Restriction map for
pFN21AE0341
(Circular) (Six-base) MAPSORT of: pFN21AE0341.seq Check: 2813 from: 1 to: 6757
pFN21AE0341 6757 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3534 4567 278
Size: 53 83 186 936 1998 1033 2468
Fragments arranged by size:
2468 1998 1033 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4100 1937
Size: 2163 4594
Acc65I G'GTAC_C
Cuts at: 3858 4078 3858
Size: 220 6537
AclI AA'CG_TT
Cuts at: 1563 4884 5257 1563
Size: 3321 373 3063
Fragments arranged by size:
3321 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2320 2891 4820 6204 6472 1632
Size: 688 571 1929 1384 268 1917
Fragments arranged by size:
1929 1917 1384 688 571 268
AfeI AGC'GCT
Cuts at: 2076 2076
Size: 6757
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6560
AflIII A'CryG_T
Cuts at: 1203 5656 6633 1203
Size: 4453 977 1327
Fragments arranged by size:
4453 1327 977
AgeI A'CCGG_T
Cuts at: 3546 5588 3546
Size: 2042 4715
AhdI GACnn_n'nnGTC
Cuts at: 5486 5486
Size: 6757
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3820 3941 1184
Size: 2636 121 4000
Fragments arranged by size:
4000 2636 121
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 6725
AlwNI CAG_nnn'CTG
Cuts at: 3433 4500 6072 3433
Size: 1067 1572 4118
Fragments arranged by size:
4118 1572 1067
ApaI G_GGCC'C
Cuts at: 2124 2191 2124
Size: 67 6690
ApaLI G'TGCA_C
Cuts at: 4814 5970 4814
Size: 1156 5601
ApoI r'AATT_y
Cuts at: 1493 2906 3388 3508 4066 4292 4444 6553 1493
Size: 1413 482 120 558 226 152 2109 1697
Fragments arranged by size:
2109 1697 1413 558 482 226 152 120
AseI AT'TA_AT
Cuts at: 160 1032 5311 160
Size: 872 4279 1606
Fragments arranged by size:
4279 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2739 2840 3070 4082 1870
Size: 73 98 698 101 230 1012 4545
Fragments arranged by size:
4545 1012 698 230 101 98 73
BamHI G'GATC_C
Cuts at: 4087 4454 4087
Size: 367 6390
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2226 3858 3951 4078
Size: 325 217 627 296 143 1632 93 127
Cuts at: 4078 5533 618
Size: 1455 1842
Fragments arranged by size:
1842 1632 1455 627 325 296 217 143
127 93
BanII G_rGCy'C
Cuts at: 1391 2124 2191 2418 3285 3342 3480 4056 4076
Size: 733 67 227 867 57 138 576 20
Cuts at: 4076 1391
Size: 4072
Fragments arranged by size:
4072 867 733 576 227 138 67 57
20
BbeI G_GCGC'C
Cuts at: 3955 3955
Size: 6757
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3526 3705 928
Size: 971 1627 179 3980
Fragments arranged by size:
3980 1627 971 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4948 4982 1903
Size: 34 3011 34 3678
Fragments arranged by size:
3678 3011 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2800 4649 5859 2800
Size: 1849 1210 3698
Fragments arranged by size:
3698 1849 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6757
BfrBI ATG'CAT
Cuts at: 3080 3080
Size: 6757
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5368 136
Size: 107 122 71 631 605 3696 1525
Fragments arranged by size:
3696 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6752 6752
Size: 6757
BlpI GC'TnA_GC
Cuts at: 2312 2729 4174 2312
Size: 417 1445 4895
Fragments arranged by size:
4895 1445 417
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2124 2191 2997 3561 3735 4818 5974
Size: 298 36 67 806 564 174 1083 1156
Cuts at: 5974 1790
Size: 2573
Fragments arranged by size:
2573 1156 1083 806 564 298 174 67
36
BmgBI CAC'GTC
Cuts at: 1318 2449 1318
Size: 1131 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4488 5446 442
Size: 416 53 83 399 24 3071 958 1753
Fragments arranged by size:
3071 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3114 1056
Size: 2058 4699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3263 3431 3464 3779 3977 3996 5417 1239
Size: 2024 168 33 315 198 19 1421 2579
Fragments arranged by size:
2579 2024 1421 315 198 168 33 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 4882 5747 6045 2804
Size: 2078 865 298 3516
Fragments arranged by size:
3516 2078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2769 5420 882
Size: 617 1270 2651 2219
Fragments arranged by size:
2651 2219 1270 617
BsaAI yAC'GTr
Cuts at: 493 1204 4548 493
Size: 711 3344 2702
Fragments arranged by size:
3344 2702 711
BsaBI GATnn'nnATC
Cuts at: 6751 6751
Size: 6757
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3531 3952 4564
Size: 53 83 186 936 834 1164 421 612
Cuts at: 4564 4946 5581 275
Size: 382 635 1451
Fragments arranged by size:
1451 1164 936 834 635 612 421 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3177 3546 5190 5588 5862 6009 6420 3177
Size: 369 1644 398 274 147 411 3514
Fragments arranged by size:
3514 1644 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3626 3656 1178
Size: 30 165 30 2223 30 4279
Fragments arranged by size:
4279 2223 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3428 4064 1186
Size: 2242 636 3879
Fragments arranged by size:
3879 2242 636
BseYI C'CCAG_C
Cuts at: 2071 2110 2117 2410 3188 3438 5960 6456 2071
Size: 39 7 293 778 250 2522 496 2372
Fragments arranged by size:
2522 2372 778 496 293 250 39 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2051 2548 3636 1163
Size: 573 315 497 1088 4284
Fragments arranged by size:
4284 1088 573 497 315
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3729 4207 4968 5996 1540
Size: 275 186 1728 478 761 1028 2301
Fragments arranged by size:
2301 1728 1028 761 478 275 186
BsiHKAI G_wGCw'C
Cuts at: 3249 3285 4056 4818 4903 5974 3249
Size: 36 771 762 85 1071 4032
Fragments arranged by size:
4032 1071 771 762 85 36
BsmI GAATG_Cn'
Cuts at: 3080 4268 4361 3080
Size: 1188 93 5476
Fragments arranged by size:
5476 1188 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2124 2191 2418 2997 3249 3285
Size: 399 298 36 67 227 579 252 36
Cuts at: 3285 3342 3480 3561 3735 4056 4076 4818 4903
Size: 57 138 81 174 321 20 742 85
Cuts at: 4903 5974 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 321 298 252
227 174 138 85 81 67 57 36
36 20
BspEI T'CCGG_A
Cuts at: 6420 6420
Size: 6757
BspHI T'CATG_A
Cuts at: 4646 6376 4646
Size: 1730 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4112 844
Size: 3268 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2185 2736 4644 1394
Size: 429 362 551 1908 3507
Fragments arranged by size:
3507 1908 551 429 362
BsrDI GCAATG_nn'
Cuts at: 65 1248 5252 5426 65
Size: 1183 4004 174 1396
Fragments arranged by size:
4004 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3234 3546 5401 5588 1700
Size: 110 1424 312 1855 187 2869
Fragments arranged by size:
2869 1855 1424 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6757
BssHII G'CGCG_C
Cuts at: 1925 2155 1925
Size: 230 6527
BssSI C'ACGA_G
Cuts at: 3878 4817 5829 3878
Size: 939 1012 4806
Fragments arranged by size:
4806 1012 939
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6757
BstBI TT'CG_AA
Cuts at: 5561 5561
Size: 6757
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6757
BstXI CCAn_nnnn'nTGG
Cuts at: 2771 3977 2771
Size: 1206 5551
BstYI r'GATC_y
Cuts at: 1972 2857 4087 4454 4839 4856 6308 6394 6406
Size: 885 1230 367 385 17 1452 86 12
Cuts at: 6406 6752 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1230 885 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2628 3863 2628
Size: 1235 5522
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2192 2222 2619 3202 3393
Size: 552 939 120 68 30 397 583 191
Cuts at: 3393 3675 513
Size: 282 3595
Fragments arranged by size:
3595 939 583 552 397 282 191 120
68 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 2425 487
Size: 683 56 818 381 4819
Fragments arranged by size:
4819 818 683 381 56
BtsI GCAGTG_nn'
Cuts at: 2181 4273 5067 5087 2181
Size: 2092 794 20 3851
Fragments arranged by size:
3851 2092 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3382 3417 529
Size: 35 2818 35 3869
Fragments arranged by size:
3869 2818 35 35
DraI TTT'AAA
Cuts at: 4061 4061
Size: 6757
DraIII CAC_nnn'GTG
Cuts at: 3169 3694 3910 3169
Size: 525 216 6016
Fragments arranged by size:
6016 525 216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3530 5764 809
Size: 507 2214 2234 1802
Fragments arranged by size:
2234 2214 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2482 2862
Size: 54 1425 50 275 18 225 427 380
Cuts at: 2862 3363 3678 4204 5093 6722 8
Size: 501 315 526 889 1629 43
Fragments arranged by size:
1629 1425 889 526 501 427 380 315
275 225 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4204 1537
Size: 275 2392 4090
Fragments arranged by size:
4090 2392 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2339 3485 4687 1353
Size: 986 1146 1202 3423
Fragments arranged by size:
3423 1202 1146 986
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2279 3635 5344 5718 5864 387
Size: 1064 138 690 1356 1709 374 146 1280
Fragments arranged by size:
1709 1356 1280 1064 690 374 146 138
EcoICRI GAG'CTC
Cuts at: 3283 4054 3283
Size: 771 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2320 2891 3263 3431 3464 3779 3977
Size: 393 688 571 372 168 33 315 198
Cuts at: 3977 3996 4820 5417 6204 6472 1239
Size: 19 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 688 597 571 393 372
315 268 198 168 33 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2960 3380 2960
Size: 420 6337
EcoO109I rG'GnC_Cy
Cuts at: 2036 2188 4016 2036
Size: 152 1828 4777
Fragments arranged by size:
4777 1828 152
EcoRI G'AATT_C
Cuts at: 3388 4066 4444 3388
Size: 678 378 5701
Fragments arranged by size:
5701 678 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 6757
FspI TGC'GCA
Cuts at: 5263 5263
Size: 6757
HaeII r_GCGC'y
Cuts at: 1678 2078 2118 3754 3955 5904 1678
Size: 400 40 1636 201 1949 2531
Fragments arranged by size:
2531 1949 1636 400 201 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2288 2320 2600 2632 2647
Size: 32 135 32 716 32 280 32 15
Cuts at: 2647 2679 3533 3565 5398 5430 5472 5504 1373
Size: 32 854 32 1833 32 42 32 2626
Fragments arranged by size:
2626 1833 854 716 280 135 42 32
32 32 32 32 32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 4101 4347 669
Size: 988 281 637 1526 246 3079
Fragments arranged by size:
3079 1526 988 637 281 246
HindIII A'AGCT_T
Cuts at: 748 3787 748
Size: 3039 3718
HpaI GTT'AAC
Cuts at: 4347 4347
Size: 6757
KasI G'GCGC_C
Cuts at: 3951 3951
Size: 6757
KpnI G_GTAC'C
Cuts at: 3862 4082 3862
Size: 220 6537
MfeI C'AATT_G
Cuts at: 4356 4356
Size: 6757
MluI A'CGCG_T
Cuts at: 6633 6633
Size: 6757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2752 5871 6055 1305
Size: 1447 3119 184 2007
Fragments arranged by size:
3119 2007 1447 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2484 2864 10
Size: 54 1425 343 225 427 380 3903
Fragments arranged by size:
3903 1425 427 380 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2990 3428 3491 4715 5074 5233 518
Size: 648 1824 438 63 1224 359 159 2042
Fragments arranged by size:
2042 1824 1224 648 438 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2018 2126 2194 2224 3015 3100 3298 3442
Size: 412 108 68 30 791 85 198 144
Cuts at: 3442 4173 4850 5998 6243 1606
Size: 731 677 1148 245 2120
Fragments arranged by size:
2120 1148 791 731 677 412 245 198
144 108 85 68 30
NarI GG'CG_CC
Cuts at: 3952 3952
Size: 6757
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5266
Fragments arranged by size:
5266 939 552
NdeI CA'TA_TG
Cuts at: 387 2724 387
Size: 2337 4420
NheI G'CTAG_C
Cuts at: 1052 3110 1052
Size: 2058 4699
NotI GC'GGCC_GC
Cuts at: 4204 4204
Size: 6757
NsiI A_TGCA'T
Cuts at: 3082 3082
Size: 6757
NspI r_CATG'y
Cuts at: 1132 1229 4115 5660 1132
Size: 97 2886 1545 2229
Fragments arranged by size:
2886 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6757
PciI A'CATG_T
Cuts at: 5656 5656
Size: 6757
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3169 1108
Size: 2061 4696
PfoI T'CCnGG_A
Cuts at: 2913 3240 3756 2913
Size: 327 516 5914
Fragments arranged by size:
5914 516 327
PmeI GTTT'AAAC
Cuts at: 4061 4061
Size: 6757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 4826 4858 6339 6371 3626
Size: 32 1168 32 1481 32 4012
Fragments arranged by size:
4012 1481 1168 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2036 2036
Size: 6757
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6757
PsiI TTA'TAA
Cuts at: 4327 4327
Size: 6757
PspOMI G'GGCC_C
Cuts at: 2120 2187 2120
Size: 67 6690
PspXI vC'TCGA_Gb
Cuts at: 2840 2840
Size: 6757
PstI C_TGCA'G
Cuts at: 830 2136 2154 4109 830
Size: 1306 18 1955 3478
Fragments arranged by size:
3478 1955 1306 18
PvuI CG_AT'CG
Cuts at: 2001 3729 2001
Size: 1728 5029
PvuII CAG'CTG
Cuts at: 3298 3442 3298
Size: 144 6613
SacI G_AGCT'C
Cuts at: 3285 4056 3285
Size: 771 5986
SacII CC_GC'GG
Cuts at: 2127 2195 2225 2127
Size: 68 30 6659
Fragments arranged by size:
6659 68 30
SalI G'TCGA_C
Cuts at: 1936 4099 1936
Size: 2163 4594
SanDI GG'GwC_CC
Cuts at: 2036 2036
Size: 6757
SapI GCTCTTCn'nnn_
Cuts at: 2339 3485 2339
Size: 1146 5611
SbfI CC_TGCA'GG
Cuts at: 4109 4109
Size: 6757
ScaI AGT'ACT
Cuts at: 5005 6717 5005
Size: 1712 5045
SexAI A'CCwGG_T
Cuts at: 2568 2568
Size: 6757
SfcI C'TryA_G
Cuts at: 826 1045 2132 2150 3610 4105 5240 5921 6112
Size: 219 1087 18 1460 495 1135 681 191
Cuts at: 6112 826
Size: 1471
Fragments arranged by size:
1471 1460 1135 1087 681 495 219 191
18
SfoI GGC'GCC
Cuts at: 3953 3953
Size: 6757
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6757
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6757
SmaI CCC'GGG
Cuts at: 1872 2043 2741 4084 1872
Size: 171 698 1343 4545
Fragments arranged by size:
4545 1343 698 171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 2840 4861 5762 6024 820
Size: 197 926 876 21 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 876 262 197 21
SnaBI TAC'GTA
Cuts at: 493 4548 493
Size: 4055 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6757
SphI G_CATG'C
Cuts at: 1132 4115 1132
Size: 2983 3774
SspI AAT'ATT
Cuts at: 5 52 4681 5
Size: 47 4629 2081
Fragments arranged by size:
4629 2081 47
StuI AGG'CCT
Cuts at: 1517 2474 1517
Size: 957 5800
StyI C'CwwG_G
Cuts at: 513 1065 2004 4196 513
Size: 552 939 2192 3074
Fragments arranged by size:
3074 2192 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 2381 2481 2693 2777 4784 4801
Size: 1255 505 296 100 212 84 2007 17
Cuts at: 4801 4954 5139 5584 325
Size: 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 505 445 296 212 185
153 100 84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 5003 6715
Size: 274 80 33 51 1443 450 2576 1712
Cuts at: 6715 96
Size: 138
Fragments arranged by size:
2576 1712 1443 450 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6717 6749 6717
Size: 32 6725
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6757
XbaI T'CTAG_A
Cuts at: 4093 4093
Size: 6757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3166 3430 3166
Size: 264 6493
XhoI C'TCGA_G
Cuts at: 1943 2840 1943
Size: 897 5860
XmaI C'CCGG_G
Cuts at: 1870 2041 2739 4082 1870
Size: 171 698 1343 4545
Fragments arranged by size:
4545 1343 698 171
XmnI GAAnn'nnTTC
Cuts at: 2909 4886 2909
Size: 1977 4780
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3532 4565 276
Size: 53 83 186 936 1998 1033 2468
Fragments arranged by size:
2468 1998 1033 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI
BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI
PacI PmlI PsrI RsrII SfiI SrfI SwaI