Restriction map for
pFN21AE0336
(Circular) (Six-base) MAPSORT of: pFN21AE0336.seq Check: 1720 from: 1 to: 6169
pFN21AE0336 6169 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3432 3432
Size: 6169
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3171 3979 278
Size: 53 83 186 936 1635 808 2468
Fragments arranged by size:
2468 1635 936 808 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 3512 1937
Size: 919 656 4594
Fragments arranged by size:
4594 919 656
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4232 5616 5884 1632
Size: 2600 1384 268 1917
Fragments arranged by size:
2600 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 5068 6045 1203
Size: 3865 977 1327
Fragments arranged by size:
3865 1327 977
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2099 3380 1166
Size: 933 1281 3955
Fragments arranged by size:
3955 1281 933
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2744 2776 2744
Size: 32 6137
AlwNI CAG_nnn'CTG
Cuts at: 2985 3139 3237 3912 5484 2985
Size: 154 98 675 1572 3670
Fragments arranged by size:
3670 1572 675 154 98
ApaI G_GGCC'C
Cuts at: 2515 2515
Size: 6169
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 3494 1870
Size: 73 267 1284 4545
Fragments arranged by size:
4545 1284 267 73
BamHI G'GATC_C
Cuts at: 2655 3499 3866 2655
Size: 844 367 4958
Fragments arranged by size:
4958 844 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3151 3490 4945 618
Size: 325 217 627 1364 339 1455 1842
Fragments arranged by size:
1842 1455 1364 627 339 325 217
BanII G_rGCy'C
Cuts at: 1391 2515 2632 3324 3488 1391
Size: 1124 117 692 164 4072
Fragments arranged by size:
4072 1124 692 164 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2381 2489 2648
Size: 107 122 71 631 605 709 108 159
Cuts at: 2648 4780 136
Size: 2132 1525
Fragments arranged by size:
2132 1525 709 631 605 159 122 108
107 71
BglII A'GATC_T
Cuts at: 6164 6164
Size: 6169
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2515 3156 4230 5386 1790
Size: 725 641 1074 1156 2573
Fragments arranged by size:
2573 1156 1074 725 641
BmgBI CAC'GTC
Cuts at: 1318 3187 1318
Size: 1869 4300
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2776 2900 3900
Size: 416 53 83 399 24 1359 124 1000
Cuts at: 3900 4858 442
Size: 958 1753
Fragments arranged by size:
1753 1359 1000 958 416 399 124 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2221 3259 3467 4829 1239
Size: 941 41 1038 208 1362 2579
Fragments arranged by size:
2579 1362 1038 941 208 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2198 4294 5159 5457 2198
Size: 2096 865 298 2910
Fragments arranged by size:
2910 2096 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3042 4832 882
Size: 617 1543 1790 2219
Fragments arranged by size:
2219 1790 1543 617
BsaAI yAC'GTr
Cuts at: 493 1204 2254 3960 493
Size: 711 1050 1706 2702
Fragments arranged by size:
2702 1706 1050 711
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3123 3168 3976 4358
Size: 53 83 186 936 1590 45 808 382
Cuts at: 4358 4993 275
Size: 635 1451
Fragments arranged by size:
1590 1451 936 808 635 382 186 83
53 45
BsaWI w'CCGG_w
Cuts at: 4602 5000 5274 5421 5832 4602
Size: 398 274 147 411 4939
Fragments arranged by size:
4939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 1178
Size: 30 165 30 1341 30 4573
Fragments arranged by size:
4573 1341 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2319 1186
Size: 934 199 5036
Fragments arranged by size:
5036 934 199
BseYI C'CCAG_C
Cuts at: 2044 2397 5372 5868 2044
Size: 353 2975 496 2345
Fragments arranged by size:
2975 2345 496 353
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2419 3125 3161 3284 1163
Size: 573 683 706 36 123 4048
Fragments arranged by size:
4048 706 683 573 123 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3464 3619 4380 5408 1540
Size: 275 186 1463 155 761 1028 2301
Fragments arranged by size:
2301 1463 1028 761 275 186 155
BsiHKAI G_wGCw'C
Cuts at: 4230 4315 5386 4230
Size: 85 1071 5013
Fragments arranged by size:
5013 1071 85
BsmI GAATG_Cn'
Cuts at: 3073 3680 3773 3073
Size: 607 93 5469
Fragments arranged by size:
5469 607 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2353 2353
Size: 6169
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2515 2632 3156 3324 3488 4230 4315
Size: 399 725 117 524 168 164 742 85
Cuts at: 4315 5386 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 725 524 399 168 164
117 85
BspEI T'CCGG_A
Cuts at: 5832 5832
Size: 6169
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2127 2145 3003 3432 3524 844
Size: 1283 18 858 429 92 3489
Fragments arranged by size:
3489 1283 858 429 92 18
BsrBI CCG'CTC
Cuts at: 1394 1823 4056 1394
Size: 429 2233 3507
Fragments arranged by size:
3507 2233 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3135 4664 4838 65
Size: 1183 1887 1529 174 1396
Fragments arranged by size:
1887 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4813 5000 1700
Size: 110 3003 187 2869
Fragments arranged by size:
3003 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3024 96
Size: 2928 3241
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 2504 3059 4229 5241 2504
Size: 555 1170 1012 3432
Fragments arranged by size:
3432 1170 1012 555
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3139 794
Size: 2345 3824
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 3367 2458
Size: 909 5260
BstYI r'GATC_y
Cuts at: 1972 2655 3499 3866 4251 4268 5720 5806 5818
Size: 683 844 367 385 17 1452 86 12
Cuts at: 5818 6164 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 844 683 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 2376 2812 3084 513
Size: 552 939 240 132 436 272 3598
Fragments arranged by size:
3598 939 552 436 272 240 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 2235 2444 3685 4479 4499 2235
Size: 209 1241 794 20 3905
Fragments arranged by size:
3905 1241 794 209 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6134
DraI TTT'AAA
Cuts at: 3473 3473
Size: 6169
DraIII CAC_nnn'GTG
Cuts at: 3061 3061
Size: 6169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3208 5176 809
Size: 507 1892 1968 1802
Fragments arranged by size:
1968 1892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2382 2406
Size: 54 1425 50 275 18 228 324 24
Cuts at: 2406 2714 3357 3461 3616 4505 6134 8
Size: 308 643 104 155 889 1629 43
Fragments arranged by size:
1629 1425 889 643 324 308 275 228
155 104 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3461 3616 1537
Size: 275 1649 155 4090
Fragments arranged by size:
4090 1649 275 155
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 4099 1353
Size: 1487 1259 3423
Fragments arranged by size:
3423 1487 1259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2221 3259 3467 4232 4829 5616
Size: 393 548 41 1038 208 765 597 787
Cuts at: 5616 5884 1239
Size: 268 1524
Fragments arranged by size:
1524 1038 787 765 597 548 393 268
208 41
EcoNI CCTnn'n_nnAGG
Cuts at: 3305 3305
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2772 3352 2772
Size: 580 5589
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 3237 5316 1678
Size: 1559 2079 2531
Fragments arranged by size:
2531 2079 1559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4810 4842 4884 4916 1373
Size: 32 135 32 3238 32 42 32 2626
Fragments arranged by size:
3238 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3229 3513 3759 669
Size: 988 281 1291 284 246 3079
Fragments arranged by size:
3079 1291 988 284 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3768 3768
Size: 6169
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 5283 5467 1305
Size: 1482 2496 184 2007
Fragments arranged by size:
2496 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2384 3359 10
Size: 54 1425 343 552 975 2820
Fragments arranged by size:
2820 1425 975 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2546 3185 3302 3380 4127 4486
Size: 648 933 447 639 117 78 747 359
Cuts at: 4486 4645 518
Size: 159 2042
Fragments arranged by size:
2042 933 747 648 639 447 359 159
117 78
MspA1I CmG'CkG
Cuts at: 1606 2246 2782 2814 3148 3585 4262 5410 5655
Size: 640 536 32 334 437 677 1148 245
Cuts at: 5655 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 640 536 437 334 245
32
NcoI C'CATG_G
Cuts at: 513 1065 2004 2376 513
Size: 552 939 372 4306
Fragments arranged by size:
4306 939 552 372
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 3283 3527 5072 1132
Size: 97 2054 244 1545 2229
Fragments arranged by size:
2229 2054 1545 244 97
PasI CC'CwG_GG
Cuts at: 1348 2845 3317 1348
Size: 1497 472 4200
Fragments arranged by size:
4200 1497 472
PciI A'CATG_T
Cuts at: 5068 5068
Size: 6169
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6169
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2744 2776 4238 4270 5751 5783 2744
Size: 32 1462 32 1481 32 3130
Fragments arranged by size:
3130 1481 1462 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2772 3352 2772
Size: 580 5589
PshAI GACnn'nnGTC
Cuts at: 1613 3167 1613
Size: 1554 4615
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PspOMI G'GGCC_C
Cuts at: 2511 2511
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2124 2828 3429 3521 830
Size: 1294 704 601 92 3478
Fragments arranged by size:
3478 1294 704 601 92
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 3148 3148
Size: 6169
SacII CC_GC'GG
Cuts at: 2247 2815 2247
Size: 568 5601
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SapI GCTCTTCn'nnn_
Cuts at: 2840 2840
Size: 6169
SbfI CC_TGCA'GG
Cuts at: 2124 3521 2124
Size: 1397 4772
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SexAI A'CCwGG_T
Cuts at: 2076 2076
Size: 6169
SfcI C'TryA_G
Cuts at: 826 1045 2120 2469 2619 2824 3334 3425 3517
Size: 219 1075 349 150 205 510 91 92
Cuts at: 3517 4652 5333 5524 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 510 349 219 205
191 150 92 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 2381 2489 2381
Size: 108 6061
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 3496 1872
Size: 1624 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 4273 5174 5436 820
Size: 197 926 234 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 234 197
SnaBI TAC'GTA
Cuts at: 493 3960 493
Size: 3467 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3283 3527 1132
Size: 2151 244 3774
Fragments arranged by size:
3774 2151 244
SspI AAT'ATT
Cuts at: 5 52 4093 5
Size: 47 4041 2081
Fragments arranged by size:
4041 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 2598 3608 513
Size: 552 939 372 222 1010 3074
Fragments arranged by size:
3074 1010 939 552 372 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2389 4196 4213 4366 4551 4996 325
Size: 1255 809 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1498 1255 809 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3024 4415 6127
Size: 274 80 33 51 1443 1047 1391 1712
Cuts at: 6127 96
Size: 138
Fragments arranged by size:
1712 1443 1391 1047 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 3045 3077 3336 3364 3368 3396 6129
Size: 32 706 32 259 28 4 28 2733
Cuts at: 6129 6161 2307
Size: 32 2315
Fragments arranged by size:
2733 2315 706 259 32 32 32 28
28 4
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 3494 1870
Size: 1624 4545
XmnI GAAnn'nnTTC
Cuts at: 2614 4298 2614
Size: 1684 4485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3169 3977 276
Size: 53 83 186 936 1635 808 2468
Fragments arranged by size:
2468 1635 936 808 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SacI SanDI SfoI
SrfI SwaI