Restriction map for
pFN21AE0350
(Circular) (Six-base) MAPSORT of: pFN21AE0350.seq Check: 5669 from: 1 to: 6946
pFN21AE0350 6946 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4756 278
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3944 4289 1937
Size: 2007 345 4594
Fragments arranged by size:
4594 2007 345
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 2106 2816 3902 5009 6393 6661 1632
Size: 417 57 710 1086 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1107 1086 710 417 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 3567 3645 5845 6822 1203
Size: 2364 78 2200 977 1327
Fragments arranged by size:
2364 2200 1327 977 78
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 5675 5675
Size: 6946
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2944 1184
Size: 1760 5186
AlwNI CAG_nnn'CTG
Cuts at: 4689 6261 4689
Size: 1572 5374
ApaLI G'TGCA_C
Cuts at: 5003 6159 5003
Size: 1156 5790
ApoI r'AATT_y
Cuts at: 1493 2737 3412 3793 3840 4077 4255 4481 4633
Size: 1244 675 381 47 237 178 226 152
Cuts at: 4633 6742 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1244 675 381 237 226 178
152 47
AseI AT'TA_AT
Cuts at: 160 1032 3198 5500 160
Size: 872 2166 2302 1606
Fragments arranged by size:
2302 2166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 4271 1870
Size: 73 2328 4545
Fragments arranged by size:
4545 2328 73
BamHI G'GATC_C
Cuts at: 4276 4643 4276
Size: 367 6579
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2790 4267 5722 618
Size: 325 217 627 1003 1477 1455 1842
Fragments arranged by size:
1842 1477 1455 1003 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3276 3739 3984 4265 1391
Size: 1885 463 245 281 4072
Fragments arranged by size:
4072 1885 463 281 245
BbeI G_GCGC'C
Cuts at: 2794 2794
Size: 6946
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2249 3188 3499 4009 928
Size: 971 350 939 311 510 3865
Fragments arranged by size:
3865 971 939 510 350 311
BbvCI CC'TCA_GC
Cuts at: 4224 4224
Size: 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5137 5171 1903
Size: 34 50 34 3116 34 3678
Fragments arranged by size:
3678 3116 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4838 6048 4838
Size: 1210 5736
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BfrBI ATG'CAT
Cuts at: 2439 3055 2439
Size: 616 6330
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5557 136
Size: 107 122 71 631 605 3885 1525
Fragments arranged by size:
3885 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2139 6941 2139
Size: 4802 2144
BlpI GC'TnA_GC
Cuts at: 3270 4025 4363 3270
Size: 755 338 5853
Fragments arranged by size:
5853 755 338
Bme1580I G_kGCm'C
Cuts at: 1790 2434 5007 6163 1790
Size: 644 2573 1156 2573
Fragments arranged by size:
2573 2573 1156 644
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4677 5635 442
Size: 416 53 83 399 24 3260 958 1753
Fragments arranged by size:
3260 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3461 3753 5606 1239
Size: 968 1254 292 1853 2579
Fragments arranged by size:
2579 1853 1254 968 292
Bpu10I CC'TnA_GC
Cuts at: 2691 4224 2691
Size: 1533 5413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2745 3497 4142 5071 5936 6234 2745
Size: 752 645 929 865 298 3457
Fragments arranged by size:
3457 929 865 752 645 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 882
Size: 617 4110 2219
Fragments arranged by size:
4110 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2444 4737 493
Size: 711 1240 2293 2702
Fragments arranged by size:
2702 2293 1240 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2791 3100 4753 5135
Size: 53 83 186 936 1258 309 1653 382
Cuts at: 5135 5770 275
Size: 635 1451
Fragments arranged by size:
1653 1451 1258 936 635 382 309 186
83 53
BsaWI w'CCGG_w
Cuts at: 4149 5379 5777 6051 6198 6609 4149
Size: 1230 398 274 147 411 4486
Fragments arranged by size:
4486 1230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 3242 3272 3557
Size: 30 165 30 777 30 1032 30 285
Cuts at: 3557 3587 1178
Size: 30 4537
Fragments arranged by size:
4537 1032 777 285 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2550 2797 3313 3565 4103 1186
Size: 1364 247 516 252 538 4029
Fragments arranged by size:
4029 1364 538 516 252 247
BseYI C'CCAG_C
Cuts at: 2023 6149 6645 2023
Size: 4126 496 2324
Fragments arranged by size:
4126 2324 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 3993 1163
Size: 573 642 1615 4116
Fragments arranged by size:
4116 1615 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3669 4031 5007 5092 6163 3669
Size: 362 976 85 1071 4452
Fragments arranged by size:
4452 1071 976 362 85
BsmI GAATG_Cn'
Cuts at: 3758 4457 4550 3758
Size: 699 93 6154
Fragments arranged by size:
6154 699 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2434 3276 3669 3739 3984 4031 4265
Size: 399 644 842 393 70 245 47 234
Cuts at: 4265 5007 5092 6163 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 842 742 644 399 393 245
234 85 70 47
BspEI T'CCGG_A
Cuts at: 4149 6609 4149
Size: 2460 4486
BspHI T'CATG_A
Cuts at: 2158 3750 3762 4084 4835 6565 2158
Size: 1592 12 322 751 1730 2539
Fragments arranged by size:
2539 1730 1592 751 322 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4301 844
Size: 3457 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 5441 5615 65
Size: 1183 810 3383 174 1396
Fragments arranged by size:
3383 1396 1183 810 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2787 5590 5777 1700
Size: 110 977 2803 187 2869
Fragments arranged by size:
2869 2803 977 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6946
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 5006 6018 5006
Size: 1012 5934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3351 794
Size: 2557 4389
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6946
BstYI r'GATC_y
Cuts at: 1972 2139 3387 4276 4643 5028 5045 6497 6583
Size: 167 1248 889 367 385 17 1452 86
Cuts at: 6583 6595 6941 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1248 889 385 367 346 167
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3832 487
Size: 683 56 2606 3601
Fragments arranged by size:
3601 2606 683 56
BtsI GCAGTG_nn'
Cuts at: 3349 4076 4462 5256 5276 3349
Size: 727 386 794 20 5019
Fragments arranged by size:
5019 794 727 386 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3541 3576 529
Size: 35 2977 35 3899
Fragments arranged by size:
3899 2977 35 35
DraI TTT'AAA
Cuts at: 3798 4250 3798
Size: 452 6494
DraIII CAC_nnn'GTG
Cuts at: 2270 2270
Size: 6946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2993 5953 809
Size: 507 1677 2960 1802
Fragments arranged by size:
2960 1802 1677 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3292 3562 4393
Size: 54 1425 50 275 18 1462 270 831
Cuts at: 4393 5282 6911 8
Size: 889 1629 43
Fragments arranged by size:
1629 1462 1425 889 831 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 4876 1353
Size: 672 2851 3423
Fragments arranged by size:
3423 2851 672
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5533 5907 6053 387
Size: 1064 138 3944 374 146 1280
Fragments arranged by size:
3944 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2106 2207 2816 3461 3753 3902
Size: 393 417 57 101 609 645 292 149
Cuts at: 3902 5009 5606 6393 6661 1239
Size: 1107 597 787 268 1524
Fragments arranged by size:
1524 1107 787 645 609 597 417 393
292 268 149 101 57
EcoNI CCTnn'n_nnAGG
Cuts at: 3248 3680 3248
Size: 432 6514
EcoO109I rG'GnC_Cy
Cuts at: 2581 2765 3169 4130 2581
Size: 184 404 961 5397
Fragments arranged by size:
5397 961 404 184
EcoRI G'AATT_C
Cuts at: 4255 4633 4255
Size: 378 6568
EcoRV GAT'ATC
Cuts at: 3778 4147 3778
Size: 369 6577
FspI TGC'GCA
Cuts at: 2057 5452 2057
Size: 3395 3551
HaeII r_GCGC'y
Cuts at: 1678 2794 6093 1678
Size: 1116 3299 2531
Fragments arranged by size:
3299 2531 1116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 2180 2212 2363
Size: 32 135 32 568 32 8 32 151
Cuts at: 2363 2395 2530 2562 3533 3565 3764 3796 5587
Size: 32 135 32 971 32 199 32 1791
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1791 971 568 199 151 135 135
42 32 32 32 32 32 32 32
32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 4290 4536 669
Size: 988 281 2352 246 3079
Fragments arranged by size:
3079 2352 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6946
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KasI G'GCGC_C
Cuts at: 2790 2790
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2348 3736 6060 6244 1305
Size: 1043 1388 2324 184 2007
Fragments arranged by size:
2324 2007 1388 1043 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3564 10
Size: 54 1425 343 1732 3392
Fragments arranged by size:
3392 1732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2301 2916 3060 3458 3662 3851 4904
Size: 648 1135 615 144 398 204 189 1053
Cuts at: 4904 5263 5422 518
Size: 359 159 2042
Fragments arranged by size:
2042 1135 1053 648 615 398 359 204
189 159 144
MspA1I CmG'CkG
Cuts at: 1606 2027 4362 5039 6187 6432 1606
Size: 421 2335 677 1148 245 2120
Fragments arranged by size:
2335 2120 1148 677 421 245
NarI GG'CG_CC
Cuts at: 2791 2791
Size: 6946
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6394
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NsiI A_TGCA'T
Cuts at: 2441 3057 2441
Size: 616 6330
NspI r_CATG'y
Cuts at: 1132 1229 2458 3269 3571 3649 4304 5849 1132
Size: 97 1229 811 302 78 655 1545 2229
Fragments arranged by size:
2229 1545 1229 811 655 302 97 78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6946
PciI A'CATG_T
Cuts at: 3567 3645 5845 3567
Size: 78 2200 4668
Fragments arranged by size:
4668 2200 78
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3428 1108
Size: 2320 4626
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5015 5047 6528 6560 5015
Size: 32 1481 32 5401
Fragments arranged by size:
5401 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2581 2765 3169 2581
Size: 184 404 6358
Fragments arranged by size:
6358 404 184
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 2889 4516 2889
Size: 1627 5319
PstI C_TGCA'G
Cuts at: 830 2897 4298 830
Size: 2067 1401 3478
Fragments arranged by size:
3478 2067 1401
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 2027 2027
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SbfI CC_TGCA'GG
Cuts at: 4298 4298
Size: 6946
ScaI AGT'ACT
Cuts at: 3821 5194 6906 3821
Size: 1373 1712 3861
Fragments arranged by size:
3861 1712 1373
SexAI A'CCwGG_T
Cuts at: 3243 4196 3243
Size: 953 5993
SfcI C'TryA_G
Cuts at: 826 1045 2893 2998 3147 3815 3945 4294 5429
Size: 219 1848 105 149 668 130 349 1135
Cuts at: 5429 6110 6301 826
Size: 681 191 1471
Fragments arranged by size:
1848 1471 1135 681 668 349 219 191
149 130 105
SfoI GGC'GCC
Cuts at: 2792 2792
Size: 6946
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 2787 1700
Size: 1087 5859
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2760 3512 4121 5050 5951 6213
Size: 197 926 817 752 609 929 901 262
Cuts at: 6213 820
Size: 1553
Fragments arranged by size:
1553 929 926 901 817 752 609 262
197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 3269 4304 1132
Size: 2137 1035 3774
Fragments arranged by size:
3774 2137 1035
SspI AAT'ATT
Cuts at: 5 52 3209 3448 4870 5
Size: 47 3157 239 1422 2081
Fragments arranged by size:
3157 2081 1422 239 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6946
StyI C'CwwG_G
Cuts at: 513 1065 2233 3975 4385 513
Size: 552 1168 1742 410 3074
Fragments arranged by size:
3074 1742 1168 552 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2924 3758 4973 4990 5143 5328 5773
Size: 1255 1344 834 1215 17 153 185 445
Cuts at: 5773 325
Size: 1498
Fragments arranged by size:
1498 1344 1255 1215 834 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3819 5192 6904
Size: 274 80 33 51 1443 1842 1373 1712
Cuts at: 6904 96
Size: 138
Fragments arranged by size:
1842 1712 1443 1373 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 3557 3589 6906 6938 2163
Size: 32 1362 32 3317 32 2171
Fragments arranged by size:
3317 2171 1362 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2194 1316
Size: 878 6068
XbaI T'CTAG_A
Cuts at: 3883 4282 3883
Size: 399 6547
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 3449 5075 3449
Size: 1626 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4754 276
Size: 53 83 186 936 3220 2468
Fragments arranged by size:
3220 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SrfI SwaI XcmI