Restriction map for
pFN21AE0349
(Circular) (Six-base) MAPSORT of: pFN21AE0349.seq Check: 4086 from: 1 to: 6859
pFN21AE0349 6859 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4202 1937
Size: 2265 4594
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2802 3782 3798 3825 4922 6306 6574 1632
Size: 1170 980 16 27 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1170 1097 980 268 27 16
AfeI AGC'GCT
Cuts at: 3395 3395
Size: 6859
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 2655 5758 6735 1203
Size: 1452 3103 977 1327
Fragments arranged by size:
3103 1452 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6859
AlwNI CAG_nnn'CTG
Cuts at: 2216 2572 4602 6174 2216
Size: 356 2030 1572 2901
Fragments arranged by size:
2901 2030 1572 356
ApaLI G'TGCA_C
Cuts at: 4916 6072 4916
Size: 1156 5703
ApoI r'AATT_y
Cuts at: 1493 2252 2713 4131 4168 4394 4546 6655 1493
Size: 759 461 1418 37 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 759 461 226 152 37
AseI AT'TA_AT
Cuts at: 160 1032 5413 160
Size: 872 4381 1606
Fragments arranged by size:
4381 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 3216 4184 1870
Size: 73 1273 968 4545
Fragments arranged by size:
4545 1273 968 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2818 2851 2818
Size: 33 6826
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2159 2441 3280 4180 5635
Size: 325 217 627 372 282 839 900 1455
Cuts at: 5635 618
Size: 1842
Fragments arranged by size:
1842 1455 900 839 627 372 325 282
217
BanII G_rGCy'C
Cuts at: 1391 4178 1391
Size: 2787 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3786 928
Size: 971 1887 4001
Fragments arranged by size:
4001 1887 971
BbvCI CC'TCA_GC
Cuts at: 4010 4010
Size: 6859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5050 5084 1903
Size: 34 3113 34 3678
Fragments arranged by size:
3678 3113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2111 2961 4751 5961 2111
Size: 850 1790 1210 3009
Fragments arranged by size:
3009 1790 1210 850
BclI T'GATC_A
Cuts at: 1548 2580 3264 1548
Size: 1032 684 5143
Fragments arranged by size:
5143 1032 684
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 5470 136
Size: 107 122 71 631 605 370 3428 1525
Fragments arranged by size:
3428 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 6854 6854
Size: 6859
BlpI GC'TnA_GC
Cuts at: 4276 4276
Size: 6859
Bme1580I G_kGCm'C
Cuts at: 1790 2446 2593 3484 4920 6076 1790
Size: 656 147 891 1436 1156 2573
Fragments arranged by size:
2573 1436 1156 891 656 147
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6859
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3350 3842 4590
Size: 416 53 83 399 24 1933 492 748
Cuts at: 4590 5548 442
Size: 958 1753
Fragments arranged by size:
1933 1753 958 748 492 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3694 3694
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2238 5519 1239
Size: 999 3281 2579
Fragments arranged by size:
3281 2579 999
Bpu10I CC'TnA_GC
Cuts at: 4010 4010
Size: 6859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4984 5849 6147 4984
Size: 865 298 5696
Fragments arranged by size:
5696 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3737 4023 5522 882
Size: 617 2238 286 1499 2219
Fragments arranged by size:
2238 2219 1499 617 286
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 3813 6853 3813
Size: 3040 3819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4666 5048 5683 275
Size: 53 83 186 936 3133 382 635 1451
Fragments arranged by size:
3133 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2310 2528 5292 5690 5964 6111 6522 2310
Size: 218 2764 398 274 147 411 2647
Fragments arranged by size:
2764 2647 411 398 274 218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2523 2553 3592 3622 3881
Size: 30 165 30 1120 30 1039 30 259
Cuts at: 3881 3911 1178
Size: 30 4126
Fragments arranged by size:
4126 1120 1039 259 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3851 4033 4058 1186
Size: 2665 182 25 3987
Fragments arranged by size:
3987 2665 182 25
BseYI C'CCAG_C
Cuts at: 2812 6062 6558 2812
Size: 3250 496 3113
Fragments arranged by size:
3250 3113 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2873 1163
Size: 573 1137 5149
Fragments arranged by size:
5149 1137 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4309 5070 6098 1540
Size: 275 186 2308 761 1028 2301
Fragments arranged by size:
2308 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2196 3009 4920 5005 6076 2196
Size: 813 1911 85 1071 2979
Fragments arranged by size:
2979 1911 1071 813 85
BsmI GAATG_Cn'
Cuts at: 3717 4370 4463 3717
Size: 653 93 6113
Fragments arranged by size:
6113 653 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2446 2593 3009 3484 4178 4920
Size: 399 406 250 147 416 475 694 742
Cuts at: 4920 5005 6076 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 694 475 416 406 399
250 147 85
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 2577 4748 6478 2577
Size: 2171 1730 2958
Fragments arranged by size:
2958 2171 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3836 4214 844
Size: 2992 378 3489
Fragments arranged by size:
3489 2992 378
BsrBI CCG'CTC
Cuts at: 1394 1823 4746 1394
Size: 429 2923 3507
Fragments arranged by size:
3507 2923 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2031 2877 3406 5354 5528 65
Size: 1183 783 846 529 1948 174 1396
Fragments arranged by size:
1948 1396 1183 846 783 529 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5503 5690 1700
Size: 110 3693 187 2869
Fragments arranged by size:
3693 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3186 96
Size: 3090 3769
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6859
BssSI C'ACGA_G
Cuts at: 4919 5931 4919
Size: 1012 5847
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2033 2572 3013 794
Size: 1239 539 441 4640
Fragments arranged by size:
4640 1239 539 441
BstBI TT'CG_AA
Cuts at: 3628 5663 3628
Size: 2035 4824
BstEII G'GTnAC_C
Cuts at: 1184 3823 1184
Size: 2639 4220
BstYI r'GATC_y
Cuts at: 1972 2213 2428 4189 4556 4941 4958 6410 6496
Size: 241 215 1761 367 385 17 1452 86
Cuts at: 6496 6508 6854 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1761 1452 385 367 346 241 215
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3859 3859
Size: 6859
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2244 2550 3007 3117 3736 4375 5169 5189 2244
Size: 306 457 110 619 639 794 20 3914
Fragments arranged by size:
3914 794 639 619 457 306 110 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3273 3308 529
Size: 35 2709 35 4080
Fragments arranged by size:
4080 2709 35 35
DraI TTT'AAA
Cuts at: 3997 4163 3997
Size: 166 6693
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 3546 4306
Size: 54 1425 50 275 18 529 1187 760
Cuts at: 4306 5195 6824 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1187 889 760 529 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4306 1537
Size: 275 2494 4090
Fragments arranged by size:
4090 2494 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4035 4065 4789 1353
Size: 2682 30 724 3423
Fragments arranged by size:
3423 2682 724 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5446 5820 5966 387
Size: 1064 138 3857 374 146 1280
Fragments arranged by size:
3857 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2238 2802 3782 3798 3825 4922 5519
Size: 393 606 564 980 16 27 1097 597
Cuts at: 5519 6306 6574 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1097 980 787 606 597 564 393
268 27 16
EcoO109I rG'GnC_Cy
Cuts at: 2860 3793 2860
Size: 933 5926
EcoRI G'AATT_C
Cuts at: 2252 4168 4546 2252
Size: 1916 378 4565
Fragments arranged by size:
4565 1916 378
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 2137 3397 6006 1678
Size: 459 1260 2609 2531
Fragments arranged by size:
2609 2531 1260 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3109 3128 3141 3160 3881
Size: 32 135 32 1537 19 13 19 721
Cuts at: 3881 3913 5500 5532 5574 5606 1373
Size: 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 1537 721 135 42 32 32
32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 3889 4203 4449 669
Size: 988 281 1951 314 246 3079
Fragments arranged by size:
3079 1951 988 314 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6859
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 2154 4458 2154
Size: 2304 4555
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2450 2730 2789 5973 6157 1305
Size: 1145 280 59 3184 184 2007
Fragments arranged by size:
3184 2007 1145 280 184 59
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 3548 10
Size: 54 1425 343 529 1187 3321
Fragments arranged by size:
3321 1425 1187 529 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 3017 4817 5176 5335 518
Size: 648 1338 513 1800 359 159 2042
Fragments arranged by size:
2042 1800 1338 648 513 359 159
MspA1I CmG'CkG
Cuts at: 1606 4275 4952 6100 6345 1606
Size: 2669 677 1148 245 2120
Fragments arranged by size:
2669 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6307
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 2659 3016 4217 5762 1132
Size: 97 1430 357 1201 1545 2229
Fragments arranged by size:
2229 1545 1430 1201 357 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 2655 5758 2655
Size: 3103 3756
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3642 1108
Size: 2534 4325
PfoI T'CCnGG_A
Cuts at: 2454 2963 2454
Size: 509 6350
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4928 4960 6441 6473 4928
Size: 32 1481 32 5314
Fragments arranged by size:
5314 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2860 2860
Size: 6859
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 6827
PstI C_TGCA'G
Cuts at: 830 2568 3833 4211 830
Size: 1738 1265 378 3478
Fragments arranged by size:
3478 1738 1265 378
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
SalI G'TCGA_C
Cuts at: 1936 4201 1936
Size: 2265 4594
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 5107 6819 5107
Size: 1712 5147
SexAI A'CCwGG_T
Cuts at: 3657 3657
Size: 6859
SfcI C'TryA_G
Cuts at: 826 1045 2564 3829 4207 5342 6023 6214 826
Size: 219 1519 1265 378 1135 681 191 1471
Fragments arranged by size:
1519 1471 1265 1135 681 378 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6859
SmaI CCC'GGG
Cuts at: 1872 3218 4186 1872
Size: 1346 968 4545
Fragments arranged by size:
4545 1346 968
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4963 5864 6126 820
Size: 197 926 3020 901 262 1553
Fragments arranged by size:
3020 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 3016 4217 1132
Size: 1884 1201 3774
Fragments arranged by size:
3774 1884 1201
SspI AAT'ATT
Cuts at: 5 52 2182 3049 4102 4783 5
Size: 47 2130 867 1053 681 2081
Fragments arranged by size:
2130 2081 1053 867 681 47
StuI AGG'CCT
Cuts at: 1517 2535 1517
Size: 1018 5841
StyI C'CwwG_G
Cuts at: 513 1065 2783 4298 513
Size: 552 1718 1515 3074
Fragments arranged by size:
3074 1718 1515 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3268 3911 4886 4903 5056 5241 5686
Size: 1255 1688 643 975 17 153 185 445
Cuts at: 5686 325
Size: 1498
Fragments arranged by size:
1688 1498 1255 975 643 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2014 3186 5105
Size: 274 80 33 51 1443 37 1172 1919
Cuts at: 5105 6817 96
Size: 1712 138
Fragments arranged by size:
1919 1712 1443 1172 274 138 80 51
37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 3592 3624 6819 6851 2223
Size: 32 1337 32 3195 32 2231
Fragments arranged by size:
3195 2231 1337 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2843 1316
Size: 1527 5332
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 2820 2422
Size: 398 6461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6859
XmaI C'CCGG_G
Cuts at: 1870 3216 4184 1870
Size: 1346 968 4545
Fragments arranged by size:
4545 1346 968
XmnI GAAnn'nnTTC
Cuts at: 2778 2959 3624 4988 2778
Size: 181 665 1364 4649
Fragments arranged by size:
4649 1364 665 181
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI