Restriction map for
pFN21AE0343
(Circular) (Six-base) MAPSORT of: pFN21AE0343.seq Check: 6551 from: 1 to: 6520
pFN21AE0343 6520 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2202 2238 3863 1937
Size: 265 36 1625 4594
Fragments arranged by size:
4594 1625 265 36
Acc65I G'GTAC_C
Cuts at: 3099 3841 3099
Size: 742 5778
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4583 5967 6235 1632
Size: 2951 1384 268 1917
Fragments arranged by size:
2951 1917 1384 268
AfeI AGC'GCT
Cuts at: 3782 3782
Size: 6520
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6323
AflIII A'CryG_T
Cuts at: 1203 2550 3190 5419 6396 1203
Size: 1347 640 2229 977 1327
Fragments arranged by size:
2229 1347 1327 977 640
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 3742 5249 3742
Size: 1507 5013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2883 2886 1184
Size: 1699 3 4818
Fragments arranged by size:
4818 1699 3
AlwNI CAG_nnn'CTG
Cuts at: 2921 2962 3253 3495 3517 4263 5835 2921
Size: 41 291 242 22 746 1572 3606
Fragments arranged by size:
3606 1572 746 291 242 41 22
ApaI G_GGCC'C
Cuts at: 2914 2914
Size: 6520
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 3829 4055 4207 6316 1493
Size: 2336 226 152 2109 1697
Fragments arranged by size:
2336 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 3344 3716 3845 1870
Size: 73 1401 372 129 4545
Fragments arranged by size:
4545 1401 372 129 73
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3099 3841 5296 618
Size: 325 217 627 1312 742 1455 1842
Fragments arranged by size:
1842 1455 1312 742 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2192 2702 2841 2858 2914 3345 3435 3839
Size: 801 510 139 17 56 431 90 404
Cuts at: 3839 1391
Size: 4072
Fragments arranged by size:
4072 801 510 431 404 139 90 56
17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3300 928
Size: 971 1401 4148
Fragments arranged by size:
4148 1401 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 2410 1548
Size: 862 5658
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2462 2825 5131
Size: 107 122 71 631 605 790 363 2306
Cuts at: 5131 136
Size: 1525
Fragments arranged by size:
2306 1525 790 631 605 363 122 107
71
BglII A'GATC_T
Cuts at: 2121 6515 2121
Size: 4394 2126
BlpI GC'TnA_GC
Cuts at: 3243 3476 3937 3243
Size: 233 461 5826
Fragments arranged by size:
5826 461 233
Bme1580I G_kGCm'C
Cuts at: 1790 2914 3024 3546 4581 5737 1790
Size: 1124 110 522 1035 1156 2573
Fragments arranged by size:
2573 1156 1124 1035 522 110
BmgBI CAC'GTC
Cuts at: 1318 2014 2080 1318
Size: 696 66 5758
Fragments arranged by size:
5758 696 66
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2077 2415 3505
Size: 416 53 83 399 24 660 338 1090
Cuts at: 3505 4251 5209 442
Size: 746 958 1753
Fragments arranged by size:
1753 1090 958 746 660 416 399 338
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2727 2855 2928 3299 3506 3743 5180
Size: 887 601 128 73 371 207 237 1437
Cuts at: 5180 1239
Size: 2579
Fragments arranged by size:
2579 1437 887 601 371 237 207 128
73
Bpu10I CC'TnA_GC
Cuts at: 3398 3398
Size: 6520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 4645 5510 5808 2387
Size: 2258 865 298 3099
Fragments arranged by size:
3099 2258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3583 5183 882
Size: 617 2084 1600 2219
Fragments arranged by size:
2219 2084 1600 617
BsaAI yAC'GTr
Cuts at: 493 1204 2553 3193 4311 493
Size: 711 1349 640 1118 2702
Fragments arranged by size:
2702 1349 1118 711 640
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2417 4327 4709 5344
Size: 53 83 186 936 884 1910 382 635
Cuts at: 5344 275
Size: 1451
Fragments arranged by size:
1910 1451 936 884 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3216 4953 5351 5625 5772 6183 3216
Size: 1737 398 274 147 411 3553
Fragments arranged by size:
3553 1737 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3164 3194 1178
Size: 30 165 30 1761 30 4504
Fragments arranged by size:
4504 1761 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2681 2804 3050 3244 3281 3344 3422
Size: 937 558 123 246 194 37 63 78
Cuts at: 3422 1186
Size: 4284
Fragments arranged by size:
4284 937 558 246 194 123 78 63
37
BseYI C'CCAG_C
Cuts at: 2088 2092 2707 3591 3708 3813 5723 6219 2088
Size: 4 615 884 117 105 1910 496 2389
Fragments arranged by size:
2389 1910 884 615 496 117 105 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 2713 3530 1163
Size: 573 579 398 817 4153
Fragments arranged by size:
4153 817 579 573 398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 4581 4666 5737 3435
Size: 1146 85 1071 4218
Fragments arranged by size:
4218 1146 1071 85
BsmI GAATG_Cn'
Cuts at: 4031 4124 4031
Size: 93 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2192 2702 2841 2858 2914 3024 3345
Size: 399 402 510 139 17 56 110 321
Cuts at: 3345 3435 3546 3839 4581 4666 5737 1391
Size: 90 111 293 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 402 399 321 293
139 111 110 90 85 56 17
BspEI T'CCGG_A
Cuts at: 3216 6183 3216
Size: 2967 3553
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3875 844
Size: 3031 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2831 2902 4407 1394
Size: 429 1008 71 1505 3507
Fragments arranged by size:
3507 1505 1008 429 71
BsrDI GCAATG_nn'
Cuts at: 65 1248 5015 5189 65
Size: 1183 3767 174 1396
Fragments arranged by size:
3767 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3637 5164 5351 1700
Size: 110 1827 1527 187 2869
Fragments arranged by size:
2869 1827 1527 187 110
BsrGI T'GTAC_A
Cuts at: 96 2499 96
Size: 2403 4117
BssHII G'CGCG_C
Cuts at: 1925 2021 1925
Size: 96 6424
BssSI C'ACGA_G
Cuts at: 4580 5592 4580
Size: 1012 5508
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3122 3253 3517 3541 794
Size: 2328 131 264 24 3773
Fragments arranged by size:
3773 2328 264 131 24
BstBI TT'CG_AA
Cuts at: 3418 5324 3418
Size: 1906 4614
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2952 3563 2458
Size: 494 611 5415
Fragments arranged by size:
5415 611 494
BstYI r'GATC_y
Cuts at: 1972 2121 2398 3850 4217 4602 4619 6071 6157
Size: 149 277 1452 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1452 385 367 346 277 149
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2808 3327 2808
Size: 519 6001
BtgI C'CryG_G
Cuts at: 513 1065 2004 2451 2607 3732 3807 513
Size: 552 939 447 156 1125 75 3226
Fragments arranged by size:
3226 1125 939 552 447 156 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3424 487
Size: 683 56 2198 3583
Fragments arranged by size:
3583 2198 683 56
BtsI GCAGTG_nn'
Cuts at: 3254 3390 4036 4830 4850 3254
Size: 136 646 794 20 4924
Fragments arranged by size:
4924 794 646 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6485
DraI TTT'AAA
Cuts at: 3824 3824
Size: 6520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3173 3575 5527 809
Size: 507 1857 402 1952 1802
Fragments arranged by size:
1952 1857 1802 507 402
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2523 3631 3681
Size: 54 1425 50 275 18 693 1108 50
Cuts at: 3681 3729 3967 4856 6485 8
Size: 48 238 889 1629 43
Fragments arranged by size:
1629 1425 1108 889 693 275 238 54
50 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2293 3440 4450 1353
Size: 940 1147 1010 3423
Fragments arranged by size:
3423 1147 1010 940
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3433 3433
Size: 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2727 2855 2928 3299 3506 3743
Size: 393 494 601 128 73 371 207 237
Cuts at: 3743 4583 5180 5967 6235 1239
Size: 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 601 597 494 393 371
268 237 207 128 73
EcoO109I rG'GnC_Cy
Cuts at: 2245 2332 2428 2518 2574 2640 2735 2910 2911
Size: 87 96 90 56 66 95 175 1
Cuts at: 2911 3175 2245
Size: 264 5590
Fragments arranged by size:
5590 264 175 96 95 90 87 66
56 1
EcoRI G'AATT_C
Cuts at: 3829 4207 3829
Size: 378 6142
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 3072 3784 5667 1678
Size: 1394 712 1883 2531
Fragments arranged by size:
2531 1883 1394 712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2345 2377 2585
Size: 32 135 32 671 32 70 32 208
Cuts at: 2585 2617 2909 2941 3728 3760 5161 5193 5235
Size: 32 292 32 787 32 1401 32 42
Cuts at: 5235 5267 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 787 671 292 208 135 70
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3864 4110 669
Size: 988 281 1926 246 3079
Fragments arranged by size:
3079 1926 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3103 3845 3103
Size: 742 5778
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 3186 5634 5818 1305
Size: 1260 621 2448 184 2007
Fragments arranged by size:
2448 2007 1260 621 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 3633 3683 10
Size: 54 1425 343 693 1108 50 2847
Fragments arranged by size:
2847 1425 1108 693 343 54 50
MslI CAynn'nnrTG
Cuts at: 518 1166 3561 4478 4837 4996 518
Size: 648 2395 917 359 159 2042
Fragments arranged by size:
2395 2042 917 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2618 3371 3574 3679 3936 4613 5761
Size: 486 526 753 203 105 257 677 1148
Cuts at: 5761 6006 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 753 677 526 486 257 245
203 105
NaeI GCC'GGC
Cuts at: 3639 3639
Size: 6520
NcoI C'CATG_G
Cuts at: 513 1065 2004 2451 2607 3807 513
Size: 552 939 447 156 1200 3226
Fragments arranged by size:
3226 1200 939 552 447 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6520
NgoMIV G'CCGG_C
Cuts at: 3637 3637
Size: 6520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 2602 2893 3542 3878 5423 1132
Size: 97 1373 291 649 336 1545 2229
Fragments arranged by size:
2229 1545 1373 649 336 291 97
PasI CC'CwG_GG
Cuts at: 1348 2761 3360 1348
Size: 1413 599 4508
Fragments arranged by size:
4508 1413 599
PciI A'CATG_T
Cuts at: 5419 5419
Size: 6520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2067 1108
Size: 959 5561
PfoI T'CCnGG_A
Cuts at: 2384 2849 3462 3522 2384
Size: 465 613 60 5382
Fragments arranged by size:
5382 613 465 60
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PmlI CAC'GTG
Cuts at: 2553 3193 2553
Size: 640 5880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2339 2371 4589 4621 6102 6134 2339
Size: 32 2218 32 1481 32 2725
Fragments arranged by size:
2725 2218 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2332 2428 2518 2640 3175 2332
Size: 96 90 122 535 5677
Fragments arranged by size:
5677 535 122 96 90
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 4090 4090
Size: 6520
PspOMI G'GGCC_C
Cuts at: 2910 2910
Size: 6520
PstI C_TGCA'G
Cuts at: 830 3084 3228 3265 3678 3872 830
Size: 2254 144 37 413 194 3478
Fragments arranged by size:
3478 2254 413 194 144 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
PvuII CAG'CTG
Cuts at: 2092 3574 3679 2092
Size: 1482 105 4933
Fragments arranged by size:
4933 1482 105
SacI G_AGCT'C
Cuts at: 3435 3435
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SanDI GG'GwC_CC
Cuts at: 2332 2640 2332
Size: 308 6212
SapI GCTCTTCn'nnn_
Cuts at: 3440 3440
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SfcI C'TryA_G
Cuts at: 826 1045 2203 3080 3224 3261 3674 3868 5003
Size: 219 1158 877 144 37 413 194 1135
Cuts at: 5003 5684 5875 826
Size: 681 191 1471
Fragments arranged by size:
1471 1158 1135 877 681 413 219 194
191 144 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 2462 2825 2462
Size: 363 6157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3718 3847 1872
Size: 1846 129 4545
Fragments arranged by size:
4545 1846 129
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 4624 5525 5787 820
Size: 197 926 459 2222 901 262 1553
Fragments arranged by size:
2222 1553 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SrfI GCCC'GGGC
Cuts at: 3718 3718
Size: 6520
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 2813 3010 1517
Size: 1296 197 5027
Fragments arranged by size:
5027 1296 197
StyI C'CwwG_G
Cuts at: 513 1065 2004 2451 2607 2823 2841 2979 3807
Size: 552 939 447 156 216 18 138 828
Cuts at: 3807 3959 513
Size: 152 3074
Fragments arranged by size:
3074 939 828 552 447 216 156 152
138 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2753 4547 4564 4717 4902 5347 325
Size: 1255 1173 1794 17 153 185 445 1498
Fragments arranged by size:
1794 1498 1255 1173 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2499 3180 3333
Size: 274 80 33 51 1443 522 681 153
Cuts at: 3333 4766 6478 96
Size: 1433 1712 138
Fragments arranged by size:
1712 1443 1433 681 522 274 153 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2542 2574 6480 6512 2542
Size: 32 3906 32 2550
Fragments arranged by size:
3906 2550 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3319 3856 3319
Size: 537 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2458 2830 2011
Size: 447 372 5701
Fragments arranged by size:
5701 447 372
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3716 3845 1870
Size: 1846 129 4545
Fragments arranged by size:
4545 1846 129
XmnI GAAnn'nnTTC
Cuts at: 2354 2537 4649 2354
Size: 183 2112 4225
Fragments arranged by size:
4225 2112 183
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspXI
PsrI RsrII SacII SexAI SfoI SwaI