Restriction map for
pFN21AE0337
(Circular) (Six-base) MAPSORT of: pFN21AE0337.seq Check: 5120 from: 1 to: 6232
pFN21AE0337 6232 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4295 5679 5947 1632
Size: 1739 924 1384 268 1917
Fragments arranged by size:
1917 1739 1384 924 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 5131 6108 1203
Size: 3928 977 1327
Fragments arranged by size:
3928 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2739 1184
Size: 1555 4677
AlwNI CAG_nnn'CTG
Cuts at: 2485 2536 3245 3975 5547 2485
Size: 51 709 730 1572 3170
Fragments arranged by size:
3170 1572 730 709 51
ApaI G_GGCC'C
Cuts at: 2474 3049 2474
Size: 575 5657
ApaLI G'TGCA_C
Cuts at: 2215 4289 5445 2215
Size: 2074 1156 3002
Fragments arranged by size:
3002 2074 1156
ApoI r'AATT_y
Cuts at: 1493 3541 3767 3919 6028 1493
Size: 2048 226 152 2109 1697
Fragments arranged by size:
2109 2048 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 2349 2880 3444 3557 1870
Size: 73 355 51 531 564 113 4545
Fragments arranged by size:
4545 564 531 355 113 73 51
BamHI G'GATC_C
Cuts at: 3562 3929 3562
Size: 367 5865
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2130 2287 2382 3553
Size: 325 217 627 230 113 157 95 1171
Cuts at: 3553 5008 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1171 627 325 230 217 157
113 95
BanII G_rGCy'C
Cuts at: 1391 2474 3049 3116 3218 3285 3551 1391
Size: 1083 575 67 102 67 266 4072
Fragments arranged by size:
4072 1083 575 266 102 67 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5261
BbvCI CC'TCA_GC
Cuts at: 3083 3083
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2871 2905 4423 4457 1903
Size: 34 934 34 1518 34 3678
Fragments arranged by size:
3678 1518 934 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2587 4843 136
Size: 107 122 71 631 605 915 2256 1525
Fragments arranged by size:
2256 1525 915 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3468 6227 3468
Size: 2759 3473
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2020 2133 2219 2292 2474 3049 3104 4293
Size: 230 113 86 73 182 575 55 1189
Cuts at: 4293 5449 1790
Size: 1156 2573
Fragments arranged by size:
2573 1189 1156 575 230 182 113 86
73 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3397 3963 4921
Size: 416 53 83 399 24 1980 566 958
Cuts at: 4921 442
Size: 1753
Fragments arranged by size:
1980 1753 958 566 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2375 2375
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 3244 4892 1239
Size: 1097 908 1648 2579
Fragments arranged by size:
2579 1648 1097 908
Bpu10I CC'TnA_GC
Cuts at: 3083 3083
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3380 4357 5222 5520 3380
Size: 977 865 298 4092
Fragments arranged by size:
4092 977 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2858 2903 4895 882
Size: 617 1359 45 1992 2219
Fragments arranged by size:
2219 1992 1359 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 2200 4023 493
Size: 711 996 1823 2702
Fragments arranged by size:
2702 1823 996 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2280 2643 4039 4421
Size: 53 83 186 936 747 363 1396 382
Cuts at: 4421 5056 275
Size: 635 1451
Fragments arranged by size:
1451 1396 936 747 635 382 363 186
83 53
BsaWI w'CCGG_w
Cuts at: 2894 4665 5063 5337 5484 5895 2894
Size: 1771 398 274 147 411 3231
Fragments arranged by size:
3231 1771 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2333 1186
Size: 835 312 5085
Fragments arranged by size:
5085 835 312
BseYI C'CCAG_C
Cuts at: 2135 2291 2646 3041 3097 3334 3431 3499 5435
Size: 156 355 395 56 237 97 68 1936
Cuts at: 5435 5931 2135
Size: 496 2436
Fragments arranged by size:
2436 1936 496 395 355 237 156 97
68 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3472 1163
Size: 573 1736 3923
Fragments arranged by size:
3923 1736 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2248 3682 4443 5471 1540
Size: 275 186 247 1434 761 1028 2301
Fragments arranged by size:
2301 1434 1028 761 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2219 4293 4378 5449 2219
Size: 2074 85 1071 3002
Fragments arranged by size:
3002 2074 1071 85
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
BsmBI CGTCTCn'nnnn_
Cuts at: 2822 2822
Size: 6232
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2133 2219 2292 2474 3049 3104
Size: 399 230 113 86 73 182 575 55
Cuts at: 3104 3116 3218 3285 3551 4293 4378 5449 1391
Size: 12 102 67 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 399 266 230 182
113 102 86 85 73 67 55 12
BspEI T'CCGG_A
Cuts at: 5895 5895
Size: 6232
BspHI T'CATG_A
Cuts at: 3277 4121 5851 3277
Size: 844 1730 3658
Fragments arranged by size:
3658 1730 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2498 3024 3339 3587 844
Size: 1654 526 315 248 3489
Fragments arranged by size:
3489 1654 526 315 248
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3511 4727 4901 65
Size: 1183 2263 1216 174 1396
Fragments arranged by size:
2263 1396 1216 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 2247 2372 2500 4876 5063 1700
Size: 110 433 4 125 128 2376 187 2869
Fragments arranged by size:
2869 2376 433 187 128 125 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 2993 4292 5304 2993
Size: 1299 1012 3921
Fragments arranged by size:
3921 1299 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3245 794
Size: 2451 3781
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 2323 1184
Size: 1139 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3010 3439 2011
Size: 999 429 4804
Fragments arranged by size:
4804 999 429
BstYI r'GATC_y
Cuts at: 1972 3468 3520 3562 3929 4314 4331 5783 5869
Size: 1496 52 42 367 385 17 1452 86
Cuts at: 5869 5881 6227 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1496 1452 385 367 346 86 52
42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2400 2400
Size: 6232
BtgI C'CryG_G
Cuts at: 513 1065 2010 2021 2328 2568 2740 3160 513
Size: 552 945 11 307 240 172 420 3585
Fragments arranged by size:
3585 945 552 420 307 240 172 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 2844 3050 3234 3748 4542 4562 2844
Size: 206 184 514 794 20 4514
Fragments arranged by size:
4514 794 514 206 184 20
ClaI AT'CG_AT
Cuts at: 1554 2437 1554
Size: 883 5349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2245 2331
Size: 54 1425 50 275 18 225 190 86
Cuts at: 2331 2370 2502 2556 2637 2963 3000 3345 3679
Size: 39 132 54 81 326 37 345 334
Cuts at: 3679 4568 6197 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 345 334 326 275 225
190 132 86 81 54 54 50 43
39 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2245 3679 1537
Size: 275 433 1434 4090
Fragments arranged by size:
4090 1434 433 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2820 3134 4162 1353
Size: 1467 314 1028 3423
Fragments arranged by size:
3423 1467 1028 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 4819 5193 5339 387
Size: 1064 138 469 2761 374 146 1280
Fragments arranged by size:
2761 1280 1064 469 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 3244 3371 4295 4892 5679 5947
Size: 393 704 908 127 924 597 787 268
Cuts at: 5947 1239
Size: 1524
Fragments arranged by size:
1524 924 908 787 704 597 393 268
127
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 3305 2318
Size: 987 5245
EcoO109I rG'GnC_Cy
Cuts at: 2113 2305 2396 2406 2470 2471 2533 2787 2809
Size: 192 91 10 64 1 62 254 22
Cuts at: 2809 3045 3046 3310 3454 2113
Size: 236 1 264 144 4891
Fragments arranged by size:
4891 264 254 236 192 144 91 64
62 22 10 1 1
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 6232
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2653 5379 1678
Size: 975 2726 2531
Fragments arranged by size:
2726 2531 975
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3460 3492 4873 4905 4947
Size: 32 135 32 1888 32 1381 32 42
