4511
Size: 274 80 33 51 1443 765 285 1484
Cuts at: 4511 6223 96
Size: 1712 138
Fragments arranged by size:
1712 1484 1443 765 285 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 2321 2369 1316
Size: 1005 48 5212
Fragments arranged by size:
5212 1005 48
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 2180 4394 2180
Size: 2214 4051
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI PacI PmlI PspXI PsrI RsrII SanDI
SapI SexAI SfiI SrfI SwaI XcmI