Restriction map for
pFN21AE0355
(Circular) (Six-base) MAPSORT of: pFN21AE0355.seq Check: 8451 from: 1 to: 6598
pFN21AE0355 6598 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2838 2838
Size: 6598
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3173 4408 278
Size: 53 83 186 936 1637 1235 2468
Fragments arranged by size:
2468 1637 1235 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2255 2346 3261 3941 1937
Size: 318 91 915 680 4594
Fragments arranged by size:
4594 915 680 318 91
Acc65I G'GTAC_C
Cuts at: 3807 3919 3807
Size: 112 6486
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2306 2390 2501 3248 4661 6045 6313
Size: 608 66 84 111 747 1413 1384 268
Cuts at: 6313 1632
Size: 1917
Fragments arranged by size:
1917 1413 1384 747 608 268 111 84
66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 5497 6474 1203
Size: 4294 977 1327
Fragments arranged by size:
4294 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 3560 5327 3560
Size: 1767 4831
AleI CACnn'nnGTG
Cuts at: 1166 2233 2340 1166
Size: 1067 107 5424
Fragments arranged by size:
5424 1067 107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3125 3157 3125
Size: 32 6566
AlwNI CAG_nnn'CTG
Cuts at: 2239 2479 2866 3163 3226 4341 5913 2239
Size: 240 387 297 63 1115 1572 2924
Fragments arranged by size:
2924 1572 1115 387 297 240 63
ApaI G_GGCC'C
Cuts at: 2251 2716 3678 2251
Size: 465 962 5171
Fragments arranged by size:
5171 962 465
ApaLI G'TGCA_C
Cuts at: 3398 4655 5811 3398
Size: 1257 1156 4185
Fragments arranged by size:
4185 1257 1156
ApoI r'AATT_y
Cuts at: 1493 3907 4133 4285 6394 1493
Size: 2414 226 152 2109 1697
Fragments arranged by size:
2414 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 2100 2387 2598 3017 3408 3879 3923
Size: 73 157 287 211 419 391 471 44
Cuts at: 3923 1870
Size: 4545
Fragments arranged by size:
4545 471 419 391 287 211 157 73
44
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3704 3807 3919 5374 618
Size: 325 217 627 1917 103 112 1455 1842
Fragments arranged by size:
1917 1842 1455 627 325 217 112 103
BanII G_rGCy'C
Cuts at: 1391 2251 2716 3024 3213 3409 3441 3552 3678
Size: 860 465 308 189 196 32 111 126
Cuts at: 3678 3917 1391
Size: 239 4072
Fragments arranged by size:
4072 860 465 308 239 196 189 126
111 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2543 3236 3642 928
Size: 971 644 693 406 3884
Fragments arranged by size:
3884 971 693 644 406
BbvCI CC'TCA_GC
Cuts at: 3743 3759 3743
Size: 16 6582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2662 2696 4789 4823 1903
Size: 34 725 34 2093 34 3678
Fragments arranged by size:
3678 2093 725 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2913 4490 5700 2913
Size: 1577 1210 3811
Fragments arranged by size:
3811 1577 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6598
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2069 2504
Size: 107 122 71 631 605 343 54 435
Cuts at: 2504 3413 3512 5209 136
Size: 909 99 1697 1525
Fragments arranged by size:
1697 1525 909 631 605 435 343 122
107 99 71 54
BglII A'GATC_T
Cuts at: 2391 6593 2391
Size: 4202 2396
BlpI GC'TnA_GC
Cuts at: 2834 4015 2834
Size: 1181 5417
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2251 2716 3402 3678 3856 4659 5815
Size: 409 52 465 686 276 178 803 1156
Cuts at: 5815 1790
Size: 2573
Fragments arranged by size:
2573 1156 803 686 465 409 276 178
52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 4329 5287
Size: 416 53 83 399 24 828 2084 958
Cuts at: 5287 442
Size: 1753
Fragments arranged by size:
2084 1753 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2228 1056
Size: 1172 5426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2384 2476 2384
Size: 92 6506
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2471 2597 2630 2753 3173 3587 3611
Size: 1022 210 126 33 123 420 414 24
Cuts at: 3611 3806 5258 1239
Size: 195 1452 2579
Fragments arranged by size:
2579 1452 1022 420 414 210 195 126
123 33 24
Bpu10I CC'TnA_GC
Cuts at: 3422 3743 3759 3857 3422
Size: 321 16 98 6163
Fragments arranged by size:
6163 321 98 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 4723 5588 5886 2765
Size: 1958 865 298 3477
Fragments arranged by size:
3477 1958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2827 3093 5261 882
Size: 617 1328 266 2168 2219
Fragments arranged by size:
2219 2168 1328 617 266
BsaAI yAC'GTr
Cuts at: 493 1204 3865 4389 493
Size: 711 2661 524 2702
Fragments arranged by size:
2702 2661 711 524
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2444 3170 4405 4787
Size: 53 83 186 936 911 726 1235 382
Cuts at: 4787 5422 275
Size: 635 1451
Fragments arranged by size:
1451 1235 936 911 726 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5031 5429 5703 5850 6261 5031
Size: 398 274 147 411 5368
Fragments arranged by size:
5368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 1178
Size: 30 165 30 723 30 5620
Fragments arranged by size:
5620 723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2468 2618 2642 3257 3476 3760 3803
Size: 954 328 150 24 615 219 284 43
Cuts at: 3803 3836 1186
Size: 33 3948
Fragments arranged by size:
3948 954 615 328 284 219 150 43
33 24
BseYI C'CCAG_C
Cuts at: 2183 2319 2519 3023 3213 5801 6297 2183
Size: 136 200 504 190 2588 496 2484
Fragments arranged by size:
2588 2484 504 496 200 190 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6025
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 2262 2332 2856 4048 4809
Size: 275 186 18 243 70 524 1192 761
Cuts at: 4809 5837 1540
Size: 1028 2301
Fragments arranged by size:
2301 1192 1028 761 524 275 243 186
70 18
BsiHKAI G_wGCw'C
Cuts at: 2885 3402 3441 3552 4659 4744 5815 2885
Size: 517 39 111 1107 85 1071 3668
Fragments arranged by size:
3668 1107 1071 517 111 85 39
BsmI GAATG_Cn'
Cuts at: 3454 4109 4202 3454
Size: 655 93 5850
Fragments arranged by size:
5850 655 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2199 2251 2716 2885 3024 3213 3402
Size: 399 409 52 465 169 139 189 189
Cuts at: 3402 3409 3441 3552 3678 3856 3917 4659 4744
Size: 7 32 111 126 178 61 742 85
Cuts at: 4744 5815 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 465 409 399 189 189
178 169 139 126 111 85 61 52
32 7
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 4487 6217 4487
Size: 1730 4868
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2838 3723 3953 844
Size: 1994 885 230 3489
Fragments arranged by size:
3489 1994 885 230
BsrBI CCG'CTC
Cuts at: 1394 1823 3178 3750 4485 1394
Size: 429 1355 572 735 3507
Fragments arranged by size:
3507 1355 735 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2998 5093 5267 65
Size: 1183 1750 2095 174 1396
Fragments arranged by size:
2095 1750 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2087 2094 2327 2634 5242 5429 1700
Size: 110 277 7 233 307 2608 187 2869
Fragments arranged by size:
2869 2608 307 277 233 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 2889 3387 96
Size: 2793 498 3307
Fragments arranged by size:
3307 2793 498
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 2807 3800 4658 5670 2807
Size: 993 858 1012 3735
Fragments arranged by size:
3735 1012 993 858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6598
BstBI TT'CG_AA
Cuts at: 3294 5402 3294
Size: 2108 4490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6598
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2080
Size: 6598
BstYI r'GATC_y
Cuts at: 1972 2391 3246 3928 4295 4680 4697 6149 6235
Size: 419 855 682 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 855 682 419 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2347 2347
Size: 6598
Bsu36I CC'TnA_GG
Cuts at: 2123 2867 3501 2123
Size: 744 634 5220
Fragments arranged by size:
5220 744 634
BtgI C'CryG_G
Cuts at: 513 1065 2004 3035 3081 3193 3234 513
Size: 552 939 1031 46 112 41 3877
Fragments arranged by size:
3877 1031 939 552 112 46 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3670 487
Size: 683 56 2444 3415
Fragments arranged by size:
3415 2444 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 2885 3842 4114 4908 4928 2240
Size: 645 957 272 794 20 3910
Fragments arranged by size:
3910 957 794 645 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3448 3483 529
Size: 35 2884 35 3644
Fragments arranged by size:
3644 2884 35 35
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2259
Size: 54 1425 50 275 18 177 9 243
Cuts at: 2259 2329 2756 3891 4045 4934 6563 8
Size: 70 427 1135 154 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 427 275 243 177
154 70 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2016 2259 2329 4045 1537
Size: 275 204 243 70 1716 4090
Fragments arranged by size:
4090 1716 275 243 204 70
EarI CTCTTCn'nnn_
Cuts at: 1353 4528 1353
Size: 3175 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5185 5559 5705 387
Size: 1064 138 3596 374 146 1280
Fragments arranged by size:
3596 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3439 3550 3439
Size: 111 6487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2261 2306 2390 2471 2501 2597
Size: 393 608 21 45 84 81 30 96
Cuts at: 2597 2630 2753 3173 3248 3587 3611 3806 4661
Size: 33 123 420 75 339 24 195 855
Cuts at: 4661 5258 6045 6313 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 855 787 608 597 420 393 339
268 195 123 96 84 81 75 45
33 30 24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3893 3893
Size: 6598
EcoO109I rG'GnC_Cy
Cuts at: 2247 2713 2797 3148 3675 2247
Size: 466 84 351 527 5170
Fragments arranged by size:
5170 527 466 351 84
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FspI TGC'GCA
Cuts at: 5104 5104
Size: 6598
HaeII r_GCGC'y
Cuts at: 1678 2052 2635 2898 5745 1678
Size: 374 583 263 2847 2531
Fragments arranged by size:
2847 2531 583 374 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2539 2571 3098 3130 3546
Size: 32 135 32 967 32 527 32 416
Cuts at: 3546 3578 5239 5271 5313 5345 1373
Size: 32 1661 32 42 32 2626
Fragments arranged by size:
2626 1661 967 527 416 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2256 3942 4188 669
Size: 988 281 318 1686 246 3079
Fragments arranged by size:
3079 1686 988 318 281 246
HindIII A'AGCT_T
Cuts at: 748 2057 2267 2402 748
Size: 1309 210 135 4944
Fragments arranged by size:
4944 1309 210 135
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3811 3923 3811
Size: 112 6486
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3094 3750 5712 5896 1305
Size: 1789 656 1962 184 2007
Fragments arranged by size:
2007 1962 1789 656 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3893 10
Size: 54 1425 343 2061 2715
Fragments arranged by size:
2715 2061 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2233 2340 4556 4915 5074 518
Size: 648 1067 107 2216 359 159 2042
Fragments arranged by size:
2216 2042 1067 648 359 159 107
MspA1I CmG'CkG
Cuts at: 1606 2012 2041 2306 2563 2710 3037 3217 3226
Size: 406 29 265 257 147 327 180 9
Cuts at: 3226 3236 3311 3327 3364 3695 3763 4014 4691
Size: 10 75 16 37 331 68 251 677
Cuts at: 4691 5839 6084 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 406 331 327 265 257
251 245 180 147 75 68 37 29
16 10 9
NaeI GCC'GGC
Cuts at: 2329 2636 2329
Size: 307 6291
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5107
Fragments arranged by size:
5107 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NgoMIV G'CCGG_C
Cuts at: 2327 2634 2327
Size: 307 6291
NheI G'CTAG_C
Cuts at: 1052 2224 1052
Size: 1172 5426
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 3007 3956 5501 1132
Size: 97 1778 949 1545 2229
Fragments arranged by size:
2229 1778 1545 949 97
PasI CC'CwG_GG
Cuts at: 1348 3100 1348
Size: 1752 4846
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PfoI T'CCnGG_A
Cuts at: 2646 2730 3564 2646
Size: 84 834 5680
Fragments arranged by size:
5680 834 84
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2797 3148 2797
Size: 351 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspOMI G'GGCC_C
Cuts at: 2247 2712 3674 2247
Size: 465 962 5171
Fragments arranged by size:
5171 962 465
PstI C_TGCA'G
Cuts at: 830 2046 2305 2469 2880 3222 3694 3849 3950
Size: 1216 259 164 411 342 472 155 101
Cuts at: 3950 830
Size: 3478
Fragments arranged by size:
3478 1216 472 411 342 259 164 155
101
PvuI CG_AT'CG
Cuts at: 2001 2856 2001
Size: 855 5743
PvuII CAG'CTG
Cuts at: 2306 3217 3226 3311 3695 3763 2306
Size: 911 9 85 384 68 5141
Fragments arranged by size:
5141 911 384 85 68 9
SacI G_AGCT'C
Cuts at: 3441 3552 3441
Size: 111 6487
SacII CC_GC'GG
Cuts at: 3038 3237 3038
Size: 199 6399
SalI G'TCGA_C
Cuts at: 1936 2254 3940 1936
Size: 318 1686 4594
Fragments arranged by size:
4594 1686 318
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 2042 2301 2465 2558 2876 3218 3442
Size: 219 997 259 164 93 318 342 224
Cuts at: 3442 3690 3845 3946 5081 5762 5953 826
Size: 248 155 101 1135 681 191 1471
Fragments arranged by size:
1471 1135 997 681 342 318 259 248
224 219 191 164 155 101 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 2015 3512 2015
Size: 1497 5101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 2102 2600 3410 3881 3925 1872
Size: 230 498 810 471 44 4545
Fragments arranged by size:
4545 810 498 471 230 44
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2780 4702 5603 5865 820
Size: 197 926 837 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 837 262 197
SnaBI TAC'GTA
Cuts at: 493 4389 493
Size: 3896 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6598
SphI G_CATG'C
Cuts at: 1132 3007 3956 1132
Size: 1875 949 3774
Fragments arranged by size:
3774 1875 949
SspI AAT'ATT
Cuts at: 5 52 4522 5
Size: 47 4470 2081
Fragments arranged by size:
4470 2081 47
StuI AGG'CCT
Cuts at: 1517 2459 3300 3888 1517
Size: 942 841 588 4227
Fragments arranged by size:
4227 942 841 588
StyI C'CwwG_G
Cuts at: 513 1065 2004 3318 3507 3894 4037 513
Size: 552 939 1314 189 387 143 3074
Fragments arranged by size:
3074 1314 939 552 387 189 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4625 4642 4795 4980 5425 325
Size: 1255 3045 17 153 185 445 1498
Fragments arranged by size:
3045 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2889 3387 4844
Size: 274 80 33 51 1443 912 498 1457
Cuts at: 4844 6556 96
Size: 1712 138
Fragments arranged by size:
1712 1457 1443 912 498 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 3607 3639 6558 6590 2126
Size: 32 1449 32 2919 32 2134
Fragments arranged by size:
2919 2134 1449 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6598
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6598
XmaI C'CCGG_G
Cuts at: 1870 2100 2598 3408 3879 3923 1870
Size: 230 498 810 471 44 4545
Fragments arranged by size:
4545 810 498 471 230 44
XmnI GAAnn'nnTTC
Cuts at: 4727 4727
Size: 6598
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3171 4406 276
Size: 53 83 186 936 1637 1235 2468
Fragments arranged by size:
2468 1637 1235 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
DraIII EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI
SexAI SfoI SrfI SwaI XcmI