Cuts at: 4947 4979 1373
Size: 32 2626
Fragments arranged by size:
2626 1888 1381 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3576 3822 669
Size: 988 281 1638 246 3079
Fragments arranged by size:
3079 1638 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5346 5530 1305
Size: 4041 184 2007
Fragments arranged by size:
4041 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 2965 3002 10
Size: 54 1425 343 726 407 37 3240
Fragments arranged by size:
3240 1425 726 407 343 54 37
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2366 2873 4190 4549 4708 518
Size: 648 843 357 507 1317 359 159 2042
Fragments arranged by size:
2042 1317 843 648 507 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2164 2742 2780 3289 3391 3416 3648
Size: 417 141 578 38 509 102 25 232
Cuts at: 3648 4325 5473 5718 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 578 509 417 245 232
141 102 38 25
NaeI GCC'GGC
Cuts at: 2249 2374 2502 2249
Size: 125 128 5979
Fragments arranged by size:
5979 128 125
NcoI C'CATG_G
Cuts at: 513 1065 2010 513
Size: 552 945 4735
Fragments arranged by size:
4735 945 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NgoMIV G'CCGG_C
Cuts at: 2247 2372 2500 2247
Size: 125 128 5979
Fragments arranged by size:
5979 128 125
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2272 3590 5135 1132
Size: 97 1043 1318 1545 2229
Fragments arranged by size:
2229 1545 1318 1043 97
PasI CC'CwG_GG
Cuts at: 1348 3211 1348
Size: 1863 4369
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2536 2998 1108
Size: 1428 462 4342
Fragments arranged by size:
4342 1428 462
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 2787 2809 3310 3454 2113
Size: 674 22 501 144 4891
Fragments arranged by size:
4891 674 501 144 22
PshAI GACnn'nnGTC
Cuts at: 1613 2834 1613
Size: 1221 5011
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2470 3045 2470
Size: 575 5657
PstI C_TGCA'G
Cuts at: 830 2851 3241 3468 3584 830
Size: 2021 390 227 116 3478
Fragments arranged by size:
3478 2021 390 227 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2164 2780 3391 3416 2164
Size: 616 611 25 4980
Fragments arranged by size:
4980 616 611 25
SacII CC_GC'GG
Cuts at: 2024 2743 2024
Size: 719 5513
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SanDI GG'GwC_CC
Cuts at: 2787 2787
Size: 6232
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SfcI C'TryA_G
Cuts at: 826 1045 2847 3237 3464 3580 4715 5396 5587
Size: 219 1802 390 227 116 1135 681 191
Cuts at: 5587 826
Size: 1471
Fragments arranged by size:
1802 1471 1135 681 390 227 219 191
116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 2300 3446 3559 1872
Size: 428 1146 113 4545
Fragments arranged by size:
4545 1146 428 113
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3395 4336 5237 5499 820
Size: 197 926 1452 941 901 262 1553
Fragments arranged by size:
1553 1452 941 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2010 2386 2952 3066 3306 3671 513
Size: 552 945 376 566 114 240 365 3074
Fragments arranged by size:
3074 945 566 552 376 365 240 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2708 3224 4259 4276 4429 4614 5059
Size: 1255 1128 516 1035 17 153 185 445
Cuts at: 5059 325
Size: 1498
Fragments arranged by size:
1498 1255 1128 1035 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 3189 4478
Size: 274 80 33 51 1443 696 516 1289
Cuts at: 4478 6190 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1289 696 516 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 3155 1316
Size: 1839 4393
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2551 2566 2809 3064 2152
Size: 399 15 243 255 5320
Fragments arranged by size:
5320 399 255 243 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 2298 3444 3557 1870
Size: 428 1146 113 4545
Fragments arranged by size:
4545 1146 428 113
XmnI GAAnn'nnTTC
Cuts at: 4361 4361
Size: 6232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI