Restriction map for
pFN21AE0329
(Circular) (Six-base) MAPSORT of: pFN21AE0329.seq Check: 9299 from: 1 to: 6229
pFN21AE0329 6229 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2075 2075
Size: 6229
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4039 278
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2102 2399 3572 1937
Size: 165 297 1173 4594
Fragments arranged by size:
4594 1173 297 165
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3315 4292 5676 5944 1632
Size: 1683 977 1384 268 1917
Fragments arranged by size:
1917 1683 1384 977 268
AfeI AGC'GCT
Cuts at: 3169 3169
Size: 6229
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 5128 6105 1203
Size: 3925 977 1327
Fragments arranged by size:
3925 1327 977
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3066 1184
Size: 1882 4347
AlwNI CAG_nnn'CTG
Cuts at: 2504 3972 5544 2504
Size: 1468 1572 3189
Fragments arranged by size:
3189 1572 1468
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 2265 2319 2967 3113 3453 3511 3538 3764
Size: 772 54 648 146 340 58 27 226
Cuts at: 3764 3916 6025 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 772 648 340 226 152 146
58 54 27
AseI AT'TA_AT
Cuts at: 160 1032 3287 4783 160
Size: 872 2255 1496 1606
Fragments arranged by size:
2255 1606 1496 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2796 3554 1870
Size: 73 853 758 4545
Fragments arranged by size:
4545 853 758 73
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 3550 5005 618
Size: 325 217 627 234 1529 1455 1842
Fragments arranged by size:
1842 1529 1455 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 3548 1391
Size: 2157 4072
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 6229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2059 2972 3202 928
Size: 971 160 913 230 3955
Fragments arranged by size:
3955 971 913 230 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4420 4454 1903
Size: 34 50 34 2399 34 3678
Fragments arranged by size:
3678 2399 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BfrBI ATG'CAT
Cuts at: 2341 2341
Size: 6229
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2030 4840 136
Size: 107 122 71 631 605 358 2810 1525
Fragments arranged by size:
2810 1525 631 605 358 122 107 71
BglII A'GATC_T
Cuts at: 6224 6224
Size: 6229
BlpI GC'TnA_GC
Cuts at: 3646 3646
Size: 6229
Bme1580I G_kGCm'C
Cuts at: 1790 4290 5446 1790
Size: 2500 1156 2573
Fragments arranged by size:
2573 2500 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6229
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3960 4918 442
Size: 416 53 83 399 24 2543 958 1753
Fragments arranged by size:
2543 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2903 1056
Size: 1847 4382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2862 2862
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2777 3301 4889 1239
Size: 1538 524 1588 2579
Fragments arranged by size:
2579 1588 1538 524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3456 4354 5219 5517 3456
Size: 898 865 298 4168
Fragments arranged by size:
4168 898 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2566 4892 882
Size: 617 1067 2326 2219
Fragments arranged by size:
2326 2219 1067 617
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 4036 4418 5053
Size: 53 83 186 936 489 2014 382 635
Cuts at: 5053 275
Size: 1451
Fragments arranged by size:
2014 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6004
Fragments arranged by size:
6004 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2529 2701 3015 1186
Size: 1343 172 314 4400
Fragments arranged by size:
4400 1343 314 172
BseYI C'CCAG_C
Cuts at: 5432 5928 5432
Size: 496 5733
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2544 2853 3036 1163
Size: 573 808 309 183 4356
Fragments arranged by size:
4356 808 573 309 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 3679 4440 5468 1540
Size: 275 186 33 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 4290 4375 5446 4290
Size: 85 1071 5073
Fragments arranged by size:
5073 1071 85
BsmI GAATG_Cn'
Cuts at: 2757 3740 3833 2757
Size: 983 93 5153
Fragments arranged by size:
5153 983 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3548 4290 4375 5446 1391
Size: 399 1758 742 85 1071 2174
Fragments arranged by size:
2174 1758 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2075 2584 3584 844
Size: 1231 509 1000 3489
Fragments arranged by size:
3489 1231 1000 509
BsrBI CCG'CTC
Cuts at: 1394 1823 2058 4116 1394
Size: 429 235 2058 3507
Fragments arranged by size:
3507 2058 429 235
BsrDI GCAATG_nn'
Cuts at: 65 1248 4724 4898 65
Size: 1183 3476 174 1396
Fragments arranged by size:
3476 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4873 5060 1700
Size: 110 3063 187 2869
Fragments arranged by size:
3063 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3214 96
Size: 3118 3111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6229
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2531 794
Size: 1737 4492
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstYI r'GATC_y
Cuts at: 1972 3559 3926 4311 4328 5780 5866 5878 6224
Size: 1587 367 385 17 1452 86 12 346
Cuts at: 6224 1972
Size: 1977
Fragments arranged by size:
1977 1587 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5677
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2023 487
Size: 683 56 797 4693
Fragments arranged by size:
4693 797 683 56
BtsI GCAGTG_nn'
Cuts at: 3745 4539 4559 3745
Size: 794 20 5415
Fragments arranged by size:
5415 794 20
ClaI AT'CG_AT
Cuts at: 1554 2178 1554
Size: 624 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3439 3533 3439
Size: 94 6135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2046 3676
Size: 54 1425 50 275 18 201 15 1630
Cuts at: 3676 4565 6194 8
Size: 889 1629 43
Fragments arranged by size:
1630 1629 1425 889 275 201 54 50
43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3676 1537
Size: 275 219 1645 4090
Fragments arranged by size:
4090 1645 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2631 3027 3226 4159 1353
Size: 1278 396 199 933 3423
Fragments arranged by size:
3423 1278 933 396 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3303 4816 5190 5336 387
Size: 1064 138 1714 1513 374 146 1280
Fragments arranged by size:
1714 1513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2777 3301 3315 4292 4889 5676 5944
Size: 393 1145 524 14 977 597 787 268
Cuts at: 5944 1239
Size: 1524
Fragments arranged by size:
1524 1145 977 787 597 524 393 268
14
EcoO109I rG'GnC_Cy
Cuts at: 2584 2584
Size: 6229
EcoRI G'AATT_C
Cuts at: 2265 2319 2967 3538 3916 2265
Size: 54 648 571 378 4578
Fragments arranged by size:
4578 648 571 378 54
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2025 3171 5376 1678
Size: 347 1146 2205 2531
Fragments arranged by size:
2531 2205 1146 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2480 2512 4870
Size: 32 135 32 512 32 364 32 2358
Cuts at: 4870 4902 4944 4976 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2358 512 364 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 6229
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2356 2691 5343 5527 1305
Size: 1051 335 2652 184 2007
Fragments arranged by size:
2652 2007 1051 335 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4407
Fragments arranged by size:
4407 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2597 4187 4546 4705 518
Size: 648 1431 1590 359 159 2042
Fragments arranged by size:
2042 1590 1431 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3228 3645 4322 5470 5715 1606
Size: 1622 417 677 1148 245 2120
Fragments arranged by size:
2120 1622 1148 677 417 245
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 6229
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5677
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6229
NheI G'CTAG_C
Cuts at: 1052 2899 1052
Size: 1847 4382
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NruI TCG'CGA
Cuts at: 2042 2042
Size: 6229
NsiI A_TGCA'T
Cuts at: 2343 2343
Size: 6229
NspI r_CATG'y
Cuts at: 1132 1229 3587 5132 1132
Size: 97 2358 1545 2229
Fragments arranged by size:
2358 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6229
PciI A'CATG_T
Cuts at: 5128 5128
Size: 6229
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5327
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2584 2584
Size: 6229
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 2718 3132 3799 2718
Size: 414 667 5148
Fragments arranged by size:
5148 667 414
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2217 2249 2217
Size: 32 6197
PstI C_TGCA'G
Cuts at: 830 2302 2581 3581 830
Size: 1472 279 1000 3478
Fragments arranged by size:
3478 1472 1000 279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SfcI C'TryA_G
Cuts at: 826 1045 2201 2223 2298 2577 3577 4712 5393
Size: 219 1156 22 75 279 1000 1135 681
Cuts at: 5393 5584 826
Size: 191 1471
Fragments arranged by size:
1471 1156 1135 1000 681 279 219 191
75 22
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 6229
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6229
SmaI CCC'GGG
Cuts at: 1872 3556 1872
Size: 1684 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2796 3471 4333 5234 5496 820
Size: 197 926 853 675 862 901 262 1553
Fragments arranged by size:
1553 926 901 862 853 675 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6229
StyI C'CwwG_G
Cuts at: 513 1065 2271 3668 513
Size: 552 1206 1397 3074
Fragments arranged by size:
3074 1397 1206 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2494 4256 4273 4426 4611 5056 325
Size: 1255 914 1762 17 153 185 445 1498
Fragments arranged by size:
1762 1498 1255 914 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3214 4475 6187
Size: 274 80 33 51 1443 1237 1261 1712
Cuts at: 6187 96
Size: 138
Fragments arranged by size:
1712 1443 1261 1237 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6189 6221 6189
Size: 32 6197
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3028 3565 3028
Size: 537 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3523 3523
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 2796 1943
Size: 853 5376
XmaI C'CCGG_G
Cuts at: 1870 3554 1870
Size: 1684 4545
XmnI GAAnn'nnTTC
Cuts at: 2293 4358 2293
Size: 2065 4164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4037 276
Size: 53 83 186 936 2503 2468
Fragments arranged by size:
2503 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV PacI PfoI PmlI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI