Restriction map for
pFN21AE0340
(Circular) (Six-base) MAPSORT of: pFN21AE0340.seq Check: 1937 from: 1 to: 6226
pFN21AE0340 6226 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 278
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2541 3569 1937
Size: 604 1028 4594
Fragments arranged by size:
4594 1028 604
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2396 4289 5673 5941 1632
Size: 455 309 1893 1384 268 1917
Fragments arranged by size:
1917 1893 1384 455 309 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 3035 5125 6102 1203
Size: 1832 2090 977 1327
Fragments arranged by size:
2090 1832 1327 977
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 3040 1166
Size: 1874 4352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6226
AlwNI CAG_nnn'CTG
Cuts at: 2668 2719 3969 5541 2668
Size: 51 1250 1572 3353
Fragments arranged by size:
3353 1572 1250 51
ApaLI G'TGCA_C
Cuts at: 4283 5439 4283
Size: 1156 5070
ApoI r'AATT_y
Cuts at: 1493 2564 2864 3535 3761 3913 6022 1493
Size: 1071 300 671 226 152 2109 1697
Fragments arranged by size:
2109 1697 1071 671 300 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 3551 1870
Size: 73 1338 270 4545
Fragments arranged by size:
4545 1338 270 73
BamHI G'GATC_C
Cuts at: 3556 3923 3556
Size: 367 5859
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3547 5002 618
Size: 325 217 627 1760 1455 1842
Fragments arranged by size:
1842 1760 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2881 3360 3545 1391
Size: 1490 479 185 4072
Fragments arranged by size:
4072 1490 479 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3003 928
Size: 971 1104 4151
Fragments arranged by size:
4151 1104 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4417 4451 1903
Size: 34 50 34 2396 34 3678
Fragments arranged by size:
3678 2396 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4118 5328 4118
Size: 1210 5016
BclI T'GATC_A
Cuts at: 1548 2739 1548
Size: 1191 5035
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2424 3239 4837
Size: 107 122 71 631 605 752 815 1598
Cuts at: 4837 136
Size: 1525
Fragments arranged by size:
1598 1525 815 752 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 3643 3643
Size: 6226
Bme1580I G_kGCm'C
Cuts at: 1790 4287 5443 1790
Size: 2497 1156 2573
Fragments arranged by size:
2573 2497 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2472 3957 4915
Size: 416 53 83 399 24 1055 1485 958
Cuts at: 4915 442
Size: 1753
Fragments arranged by size:
1753 1485 1055 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2556 3288 4886 1239
Size: 942 375 732 1598 2579
Fragments arranged by size:
2579 1598 942 732 375
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2250 4351 5216 5514 2250
Size: 2101 865 298 2962
Fragments arranged by size:
2962 2101 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4889 882
Size: 617 3390 2219
Fragments arranged by size:
3390 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3038 3042 4017 493
Size: 711 1834 4 975 2702
Fragments arranged by size:
2702 1834 975 711 4
BsaBI GATnn'nnATC
Cuts at: 3344 6220 3344
Size: 2876 3350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4033 4415 5050 275
Size: 53 83 186 936 2500 382 635 1451
Fragments arranged by size:
2500 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4659 5057 5331 5478 5889 4659
Size: 398 274 147 411 4996
Fragments arranged by size:
4996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2715 2745 1178
Size: 30 165 30 1312 30 4659
Fragments arranged by size:
4659 1312 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 1186
Size: 979 5247
BseYI C'CCAG_C
Cuts at: 2150 2777 5429 5925 2150
Size: 627 2652 496 2451
Fragments arranged by size:
2652 2451 627 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5653
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3676 4437 5465 1540
Size: 275 186 1675 761 1028 2301
Fragments arranged by size:
2301 1675 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 4287 4372 5443 2304
Size: 1983 85 1071 3087
Fragments arranged by size:
3087 1983 1071 85
BsiWI C'GTAC_G
Cuts at: 3038 3038
Size: 6226
BsmI GAATG_Cn'
Cuts at: 2400 2772 2876 3737 3830 2400
Size: 372 104 861 93 4796
Fragments arranged by size:
4796 861 372 104 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2591 2591
Size: 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2881 3360 3545 4287 4372 5443
Size: 399 514 577 479 185 742 85 1071
Cuts at: 5443 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 577 514 479 399 185
85
BspEI T'CCGG_A
Cuts at: 5889 5889
Size: 6226
BspHI T'CATG_A
Cuts at: 3207 4115 5845 3207
Size: 908 1730 3588
Fragments arranged by size:
3588 1730 908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3048 3581 844
Size: 2204 533 3489
Fragments arranged by size:
3489 2204 533
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4721 4895 65
Size: 1183 3473 174 1396
Fragments arranged by size:
3473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4870 5057 1700
Size: 110 3060 187 2869
Fragments arranged by size:
3060 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6226
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 2880 4286 5298 2880
Size: 1406 1012 3808
Fragments arranged by size:
3808 1406 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6226
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6226
BstYI r'GATC_y
Cuts at: 1972 2473 3102 3556 3923 4308 4325 5777 5863
Size: 501 629 454 367 385 17 1452 86
Cuts at: 5863 5875 6221 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 629 501 454 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3072 3072
Size: 6226
BtgI C'CryG_G
Cuts at: 513 1065 2056 513
Size: 552 991 4683
Fragments arranged by size:
4683 991 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5487
Fragments arranged by size:
5487 683 56
BtsI GCAGTG_nn'
Cuts at: 3742 4536 4556 3742
Size: 794 20 5412
Fragments arranged by size:
5412 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6191
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DraIII CAC_nnn'GTG
Cuts at: 2950 2950
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5233 809
Size: 507 3917 1802
Fragments arranged by size:
3917 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3044 3271 3673
Size: 54 1425 50 275 18 1214 227 402
Cuts at: 3673 4562 6191 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1214 889 402 275 227 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3673 1537
Size: 275 1861 4090
Fragments arranged by size:
4090 1861 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4156 1353
Size: 2803 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4813 5187 5333 387
Size: 1064 138 3224 374 146 1280
Fragments arranged by size:
3224 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2181 2396 2556 3288 4289 4886
Size: 393 455 94 215 160 732 1001 597
Cuts at: 4886 5673 5941 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1001 787 732 597 455 393 268
215 160 94
EcoNI CCTnn'n_nnAGG
Cuts at: 2672 2672
Size: 6226
EcoO109I rG'GnC_Cy
Cuts at: 2628 2628
Size: 6226
EcoRI G'AATT_C
Cuts at: 3535 3913 3535
Size: 378 5848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 2864 2471
Size: 393 5833
FspI TGC'GCA
Cuts at: 4732 4732
Size: 6226
HaeII r_GCGC'y
Cuts at: 1678 2731 5373 1678
Size: 1053 2642 2531
Fragments arranged by size:
2642 2531 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2731 2763 4867 4899 4941
Size: 32 135 32 1159 32 2104 32 42
Cuts at: 4941 4973 1373
Size: 32 2626
Fragments arranged by size:
2626 2104 1159 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3570 3816 669
Size: 988 281 1632 246 3079
Fragments arranged by size:
3079 1632 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3142 3188 748
Size: 2394 46 3786
Fragments arranged by size:
3786 2394 46
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3351 3825 3351
Size: 474 5752
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2331 5340 5524 1305
Size: 1026 3009 184 2007
Fragments arranged by size:
3009 2007 1026 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3046 3273 10
Size: 54 1425 343 1214 227 2963
Fragments arranged by size:
2963 1425 1214 343 227 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3040 4184 4543 4702 518
Size: 648 1874 1144 359 159 2042
Fragments arranged by size:
2042 1874 1144 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2042 2058 2719 3642 4319 5467 5712 1606
Size: 436 16 661 923 677 1148 245 2120
Fragments arranged by size:
2120 1148 923 677 661 436 245 16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5674
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 2639 3313 3584 5129 1132
Size: 97 1410 674 271 1545 2229
Fragments arranged by size:
2229 1545 1410 674 271 97
PasI CC'CwG_GG
Cuts at: 1348 3134 1348
Size: 1786 4440
PciI A'CATG_T
Cuts at: 5125 5125
Size: 6226
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2293 3053 1108
Size: 902 283 760 4281
Fragments arranged by size:
4281 902 760 283
PfoI T'CCnGG_A
Cuts at: 2197 2197
Size: 6226
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2065 2097 2065
Size: 32 6194
PstI C_TGCA'G
Cuts at: 830 2755 3333 3475 3578 830
Size: 1925 578 142 103 3478
Fragments arranged by size:
3478 1925 578 142 103
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
PvuII CAG'CTG
Cuts at: 2719 2719
Size: 6226
SacII CC_GC'GG
Cuts at: 2059 2059
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SfcI C'TryA_G
Cuts at: 826 1045 2043 2751 3157 3329 3471 3574 4709
Size: 219 998 708 406 172 142 103 1135
Cuts at: 4709 5390 5581 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 998 708 681 406 219 191
172 142 103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 4330 5231 5493 820
Size: 197 926 322 2065 901 262 1553
Fragments arranged by size:
2065 1553 926 901 322 262 197
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 3584 1132
Size: 2452 3774
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6226
StyI C'CwwG_G
Cuts at: 513 1065 2009 3665 513
Size: 552 944 1656 3074
Fragments arranged by size:
3074 1656 944 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2181 2919 4253 4270 4423 4608 5053
Size: 1255 601 738 1334 17 153 185 445
Cuts at: 5053 325
Size: 1498
Fragments arranged by size:
1498 1334 1255 738 601 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4472 6184 96
Size: 274 80 33 51 1443 2495 1712 138
Fragments arranged by size:
2495 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3447 3479 6186 6218 3447
Size: 32 2707 32 3455
Fragments arranged by size:
3455 2707 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6226
XbaI T'CTAG_A
Cuts at: 3097 3562 3097
Size: 465 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6226
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 2565 2844 3093 4355 2565
Size: 279 249 1262 4436
Fragments arranged by size:
4436 1262 279 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 276
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BstXI BstZ17I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI