Restriction map for
pFN21AE0363
(Circular) (Six-base) MAPSORT of: pFN21AE0363.seq Check: 46 from: 1 to: 6940
pFN21AE0363 6940 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4750 278
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 4283 1937
Size: 2082 264 4594
Fragments arranged by size:
4594 2082 264
Acc65I G'GTAC_C
Cuts at: 4261 4261
Size: 6940
AclI AA'CG_TT
Cuts at: 1563 5067 5440 1563
Size: 3504 373 3063
Fragments arranged by size:
3504 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 2621 2751 2919 3381 3915 5003 6387
Size: 825 164 130 168 462 534 1088 1384
Cuts at: 6387 6655 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1088 825 534 462 268 168
164 130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 4105 5839 6816 1203
Size: 2902 1734 977 1327
Fragments arranged by size:
2902 1734 1327 977
AgeI A'CCGG_T
Cuts at: 2285 5771 2285
Size: 3486 3454
AhdI GACnn_n'nnGTC
Cuts at: 5669 5669
Size: 6940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 5799
AlwNI CAG_nnn'CTG
Cuts at: 2213 4683 6255 2213
Size: 2470 1572 2898
Fragments arranged by size:
2898 2470 1572
ApaLI G'TGCA_C
Cuts at: 4997 6153 4997
Size: 1156 5784
ApoI r'AATT_y
Cuts at: 1493 2531 3092 3356 3926 4249 4475 4627 6736
Size: 1038 561 264 570 323 226 152 2109
Cuts at: 6736 1493
Size: 1697
Fragments arranged by size:
2109 1697 1038 570 561 323 264 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5494 160
Size: 872 4462 1606
Fragments arranged by size:
4462 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 4265 1870
Size: 73 2322 4545
Fragments arranged by size:
4545 2322 73
BamHI G'GATC_C
Cuts at: 4270 4637 4270
Size: 367 6573
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4261 5716 618
Size: 325 217 627 2474 1455 1842
Fragments arranged by size:
2474 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2034 4259 1391
Size: 643 2225 4072
Fragments arranged by size:
4072 2225 643
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2288 2903 928
Size: 971 389 615 4965
Fragments arranged by size:
4965 971 615 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5131 5165 1903
Size: 34 50 34 3110 34 3678
Fragments arranged by size:
3678 3110 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4832 6042 4832
Size: 1210 5730
BclI T'GATC_A
Cuts at: 1548 4078 1548
Size: 2530 4410
BfrBI ATG'CAT
Cuts at: 3333 3333
Size: 6940
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5551 136
Size: 107 122 71 631 605 3879 1525
Fragments arranged by size:
3879 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6935 6935
Size: 6940
BlpI GC'TnA_GC
Cuts at: 4195 4357 4195
Size: 162 6778
Bme1580I G_kGCm'C
Cuts at: 1790 5001 6157 1790
Size: 3211 1156 2573
Fragments arranged by size:
3211 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3016 3958 4671
Size: 416 53 83 399 24 1599 942 713
Cuts at: 4671 5629 442
Size: 958 1753
Fragments arranged by size:
1753 1599 958 942 713 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3841 5600 1239
Size: 2602 1759 2579
Fragments arranged by size:
2602 2579 1759
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 5065 5930 6228 2216
Size: 2849 865 298 2928
Fragments arranged by size:
2928 2849 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 2837 3384 4056 5603 882
Size: 617 1090 248 547 672 1547 2219
Fragments arranged by size:
2219 1547 1090 672 617 547 248
BsaAI yAC'GTr
Cuts at: 493 1204 4731 493
Size: 711 3527 2702
Fragments arranged by size:
3527 2702 711
BsaBI GATnn'nnATC
Cuts at: 2953 6934 2953
Size: 3981 2959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4747 5129 5764 275
Size: 53 83 186 936 3214 382 635 1451
Fragments arranged by size:
3214 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2285 5373 5771 6045 6192 6603 2285
Size: 3088 398 274 147 411 2622
Fragments arranged by size:
3088 2622 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2466 2496 4110 4140 1178
Size: 30 165 30 1063 30 1614 30 3978
Fragments arranged by size:
3978 1614 1063 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2010 2490 3525 3738 1186
Size: 824 480 1035 213 4388
Fragments arranged by size:
4388 1035 824 480 213
BseYI C'CCAG_C
Cuts at: 2297 4200 6143 6639 2297
Size: 1903 1943 496 2598
Fragments arranged by size:
2598 1943 1903 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2199 2555 3246 3696 1163
Size: 573 463 356 691 450 4407
Fragments arranged by size:
4407 691 573 463 450 356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4390 5151 6179 1540
Size: 275 186 2389 761 1028 2301
Fragments arranged by size:
2389 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5001 5086 6157 5001
Size: 85 1071 5784
Fragments arranged by size:
5784 1071 85
BsmI GAATG_Cn'
Cuts at: 2883 4451 4544 2883
Size: 1568 93 5279
Fragments arranged by size:
5279 1568 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 4259 5001 5086 6157 1391
Size: 399 244 2225 742 85 1071 2174
Fragments arranged by size:
2225 2174 1071 742 399 244 85
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 2143 4829 6559 2143
Size: 2686 1730 2524
Fragments arranged by size:
2686 2524 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3058 4295 844
Size: 2214 1237 3489
Fragments arranged by size:
3489 2214 1237
BsrBI CCG'CTC
Cuts at: 1394 1823 4827 1394
Size: 429 3004 3507
Fragments arranged by size:
3507 3004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2319 3925 4128 5435 5609 65
Size: 1183 1071 1606 203 1307 174 1396
Fragments arranged by size:
1606 1396 1307 1183 1071 203 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2285 5584 5771 1700
Size: 110 475 3299 187 2869
Fragments arranged by size:
3299 2869 475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6940
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 5000 6012 5000
Size: 1012 5928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2213 3330 4127 794
Size: 1419 1117 797 3607
Fragments arranged by size:
3607 1419 1117 797
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6940
BstYI r'GATC_y
Cuts at: 1972 2209 4270 4637 5022 5039 6491 6577 6589
Size: 237 2061 367 385 17 1452 86 12
Cuts at: 6589 6935 1972
Size: 346 1977
Fragments arranged by size:
2061 1977 1452 385 367 346 237 86
17 12
BstZ17I GTA'TAC
Cuts at: 4020 4020
Size: 6940
Bsu36I CC'TnA_GG
Cuts at: 2022 3777 2022
Size: 1755 5185
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3343 487
Size: 683 56 2117 4084
Fragments arranged by size:
4084 2117 683 56
BtsI GCAGTG_nn'
Cuts at: 3369 3756 4456 5250 5270 3369
Size: 387 700 794 20 5039
Fragments arranged by size:
5039 794 700 387 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6905
DraI TTT'AAA
Cuts at: 2340 4244 2340
Size: 1904 5036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5947 809
Size: 507 4631 1802
Fragments arranged by size:
4631 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4387 5276 6905
Size: 54 1425 50 275 18 2557 889 1629
Cuts at: 6905 8
Size: 43
Fragments arranged by size:
2557 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4387 1537
Size: 275 2575 4090
Fragments arranged by size:
4090 2575 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 3161 3396 3891 4870 1353
Size: 762 1046 235 495 979 3423
Fragments arranged by size:
3423 1046 979 762 495 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2457 2621 2751 2919 3381 3841 3915
Size: 393 825 164 130 168 462 460 74
Cuts at: 3915 5003 5600 6387 6655 1239
Size: 1088 597 787 268 1524
Fragments arranged by size:
1524 1088 825 787 597 462 460 393
268 168 164 130 74
EcoRI G'AATT_C
Cuts at: 4249 4627 4249
Size: 378 6562
EcoRV GAT'ATC
Cuts at: 3817 3817
Size: 6940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2888 3478 2888
Size: 590 6350
FspI TGC'GCA
Cuts at: 5446 5446
Size: 6940
HaeII r_GCGC'y
Cuts at: 1678 6087 1678
Size: 4409 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 5581 5613 5655
Size: 32 135 32 1187 32 2790 32 42
Cuts at: 5655 5687 1373
Size: 32 2626
Fragments arranged by size:
2790 2626 1187 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3598 4284 4530 669
Size: 988 281 1660 686 246 3079
Fragments arranged by size:
3079 1660 988 686 281 246
HindIII A'AGCT_T
Cuts at: 748 4192 748
Size: 3444 3496
HpaI GTT'AAC
Cuts at: 4530 4530
Size: 6940
KpnI G_GTAC'C
Cuts at: 4265 4265
Size: 6940
MfeI C'AATT_G
Cuts at: 3237 4539 3237
Size: 1302 5638
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6054 6238 1305
Size: 4749 184 2007
Fragments arranged by size:
4749 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5118
Fragments arranged by size:
5118 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2852 4898 5257 5416 518
Size: 648 1686 2046 359 159 2042
Fragments arranged by size:
2046 2042 1686 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2079 4015 4356 5033 6181 6426 1606
Size: 455 18 1936 341 677 1148 245 2120
Fragments arranged by size:
2120 1936 1148 677 455 341 245 18
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6388
NdeI CA'TA_TG
Cuts at: 387 2779 3990 3999 387
Size: 2392 1211 9 3328
Fragments arranged by size:
3328 2392 1211 9
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6940
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NsiI A_TGCA'T
Cuts at: 3335 3335
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 3341 4109 4298 5843 1132
Size: 97 2112 768 189 1545 2229
Fragments arranged by size:
2229 2112 1545 768 189 97
PasI CC'CwG_GG
Cuts at: 1348 2034 1348
Size: 686 6254
PciI A'CATG_T
Cuts at: 4105 5839 4105
Size: 1734 5206
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6940
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2340 2372 5009 5041 6522 6554 2340
Size: 32 2637 32 1481 32 2726
Fragments arranged by size:
2726 2637 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 4510 4510
Size: 6940
PstI C_TGCA'G
Cuts at: 830 4292 830
Size: 3462 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SapI GCTCTTCn'nnn_
Cuts at: 3161 3161
Size: 6940
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 5188 6900 5188
Size: 1712 5228
SfcI C'TryA_G
Cuts at: 826 1045 4179 4288 5423 6104 6295 826
Size: 219 3134 109 1135 681 191 1471
Fragments arranged by size:
3134 1471 1135 681 219 191 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6940
SmaI CCC'GGG
Cuts at: 1872 4267 1872
Size: 2395 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 5044 5945 6207 820
Size: 197 926 288 2813 901 262 1553
Fragments arranged by size:
2813 1553 926 901 288 262 197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6940
SphI G_CATG'C
Cuts at: 1132 4298 1132
Size: 3166 3774
SspI AAT'ATT
Cuts at: 5 52 3001 3414 3649 3958 4035 4864 5
Size: 47 2949 413 235 309 77 829 2081
Fragments arranged by size:
2949 2081 829 413 309 235 77 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6940
StyI C'CwwG_G
Cuts at: 513 1065 4379 513
Size: 552 3314 3074
Fragments arranged by size:
3314 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2055 4967 4984 5137 5322 5767 325
Size: 1255 475 2912 17 153 185 445 1498
Fragments arranged by size:
2912 1498 1255 475 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 5186 6898
Size: 274 80 33 51 1443 471 2738 1712
Cuts at: 6898 96
Size: 138
Fragments arranged by size:
2738 1712 1443 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6900 6932 6900
Size: 32 6908
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 4276 4276
Size: 6940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 4265 1870
Size: 2395 4545
XmnI GAAnn'nnTTC
Cuts at: 5069 5069
Size: 6940
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4748 276
Size: 53 83 186 936 3214 2468
Fragments arranged by size:
3214 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI