Restriction map for
pFN21AE0331
(Circular) (Six-base) MAPSORT of: pFN21AE0331.seq Check: 547 from: 1 to: 6667
pFN21AE0331 6667 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4477 278
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3672 4010 1937
Size: 1735 338 4594
Fragments arranged by size:
4594 1735 338
Acc65I G'GTAC_C
Cuts at: 3988 3988
Size: 6667
AclI AA'CG_TT
Cuts at: 1563 4794 5167 1563
Size: 3231 373 3063
Fragments arranged by size:
3231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 2640 2727 3018 4730 6114 6382 1632
Size: 945 63 87 291 1712 1384 268 1917
Fragments arranged by size:
1917 1712 1384 945 291 268 87 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6470
AflIII A'CryG_T
Cuts at: 1203 5566 6543 1203
Size: 4363 977 1327
Fragments arranged by size:
4363 1327 977
AgeI A'CCGG_T
Cuts at: 5498 5498
Size: 6667
AhdI GACnn_n'nnGTC
Cuts at: 2058 5396 2058
Size: 3338 3329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6667
AlwNI CAG_nnn'CTG
Cuts at: 3460 3814 4410 5982 3460
Size: 354 596 1572 4145
Fragments arranged by size:
4145 1572 596 354
ApaLI G'TGCA_C
Cuts at: 4724 5880 4724
Size: 1156 5511
ApoI r'AATT_y
Cuts at: 1493 2012 2322 3500 3603 3655 3853 3976 4202
Size: 519 310 1178 103 52 198 123 226
Cuts at: 4202 4354 6463 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1178 519 310 226 198 152
123 103 52
AseI AT'TA_AT
Cuts at: 160 1032 2990 5221 160
Size: 872 1958 2231 1606
Fragments arranged by size:
2231 1958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
AvaI C'yCGr_G
Cuts at: 1870 1943 3992 1870
Size: 73 2049 4545
Fragments arranged by size:
4545 2049 73
BamHI G'GATC_C
Cuts at: 3997 4364 3997
Size: 367 6300
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3988 5443 618
Size: 325 217 627 2201 1455 1842
Fragments arranged by size:
2201 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3986 1391
Size: 2595 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2351 2535 3756 928
Size: 971 452 184 1221 3839
Fragments arranged by size:
3839 1221 971 452 184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3668 3702 4858 4892 1903
Size: 34 1731 34 1156 34 3678
Fragments arranged by size:
3678 1731 1156 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2968 4559 5769 2968
Size: 1591 1210 3866
Fragments arranged by size:
3866 1591 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6667
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5278 136
Size: 107 122 71 631 605 3606 1525
Fragments arranged by size:
3606 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3707 6662 3707
Size: 2955 3712
BlpI GC'TnA_GC
Cuts at: 4084 4084
Size: 6667
Bme1580I G_kGCm'C
Cuts at: 1790 4728 5884 1790
Size: 2938 1156 2573
Fragments arranged by size:
2938 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2599 4398 5356
Size: 416 53 83 399 24 1182 1799 958
Cuts at: 5356 442
Size: 1753
Fragments arranged by size:
1799 1753 1182 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2688 3516 5327 1239
Size: 845 604 828 1811 2579
Fragments arranged by size:
2579 1811 845 828 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3107 3136 4792 5657 5955 3107
Size: 29 1656 865 298 3819
Fragments arranged by size:
3819 1656 865 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3583 5330 882
Size: 617 2084 1747 2219
Fragments arranged by size:
2219 2084 1747 617
BsaAI yAC'GTr
Cuts at: 493 1204 4458 493
Size: 711 3254 2702
Fragments arranged by size:
3254 2702 711
BsaBI GATnn'nnATC
Cuts at: 3516 6661 3516
Size: 3145 3522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4474 4856 5491 275
Size: 53 83 186 936 2941 382 635 1451
Fragments arranged by size:
2941 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5100 5498 5772 5919 6330 5100
Size: 398 274 147 411 5437
Fragments arranged by size:
5437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6442
Fragments arranged by size:
6442 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2709 1186
Size: 1523 5144
BseYI C'CCAG_C
Cuts at: 5870 6366 5870
Size: 496 6171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6094
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4117 4878 5906 1540
Size: 275 186 2116 761 1028 2301
Fragments arranged by size:
2301 2116 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2904 4728 4813 5884 2904
Size: 1824 85 1071 3687
Fragments arranged by size:
3687 1824 1071 85
BsmI GAATG_Cn'
Cuts at: 4178 4271 4178
Size: 93 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2904 3986 4728 4813 5884 1391
Size: 399 1114 1082 742 85 1071 2174
Fragments arranged by size:
2174 1114 1082 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6330 6330
Size: 6667
BspHI T'CATG_A
Cuts at: 3849 4556 6286 3849
Size: 707 1730 4230
Fragments arranged by size:
4230 1730 707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4022 844
Size: 3178 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4554 1394
Size: 429 2731 3507
Fragments arranged by size:
3507 2731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5162 5336 65
Size: 1183 3914 174 1396
Fragments arranged by size:
3914 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5311 5498 1700
Size: 110 3501 187 2869
Fragments arranged by size:
3501 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2740 3068 96
Size: 2644 328 3695
Fragments arranged by size:
3695 2644 328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6667
BssSI C'ACGA_G
Cuts at: 4727 5739 4727
Size: 1012 5655
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6667
BstBI TT'CG_AA
Cuts at: 3659 5471 3659
Size: 1812 4855
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6667
BstYI r'GATC_y
Cuts at: 1972 3707 3997 4364 4749 4766 6218 6304 6316
Size: 1735 290 367 385 17 1452 86 12
Cuts at: 6316 6662 1972
Size: 346 1977
Fragments arranged by size:
1977 1735 1452 385 367 346 290 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5176
Fragments arranged by size:
5176 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5928
Fragments arranged by size:
5928 683 56
BtsI GCAGTG_nn'
Cuts at: 2781 4183 4977 4997 2781
Size: 1402 794 20 4451
Fragments arranged by size:
4451 1402 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6632
DraI TTT'AAA
Cuts at: 3907 3971 3907
Size: 64 6603
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5674 809
Size: 507 4358 1802
Fragments arranged by size:
4358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4114 5003 6632
Size: 54 1425 50 275 18 2284 889 1629
Cuts at: 6632 8
Size: 43
Fragments arranged by size:
2284 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4114 1537
Size: 275 2302 4090
Fragments arranged by size:
4090 2302 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2295 2703 4597 1353
Size: 837 105 408 1894 3423
Fragments arranged by size:
3423 1894 837 408 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5254 5628 5774 387
Size: 1064 138 3665 374 146 1280
Fragments arranged by size:
3665 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2577 2640 2688 2727 3018 3516
Size: 393 452 493 63 48 39 291 498
Cuts at: 3516 4730 5327 6114 6382 1239
Size: 1214 597 787 268 1524
Fragments arranged by size:
1524 1214 787 597 498 493 452 393
291 268 63 48 39
EcoO109I rG'GnC_Cy
Cuts at: 2159 3683 2159
Size: 1524 5143
EcoRI G'AATT_C
Cuts at: 3603 3853 3976 4354 3603
Size: 250 123 378 5916
Fragments arranged by size:
5916 378 250 123
EcoRV GAT'ATC
Cuts at: 2202 2563 2202
Size: 361 6306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3918 3918
Size: 6667
FspI TGC'GCA
Cuts at: 5173 5173
Size: 6667
HaeII r_GCGC'y
Cuts at: 1678 5814 1678
Size: 4136 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2274 2306 2441
Size: 32 135 32 472 32 198 32 135
Cuts at: 2441 2473 3448 3480 3563 3595 5308 5340 5382
Size: 32 975 32 83 32 1713 32 42
Cuts at: 5382 5414 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 975 472 198 135 135 83
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 4011 4257 669
Size: 988 281 396 1677 246 3079
Fragments arranged by size:
3079 1677 988 396 281 246
HindIII A'AGCT_T
Cuts at: 748 2118 748
Size: 1370 5297
HpaI GTT'AAC
Cuts at: 4257 4257
Size: 6667
KpnI G_GTAC'C
Cuts at: 3992 3992
Size: 6667
MfeI C'AATT_G
Cuts at: 3870 4266 3870
Size: 396 6271
MluI A'CGCG_T
Cuts at: 6543 6543
Size: 6667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5781 5965 1305
Size: 4476 184 2007
Fragments arranged by size:
4476 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4845
Fragments arranged by size:
4845 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4625 4984 5143 518
Size: 648 3459 359 159 2042
Fragments arranged by size:
3459 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2660 4083 4760 5908 6153 1606
Size: 1054 1423 677 1148 245 2120
Fragments arranged by size:
2120 1423 1148 1054 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5176
Fragments arranged by size:
5176 939 552
NdeI CA'TA_TG
Cuts at: 387 3169 387
Size: 2782 3885
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6667
NotI GC'GGCC_GC
Cuts at: 4114 4114
Size: 6667
NspI r_CATG'y
Cuts at: 1132 1229 2645 4025 5570 1132
Size: 97 1416 1380 1545 2229
Fragments arranged by size:
2229 1545 1416 1380 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6667
PciI A'CATG_T
Cuts at: 5566 5566
Size: 6667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2928 1108
Size: 1820 4847
PmeI GTTT'AAAC
Cuts at: 3971 3971
Size: 6667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4736 4768 6249 6281 4736
Size: 32 1481 32 5122
Fragments arranged by size:
5122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2159 2159
Size: 6667
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6667
PsiI TTA'TAA
Cuts at: 4237 4237
Size: 6667
PstI C_TGCA'G
Cuts at: 830 4019 830
Size: 3189 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6667
PvuII CAG'CTG
Cuts at: 2660 2660
Size: 6667
SalI G'TCGA_C
Cuts at: 1936 4009 1936
Size: 2073 4594
SbfI CC_TGCA'GG
Cuts at: 4019 4019
Size: 6667
ScaI AGT'ACT
Cuts at: 2518 4915 6627 2518
Size: 2397 1712 2558
Fragments arranged by size:
2558 2397 1712
SfcI C'TryA_G
Cuts at: 826 1045 4015 5150 5831 6022 826
Size: 219 2970 1135 681 191 1471
Fragments arranged by size:
2970 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6667
SmaI CCC'GGG
Cuts at: 1872 3994 1872
Size: 2122 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3115 3122 4771 5672 5934 820
Size: 197 926 1172 7 1649 901 262 1553
Fragments arranged by size:
1649 1553 1172 926 901 262 197 7
SnaBI TAC'GTA
Cuts at: 493 4458 493
Size: 3965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6667
SphI G_CATG'C
Cuts at: 1132 4025 1132
Size: 2893 3774
SspI AAT'ATT
Cuts at: 5 52 3078 4591 5
Size: 47 3026 1513 2081
Fragments arranged by size:
3026 2081 1513 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6667
StyI C'CwwG_G
Cuts at: 513 1065 2004 2867 3174 3404 4106 513
Size: 552 939 863 307 230 702 3074
Fragments arranged by size:
3074 939 863 702 552 307 230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4694 4711 4864 5049 5494 325
Size: 1255 3114 17 153 185 445 1498
Fragments arranged by size:
3114 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 2516 2740
Size: 274 80 33 51 1443 92 447 224
Cuts at: 2740 3068 4913 6625 96
Size: 328 1845 1712 138
Fragments arranged by size:
1845 1712 1443 447 328 274 224 138
92 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 6627 6659 2225
Size: 32 4370 32 2233
Fragments arranged by size:
4370 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2875 1316
Size: 1559 5108
XbaI T'CTAG_A
Cuts at: 4003 4003
Size: 6667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3457 3457
Size: 6667
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6667
XmaI C'CCGG_G
Cuts at: 1870 3992 1870
Size: 2122 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 4796 3380
Size: 1416 5251
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4475 276
Size: 53 83 186 936 2941 2468
Fragments arranged by size:
2941 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI