Restriction map for
pFN21AE0358
(Circular) (Six-base) MAPSORT of: pFN21AE0358.seq Check: 7452 from: 1 to: 6967
pFN21AE0358 6967 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4777 278
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4310 1937
Size: 2373 4594
Acc65I G'GTAC_C
Cuts at: 4288 4288
Size: 6967
AclI AA'CG_TT
Cuts at: 1563 5094 5467 1563
Size: 3531 373 3063
Fragments arranged by size:
3531 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2386 2583 3048 3332 5030 6414 6682 1632
Size: 754 197 465 284 1698 1384 268 1917
Fragments arranged by size:
1917 1698 1384 754 465 284 268 197
AflII C'TTAA_G
Cuts at: 820 1017 3737 820
Size: 197 2720 4050
Fragments arranged by size:
4050 2720 197
AflIII A'CryG_T
Cuts at: 1203 2378 2899 3302 3997 5866 6843 1203
Size: 1175 521 403 695 1869 977 1327
Fragments arranged by size:
1869 1327 1175 977 695 521 403
AgeI A'CCGG_T
Cuts at: 5798 5798
Size: 6967
AhdI GACnn_n'nnGTC
Cuts at: 5696 5696
Size: 6967
AleI CACnn'nnGTG
Cuts at: 1166 3303 1166
Size: 2137 4830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6967
AlwNI CAG_nnn'CTG
Cuts at: 4710 6282 4710
Size: 1572 5395
ApaLI G'TGCA_C
Cuts at: 3421 5024 6180 3421
Size: 1603 1156 4208
Fragments arranged by size:
4208 1603 1156
ApoI r'AATT_y
Cuts at: 1493 2679 2910 3110 3608 4197 4276 4502 4654
Size: 1186 231 200 498 589 79 226 152
Cuts at: 4654 6763 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1186 589 498 231 226 200
152 79
AseI AT'TA_AT
Cuts at: 160 1032 5521 160
Size: 872 4489 1606
Fragments arranged by size:
4489 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 4292 1870
Size: 73 69 2280 4545
Fragments arranged by size:
4545 2280 73 69
AvrII C'CTAG_G
Cuts at: 2245 2245
Size: 6967
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4141 4174 4141
Size: 33 6934
BamHI G'GATC_C
Cuts at: 2336 3742 4297 4664 2336
Size: 1406 555 367 4639
Fragments arranged by size:
4639 1406 555 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2488 2498 3970 4288 5743
Size: 325 217 627 701 10 1472 318 1455
Cuts at: 5743 618
Size: 1842
Fragments arranged by size:
1842 1472 1455 701 627 325 318 217
10
BanII G_rGCy'C
Cuts at: 1391 4115 4286 1391
Size: 2724 171 4072
Fragments arranged by size:
4072 2724 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 3726 928
Size: 971 675 1152 4169
Fragments arranged by size:
4169 1152 971 675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5158 5192 1903
Size: 34 50 34 3137 34 3678
Fragments arranged by size:
3678 3137 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3608 4142 4859 6069 3608
Size: 534 717 1210 4506
Fragments arranged by size:
4506 1210 717 534
BclI T'GATC_A
Cuts at: 1548 2860 1548
Size: 1312 5655
BfrBI ATG'CAT
Cuts at: 3171 3171
Size: 6967
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5578 136
Size: 107 122 71 631 605 3906 1525
Fragments arranged by size:
3906 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3678 6962 3678
Size: 3284 3683
BlpI GC'TnA_GC
Cuts at: 4384 4384
Size: 6967
Bme1580I G_kGCm'C
Cuts at: 1790 3425 5028 6184 1790
Size: 1635 1603 1156 2573
Fragments arranged by size:
2573 1635 1603 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6967
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3023 4698 5656
Size: 416 53 83 399 24 1606 1675 958
Cuts at: 5656 442
Size: 1753
Fragments arranged by size:
1753 1675 1606 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6967
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 6967
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3711 3929 5627 1239
Size: 2472 218 1698 2579
Fragments arranged by size:
2579 2472 1698 218
Bpu10I CC'TnA_GC
Cuts at: 3267 3267
Size: 6967
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5092 5957 6255 5092
Size: 865 298 5804
Fragments arranged by size:
5804 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5630 882
Size: 617 4131 2219
Fragments arranged by size:
4131 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2379 4758 493
Size: 711 1175 2379 2702
Fragments arranged by size:
2702 2379 1175 711
BsaBI GATnn'nnATC
Cuts at: 2859 4251 6961 2859
Size: 1392 2710 2865
Fragments arranged by size:
2865 2710 1392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3818 4774 5156 5791
Size: 53 83 186 936 2285 956 382 635
Cuts at: 5791 275
Size: 1451
Fragments arranged by size:
2285 1451 956 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5400 5798 6072 6219 6630 5400
Size: 398 274 147 411 5737
Fragments arranged by size:
5737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6742
Fragments arranged by size:
6742 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3668 1186
Size: 2482 4485
BseYI C'CCAG_C
Cuts at: 6170 6666 6170
Size: 496 6471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3087 3351 3670 1163
Size: 573 1351 264 319 4460
Fragments arranged by size:
4460 1351 573 319 264
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4417 5178 6206 1540
Size: 275 186 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3425 3539 5028 5113 6184 3425
Size: 114 1489 85 1071 4208
Fragments arranged by size:
4208 1489 1071 114 85
BsmI GAATG_Cn'
Cuts at: 3096 3347 3760 4478 4571 3096
Size: 251 413 718 93 5492
Fragments arranged by size:
5492 718 413 251 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3425 3539 4115 4286 5028 5113 6184
Size: 399 1635 114 576 171 742 85 1071
Cuts at: 6184 1391
Size: 2174
Fragments arranged by size:
2174 1635 1071 742 576 399 171 114
85
BspEI T'CCGG_A
Cuts at: 6630 6630
Size: 6967
BspHI T'CATG_A
Cuts at: 3202 3571 4856 6586 3202
Size: 369 1285 1730 3583
Fragments arranged by size:
3583 1730 1285 369
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4225 4322 844
Size: 3381 97 3489
Fragments arranged by size:
3489 3381 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4854 1394
Size: 429 3031 3507
Fragments arranged by size:
3507 3031 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3922 5462 5636 65
Size: 1183 2674 1540 174 1396
Fragments arranged by size:
2674 1540 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3388 5611 5798 1700
Size: 110 1578 2223 187 2869
Fragments arranged by size:
2869 2223 1578 187 110
BsrGI T'GTAC_A
Cuts at: 96 2513 96
Size: 2417 4550
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6967
BssSI C'ACGA_G
Cuts at: 3522 5027 6039 3522
Size: 1505 1012 4450
Fragments arranged by size:
4450 1505 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6967
BstBI TT'CG_AA
Cuts at: 5771 5771
Size: 6967
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6967
BstXI CCAn_nnnn'nTGG
Cuts at: 3218 3218
Size: 6967
BstYI r'GATC_y
Cuts at: 1972 2231 2336 2835 2965 3678 3742 4246 4297
Size: 259 105 499 130 713 64 504 51
Cuts at: 4297 4664 5049 5066 6518 6604 6616 6962 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 713 504 499 385 367 346
259 130 105 86 64 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2046 2483 3447 2046
Size: 437 964 5566
Fragments arranged by size:
5566 964 437
BtgI C'CryG_G
Cuts at: 513 1065 3937 513
Size: 552 2872 3543
Fragments arranged by size:
3543 2872 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6228
Fragments arranged by size:
6228 683 56
BtsI GCAGTG_nn'
Cuts at: 4483 5277 5297 4483
Size: 794 20 6153
Fragments arranged by size:
6153 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6967
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6932
DraI TTT'AAA
Cuts at: 4271 4271
Size: 6967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5974 809
Size: 507 4658 1802
Fragments arranged by size:
4658 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 3934 4414
Size: 54 1425 50 275 18 1597 507 480
Cuts at: 4414 5303 6932 8
Size: 889 1629 43
Fragments arranged by size:
1629 1597 1425 889 507 480 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4414 1537
Size: 275 2602 4090
Fragments arranged by size:
4090 2602 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3240 4897 1353
Size: 1887 1657 3423
Fragments arranged by size:
3423 1887 1657
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5554 5928 6074 387
Size: 1064 138 3965 374 146 1280
Fragments arranged by size:
3965 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2386 2583 3048 3332 3711 3929 5030
Size: 393 754 197 465 284 379 218 1101
Cuts at: 5030 5627 6414 6682 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1101 787 754 597 465 393 379
284 268 218 197
EcoRI G'AATT_C
Cuts at: 4276 4654 4276
Size: 378 6589
EcoRV GAT'ATC
Cuts at: 3577 3577
Size: 6967
FspI TGC'GCA
Cuts at: 5473 5473
Size: 6967
HaeII r_GCGC'y
Cuts at: 1678 6114 1678
Size: 4436 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2302 2334 2543 2575 3521
Size: 32 135 32 730 32 209 32 946
Cuts at: 3521 3553 5608 5640 5682 5714 1373
Size: 32 2055 32 42 32 2626
Fragments arranged by size:
2626 2055 946 730 209 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2084 2704 4311 4557 669
Size: 988 281 146 620 1607 246 3079
Fragments arranged by size:
3079 1607 988 620 281 246 146
HindIII A'AGCT_T
Cuts at: 748 2105 748
Size: 1357 5610
HpaI GTT'AAC
Cuts at: 4557 4557
Size: 6967
KpnI G_GTAC'C
Cuts at: 4292 4292
Size: 6967
MfeI C'AATT_G
Cuts at: 3898 4566 3898
Size: 668 6299
MluI A'CGCG_T
Cuts at: 6843 6843
Size: 6967
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3213 3542 6081 6265 1305
Size: 1908 329 2539 184 2007
Fragments arranged by size:
2539 2007 1908 329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3429 3936 10
Size: 54 1425 343 1597 507 3041
Fragments arranged by size:
3041 1597 1425 507 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2148 2352 2732 3301 3303 4925
Size: 648 976 6 204 380 569 2 1622
Cuts at: 4925 5284 5443 518
Size: 359 159 2042
Fragments arranged by size:
2042 1622 976 648 569 380 359 204
159 6 2
MspA1I CmG'CkG
Cuts at: 1606 2363 4383 5060 6208 6453 1606
Size: 757 2020 677 1148 245 2120
Fragments arranged by size:
2120 2020 1148 757 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6415
NdeI CA'TA_TG
Cuts at: 387 3380 387
Size: 2993 3974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6967
NotI GC'GGCC_GC
Cuts at: 4414 4414
Size: 6967
NsiI A_TGCA'T
Cuts at: 3173 3173
Size: 6967
NspI r_CATG'y
Cuts at: 1132 1229 2903 3171 3175 3306 4001 4325 5870
Size: 97 1674 268 4 131 695 324 1545
Cuts at: 5870 1132
Size: 2229
Fragments arranged by size:
2229 1674 1545 695 324 268 131 97
4
PasI CC'CwG_GG
Cuts at: 1348 4129 1348
Size: 2781 4186
PciI A'CATG_T
Cuts at: 2899 3302 3997 5866 2899
Size: 403 695 1869 4000
Fragments arranged by size:
4000 1869 695 403
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6967
PmeI GTTT'AAAC
Cuts at: 4271 4271
Size: 6967
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 6549 6581 5036
Size: 32 1481 32 5422
Fragments arranged by size:
5422 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6967
PsiI TTA'TAA
Cuts at: 2715 3159 4537 2715
Size: 444 1378 5145
Fragments arranged by size:
5145 1378 444
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4133 4165 4133
Size: 32 6935
PstI C_TGCA'G
Cuts at: 830 4319 830
Size: 3489 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6967
PvuII CAG'CTG
Cuts at: 2363 2363
Size: 6967
SalI G'TCGA_C
Cuts at: 1936 4309 1936
Size: 2373 4594
SbfI CC_TGCA'GG
Cuts at: 4319 4319
Size: 6967
ScaI AGT'ACT
Cuts at: 3146 5215 6927 3146
Size: 2069 1712 3186
Fragments arranged by size:
3186 2069 1712
SfcI C'TryA_G
Cuts at: 826 1045 3798 3975 4119 4315 5450 6131 6322
Size: 219 2753 177 144 196 1135 681 191
Cuts at: 6322 826
Size: 1471
Fragments arranged by size:
2753 1471 1135 681 219 196 191 177
144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6967
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6967
SmaI CCC'GGG
Cuts at: 1872 2014 4294 1872
Size: 142 2280 4545
Fragments arranged by size:
4545 2280 142
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3737 5071 5972 6234 820
Size: 197 926 1794 1334 901 262 1553
Fragments arranged by size:
1794 1553 1334 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4758 493
Size: 4265 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6967
SphI G_CATG'C
Cuts at: 1132 3175 4325 1132
Size: 2043 1150 3774
Fragments arranged by size:
3774 2043 1150
SspI AAT'ATT
Cuts at: 5 52 4891 5
Size: 47 4839 2081
Fragments arranged by size:
4839 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6967
StyI C'CwwG_G
Cuts at: 513 1065 2068 2245 2332 3025 3712 4263 4406
Size: 552 1003 177 87 693 687 551 143
Cuts at: 4406 513
Size: 3074
Fragments arranged by size:
3074 1003 693 687 552 551 177 143
87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2156 4994 5011 5164 5349 5794 325
Size: 1255 576 2838 17 153 185 445 1498
Fragments arranged by size:
2838 1498 1255 576 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2344 2513 3144
Size: 274 80 33 51 1443 367 169 631
Cuts at: 3144 3275 3307 5213 6925 96
Size: 131 32 1906 1712 138
Fragments arranged by size:
1906 1712 1443 631 367 274 169 138
131 80 51 33 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 3060 3092 6927 6959 2287
Size: 32 741 32 3835 32 2295
Fragments arranged by size:
3835 2295 741 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6967
XbaI T'CTAG_A
Cuts at: 4303 4303
Size: 6967
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6967
XmaI C'CCGG_G
Cuts at: 1870 2012 4292 1870
Size: 142 2280 4545
Fragments arranged by size:
4545 2280 142
XmnI GAAnn'nnTTC
Cuts at: 5096 5096
Size: 6967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4775 276
Size: 53 83 186 936 3241 2468
Fragments arranged by size:
3241 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI