Restriction map for
pFN21AE0344
(Circular) (Six-base) MAPSORT of: pFN21AE0344.seq Check: 5763 from: 1 to: 6826
pFN21AE0344 6826 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2670 2767 2670
Size: 97 6729
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4636 278
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2973 4169 1937
Size: 1036 1196 4594
Fragments arranged by size:
4594 1196 1036
Acc65I G'GTAC_C
Cuts at: 4147 4147
Size: 6826
AclI AA'CG_TT
Cuts at: 1563 4953 5326 1563
Size: 3390 373 3063
Fragments arranged by size:
3390 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3107 4889 6273 6541 1632
Size: 1475 1782 1384 268 1917
Fragments arranged by size:
1917 1782 1475 1384 268
AfeI AGC'GCT
Cuts at: 3686 3686
Size: 6826
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6629
AflIII A'CryG_T
Cuts at: 1203 5725 6702 1203
Size: 4522 977 1327
Fragments arranged by size:
4522 1327 977
AgeI A'CCGG_T
Cuts at: 5657 5657
Size: 6826
AhdI GACnn_n'nnGTC
Cuts at: 5555 5555
Size: 6826
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2895 1184
Size: 1711 5115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 6794
AlwNI CAG_nnn'CTG
Cuts at: 2173 2236 2574 2629 2782 2794 2875 3085 3967
Size: 63 338 55 153 12 81 210 882
Cuts at: 3967 4569 6141 2173
Size: 602 1572 2858
Fragments arranged by size:
2858 1572 882 602 338 210 153 81
63 55 12
ApaI G_GGCC'C
Cuts at: 2485 3313 3548 2485
Size: 828 235 5763
Fragments arranged by size:
5763 828 235
ApaLI G'TGCA_C
Cuts at: 2259 2999 4883 6039 2259
Size: 740 1884 1156 3046
Fragments arranged by size:
3046 1884 1156 740
ApoI r'AATT_y
Cuts at: 1493 4135 4361 4513 6622 1493
Size: 2642 226 152 2109 1697
Fragments arranged by size:
2642 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5380 160
Size: 872 4348 1606
Fragments arranged by size:
4348 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 3281 4151 1870
Size: 73 168 1170 870 4545
Fragments arranged by size:
4545 1170 870 168 73
BamHI G'GATC_C
Cuts at: 4156 4523 4156
Size: 367 6459
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3259 3294 3326 3496 3735
Size: 325 217 627 1472 35 32 170 239
Cuts at: 3735 4003 4147 5602 618
Size: 268 144 1455 1842
Fragments arranged by size:
1842 1472 1455 627 325 268 239 217
170 144 35 32
BanII G_rGCy'C
Cuts at: 1391 2015 2281 2294 2485 2595 3282 3313 3548
Size: 624 266 13 191 110 687 31 235
Cuts at: 3548 3682 3930 4145 1391
Size: 134 248 215 4072
Fragments arranged by size:
4072 687 624 266 248 235 215 191
134 110 31 13
BbeI G_GCGC'C
Cuts at: 3298 3739 3298
Size: 441 6385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 2502 2915 3095 928
Size: 971 151 452 413 180 4659
Fragments arranged by size:
4659 971 452 413 180 151
BbvCI CC'TCA_GC
Cuts at: 2432 2432
Size: 6826
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5017 5051 1903
Size: 34 3080 34 3678
Fragments arranged by size:
3678 3080 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3654 4718 5928 3654
Size: 1064 1210 4552
Fragments arranged by size:
4552 1210 1064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6826
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2106 2115 2355
Size: 107 122 71 631 605 434 9 240
Cuts at: 2355 2671 2939 3944 5437 136
Size: 316 268 1005 1493 1525
Fragments arranged by size:
1525 1493 1005 631 605 434 316 268
240 122 107 71 9
BglII A'GATC_T
Cuts at: 6821 6821
Size: 6826
BlpI GC'TnA_GC
Cuts at: 2246 2288 2684 2883 4243 2246
Size: 42 396 199 1360 4829
Fragments arranged by size:
4829 1360 396 199 42
Bme1580I G_kGCm'C
Cuts at: 1790 2263 2485 3003 3154 3227 3313 3501 3548
Size: 473 222 518 151 73 86 188 47
Cuts at: 3548 4887 6043 1790
Size: 1339 1156 2573
Fragments arranged by size:
2573 1339 1156 518 473 222 188 151
86 73 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6826
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2222 2617 3390
Size: 416 53 83 399 24 805 395 773
Cuts at: 3390 4557 5515 442
Size: 1167 958 1753
Fragments arranged by size:
1753 1167 958 805 773 416 399 395
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3974 1056
Size: 2918 3908
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2258 3031 3035 3431 3809 3923 4078
Size: 785 234 773 4 396 378 114 155
Cuts at: 4078 5486 1239
Size: 1408 2579
Fragments arranged by size:
2579 1408 785 773 396 378 234 155
114 4
Bpu10I CC'TnA_GC
Cuts at: 2432 3473 3800 2432
Size: 1041 327 5458
Fragments arranged by size:
5458 1041 327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4951 5816 6114 4951
Size: 865 298 5663
Fragments arranged by size:
5663 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 2612 5489 882
Size: 617 675 438 2877 2219
Fragments arranged by size:
2877 2219 675 617 438
BsaAI yAC'GTr
Cuts at: 493 1204 3274 4617 493
Size: 711 2070 1343 2702
Fragments arranged by size:
2702 2070 1343 711
BsaBI GATnn'nnATC
Cuts at: 2801 6820 2801
Size: 4019 2807
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2749 3295 3736 4633
Size: 53 83 186 936 1216 546 441 897
Cuts at: 4633 5015 5650 275
Size: 382 635 1451
Fragments arranged by size:
1451 1216 936 897 635 546 441 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3987 4049 5259 5657 5931 6078 6489 3987
Size: 62 1210 398 274 147 411 4324
Fragments arranged by size:
4324 1210 411 398 274 147 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3434 3464 3686 3716 4076
Size: 30 165 30 2031 30 222 30 360
Cuts at: 4076 4106 1178
Size: 30 3898
Fragments arranged by size:
3898 2031 360 222 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3764 3920 1186
Size: 2578 156 4092
Fragments arranged by size:
4092 2578 156
BseYI C'CCAG_C
Cuts at: 2254 3115 3681 3705 6029 6525 2254
Size: 861 566 24 2324 496 2555
Fragments arranged by size:
2555 2324 861 566 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2660 3980 1163
Size: 573 924 1320 4009
Fragments arranged by size:
4009 1320 924 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3234 3482 4276 5037 6065
Size: 275 186 54 1179 248 794 761 1028
Cuts at: 6065 1540
Size: 2301
Fragments arranged by size:
2301 1179 1028 794 761 275 248 186
54
BsiHKAI G_wGCw'C
Cuts at: 2263 3003 3339 4887 4972 6043 2263
Size: 740 336 1548 85 1071 3046
Fragments arranged by size:
3046 1548 1071 740 336 85
BsmI GAATG_Cn'
Cuts at: 4337 4430 4337
Size: 93 6733
BsmBI CGTCTCn'nnnn_
Cuts at: 3785 3785
Size: 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2263 2281 2294 2485 2595 3003
Size: 399 225 248 18 13 191 110 408
Cuts at: 3003 3154 3227 3282 3313 3339 3501 3548 3682
Size: 151 73 55 31 26 162 47 134
Cuts at: 3682 3930 4145 4887 4972 6043 1391
Size: 248 215 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 248 248 225
215 191 162 151 134 110 85 73
55 47 31 26 18 13
BspEI T'CCGG_A
Cuts at: 4049 6489 4049
Size: 2440 4386
BspHI T'CATG_A
Cuts at: 4715 6445 4715
Size: 1730 5096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 2767 2779 4181 844
Size: 1826 97 12 1402 3489
Fragments arranged by size:
3489 1826 1402 97 12
BsrBI CCG'CTC
Cuts at: 1394 1823 4713 1394
Size: 429 2890 3507
Fragments arranged by size:
3507 2890 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5321 5495 65
Size: 1183 4073 174 1396
Fragments arranged by size:
4073 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2665 2807 3477 5470 5657 1700
Size: 110 855 142 670 1993 187 2869
Fragments arranged by size:
2869 1993 855 670 187 142 110
BsrGI T'GTAC_A
Cuts at: 96 3456 96
Size: 3360 3466
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6826
BssSI C'ACGA_G
Cuts at: 4886 5898 4886
Size: 1012 5814
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2782 3967 794
Size: 1988 1185 3653
Fragments arranged by size:
3653 1988 1185
BstBI TT'CG_AA
Cuts at: 5630 5630
Size: 6826
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6826
BstYI r'GATC_y
Cuts at: 1972 4156 4523 4908 4925 6377 6463 6475 6821
Size: 2184 367 385 17 1452 86 12 346
Cuts at: 6821 1972
Size: 1977
Fragments arranged by size:
2184 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2148 2548 3399 2148
Size: 400 851 5575
Fragments arranged by size:
5575 851 400
BtgI C'CryG_G
Cuts at: 513 1065 2004 2024 2893 3234 3289 3330 3563
Size: 552 939 20 869 341 55 41 233
Cuts at: 3563 3939 513
Size: 376 3400
Fragments arranged by size:
3400 939 869 552 376 341 233 55
41 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2533 487
Size: 683 56 1307 4780
Fragments arranged by size:
4780 1307 683 56
BtsI GCAGTG_nn'
Cuts at: 2294 2595 2850 3225 4342 5136 5156 2294
Size: 301 255 375 1117 794 20 3964
Fragments arranged by size:
3964 1117 794 375 301 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6826
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6791
DraI TTT'AAA
Cuts at: 4130 4130
Size: 6826
DraIII CAC_nnn'GTG
Cuts at: 3111 3111
Size: 6826
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5833 809
Size: 507 4517 1802
Fragments arranged by size:
4517 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2412 2694
Size: 54 1425 50 275 18 222 360 282
Cuts at: 2694 2890 2913 2940 3348 3427 3479 3636 3936
Size: 196 23 27 408 79 52 157 300
Cuts at: 3936 4273 5162 6791 8
Size: 337 889 1629 43
Fragments arranged by size:
1629 1425 889 408 360 337 300 282
275 222 196 157 79 54 52 50
43 27 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 3479 4273 1537
Size: 275 240 1427 794 4090
Fragments arranged by size:
4090 1427 794 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 3371 4756 1353
Size: 686 1332 1385 3423
Fragments arranged by size:
3423 1385 1332 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2522 3359 3605 5413 5787 5933
Size: 1064 138 933 837 246 1808 374 146
Cuts at: 5933 387
Size: 1280
Fragments arranged by size:
1808 1280 1064 933 837 374 246 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2258 3031 3035 3107 3431 3809
Size: 393 392 234 773 4 72 324 378
Cuts at: 3809 3923 4078 4889 5486 6273 6541 1239
Size: 114 155 811 597 787 268 1524
Fragments arranged by size:
1524 811 787 773 597 393 392 378
324 268 234 155 114 72 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3593 3863 3593
Size: 270 6556
EcoO109I rG'GnC_Cy
Cuts at: 2233 2458 2481 2482 2791 2932 3285 3309 3778
Size: 225 23 1 309 141 353 24 469
Cuts at: 3778 3868 3907 3964 2233
Size: 90 39 57 5095
Fragments arranged by size:
5095 469 353 309 225 141 90 57
39 24 23 1
EcoRI G'AATT_C
Cuts at: 4135 4513 4135
Size: 378 6448
FspI TGC'GCA
Cuts at: 5332 5332
Size: 6826
HaeII r_GCGC'y
Cuts at: 1678 2164 2207 3298 3688 3739 3849 5973 1678
Size: 486 43 1091 390 51 110 2124 2531
Fragments arranged by size:
2531 2124 1091 486 390 110 51 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3871 3903 5467 5499 5541
Size: 32 135 32 2299 32 1564 32 42
Cuts at: 5541 5573 1373
Size: 32 2626
Fragments arranged by size:
2626 2299 1564 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3022 4170 4416 669
Size: 988 281 1084 1148 246 3079
Fragments arranged by size:
3079 1148 1084 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6826
HpaI GTT'AAC
Cuts at: 4416 4416
Size: 6826
KasI G'GCGC_C
Cuts at: 3294 3735 3294
Size: 441 6385
KpnI G_GTAC'C
Cuts at: 4151 4151
Size: 6826
MfeI C'AATT_G
Cuts at: 4425 4425
Size: 6826
MluI A'CGCG_T
Cuts at: 6702 6702
Size: 6826
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 3232 5940 6124 1305
Size: 1536 391 2708 184 2007
Fragments arranged by size:
2708 2007 1536 391 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2696 2892 2942 3350 3638
Size: 54 1425 343 864 196 50 408 288
Cuts at: 3638 10
Size: 3198
Fragments arranged by size:
3198 1425 864 408 343 288 196 54
50
MslI CAynn'nnrTG
Cuts at: 518 1166 3938 4784 5143 5302 518
Size: 648 2772 846 359 159 2042
Fragments arranged by size:
2772 2042 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2173 2431 2574 3346 3892 4000 4014
Size: 420 147 258 143 772 546 108 14
Cuts at: 4014 4242 4919 6067 6312 1606
Size: 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 546 420 258 245
228 147 143 108 14
NaeI GCC'GGC
Cuts at: 3479 3479
Size: 6826
NarI GG'CG_CC
Cuts at: 3295 3736 3295
Size: 441 6385
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5335
Fragments arranged by size:
5335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6826
NgoMIV G'CCGG_C
Cuts at: 3477 3477
Size: 6826
NheI G'CTAG_C
Cuts at: 1052 3970 1052
Size: 2918 3908
NotI GC'GGCC_GC
Cuts at: 4273 4273
Size: 6826
NspI r_CATG'y
Cuts at: 1132 1229 4184 5729 1132
Size: 97 2955 1545 2229
Fragments arranged by size:
2955 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2230 2272 2464 3541 3772 1348
Size: 882 42 192 1077 231 4402
Fragments arranged by size:
4402 1077 882 231 192 42
PciI A'CATG_T
Cuts at: 5725 5725
Size: 6826
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2236 2875 2922 3111 3745 1108
Size: 939 189 639 47 189 634 4189
Fragments arranged by size:
4189 939 639 634 189 189 47
PmeI GTTT'AAAC
Cuts at: 4130 4130
Size: 6826
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 4895 4927 6408 6440 3434
Size: 32 1429 32 1481 32 3820
Fragments arranged by size:
3820 1481 1429 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2791 3285 3778 2791
Size: 494 493 5839
Fragments arranged by size:
5839 494 493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6826
PsiI TTA'TAA
Cuts at: 4396 4396
Size: 6826
PspOMI G'GGCC_C
Cuts at: 2481 3309 3544 2481
Size: 828 235 5763
Fragments arranged by size:
5763 828 235
PstI C_TGCA'G
Cuts at: 830 2790 3597 4178 830
Size: 1960 807 581 3478
Fragments arranged by size:
3478 1960 807 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6826
PvuII CAG'CTG
Cuts at: 2173 3346 3892 2173
Size: 1173 546 5107
Fragments arranged by size:
5107 1173 546
SacII CC_GC'GG
Cuts at: 2027 2027
Size: 6826
SalI G'TCGA_C
Cuts at: 1936 4168 1936
Size: 2232 4594
SanDI GG'GwC_CC
Cuts at: 3285 3778 3285
Size: 493 6333
SapI GCTCTTCn'nnn_
Cuts at: 2039 2039
Size: 6826
SbfI CC_TGCA'GG
Cuts at: 4178 4178
Size: 6826
ScaI AGT'ACT
Cuts at: 3695 5074 6786 3695
Size: 1379 1712 3735
Fragments arranged by size:
3735 1712 1379
SfcI C'TryA_G
Cuts at: 826 1045 2786 3201 3341 3593 3815 4174 5309
Size: 219 1741 415 140 252 222 359 1135
Cuts at: 5309 5990 6181 826
Size: 681 191 1471
Fragments arranged by size:
1741 1471 1135 681 415 359 252 222
219 191 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 2106 2939 2106
Size: 833 5993
SfoI GGC'GCC
Cuts at: 3296 3737 3296
Size: 441 6385
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6826
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6826
SmaI CCC'GGG
Cuts at: 1872 3283 4153 1872
Size: 1411 870 4545
Fragments arranged by size:
4545 1411 870
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4930 5831 6093 820
Size: 197 926 2987 901 262 1553
Fragments arranged by size:
2987 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4617 493
Size: 4124 2702
SpeI A'CTAG_T
Cuts at: 152 2263 152
Size: 2111 4715
SphI G_CATG'C
Cuts at: 1132 4184 1132
Size: 3052 3774
SspI AAT'ATT
Cuts at: 5 52 4750 5
Size: 47 4698 2081
Fragments arranged by size:
4698 2081 47
StuI AGG'CCT
Cuts at: 1517 2100 1517
Size: 583 6243
StyI C'CwwG_G
Cuts at: 513 1065 2004 2928 4265 513
Size: 552 939 924 1337 3074
Fragments arranged by size:
3074 1337 939 924 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2246 3107 3733 4853 4870 5023 5208
Size: 1255 666 861 626 1120 17 153 185
Cuts at: 5208 5653 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1120 861 666 626 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3456 3693 5072
Size: 274 80 33 51 1443 1479 237 1379
Cuts at: 5072 6784 96
Size: 1712 138
Fragments arranged by size:
1712 1479 1443 1379 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3181 3213 6786 6818 3181
Size: 32 3573 32 3189
Fragments arranged by size:
3573 3189 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6826
XbaI T'CTAG_A
Cuts at: 4162 4162
Size: 6826
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2857 2935 3112 3320 3516 3856 2857
Size: 78 177 208 196 340 5827
Fragments arranged by size:
5827 340 208 196 177 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6826
XmaI C'CCGG_G
Cuts at: 1870 3281 4151 1870
Size: 1411 870 4545
Fragments arranged by size:
4545 1411 870
XmnI GAAnn'nnTTC
Cuts at: 4955 4955
Size: 6826
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4634 276
Size: 53 83 186 936 3100 2468
Fragments arranged by size:
3100 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I
EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SexAI SrfI
SwaI