Restriction map for
pFN21AE0338
(Circular) (Six-base) MAPSORT of: pFN21AE0338.seq Check: 2066 from: 1 to: 6220
pFN21AE0338 6220 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2586 2945 4283 5667 5935 1632
Size: 714 240 359 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 714 359 268 240
AflII C'TTAA_G
Cuts at: 820 1017 2542 820
Size: 197 1525 4498
Fragments arranged by size:
4498 1525 197
AflIII A'CryG_T
Cuts at: 1203 3040 5119 6096 1203
Size: 1837 2079 977 1327
Fragments arranged by size:
2079 1837 1327 977
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AlwNI CAG_nnn'CTG
Cuts at: 2942 3963 5535 2942
Size: 1021 1572 3627
Fragments arranged by size:
3627 1572 1021
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3309 3545 1870
Size: 73 1366 236 4545
Fragments arranged by size:
4545 1366 236 73
BamHI G'GATC_C
Cuts at: 3131 3550 3917 3131
Size: 419 367 5434
Fragments arranged by size:
5434 419 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3212 3541 4996 618
Size: 325 217 627 1425 329 1455 1842
Fragments arranged by size:
1842 1455 1425 627 329 325 217
BanII G_rGCy'C
Cuts at: 1391 3539 1391
Size: 2148 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 3001 928
Size: 971 675 427 4147
Fragments arranged by size:
4147 971 675 427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4411 4445 1903
Size: 34 2474 34 3678
Fragments arranged by size:
3678 2474 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2347 4112 5322 2347
Size: 1765 1210 3245
Fragments arranged by size:
3245 1765 1210
BclI T'GATC_A
Cuts at: 1548 3510 1548
Size: 1962 4258
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2088 4281 5437 1790
Size: 298 2193 1156 2573
Fragments arranged by size:
2573 2193 1156 298
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2083 2150 4880 1239
Size: 844 67 2730 2579
Fragments arranged by size:
2730 2579 844 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3260 3490 4883 882
Size: 617 1761 230 1393 2219
Fragments arranged by size:
2219 1761 1393 617 230
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4027 4409 5044 275
Size: 53 83 186 936 2494 382 635 1451
Fragments arranged by size:
2494 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2525 2600 3141 1186
Size: 1339 75 541 4265
Fragments arranged by size:
4265 1339 541 75
BseYI C'CCAG_C
Cuts at: 2942 5423 5919 2942
Size: 2481 496 3243
Fragments arranged by size:
3243 2481 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5647
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2580 2893 3204 4281 4366 5437 2580
Size: 313 311 1077 85 1071 3363
Fragments arranged by size:
3363 1077 1071 313 311 85
BsmI GAATG_Cn'
Cuts at: 2164 2500 3731 3824 2164
Size: 336 1231 93 4560
Fragments arranged by size:
4560 1231 336 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2580 2893 3204 3539 4281 4366
Size: 399 298 492 313 311 335 742 85
Cuts at: 4366 5437 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 492 399 335 313 311
298 85
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 3464 3575 844
Size: 1242 1378 111 3489
Fragments arranged by size:
3489 1378 1242 111
BsrBI CCG'CTC
Cuts at: 1394 1823 2967 3293 4107 1394
Size: 429 1144 326 814 3507
Fragments arranged by size:
3507 1144 814 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 2221 3066 3165 4715 4889 65
Size: 1183 973 845 99 1550 174 1396
Fragments arranged by size:
1550 1396 1183 973 845 174 99
BsrFI r'CCGG_y
Cuts at: 1700 1810 4864 5051 1700
Size: 110 3054 187 2869
Fragments arranged by size:
3054 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6220
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 4280 5292 4280
Size: 1012 5208
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6220
BstXI CCAn_nnnn'nTGG
Cuts at: 2506 3188 3467 2506
Size: 682 279 5259
Fragments arranged by size:
5259 682 279
BstYI r'GATC_y
Cuts at: 1972 3131 3550 3917 4302 4319 5771 5857 5869
Size: 1159 419 367 385 17 1452 86 12
Cuts at: 5869 6215 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1159 419 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3216 513
Size: 552 2151 3517
Fragments arranged by size:
3517 2151 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 3736 4530 4550 2578
Size: 1158 794 20 4248
Fragments arranged by size:
4248 1158 794 20
ClaI AT'CG_AT
Cuts at: 1554 2245 1554
Size: 691 5529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3667 4556 6185
Size: 54 1425 50 275 18 1837 889 1629
Cuts at: 6185 8
Size: 43
Fragments arranged by size:
1837 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2843 2974 3405 4150 1353
Size: 1490 131 431 745 3423
Fragments arranged by size:
3423 1490 745 431 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2150 2346 2586 2945 4283 4880
Size: 393 451 67 196 240 359 1338 597
Cuts at: 4880 5667 5935 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1338 787 597 451 393 359 268
240 196 67
EcoO109I rG'GnC_Cy
Cuts at: 2664 2664
Size: 6220
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 5367 1678
Size: 3689 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373
Size: 32 135 32 3289 32 42 32 2626
Fragments arranged by size:
3289 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3564 3810 669
Size: 988 281 1024 602 246 3079
Fragments arranged by size:
3079 1024 988 602 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3501 3819 3501
Size: 318 5902
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4398
Fragments arranged by size:
4398 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 4178 4537 4696 518
Size: 648 1323 1689 359 159 2042
Fragments arranged by size:
2042 1689 1323 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2939 3636 4313 5461 5706 1606
Size: 1333 697 677 1148 245 2120
Fragments arranged by size:
2120 1333 1148 697 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3216 513
Size: 552 2151 3517
Fragments arranged by size:
3517 2151 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2095 3044 3089 3578 5123 1132
Size: 97 866 949 45 489 1545 2229
Fragments arranged by size:
2229 1545 949 866 489 97 45
PasI CC'CwG_GG
Cuts at: 1348 2668 1348
Size: 1320 4900
PciI A'CATG_T
Cuts at: 3040 5119 3040
Size: 2079 4141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4624
PfoI T'CCnGG_A
Cuts at: 3339 3339
Size: 6220
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2018 2050 4289 4321 5802 5834 2018
Size: 32 2239 32 1481 32 2404
Fragments arranged by size:
2404 2239 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 2664
Size: 6220
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PstI C_TGCA'G
Cuts at: 830 2034 2607 3572 830
Size: 1204 573 965 3478
Fragments arranged by size:
3478 1204 965 573
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2939 2939
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SanDI GG'GwC_CC
Cuts at: 2664 2664
Size: 6220
SapI GCTCTTCn'nnn_
Cuts at: 2974 2974
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SfcI C'TryA_G
Cuts at: 826 1045 2030 2603 3226 3568 4703 5384 5575
Size: 219 985 573 623 342 1135 681 191
Cuts at: 5575 826
Size: 1471
Fragments arranged by size:
1471 1135 985 681 623 573 342 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2542 4324 5225 5487 820
Size: 197 926 599 1782 901 262 1553
Fragments arranged by size:
1782 1553 926 901 599 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 2649 1517
Size: 999 133 5088
Fragments arranged by size:
5088 999 133
StyI C'CwwG_G
Cuts at: 513 1065 2517 3003 3216 3659 513
Size: 552 1452 486 213 443 3074
Fragments arranged by size:
3074 1452 552 486 443 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3409 4247 4264 4417 4602 5047 325
Size: 1255 1829 838 17 153 185 445 1498
Fragments arranged by size:
1829 1498 1255 838 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3118 4466 6178
Size: 274 80 33 51 1443 1141 1348 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1712 1443 1348 1141 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3183 3215 6180 6212 3183
Size: 32 2965 32 3191
Fragments arranged by size:
3191 2965 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2276 2459 2821 3403 2276
Size: 183 362 582 5093
Fragments arranged by size:
5093 582 362 183
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 2078 2899 4349 2078
Size: 821 1450 3949
Fragments arranged by size:
3949 1450 821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI